Investigation of Beta-Lactamase Genes and Clonal Relationship Among the Extended-Spectrum Beta-Lactamase Producing Nosocomial Escherichia coli Isolates

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dc.authoridmemişoğlu, funda/0000-0003-3905-1182
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridErsoy, Yasemin/0000-0001-5730-6682
dc.authorwosidmemişoğlu, funda/AAA-4392-2021
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.authorwosidErsoy, Yasemin/AAE-4811-2020
dc.contributor.authorGorgec, Sunduz
dc.contributor.authorKuzucu, Cigdem
dc.contributor.authorOtlu, Baris
dc.contributor.authorYetkin, Funda
dc.contributor.authorErsoy, Yasemin
dc.date.accessioned2024-08-04T20:59:41Z
dc.date.available2024-08-04T20:59:41Z
dc.date.issued2015
dc.departmentİnönü Üniversitesien_US
dc.description.abstractExtended-spectrum beta-lactamase (ESBL) producing microorganisms currently cause a major problem. Among theseCTX-M beta-lactamase producing Escherichia coli has also disseminated worldwide as an important cause of both nosocomial and community-acquired infections. The aims of this study were to determine the prevalence of the beta-lactamase genes, antibiotic susceptibilities and clonal relationships of ESBL-producing nosocomial E.coli isolates. A total of 76 ESBL-producing E.coli strains isolated from urine (n= 26), blood (n= 25) and wound (n= 25) specimens of hospitalized patients identified as nosocomial infection agents according to the CDC criteria between June 2010-June 2011 were included in the study. Antibiotic susceptibilities of the isolates were detected by Kirby-Bauer disc diffusion method according to CLSI recommendations. ESBL production was tested by double disc diffusion method, and cefotaxime/cefotaxime-clavulanic acid E-test strips (AB Biodisk, Sweden) were used for indeterminate results. Presence of TEM, SHV, CTX-M, OXA-2 group, OXA-10 group, PER, VEB and GES beta-lactamase genes were investigated by polymerase chain reaction (PCR) using specific primers. Pulsed-field gel electrophoresis (PFGE) method was used for the detection of clonal relationships among the strains. Most of the ESBL-producing E.coli strains were isolated from samples of inpatients in intensive care (35%), internal medicine (16%) and general surgery (13%) units. All of the 76 strains were found susceptible to imipenem, meropenem and amikacin; however all were resistant to cefotaxime and ceftriaxone. The susceptibility rates of the isolates to cefoxitin, ertapenem, cefoperazone/sulbactam, piperacillin-tazobactam, gentamicin, ciprofloxacin, cefepime, amoxicillin-clavulanic acid, aztreonam and ceftazidime were 96%, 83%, 63%, 61%, 50%, 41%, 25%, 21%, 20% and 18%, respectively. Among E.coli isolates, the frequency of CTX-M, TEM, OXA-2 group, PER, SHV and OXA-10 group beta-lactamase genes were found as 89.5%, 59.2%, 15.8%, 14.5%, 11.8% and 3.9%, respectively, while none of the isolates were positive for VEB and GES beta-lactamase genes. In 1 (1.3%) strain none of the investigated genes were detected. PCR analyses of the isolates revealed that 25 harbored CTX-M and TEM genes together, while 20 harbored only CTX-M and two harbored only TEM genes. Single SHV gene was not detected in any of the isolates. PFGE demonstrated no major clonal relationship between ESBL-producing isolates. This study indicated that CTX-M type enzymes were highly endemic among ESBL-producing nosocomial E.coli strains in our hospital, with the polyclonal spread of ESBL-producing bacteria without any dominant epidemic clone. In conclusion, it was considered that further studies are needed to explain the relationship between epidemic clones and plasmids with the use of plasmid analysis and multilocus sequence typing methods.en_US
dc.identifier.endpage25en_US
dc.identifier.issn0374-9096
dc.identifier.issue1en_US
dc.identifier.pmid25706727en_US
dc.identifier.startpage15en_US
dc.identifier.urihttps://hdl.handle.net/11616/103446
dc.identifier.volume49en_US
dc.identifier.wosWOS:000350946600002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEscherichia colien_US
dc.subjectbeta-lactamaseen_US
dc.subjectCTX-Men_US
dc.subjectclonal relationshipen_US
dc.titleInvestigation of Beta-Lactamase Genes and Clonal Relationship Among the Extended-Spectrum Beta-Lactamase Producing Nosocomial Escherichia coli Isolatesen_US
dc.typeArticleen_US

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