The Development of Ternary Nanoplexes for Efficient Small Interfering RNA Delivery

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dc.authoridŞALVA, EMINE/0000-0002-1159-5850
dc.authoridSalva, Emine/0000-0002-1159-5850
dc.authorwosid/AAD-1704-2020
dc.authorwosidŞALVA, EMINE/CAH-3062-2022
dc.authorwosidSalva, Emine/ABI-2766-2020
dc.contributor.authorSalva, Emine
dc.contributor.authorTuran, Suna Ozbas
dc.contributor.authorAkbuga, Julide
dc.date.accessioned2024-08-04T21:01:22Z
dc.date.available2024-08-04T21:01:22Z
dc.date.issued2013
dc.departmentİnönü Üniversitesien_US
dc.description.abstractTargeted posttranscriptional gene silencing by RNA interference (RNAi) has garnered considerable interest as an attractive new class of drugs for several diseases, such as cancer. Chitosan and protamine are commonly used as a vehicle to deliver and protect small interfering RNA (siRNA), but the strong interaction still remains to be modulated for efficient siRNA uptake and silencing. Therefore, in this study, ternary nanoplexes containing chitosan and protamine were designed to substantially enhance the siRNA efficiency. Binary and ternary nanoplexes were prepared at different the ratios of moles of the amine groups of cationic polymers to those of the phosphate ones of siRNA (N/P) ratios and characterized in terms of size, zeta potential, morphology and serum stability. The silencing efficiencies and cytotoxicities of prepared nanoplexes were evaluated by enzyme-linked immunosorbent assay (ELISA) (for human vascular endothelial growth factor; hVEGF) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, respectively. The mean diameter of ternary nanoplexes ranged from 151 to 282nm, depending on the weight ratio between polymers and siRNA. The gene silencing effect after transfection with ternary nanoplexes (chitosan/siRNA/protamine 83%) was significantly higher than that with binary nanoplexes (chitosan/siRNA 71% and protamine/siRNA 74%). Ternary nanoplexes showed the highest cellular uptake ability when compared with binary nanoplexes. Ternary nanoplexes did not induce substantial cytotoxicity. Serum stability and the lack of cytotoxicity of the nanoplexes provided advantages over other gene silencing studies. These results suggest ternary nanoplexes have the potential to be an effective siRNA carrier to study the gene silencing effect.en_US
dc.description.sponsorshipMarmara University Scientific Research Projects Association [SAG-D-140312-0043]en_US
dc.description.sponsorshipThis study was supported by Marmara University Scientific Research Projects Association (SAG-D-140312-0043).en_US
dc.identifier.endpage1914en_US
dc.identifier.issn0918-6158
dc.identifier.issue12en_US
dc.identifier.pmid24432377en_US
dc.identifier.startpage1907en_US
dc.identifier.urihttps://hdl.handle.net/11616/104315
dc.identifier.volume36en_US
dc.identifier.wosWOS:000327573800005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPharmaceutical Soc Japanen_US
dc.relation.ispartofBiological & Pharmaceutical Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectsmall interfering RNAen_US
dc.subjectprotamineen_US
dc.subjectchitosanen_US
dc.subjectternary nanoplexen_US
dc.subjectvascular endothelial growth factoren_US
dc.titleThe Development of Ternary Nanoplexes for Efficient Small Interfering RNA Deliveryen_US
dc.typeArticleen_US

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