Comparison of an automated system with four phenotypic methods for the detection of methicillin-resistant Staphylococcus aureus

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dc.authoridTekerekoglu, Mehmet Sait/0000-0001-7284-3427
dc.authoridAy, selma/0000-0002-7640-6635
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authorwosidTekerekoglu, Mehmet Sait/ABI-8148-2020
dc.authorwosidAy, selma/AAA-7021-2021
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.contributor.authorIraz, Meryem
dc.contributor.authorTekerekoglu, Mehmet Sait
dc.contributor.authorOtlu, Baris
dc.contributor.authorAy, Selma
dc.date.accessioned2024-08-04T20:57:27Z
dc.date.available2024-08-04T20:57:27Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractCorrect and rapid detection of methicillin resistance in Staphylococcus aureus is very important for treatment of infected patients. Detection of the mecA gene or PBP2a by polymerase chain reaction (PCR) is considered the gold standard for determination of methicillin resistance in staphylococci. In most clinical laboratories, phenotypic methods are used for the detection of methicillin resistance in S. aureus because PCR is not suitable for routine usage. In this study, we aimed to compare different phenotypic methods: disk diffusion, agar screening, latex agglutination and an automated system employed to establish the presence of methicillin-resistant S. aureus (MRSA). Presence of the mecA gene via PCR was used as the marker for MRSA positivity. Afterward, 214 samples were analyzed for methicillin resistance via oxacillin or cefoxitin disk diffusions, oxacillin agar screening, MRSA latex agglutination and the automated BD Phoenix system. Sensitivity, specificity, negative and positive predictive values of these phenotypic methods were evaluated. In the detection of MRSA, the cefoxitin disk-diffusion method was found to be more useful than oxacillin disk diffusion. The automated MRSA strain-detection system was found to be more successful than the other phenotypic methods. These results showed that the automated system could be used safely for routine MRSA detection.en_US
dc.identifier.doi10.5897/AJMR11.903
dc.identifier.endpage769en_US
dc.identifier.issn1996-0808
dc.identifier.issue4en_US
dc.identifier.startpage764en_US
dc.identifier.urihttps://doi.org/10.5897/AJMR11.903
dc.identifier.urihttps://hdl.handle.net/11616/102641
dc.identifier.volume6en_US
dc.identifier.wosWOS:000306480900009en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.relation.ispartofAfrican Journal of Microbiology Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPhoenixen_US
dc.subjectcefoxitin disk diffusionen_US
dc.subjectmecAen_US
dc.subjectoxacillinen_US
dc.subjectmethicillin-resistant Staphylococcus aureusen_US
dc.subjectlatex agglutinationen_US
dc.titleComparison of an automated system with four phenotypic methods for the detection of methicillin-resistant Staphylococcus aureusen_US
dc.typeArticleen_US

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