Effects of Recombinant Activated Protein C Derived From Drotrecogin-Alpha on Bleomycin-Induced Pulmonary Fibrosis in Rats Compared with Methyl-Prednisolone

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dc.authoridTurkmen, Samdanci, Emine/0000-0002-0034-5186
dc.authoridHacievliyagil, Suleyman Savas/0000-0002-2572-1870
dc.authoridAytemur, Zeynep Ayfer/0000-0003-0421-907X
dc.authorwosidTurkmen, Samdanci, Emine/ABH-4716-2020
dc.authorwosidHacievliyagil, Suleyman Savas/ABI-7307-2020
dc.authorwosidAytemur, Zeynep Ayfer/ABI-7452-2020
dc.contributor.authorYildiz, Kadir
dc.contributor.authorIraz, Mustafa
dc.contributor.authorSamdanci, Emine
dc.contributor.authorOzerol, Elif
dc.contributor.authorKuku, Irfan
dc.contributor.authorAytemur, Zeynep Ayfer
dc.contributor.authorHacievliyagil, Suleyman Savas
dc.date.accessioned2024-08-04T20:58:34Z
dc.date.available2024-08-04T20:58:34Z
dc.date.issued2013
dc.departmentİnönü Üniversitesien_US
dc.description.abstractOBJECTIVE: In this study, we aimed to test the preventive effects of intraperitoneally administered drotrecogin alpha which is derived from activated protein C (APC), on bleomycin-induced pulmonary fibrosis in rats, and to compare the effects of APC with the effects of methyl-prednisolone, a traditional therapy. MATERIAL AND METHODS: Thirty male Wistar albino rats were randomly allocated into four groups: 1. Saline alone (n= 6); 2. Bleomycin+ placebo (n= 7); 3. Bleomycin+ methyl-prednisolone (n= 7); 4. Bleomycin+ APC (n= 10). The rats (except for the control group) were given intratracheal bleomycin (2.5 mg/kg). The bleomycin+ APC group was given APC (100 mu g/kg/day) and methyl-prednisolone treated rats were injected with 5mg/kg/day methyl-prednisolone intraperitoneally two days before the bleomycin injection; the drug was administered at the same dose for 16 days. All of the rats were killed 14 days after the intratracheal injection of bleomycin. Fibrotic changes in the lungs were demonstrated by analysing the cellular composition of bronchoalveolar lavage fluid, histological evaluation and lung hydroxyproline content. RESULTS: Fibrosis was experimentally induced in the lungs of rats using bleomycin. Fibrosis scores in the bleomycin+ methyl-prednisolone and the bleomycin+ APC groups were significantly lower than in the bleomycin+ placebo group (p< 0.05). The scores of the bleomycin+ APC group and the bleomycin+ methyl-prednisolone group were similar. The lung tissue hydroxyproline contents in the bleomycin+ placebo and bleomycin+ methyl-prednisolone groups were significantly higher than the control group (p< 0.05), but the hydroxyproline content in the bleomycin+ APC group was significantly lower than in the other groups (p< 0.05). CONCLUSION: Drotrecogin alpha that is derived from recombinant APC has a protective effect on the pulmonary fibrosis induced by bleomycin. The protective effect seen with methylprednisolone is similar.en_US
dc.identifier.doi10.5152/ttd.2013.10
dc.identifier.endpage52en_US
dc.identifier.issn1302-7808
dc.identifier.issn1308-5387
dc.identifier.issue2en_US
dc.identifier.startpage48en_US
dc.identifier.urihttps://doi.org/10.5152/ttd.2013.10
dc.identifier.urihttps://hdl.handle.net/11616/102984
dc.identifier.volume14en_US
dc.identifier.wosWOS:000421761000003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isotren_US
dc.publisherBilimsel Tip Publishing Houseen_US
dc.relation.ispartofTurkish Thoracic Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBleomycinen_US
dc.subjectpulmonary fibrosisen_US
dc.subjectrecombinant activated protein Cen_US
dc.subjectmethyl-prednisoloneen_US
dc.subjectraten_US
dc.titleEffects of Recombinant Activated Protein C Derived From Drotrecogin-Alpha on Bleomycin-Induced Pulmonary Fibrosis in Rats Compared with Methyl-Prednisoloneen_US
dc.typeArticleen_US

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