Investigating the Presence of Mycoplasma Hominis-Ureaplasma Urealyticum and in Vitro Antimicrobial Susceptibilities in Patients With Sterile Pyuria
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2014
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info:eu-repo/semantics/openAccess
Özet
Abstract:Amaç: Bu çalışmada, dizüri şikayeti ile hastanemiz üroloji, kadın hastalıkları ve doğum ve dahiliye polikliniklerine başvuran ve ilk idrar kültürlerinde üreme saptanmayan steril piyürili hastalardan istenen orta akım idrarında, Mycoplasma hominis ve Ureaplasma urealyticum varlığının ve antibiyotik duyarlılıklarının araştırılması amaçlandı. Gereç ve Yöntem: Mycoplasma hominis ve Ureaplasma urealyticum bakterilerini izole etmek ve antibiyotik duyarlılığını belirlemek amacıyla biyokimyasal temelli test kiti olan Mycoplasma System Plus (Liofilchem Diagnostics, İtalya) kullanıldı. Bulgular: Laboratuvara gelen 139u (%61) kadın ve 89u (%39) erkek toplam 228 steril piyürili hasta örneği değerlendirmeye alındı. Hastalar yaşlarına göre gruplara ayrıldı, 79unun (%34.6) 15-35 yaş grubunda, 60ının (%26.3) 36-55 yaş grubunda, 89unun (%39) 56 yaş ve üzerinde olduğu tespit edildi. Üremelerin mevsimlere göre dağılımında; Mycoplasma hominis izolasyon oranı kış aylarında %13.8 (n:22), ilkbahar aylarında %8.8 (n:5) iken Ureaplasma urealyticum izolasyon oranı kış aylarında %17 (n:27), ilkbahar aylarında %17.5 (n:10) olarak bulundu. Yaz aylarında her iki etken de izole edilemedi. İzole edilen bakterilerden Mycoplasma hominise etkinliği en düşük olan antimikrobiyal ajan ofloksasin olarak tespit edilirken; Ureaplasma urealyticum için ise klaritromisinin etkinliği test edilen antimikrobiyaller içinde göreceli olarak düşük bulundu. Sonuç: Çalışmamızda steril piyürisi olan ve rutin kültürlerde üremesi saptanmayan hastalarda etken olarak ürogenital mikoplazma- üreaplazma türlerinin akla getirilmesi gerektiği ve izolasyon, identifikasyon ve duyarlılık testlerine yönelik hemen hemen tüm laboratuvar seviyesinde biyokimyasal tanı kitleri kullanılabileceğini düşünüyoruz.
Abstract:Aim: In this study, we aim to investigate the presence of Mycoplasma hominis and Ureaplasma urealyticum antimicrobial susceptibilities at mid-stream urine samples from outpatients who were admitted to Urology, Obstetrics and Gynecology, and Internal Medicine outpatient clinics with dysuria complaints with sterile pyuria negative in first culture results. Material and Method: To test M. Mycoplasma hominis and Ureaplasma urealyticum isolation and antibiotic susceptibility we have used Mycoplasma System Plus (Liofilchem Diagnostics, Italy) kit, which is based on biochemical reaction interpretation. Results: A total of 228 urine samples, from 139 (61%) women and 89 (39%) men with sterile pyuria, were evaluated in the laboratory. Patients were classified according to their ages, 79 (34.6%) of the patients were in 15-35 age group, 60 (26.3%) of the patients were in 36- 55 age group, and 89 (39%) were above 56. Seasonal distribution of isolates were also assessed. While Mycoplasma hominis isolation rate in winter months was 13.8% (n:22), in spring it was %8.8 (n:5); Ureaplasma urealyticum isolation rate in winter was 17% (n:27) and %17.5 (n:10) in spring. No isolates could be found in the samples from the summer. The least susceptible antimicrobial to Mycoplasma hominis was ofloxacin while clarithromycin was found relatively less effective on Ureaplasma urealyticum among the tested antimicrobials. Conclusion: In our study, we have considered the urogenital Mycoplasma and Ureaplasma species as pathogens in cases with steril pyuria through implementing a biochemical diagnostic kit in all the laboratories for the isolation, identification, and antimicrobial susceptibility tests.
Abstract:Aim: In this study, we aim to investigate the presence of Mycoplasma hominis and Ureaplasma urealyticum antimicrobial susceptibilities at mid-stream urine samples from outpatients who were admitted to Urology, Obstetrics and Gynecology, and Internal Medicine outpatient clinics with dysuria complaints with sterile pyuria negative in first culture results. Material and Method: To test M. Mycoplasma hominis and Ureaplasma urealyticum isolation and antibiotic susceptibility we have used Mycoplasma System Plus (Liofilchem Diagnostics, Italy) kit, which is based on biochemical reaction interpretation. Results: A total of 228 urine samples, from 139 (61%) women and 89 (39%) men with sterile pyuria, were evaluated in the laboratory. Patients were classified according to their ages, 79 (34.6%) of the patients were in 15-35 age group, 60 (26.3%) of the patients were in 36- 55 age group, and 89 (39%) were above 56. Seasonal distribution of isolates were also assessed. While Mycoplasma hominis isolation rate in winter months was 13.8% (n:22), in spring it was %8.8 (n:5); Ureaplasma urealyticum isolation rate in winter was 17% (n:27) and %17.5 (n:10) in spring. No isolates could be found in the samples from the summer. The least susceptible antimicrobial to Mycoplasma hominis was ofloxacin while clarithromycin was found relatively less effective on Ureaplasma urealyticum among the tested antimicrobials. Conclusion: In our study, we have considered the urogenital Mycoplasma and Ureaplasma species as pathogens in cases with steril pyuria through implementing a biochemical diagnostic kit in all the laboratories for the isolation, identification, and antimicrobial susceptibility tests.
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İnönü Üniversitesi Turgut Özal Tıp Merkezi Dergisi
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SELEK B. M,BEKTÖRE B,ATİK T. K,YALÇIN B,BAYLAN O,ÖZYURT M (2014). Investigating the Presence of Mycoplasma Hominis-Ureaplasma Urealyticum and in Vitro Antimicrobial Susceptibilities in Patients With Sterile Pyuria. İnönü Üniversitesi Turgut Özal Tıp Merkezi Dergisi, 21(4), 286 - 290.
