Effect of resveratrol and melatonin on oxidative stress enzymes, regeneration, and hepatocyte ultrastructure in rats subjected to 70% partial hepatectomy

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dc.authoridYilmaz, Sezai/0000-0002-8044-0297
dc.authoridECEVIT, ATA NIYAZI/0000-0002-8820-9305
dc.authoridbay karabulut, aysun/0000-0002-7873-2805;
dc.authorwosidYilmaz, Sezai/ABI-2323-2020
dc.authorwosidECEVIT, ATA NIYAZI/ACQ-5909-2022
dc.authorwosidbay karabulut, aysun/HJP-0995-2023
dc.authorwosidEcevit, Ata Niyazi/Z-1436-2019
dc.contributor.authorKirimlioglu, H.
dc.contributor.authorEcevit, A.
dc.contributor.authorYilmaz, S.
dc.contributor.authorKirimlioglu, V.
dc.contributor.authorKarabulut, A. Bay
dc.date.accessioned2024-08-04T20:58:30Z
dc.date.available2024-08-04T20:58:30Z
dc.date.issued2008
dc.departmentİnönü Üniversitesien_US
dc.descriptionAnnual Meeting of the Turkish-Transplantation-Society -- JUL 04-06, 2007 -- Ankara, TURKEYen_US
dc.description.abstractAim. We sought to compare the antioxidant effects of resveratrol (R) and melatonin (M) after 70% partial hepatectomy (PH) as evidenced by ultrastructural alterations and effects on hepatocyte proliferation and apoptosis. Methods. Twenty-six male Wistar albino rats were randomized into four groups: group A (n = 8) resveratrol (R); group B (n = 8) melatonin (M); group C (n = 5) control PH; group D (n = 5) sham operated animals. The rats that received either R or M were sacrificed a week after PH. The malondialdehyde, glutathione, glutathione S-transferase, and nitric oxide levels were estimated in liver homogenates. The morphological changes were investigated using light and electron microscopy (EM). Cell proliferation was detected by immunohistochemical staining with monoclonal antibodies to Ki-67. Apoptosis was detected by the transferase-mediated dUTP nick end-labeling method. Results. PH induced hepatic LP, decreased GSH and NO, and inhibited GST activity (P < .05). R and M completely prevented PH-induced lipid peroxidation, decreased hepatic GSH and NO levels (P < .05). The inhibition of GST activity was prevented by R (P < .05), but not with M (P > .05). In the PH group EM showed severe morphological changes: mitochondrial degeneration, vacuoles, lipid droplets, and myelin-like figures. In both the R and M groups, morphological alterations repaired protective effects more prominently in the R group. Ki-67 indices (KI) were increased in the PH group and decreased in both R and M groups (P < .001). In the M group, KI was the lowest, but the difference compared with R was not significant (P > .05). Apoptosis was slightly increased in PH, but in either the R or M groups, apoptosis was intensively increased (P < .001). Increased apoptosis was greatest in the M group and the difference compared with the R group was statistically significant (P < .05). Conclusion. R and M suppressed PH-induced oxidative damage, attenuated proliferation, and stimulated apoptosis. When we compared R and M, R showed more potent antioxidative effects and was morphologically more protective to hepatocytes. Antiproliferative effects of M were more potent. Because of their potent antioxidative effects, R and M can be effective for oxidative damage like ischemia-reperfusion injury; however, because of the adverse effects on proliferation and apoptosis more studies are needed in states in which regeneration is critical.en_US
dc.description.sponsorshipTurkish Transplantat Socen_US
dc.identifier.doi10.1016/j.transproceed.2007.11.050
dc.identifier.endpage289en_US
dc.identifier.issn0041-1345
dc.identifier.issue1en_US
dc.identifier.pmid18261607en_US
dc.identifier.startpage285en_US
dc.identifier.urihttps://doi.org/10.1016/j.transproceed.2007.11.050
dc.identifier.urihttps://hdl.handle.net/11616/102912
dc.identifier.volume40en_US
dc.identifier.wosWOS:000253229500073en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTransplantation Proceedingsen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBreast-Cancer Cellsen_US
dc.subjectAcid Induces Apoptosisen_US
dc.subjectLipid-Peroxidationen_US
dc.subjectLiver-Injuryen_US
dc.subjectMiceen_US
dc.subjectPolyphenolsen_US
dc.subjectRegimenen_US
dc.subjectTumoren_US
dc.titleEffect of resveratrol and melatonin on oxidative stress enzymes, regeneration, and hepatocyte ultrastructure in rats subjected to 70% partial hepatectomyen_US
dc.typeConference Objecten_US

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