Investigation of carbapenem resistance and the first identification of Klebsiella pneumoniae carbapenemase KPC enzyme among Escherichia coli isolates in Turkey A prospective study

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dc.authorid101949en_US
dc.contributor.authorKuşkucu, Mert Ahmet
dc.contributor.authorKarakullukçu, Asiye
dc.contributor.authorAiliken, Maılıhaba
dc.contributor.authorOtlu, Barış
dc.contributor.authorMete, Birgül
dc.date.accessioned2017-07-10T07:14:27Z
dc.date.available2017-07-10T07:14:27Z
dc.date.issued2016
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground: The aim of this study was to determine the presence of carbapenem resistance and carbapenemase production in Escherichia coli isolates from clinical samples in Turkey. Methods: The prospective study included a total of 4.052 Escherichia coli isolates collected from patients admitted to a hospital from March 2011 to May 2012. We used ertapenem disc for screening carbapenemase production, and the confirmation was performed by using Etest. The resistance mechanisms and genetic relatedness of the carbapenem resistant strains were investigated by using PCR (polymerase chain reaction) and pulsed-field gel electrophoresis (PFGE), respectively. Results: Among the 4.052 E. coli isolates, 24 (0.59%) were found to be carbapenem resistant. Of these, only 5 isolates were positive for OXA-48 and 2 isolates were positive for Klebsiella pneumoniae carbapenemase (KPC)-2. The KPC-2 producing E. coli strains (n ¼ 2) were both isolated from the same patient. The blaKPC genes were confirmed using DNA sequence analysis. The genetic relationship between the 24 E. coli strains studied by PFGE revealed that the strains were genetically unrelated. Conclusions: This article confirms, to our knowledge for the first time, the detection of KPC-2-producing E. coli in Turkey, with OXA-48 being the most frequent carbapenemase in the study.en_US
dc.identifier.citationKuşkucu, M. A. Karakullukçu, A. Ailiken, M. Otlu, B. Mete, B. (2016). Investigation of carbapenem resistance and the first identification of Klebsiella pneumoniae carbapenemase KPC enzyme among Escherichia coli isolates in Turkey A prospective study. Travel Medicine and Infectious Disease, 14(6), 572–576.en_US
dc.identifier.doi10.1016/j.tmaid.2016.11.006en_US
dc.identifier.endpage576en_US
dc.identifier.issn14778939
dc.identifier.issue6en_US
dc.identifier.startpage572en_US
dc.identifier.urihttp://linkinghub.elsevier.com/retrieve/pii/S1477893916301727
dc.identifier.urihttps://hdl.handle.net/11616/7349
dc.identifier.volume14en_US
dc.language.isoenen_US
dc.publisherTravel Medicine and Infectious Diseaseen_US
dc.relation.ispartofTravel Medicine and Infectious Diseaseen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEscherichia colien_US
dc.subjectCarbapenem resistanceen_US
dc.subjectCarbapenemaseen_US
dc.subjectKlebsiella pneumoniae carbapenemaseen_US
dc.subjectOXA-48en_US
dc.titleInvestigation of carbapenem resistance and the first identification of Klebsiella pneumoniae carbapenemase KPC enzyme among Escherichia coli isolates in Turkey A prospective studyen_US
dc.typeArticleen_US

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