EFFECTS OF SODIUM OCTANOATE, ACYLATED GHRELIN, AND DESACYLATED GHRELIN ON THE GROWTH OF GENETICALLY ENGINEERED ESCHERICHIA COLI

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dc.contributor.authorAydin, Suleyman
dc.contributor.authorErenler, Sebnem
dc.contributor.authorKendir, Yalcin
dc.date.accessioned2024-08-04T20:58:35Z
dc.date.available2024-08-04T20:58:35Z
dc.date.issued2011
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAcylated ghrelin is a 28-amino acid peptide hormone bearing a fatty acid group based on octanoic acid (caprylic acid) at the serine which is located at position 3 and at the N-terminus. If this fatty acid is cleaved from acylated ghrelin, the remaining peptide is referred to as desacylated ghrelin. Free fatty acids (FFAs) can kill or inhibit the growth of bacteria. The purpose of this study was to test this ability using acylated ghrelin, desacylated ghrelin, and sodium octanoate (caprylic acid) as carbon sources for the genetically engineered Escherichia coli strains MK79 and MK57. For this experimental work, minimal medium was modified by replacing glucose with equal concentrations of acylated ghrelin, desacylated ghrelin, or sodium octanoate. Bacterial optical density, viability, alpha-amylase production, plasmid stability and pH of the growth medium were measured during these experiments. The media that allo wed most growth, based on viable cell counts and the OD600 of MK79, was minimal medium, followed by the medium containing desacylated ghrelin or acylated ghrelin, and finally the medium containing sodium octanoate. The same order was observed for MK57. Neither of the strains lost plasmids during the entire course of each experiment. There was also little change in the pH of any of the media used for both strains. These results suggest that sodium octanoate, acylated ghrelin, and desacylated ghrelin, when compared with minimal medium, inhibit Escherichia coli growth. Proliferation was lowest when sodium octanoate was used as the carbon source, followed by acylated ghrelin and desacylated ghrelin. Therefore, the acylated ghrelin found previously in human saliva might help to inhibit pathogenic micro organisms, and acylated ghrelin levels below a critical concentration in saliva could result in an increased risk of oral infection.en_US
dc.identifier.doi10.2478/v10011-011-0020-8
dc.identifier.endpage333en_US
dc.identifier.issn1452-8258
dc.identifier.issue4en_US
dc.identifier.startpage328en_US
dc.identifier.urihttps://doi.org/10.2478/v10011-011-0020-8
dc.identifier.urihttps://hdl.handle.net/11616/102994
dc.identifier.volume30en_US
dc.identifier.wosWOS:000293508800009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherVersitaen_US
dc.relation.ispartofJournal of Medical Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectsodium octanoateen_US
dc.subjectEscherichia colien_US
dc.subjectacylated and desacylated ghrelinen_US
dc.subjectgrowthen_US
dc.subjectalpha-amylaseen_US
dc.titleEFFECTS OF SODIUM OCTANOATE, ACYLATED GHRELIN, AND DESACYLATED GHRELIN ON THE GROWTH OF GENETICALLY ENGINEERED ESCHERICHIA COLIen_US
dc.typeArticleen_US

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