Effects of Alpha-Lipoic Acid on TGF-?1 and Urotensin-II Levels in Glucocorticoid-Induced Osteonecrosis in Rats

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dc.authoridDokuyucu, Recep/0000-0001-6837-3477
dc.authoridDokuyucu, Recep/0000-0001-6837-3477
dc.authoridKoca, İrfan/0000-0003-1257-6843
dc.authoridGokce, Hasan/0000-0002-4658-9987
dc.authorwosidDokuyucu, Recep/A-5201-2014
dc.authorwosidDokuyucu, Recep/GUI-2377-2022
dc.authorwosidTaş, Zeynel Abidin/AFD-7893-2022
dc.authorwosidDokuyucu, Recep/JWO-9276-2024
dc.authorwosidKoca, İrfan/ABN-6241-2022
dc.authorwosidGokce, Hasan/ABH-2400-2020
dc.contributor.authorKoca, Irfan
dc.contributor.authorDokuyucu, Recep
dc.contributor.authorTas, Zeynel Abidin
dc.contributor.authorGokce, Hasan
dc.contributor.authorOzcan, Oguzhan
dc.date.accessioned2024-08-04T20:58:31Z
dc.date.available2024-08-04T20:58:31Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground: Osteonecrosis (ON) is a serious health problem, which dramatically reduces the quality of life. Objectives: In the present study on the rat model of glucocorticoids (GCs) -induced ON, we explored the influence of alpha-lipoic acid on serum levels of TGF-fi1 and urotensin-II (U-II) and on histological alteration with respect to fatty degeneration and osteocyte necrosis. Methods: A total of 32 male Wistar albino rats were equally assigned to four groups, including control, methylprednisolone acetate (MPA), alpha-lipoic acid (ALA), and MPA with ALA (MP1 + ALA). The animals in MPA group subcutaneously received 15 mg/kg/week during 2 weeks, whereas 100 mg/kg/day ALA was intraperitoneally administered to ALA group during 4 weeks. The MPA + ALA group had both treatments with the same doses. ON was confirmed and graded histologically. Lipid peroxidation and DNA damage levels were immunohistochemically assessed in rats' bones. Results: After histopathological examinations, ALA injection attenuated oxidative stress levels through reducing both 8-OHdG-and 4-HNE-positive cells in the femoral head region (P < 0.05). The U-II and TGF-fi1 protein levels significantly decreased after ALA treatment in MPA injected animals (P < 0.05, P < 0.01, respectively). Moreover, there was a strong correlation between U-II and TGF-fi 1 protein levels (P = 0.019, r = 0.884). Conclusions: This study is novel with regard to showing the therapeutic effects of ALA on GC-inducedONin rats as well as the strong correlation between the expression levels of U-II and TGF-fi1 proteins. In this regard, ALAmaybe a therapeutic agent in the treatment of ON patients.en_US
dc.identifier.doi10.5812/ircmj.97279
dc.identifier.issn2074-1804
dc.identifier.issn2074-1812
dc.identifier.issue4en_US
dc.identifier.urihttps://doi.org/10.5812/ircmj.97279
dc.identifier.urihttps://hdl.handle.net/11616/102930
dc.identifier.volume22en_US
dc.identifier.wosWOS:000532374500004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherKowsar Publen_US
dc.relation.ispartofIranian Red Crescent Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOsteonecrosisen_US
dc.subjectAlpha-Lipoic Aciden_US
dc.subjectUrotensin-IIen_US
dc.subjectTGF-beta 1en_US
dc.titleEffects of Alpha-Lipoic Acid on TGF-?1 and Urotensin-II Levels in Glucocorticoid-Induced Osteonecrosis in Ratsen_US
dc.typeArticleen_US

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