Infection of dendritic cells by a gamma 2 herpesvirus induces functional modulation
Yükleniyor...
Dosyalar
Tarih
2005
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Journal of immunology
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The murine γ-herpesvirus-68 (γHV68) establishes viral latency in dendritic cells (DCs). In the
present study, we examined the specific consequences of DC infection by γHV68, both in vivo and
in vitro. Ex vivo analysis of infected mice showed that the virus colonizes respiratory DCs very
early after infection and that all subsets of splenic DCs analyzed are viral targets. We have
developed and characterized an in vitro model of γHV68 infection of DCs. Using this model, we
demonstrated that viral infection neither induces full DC maturation nor interferes with exogenous
activation, which is assessed by cell surface phenotypic changes. However, whereas γHV68
infection alone failed to elicit cytokine secretion, IL-10 secretion of exogenously activated DCs
was enhanced. Furthermore, γHV68-infected DCs efficiently stimulated virus-specific T cell
hybridomas but failed to induce alloreactive stimulation of normal T cells. These data indicate that
viral infection doesn’t interfere with Ag processing and presentation but does interfere with the
ability of DCs to activate T cells. The inhibition of T cell activation was partially reversed by
blocking IL-10. Analysis of infected mice shows elevated levels of IL-10 expression in DCs and
that lack of endogenous IL-10 is associated with decreased γHV68 long-term latency. Taken
together, these observations indicate that γ2-herpesvirus infection of DCs is a mechanism of viral
immune evasion, partially mediated by IL-10.
Açıklama
Anahtar Kelimeler
Kaynak
Journal of immunology
WoS Q Değeri
Scopus Q Değeri
Cilt
175
Sayı
5
Künye
Flano, E. Kayhan, B. Woodland, D. Blackman, M. (2005). Infection of dendritic cells by a gamma 2 herpesvirus induces functional modulation. Journal of immunology. 175(5), 3225–3234.
