Glioblastoma cell-derived exosomes induce cell death and oxidative stress in primary cultures of olfactory neurons. Role of redox stress

Basit Öğe Kaydı
dc.authoridBolat, Ismail/0000-0003-1398-7046
dc.authoridYeni, Yeşim/0000-0002-6719-7077
dc.authoridTAGHIZADEHGHALEHJOUGHI, Ali/0000-0002-3506-0324
dc.authorwosidBolat, Ismail/AAU-9698-2021
dc.authorwosidHacimuftuoglu, Ahmet/U-9109-2018
dc.authorwosidYeni, Yeşim/JTV-4518-2023
dc.authorwosidGenç, Sıdıka/AHB-6098-2022
dc.authorwosidYildirim, Serkan/AAH-6721-2020
dc.contributor.authorYeni, Yesim
dc.contributor.authorTaghizadehghalehjoughi, Ali
dc.contributor.authorGenc, Sidika
dc.contributor.authorHacimuftuoglu, Ahmet
dc.contributor.authorYildirim, Serkan
dc.contributor.authorBolat, Ismail
dc.date.accessioned2024-08-04T20:58:53Z
dc.date.available2024-08-04T20:58:53Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackgroundGlioblastoma multiforme, described as glioblastoma, is a malignancy originating from glial progenitors in the central nervous system and is the most malignant subtype of brain tumors which attracted researcher's attention due to their high recurrence and mortality despite optimal treatments. In the study, we aimed to research whether glioblastoma-originated exosomes play a role in olfactory nerve cell toxicity.Methods and resultsFor this aim, exosomes obtained from U373 and T98G cells were applied to olfactory nerve cell culture at distinct doses. Then, glutathione (GSH), lactate dehydrogenase (LDH), total antioxidant capacity (TAC), 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT), total oxidant status (TOS) and Immunofluorescence analyzes were performed. We found that both glioblastoma-derived exosomes decreased cell viability in olfactory neurons with increasing doses. According to the obtained data, the olfactory neuron vitality rate was 71% in T98G-exosome, but the decrease in U373-exosome was more obvious (48%). In particular, the 100 mu g/ml dose exacerbated oxidative stress by increasing TOS. It also increased cellular apoptosis compared to the control group due to LDH leakage. However, the results of GSH and TAS showed that antioxidant levels were significantly reduced.ConclusionIn the microenvironment of olfactory neurons, GBM-derived exosomes increased oxidative stress-induced toxicity by reducing TAC and GSH levels. Therefore, glioblastoma cells by induction of exosome-based stress support malignant growth.en_US
dc.identifier.doi10.1007/s11033-023-08256-0
dc.identifier.endpage4009en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue5en_US
dc.identifier.pmid36849859en_US
dc.identifier.startpage3999en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-023-08256-0
dc.identifier.urihttps://hdl.handle.net/11616/103266
dc.identifier.volume50en_US
dc.identifier.wosWOS:000940303500005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectExosomeen_US
dc.subjectGlioblastomaen_US
dc.subjectOlfactoryen_US
dc.subjectNeurotoxicityen_US
dc.titleGlioblastoma cell-derived exosomes induce cell death and oxidative stress in primary cultures of olfactory neurons. Role of redox stressen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu