Retinal nitric oxide and malonyldialdehyde levels following photodynamic therapy

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dc.authoridIlhan, Nevin/0000-0002-0208-8929
dc.authoridÖztürkmen, Cem/0000-0003-3720-7112
dc.authorwosidturkcuoglu, peykan/GQB-2596-2022
dc.authorwosidIlhan, Nevin/J-5761-2017
dc.authorwosidAydemir, Orhan/W-1452-2018
dc.authorwosidÖztürkmen, Cem/ABG-3827-2021
dc.contributor.authorTurkcuoglu, Peykan
dc.contributor.authorOzturkmen, Cem
dc.contributor.authorIlhan, Nevin
dc.contributor.authorKurt, Julide
dc.contributor.authorAydemir, Orhan
dc.contributor.authorCeliker, Ulku
dc.contributor.authorIbrahim, Mohamed A.
dc.date.accessioned2024-08-04T21:01:02Z
dc.date.available2024-08-04T21:01:02Z
dc.date.issued2011
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground:To determine the retinal nitric oxide (NO) and malonyldialdehyde (MDA) levels following photodynamic therapy (PDT). Materials and Methods:Seven Dutch-belted rabbits received dextrose, while seven others received 2 mg/kg verteporfin infusion over a period of 15 minutes in a dim-lit room. Irradiation to a 1.5 mm diameter intact chorioretinal area in the right eye of verteporfin-infused rabbits, was started 5 minutes after the end of infusion. Three groups were control (dextrose infusion), infusion with verteporfin (left eyes were not irradiated), and irradiation after verteporfin injection (right eyes were irradiated). On the fifth day of the experiment, the eyes were enucleated. The retinas were subsequently frozen and homogenized. Nitrite, a stable end-product of NO and MDA, was measured using the spectrophotometer. Protein concentrations were measured by the Lowry method. Tissue NO and MDA levels were expressed as mu mol/gprt and nmol/mgprt, respectively. Results:The mean retinal NO and MDA levels of the control, infusion, and irradiation groups were 24.67 +/- 6.66, 0.11 +/- 0.02; 45.90 +/- 15.52, 0.21 +/- 0.09; and 84.43 +/- 14.96 mu mol/gprt, 0.58 +/- 0.14 nmol/mgprt, respectively. The mean retinal NO levels were significantly elevated in the infusion and irradiation groups compared with the control group (P:0.004; P:0.001). The mean retinal MDA levels were significantly elevated in the infusion and irradiation groups compared to the control one (P:0.026; P:0.001). Also the mean retinal NO and MDA levels in the irradiation group were found to be significantly higher than the infusion group (P:0.018; P:0.018). Conclusion:Not only PDT, but also verteporfin infusion alone resulted in NO and MDA level increments in the retina, which might be toxic.en_US
dc.identifier.doi10.4103/0301-4738.73705
dc.identifier.endpage8en_US
dc.identifier.issn0301-4738
dc.identifier.issue1en_US
dc.identifier.pmid21157064en_US
dc.identifier.startpage5en_US
dc.identifier.urihttps://doi.org/10.4103/0301-4738.73705
dc.identifier.urihttps://hdl.handle.net/11616/104041
dc.identifier.volume59en_US
dc.identifier.wosWOS:000208741800002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMedknow Publications & Media Pvt Ltden_US
dc.relation.ispartofIndian Journal of Ophthalmologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMalonyldialdehydeen_US
dc.subjectnitric oxideen_US
dc.subjectphotodynamic therapyen_US
dc.subjectretinaen_US
dc.titleRetinal nitric oxide and malonyldialdehyde levels following photodynamic therapyen_US
dc.typeArticleen_US

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