SREBP-1c Deficiency Attenuates Fructose-Induced Lipid Droplet Accumulation

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dc.contributor.authorMese-Tayfur, Seher
dc.contributor.authorIsot, Ibrahim
dc.contributor.authorCetinkaya, Bengue
dc.contributor.authorYalciner, Tugce Demirel
dc.contributor.authorOezer, Nesrin Kartal
dc.contributor.authorSoezen, Erdi
dc.date.accessioned2026-04-04T13:31:19Z
dc.date.available2026-04-04T13:31:19Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractObjective: Sterol regulatory element binding protein 1c (SREBP-1c), a transcription factor involved in the biosynthesis of fatty acids, is critical in metabolic dysfunction-associated fatty liver disease (MAFLD) by promoting lipid accumulation and metabolic dysregulation that leads to hepatic pathologies. Fructose, becoming increasingly common in diets, activates SREBP-1c by increasing acetyl-CoA production. Present study aimed to sought the effect of SREBP-1c in fructose induced lipid accumulation. Methods: A fructose-induced lipid accumulation model was developed in mouse hepatocyte cells (AML12), where SREBP-1c expression was inhibited through siRNA transfection. Following different fructose concentrations, viability was determined by MTT assay, and the protein expression of SREBP-1c protein was determined by western blotting. The number of lipid droplets (LDs) was quantified microscopically, and lipogenic mRNA expressions of FASN, SCD1, GPAM, ACLY, ACSL1 and ACACA were detected by qRT-PCR. Results: Western blotting and microscopic analysis indicated that 25 mM for 72 hours of fructose increased total LDs, together with SREBP-1c levels, without affecting cell viability. The mRNA expression of SREBP-1c decreased in the presence of siRNA, confirming siRNA efficacy. SREBP-1c silencing reduced the number of fructose-induced total LDs. As lipogenic mRNA expressions, SREBP-1c silencing reduced SCD1 and ACLY, while other genes were unaffected. Conclusion: Silencing of SREBP-1c in hepatocytes demonstrated its beneficial effect by reducing fructose-induced LD accumulation.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [323S171]
dc.description.sponsorshipFunding: This work was supported by the The Scientific and Technological Research Council of Turkey (TUBITAK) [grant number 323S171] .
dc.identifier.doi10.33808/clinexphealthsci.1603452
dc.identifier.endpage216
dc.identifier.issn2459-1459
dc.identifier.issue1
dc.identifier.orcid0000-0003-3487-7065
dc.identifier.orcid0000-0002-6046-7679
dc.identifier.orcid0009-0009-4705-2995
dc.identifier.orcid0009-0000-9857-742X
dc.identifier.startpage210
dc.identifier.urihttps://doi.org/10.33808/clinexphealthsci.1603452
dc.identifier.urihttps://hdl.handle.net/11616/108713
dc.identifier.volume15
dc.identifier.wosWOS:001500914100031
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherMarmara Univ, Inst Health Sciences
dc.relation.ispartofClinical and Experimental Health Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectSterol regulatory element binding transcription factor 1c
dc.subjectfructose
dc.subjectlipid accumulation
dc.titleSREBP-1c Deficiency Attenuates Fructose-Induced Lipid Droplet Accumulation
dc.typeArticle

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