Yazar "Akyuz, Mustafa" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    Effects of drugs commonly used in Sars-CoV-2 infection on renal tissue in rats
    (2023) Yıldız, Azibe; Ozhan, Onural; Vardi, Nigar; Akyuz, Mustafa; Bakar, Busra; Ulu, Ahmet; Taslıdere, Elif
    Aim: In December 2019, the coronavirus disease was caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Some drugs were repurposed for this disease treatment. Hydroxychloroquine (HCQ), favipiravir (FAV), molnupiravir (MOL), and dexamethasone (DEX) were widely used for the treatment of the disease. To increase the success of the treatment of coronavirus disease, there was used some of these drugs in combination. On the other hand, limited studies report these drugs’ side effects. Therefore, this study was designed to evaluate the possible effects of these drugs and their combinations on the kidney tissues of rats without viral load. Materials and Methods: Male Wistar albino rats were divided into control, HCQ, FAV, HCQ+FAV, HCQ+FAV+DEX, MOL, and MOL+DEX groups. At the end of the experiment, the serum kidney tissue samples were taken. Serum samples were analyzed for urea and creatinine. Kidney tissue samples were assessed as histopathological and immunohistochemical for heat shock protein 60 (HSP60), caspase-3, and receptor-interacting protein kinase-3 (RIPK3). Results: Urea and creatinine levels were within the normal range in all groups. Histopathologically, all drugs and their combinations caused tubular degeneration. On the other hand, histopathological alterations were more prominent in the HCQ group. The oxidative stress marker HSP60 was significantly increased in FAV, MOL, and MOL+DEX groups, while it was similar to the control group in the HCQ groups. Apoptosis marker caspase-3 expression was found to be prominently higher in other drug groups except the FAV group. Expression of RIPK3, a marker of necroptosis, was significantly increased in all drug groups. Conclusion: Taken together, the data of our study show that the administration of all drugs alone and in combination may cause structural damage to the kidney. Furthermore, our results indicate that HCQ can exhibit more damaging effects compared to other drugs.
  • Küçük Resim Yok
    Öğe
    Efficacy of Modified Talc Powder in Experimental Rat Model of Pleurodesis
    (Mdpi, 2026) Kilic, Murat; Ozhan, Onural; Yildiz, Azibe; Koytepe, Suleyman; Akyuz, Mustafa; Turkoz, Yusuf; Gokturk, Nurcan
    Background: Pleurodesis is a treatment method that aims to create permanent adhesion between the pleural layers to prevent recurrent fluid or air accumulation in the pleural cavity. Talc, one of the most commonly preferred agents in this procedure, is widely used in clinical practice. In this study, a new talc formulation with a modified surface to impart antibacterial and analgesic properties was experimentally evaluated for the first time. The main objective of the study was to comparatively assess the inflammatory and fibrotic responses following standard talc and modified talc applications. Methods: Thirty-six 12-week-old female Wistar albino rats were simply randomly divided into three different groups: control (n = 12), standard talc (n = 12), and modified talc (n = 12). Under anesthesia, 1 mL of physiological saline containing 17 mg of talc was injected intrapleurally into the right hemithorax. The presence of pneumothorax after the procedure was assessed by chest radiography. After a 12-day follow-up period, the animals were euthanized. Bronchoalveolar lavage (BAL) fluid samples, blood samples, and lung and pleural tissue samples were collected for biochemical, histopathological, and immunohistochemical analyses. Results: Modified talc application resulted in a significant increase in both visceral and parietal pleural thickness (p < 0.05). Granulation tissue formation and collagen deposition were significantly higher in the modified talc group. In addition, TGF-beta expression and CD68-positive macrophage count increased significantly in the modified talc group (p < 0.05). Inflammatory changes in the lung parenchyma were limited and not statistically significant. Conclusions: The modified talc formulation enriched with lidocaine and antibacterial agents produced a stronger inflammatory and fibrotic response compared to standard talc. These findings indicate that modified talc may increase the effectiveness of pleurodesis. Furthermore, the absence of significant lung parenchymal damage suggests that this treatment is locally effective and feasible. However, further long-term and advanced studies are needed to translate these results into clinical use.
  • Küçük Resim Yok
    Öğe
    Vitamin D mitigates cisplatin-mediated acute kidney injury through modulation of NRF2/HO-1 and autophagy signaling pathways
    (Churchill Livingstone, 2026) Yildiz, Azibe; Tanbek, Kevser; Cuglan, Songul; Koca, Gokce; Akyuz, Mustafa; Vardi, Nigar
    This study investigated the effects of vitamin D (Vit D) pretreatment on renal oxidative stress in cisplatin (CP)induced acute kidney injury (AKI). Twenty-one six-month-old female Wistar albino rats (277.3 +/- 11.8 g) were randomly divided into three groups (n = 7): Control, CP, and Vit D+CP. The control group received intraperitoneal 0.9% NaCl for 7 days. The CP group received a single intraperitoneal dose of CP (7 mg/kg) on day 1 of the experiment. The Vit D+CP group received a single intraperitoneal dose of CP (7 mg/kg) on day 1, followed by daily intraperitoneal Vit D (1000 IU/mL) for 7 consecutive days. The oxidative stress index (OSI) of kidney tissue was calculated based on total antioxidant status (TAS) and total oxidant status (TOS) measurements. Furthermore, tissue samples were assessed histologically and immunohistochemically for NRF2, HO-1, BECN1, LC3B, and vimentin proteins. CP, which significantly increased the OSI values and the immunoreactivity of NRF2, HO1, BECN1, LC3B, and vimentin, caused moderate-to-severe tubular damage histopathologically (P < 0.05). By contrast, the OSI was lower in the Vit D+CP group (P < 0.05). Tubular damage was significantly reduced in this group, and NRF2, HO-1, BECN1, LC3B, and vimentin immunoreactivity were also remarkably lower compared with the CP group (P < 0.05). In conclusion, the findings of the present study suggest that Vit D may exert a protective effect against CP-induced AKI. Vit D supplementation during CP chemotherapy may potentially mitigate renal adverse effects; however, further experimental and clinical studies are required to validate these findings.