Deep phenotyping of miRNAs in exercise-induced cardiac hypertrophy and fibrosis

Basit Öğe Kaydı
dc.authoridEşrefoğlu, Mukaddes/0000-0003-3380-1480
dc.authoridPala, Mukaddes/0000-0002-0610-0526
dc.authoridKUMAŞ KULUALP, Meltem/0000-0001-6903-3212
dc.authoridAltan, Mehmet/0000-0002-3275-1234;
dc.authorwosidEşrefoğlu, Mukaddes/JWA-4590-2024
dc.authorwosidsönmez, osman fuat/AGT-4063-2022
dc.authorwosidPala, Mukaddes/HKE-7637-2023
dc.authorwosidKUMAŞ KULUALP, Meltem/JFJ-2131-2023
dc.authorwosidAltan, Mehmet/D-7231-2019
dc.authorwosidDinçer, Şensu/HZK-5963-2023
dc.contributor.authorPala, Mukaddes
dc.contributor.authorYilmaz, Senay Gorucu
dc.contributor.authorAltan, Mehmet
dc.contributor.authorSonmez, Osman Fuat
dc.contributor.authorDincer, Sensu
dc.contributor.authorMengi, Murat
dc.contributor.authorKarabulut, Aydin
dc.date.accessioned2024-08-04T20:57:35Z
dc.date.available2024-08-04T20:57:35Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractCardiac hypertrophy (CH) is an adaptational enlargement of the myocardium, in exposure to altered stress conditions or in case of injury which can lead to heart failure and death. MicroRNAs (miRNAs) are non-coding RNAs that play a significant role in modulating gene expression. Here, we aimed to identify new miRNAs effective in an experimental CH model and to find an epigenetic biomarker that could demonstrate therapeutic targets responsible for the pathology of heart tissue and serum. In this study, Sprague-Dawley male rats were divided into the training group (TG, n=9) and the control group (CG, n=6). Systolic and diastolic dimensions of the left ventricle and myocardial wall thickness were measured by echocardiography to assess CH. After the exercise program of the rats, miRNA expression measurements and histological analyses were performed. The 25,000 genes in the rat genome were searched using microarray analysis. A total of 128 miRNAs were selected according to the fold change rates, and nine miRNAs were validated for expression analysis. The terminal deoxynucleotidyl transferase dUTP nick (TUNEL) method was used to detect apoptotic cells. Cell proliferation was evaluated by the proliferative cell nuclear antigen (PCNA) method. Necrosis, bleeding, and intercellular edema were detected in TG. The mean histopathological score was higher in TG (p=0.03). There were rarely positive cells for apoptosis of both groups in cardiomyocytes. In the receiver characteristic curve analysis (ROC), the heart tissue rno-miR-290 had an area under the curve (AUC) of 0.920 with 100% sensitivity and 89.90% specificity (p=0.045), rno-miR-194-5p had AUC of 0.940 with 83.33% sensitivity and 100% specificity (p=0.003), and the serum rno-miR-132-3p AUC was 0.880 with 66.67% sensitivity and 100% specificity (p=0.004) in TG. miR-194-5p was used as a therapeutic target for remodeling the cardiac process. While miR-290 contributes to CH as a negative regulator, miR-132 in serum is effective in the pathological and physiological cardiac remodeling process and is a candidate biomarker.en_US
dc.description.sponsorshipScientific Research Projects Unit of Istanbul University [48783]en_US
dc.description.sponsorshipThis work was supported by the Scientific Research Projects Unit of Istanbul University (Project No.: 48783).en_US
dc.identifier.doi10.1007/s12038-023-00360-4
dc.identifier.issn0250-5991
dc.identifier.issn0973-7138
dc.identifier.issue4en_US
dc.identifier.urihttps://doi.org/10.1007/s12038-023-00360-4
dc.identifier.urihttps://hdl.handle.net/11616/102754
dc.identifier.volume48en_US
dc.identifier.wosWOS:001072400900001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherIndian Acad Sciencesen_US
dc.relation.ispartofJournal of Biosciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiomarkeren_US
dc.subjectcardiac hypertrophyen_US
dc.subjectcirculating miRNAsen_US
dc.subjectgene expressionen_US
dc.subjectmiRNA expressionen_US
dc.subjectswimming trainingen_US
dc.titleDeep phenotyping of miRNAs in exercise-induced cardiac hypertrophy and fibrosisen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu