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  • Küçük Resim Yok
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    Ratlarda deneysel global serebral iskemi ve reperfüzyon modelinde Cinnamon Bark Oil'in beyin dokularında antioksidan ve oksidan etkilerinin araştırılması
    (İnönü Üniversitesi, 2021) Aba, Melike; Kablan, Yüksel
    Amaç: Cinnamomum verum tarçının kabuğunda bulan ve bir esansiyel yağ olan Cinnamon Bark Oil'in (CBO) geniş bir aralığa yayılan farmakolojik etkiler gösterdiği bilinmektedir. CBO'nun serebral iskemi reperfüzyon (I/R) hasarında antioksidan, anti-inflamatuar, nöronal apoptozun düzenlenmesi ve nöroprotektif etkileri üzerine yapılmış çok az çalışma bulunmaktadır. Bu çalışmada CBO'nun bu etkilerini I/R hasarı oluşturulmuş rat modelinde araştırmayı amaçladık. Gereç ve Yöntem: Çalışmada 44 adet Wistar albino cinsi dişi rat 4 gruba ayrıldı; sham grubuna (n:8) CBO sham operasyon sonrası 1, 24, 48 ve 72.saatte, sadece I/R oluşturulan grup (n:12), proflaksi grubuna (n:12) CBO cerrahi öncesi 72, 48, 24 ve cerrahiden 1 saat önce en son doz, tedavi grubuna (n:12) CBO cerrahi girişimden 1, 24, 48, 72 saat sonra verildi. I/R modeli oluşturmak için iki yanlı karotis kommunis arterler 15 dakika klemplenerek iskemi oluşturuldu ve sonra açılarak reperfüzyon sağlandı. Ratlarda 72 saat reperfüzyon sağlandıktan sonra sakrifikasyon işlemi uyglandı. CBO, 100 mg/kg dozunda, oral yolla ve günde bir defa verildi. Ratların beyin dokusunda histopatolojik incelemelerle birlikte süperoksid dismutaz (SOD), total glutatyon(tGSH), total antioksidan kapasite (TAK), total oksidan kapasite (TOK), malondialdehit (MDA), myeloperoksidaz(MPO), İnterlökin-1(IL-1) ve TNF-? düzeyleri bakıldı. Bulgular: Hem proflaksi hemde tedavi edilen grupta immünhistokimyasal boyamada (kaspaz-3, Bax) kortikal ve hipokampal nöronlarda dejenere nöron sayısında anlamlı azalma olduğu görüldü. Ayrıca ratların beyin dokusundaki I/R oksidatif ve inflamatuar hasarda, proflaksi grubunda SOD aktivitesi, hem proflaksi hem tedavi grubunda TAC düzeyi artmış buna karşılık MDA ve TNF düzeyleri her iki grubta anlamlı azalmıştı. Sonuçlar: Bu bulgular CBO'nun olası antioksidan, anti-inflamatuar ve antiapoptotik etkileriyle hem proflakside hemde tedavide I/R hasarından koruyucu olabileceğini göstermektedir. Anahtar Kelimeler: Serebral iskemi, reperfüzyon hasarı, cinnamon bark oil, rat modeli
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    The Role of the Low-Density Lipoprotein/High-Density Lipoprotein Cholesterol Ratio as an Atherogenic Risk Factor in Young Adults with Ischemic Stroke: A Case-Control Study
    (Mdpi, 2023) Ciplak, Sibel; Adiguzel, Ahmet; Deniz, Yusuf Ziya; Aba, Melike; Ozturk, Unal
    Dyslipidemia is a major atherogenic risk factor for ischemic stroke. Stroke patients tend to have high levels of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) and low levels of high-density lipoprotein cholesterol (HDL-C). Therefore, it is noteworthy that there has been an increase in ischemic stroke cases in young and elderly individuals in recent years. This study investigated the TC/HDL-C ratio and the LDL-C/HDL-C ratio, which may be more specific and common lipid parameters in young patients with ischemic stroke. This study aimed to demonstrate the sensitivity and specificity of TC/HDL-C and LDL-C/HDL-C ratios as atherogenic markers for young adult ischemic strokes. This trial was conducted as a retrospective case-control study. A total of 123 patients (patient group) and 86 healthy individuals (control group) aged 18-50 years were randomly selected from four different hospitals. Lipid parameters and TC/HDL-C and LDL-C/HDL-C ratios were compared between these two groups. The mean age was 38.8 +/- 7.3 years in patients and 37.7 +/- 9 years in controls (p > 005). The HDL-C levels were 39.1 +/- 10.8 mg/dL in patients and 48.4 +/- 13.8 mg/dL in controls (p < 0.001). LDL-C/HDL-C ratios were 3.23 +/- 1.74 and 2.38 +/- 0.87, and TC/HDL-C ratios were 5.24 +/- 2.31 and 4.10 +/- 1.25 in the patient and control groups, respectively (p < 0.001). The LDL-C/HDL-C and TC/HDL-C cutoff values in ROC analyses were 2.61 and 4.40 respectively; the AUCs (95% CI) were determined to be 0.680 (0.608-0.753) and 0.683 (0.610-0.755) (p < 0.001), respectively. An increased risk of stroke was observed in those with a high LDL-C/HDL-C ratio (OR = 1.827; 95% CI = 1.341-2.488; p < 0.001). Our study obtained similar results when we compared the mean TC and LDL-C levels between the two groups. However, considering the TC/HDL-C and LDL-C/HDL-C ratios, it is noteworthy that there was a significant difference between the patient and control groups.
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    The effect of cinnamon bark oil in bilateral common carotid artery occlusion-induced cerebral ıischemia model in rats
    (Nature Portfolio, 2025) Aba, Melike; Kablan, Yuksel; Ozhan, Onural; Karaca, Elif; Ulu, Ahmet; Ates, Burhan; Vardi, Nigar
    This study aimed to evaluate the protective and therapeutic effects of Cinnamon Bark Oil (CBO) on cerebral ischemia/reperfusion (I/R) injury in rats. The focus was on its potential antioxidant, anti-inflammatory, and antiapoptotic properties. Forty-four female Wistar albino rats were divided into four groups: Sham + CBO group, I/R + Vehicle group, prophylactic (CBO + I/R) group and therapeutic (I/R + CBO) group. Bilateral common carotid artery occlusion was performed to induce ischemia, followed by reperfusion for 72 h. CBO (100 mg/kg) was administered orally. Biochemical, histopathological, and immunohistochemical analyses were conducted, measuring oxidative stress markers, inflammatory cytokines, and neuronal degeneration in the cerebral cortex and hippocampus. Histopathological analysis revealed that CBO significantly reduced neuronal degeneration in both the prophylactic and therapeutic groups. Biochemically, CBO increased superoxide dismutase (SOD) and total antioxidant capacity (TAC), while decreasing malondialdehyde (MDA) and tumor necrosis factor-alpha (TNF-alpha) levels. Immunohistochemical staining showed decreased apoptotic markers (caspase-3, Bax) in the prophylactic and therapeutic groups. CBO demonstrated neuroprotective effects in cerebral I/R injury through its antioxidant, anti-inflammatory, and antiapoptotic properties. These findings suggest its potential as a complementary therapeutic agent for ischemic brain injuries.