PREPARATION AND OPTIMIZATION OF CONDUCTING POLYMER (POLYPYRROLE) AS A GLUCOSE SENSOR
Küçük Resim Yok
Tarih
1995
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technological Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An amperometric enzyme electrode for glucose determination was prepared by anodic polymerization of pyrrole and concomitant incorporation of glucose oxidase on Pt substrates in A KCl solution at a potential of 0.8 V vs. Ag/AgCl. The amperometric response to glucose was measured at a potential of 0.7 V in order to oxidize the hydrogen peroxide generated. Effects of various parameters (e.g., film thickness, concentrations of monomer, supporting electrolyte, and enzyme) on the electrode preparation and the effects of buffer concentration, temperature, and pH, on the chronoamperometric response of the prepared enzyme electrode were systematically investigated and the optimum values for each parameter were determined. If was found that a biosensor, with a response time of less than 5 s, can be easily prepared in ca. 5 min. Activation energy of the enzymatic reaction was calculated as 13 kJ/mol and the linearity of the enzyme electrode response ranged from 2.0 to 15 mM of glucose. The specificity of the enzyme electrode was tested for ascorbic, succinic and maleic acids, and sucrose, and no discernible signal was detected above the background current. Moreover, the response of the electrode was found to be stable for about one month.
Açıklama
Anahtar Kelimeler
GLUCOSE, GLUCOSE OXIDASE, POLYPYRROLE, ENZYME ELECTRODE, AMPEROMETRIC BIOSENSOR
Kaynak
Turkish Journal of Chemistry
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
19
Sayı
3
