ASSESSMENT OF PHYTOTOXIC AND GENOTOXIC EFFECTS OF ANATASE TiO2 NANOPARTICLES ON MAIZE CULTIVAR BY USING RAPD ANALYSIS
Küçük Resim Yok
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications (P S P)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, early phytotoxic and genotoxic effects of chemically synthesized nano-TiO2 (anatase) were investigated in seedlings of maize (Zea mays cv. Hido). Control (Hoagland) and NaCl (300 mM) groups were administered with nano-TiO2 (0.1%, 0.2% and 0.3%). Seed germination and seedling growth parameters of maize cultivar were markedly inhibited by salt stress. Nano-TiO2, without depending on concentration, was found to be ineffective on germination percentage in all administrations. On the contrary, nano-TiO2 caused significant increases in root-stem length and fresh-dry weights especially in NaCl+nano-TiO2 administrations. In salt administered samples, 0.3% nano-TiO2 increased root length 1.4 fold, stem length 4.8 fold, and fresh weight 1.2 fold. Genotoxic properties of nano-TiO2 in seedlings of maize were evaluated by using randomly amplified polymorphic DNA (RAPD-PCR). In RAPD analysis, 20 RAPD primers were found to produce unique polymorphic band profiles at different concentrations of nano-TiO2 maize seedlings. Genomic template stability (GTS), a qualitative measurement of changes in RAPD patterns of genomic DNA, decreased depending on increasing nano-TiO2, NaCl, and NaCl+nano-TiO2 concentrations. Therefore, it could be concluded that nano-TiO2 of appropriate dose can be used to ameliorate negative effects of salt stress by increasing growth potential of maize.
Açıklama
Anahtar Kelimeler
RAPD, genotoxic effects, Zea mays, nano-TiO2, genomic template stability, germination seedling, growth parameters
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
27
Sayı
1
