Thioredoxin is required for deoxyribonucleotide pool maintenance during S phase
| dc.contributor.author | Koç, Ahmet | |
| dc.contributor.author | Mathews, Christopher K. | |
| dc.contributor.author | Wheeler, Linda J. | |
| dc.contributor.author | Gross, Michael K. | |
| dc.contributor.author | Merrill, Gary F. | |
| dc.date.accessioned | 2017-06-19T08:07:54Z | |
| dc.date.available | 2017-06-19T08:07:54Z | |
| dc.date.issued | 2006 | |
| dc.department | İnönü Üniversitesi | en_US |
| dc.description | J Biol Chem | en_US |
| dc.description.abstract | Thioredoxin was initially identified by its ability to serve as an electron donor for ribonucleotide reductase in vitro. Whether it serves a similar function in vivo is unclear. In Saccharomyces cerevisiae, it was previously shown that trx1 trx2 mutants lacking the two genes for cytosolic thioredoxin have a slower growth rate because of a longer S phase, but the basis for S phase elongation was not identified. The hypothesis that S phase protraction was due to inefficient dNTP synthesis was investigated by measuring dNTP levels in asynchronous and synchronized wild-type and trx1 trx2 yeast. In contrast to wild-type cells, trx1 trx2 cells were unable to accumulate or maintain high levels of dNTPs when -factor- or cdc15-arrested cells were allowed to reenter the cell cycle. At 80 min after release, when the fraction of cells in S phase was maximal, the dNTP pools in trx1 trx2 cells were 60% that of wild-type cells. The data suggest that, in the absence of thioredoxin, cells cannot support the high rate of dNTP synthesis required for efficient DNA synthesis during S phase. The results constitute in vivo evidence for thioredoxin being a physiologically relevant electron donor for ribonucleotide reductase during DNA precursor synthesis. | en_US |
| dc.identifier.citation | KOÇ, A., Chris, M., Linda, W., Micheal, G., & Gary, M. (2006). Thioredoxin is required for deoxyribonucleotide pool maintenance during S phase . J Biol Chem, (281(22)), 15058–0. | en_US |
| dc.identifier.endpage | 0 | en_US |
| dc.identifier.issue | 22 | en_US |
| dc.identifier.startpage | 15058 | en_US |
| dc.identifier.uri | http://www.jbc.org/content/281/22/15058.full.pdf | |
| dc.identifier.uri | https://hdl.handle.net/11616/7103 | |
| dc.identifier.volume | 281 | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | J Biol Chem | en_US |
| dc.relation.ispartof | J Biol Chem | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.title | Thioredoxin is required for deoxyribonucleotide pool maintenance during S phase | en_US |
| dc.type | Article | en_US |
