Phoenixin-14 may ameliorate testicular damage caused by torsion-detorsion by reducing oxidative stress and inflammation in prepubertal rats

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dc.authoridYilmaz, Nesibe/0000-0002-5527-8507
dc.authorwosidYilmaz, Nesibe/JFK-5834-2023
dc.contributor.authorYilmaz, Nesibe
dc.contributor.authorHudaykuliyeva, Jemal
dc.contributor.authorGul, Semir
dc.date.accessioned2024-08-04T21:00:10Z
dc.date.available2024-08-04T21:00:10Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe present study aimed to investigate the effects of Phoenixin-14 (PNX-14) on oxidative damage, inflammatory response, histopathological variations, and serum testosterone levels in testicular tissues. Forty-eight Wistar albino prepubertal male rats were divided into 4 groups (Sham, TTD, TT +PNX +TD, TTD +PNX) (n =12). The torsion period was 2 hours and the detorsion period was 24 hours in the testicular torsion/detorsion (TD) groups. A single PNX-14 (50 mu g/kg) dose was injected into the rats in the TT +PNX TD group on the 90th minute of torsion, and it was injected into the rats in the TTD +PNX group at the beginning of detorsion. Oxidative damage in testicular tissues was determined based on superoxide dismutase (SOD), malondialdehyde (MDA), total antioxidant status (TAS) and total oxidant status (TOS), and inflammatory damage was determined based on TNF- alpha and IL -6 levels. Histopathological variations were investigated with the Periodic Acid Schiff (PAS) staining method in testicular tissues and analyzed based on Johnsen scores. Spermatogonia cells were examined immunohistochemically. Serum testosterone levels were determined with the enzyme -linked immunosorbent assay (ELISA). A significant increase in oxidative stress and inflammation parameters was determined in the TTD group when compared to the other groups (p <0.05). PNX-14 treatment led to a statistically significant decrease in these parameters and significantly repaired the TD damage in testicular tissue (p <0.05). Johnsen scoring revealed significant improvement in PNX-14 groups and an increase in spermatogonia count, supporting the biochemical findings (p <0.05). PNX-14 could be a potential therapeutic agent in testicular TD damage and further studies should be conducted to elucidate the present study findings.en_US
dc.description.sponsorshipKarabuk University Karabuk University Scientific Research Projects Department [KBBAP-22-YL-112]en_US
dc.description.sponsorshipThis work supported by the Karabuk University Karabuk University Scientific Research Projects Department (Project No: KBUBAP-22-YL-112)en_US
dc.identifier.doi10.1016/j.tice.2024.102405
dc.identifier.issn0040-8166
dc.identifier.pmid38754242en_US
dc.identifier.urihttps://doi.org/10.1016/j.tice.2024.102405
dc.identifier.urihttps://hdl.handle.net/11616/103851
dc.identifier.volume88en_US
dc.identifier.wosWOS:001244400100001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherChurchill Livingstoneen_US
dc.relation.ispartofTissue & Cellen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTorsion/detorsionen_US
dc.subjectPhoenixin-14en_US
dc.subjectOxidative stressen_US
dc.subjectInflammationen_US
dc.subjectSpermatogonia morphologyen_US
dc.titlePhoenixin-14 may ameliorate testicular damage caused by torsion-detorsion by reducing oxidative stress and inflammation in prepubertal ratsen_US
dc.typeArticleen_US

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