INVESTIGATION OF ANTIOXIDANT ENZYME ACTIVITIES IN WHEAT (TRITICUM AESTIVUM L.) CULTIVARS DEPENDING ON NITRIC OXIDE APPLICATION UNDER CADMIUM STRESS
Küçük Resim Yok
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications (P S P)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Wheat cultivars chosen for their cadmium (Cd) tolerance (Sonmez-2001) and sensitivity (Quality) were grown in Hoagland solution for 5 days and then they were treated with 50, 100 and 200 mu M sodium nitroprusside (SNP), a NO donor, with 9 mM Cd. Samples were taken at 24th and 72nd hours after the administration. In the present study, when examining changes in activities of antioxidant enzymes (SOD, CAT, APX, and GST) which are known as internal defense systems built up by cultivars against Cd stress, these enzymes were observed to pose different responses compared to Cd and Cd+SNP administrations. Malondialdehyde (MDA) concentration in plant tissues were also determine comparatively in order to find out lipid peroxidation. The obtained results showed that Cd stress significantly increased lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione S-transferase (GST) activities. This increase was found to be higher at various rates in different treatments in sensitive cultivar Quality compared to tolerant cultivar Sonmez-2001. Cd+SNP treatments increased antioxidant enzyme activities throughout the applications compared to cadmium. This increase was determined to be significant especially in Quality cultivar and thus substantially reduced Cd toxicity. Some Cd+SNP treatments lead to decline in enzyme activities and decrease in lipid peroxidation. In Sonmez cultivar, Cd+100 mu M SNP treatment decreased catalase activity at 24th and 72nd hours compared to cadmium treatment; Cd+50 mu M SNP reduced GST activity at 72nd hour in both cultivars. Cd+SNP treatments increased MDA levels identified at 24th and 72nd hours in Quality cultivar compared to cadmium treatment; but it led to decrease MDA levels in Sonmez cultivar. This decrease was found as 46.40% in Cd+ 100 mu M SNP treatment and 51.43% in Cd+ 200 mu M SNP treatment at 72nd hour. Based on the decrease in MDA level in Sonmez cultivar, especially at 24th hour, Quality cultivar was found to have higher MDA content than Sonmez cultivar. NO demonstrated a reduced or counteractive effect on the Cd-induced increase in the activities of some typical antioxidant enzymes. These results indicate that SNP has great application potential for improving the growth of plants under heavy metal stress such as Cd toxicity.
Açıklama
Anahtar Kelimeler
Triticum aestivum, Cadmium stress, Nitric oxide, Antioxidant enzymes, MDA
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
27
Sayı
1
