Vitamin D mitigates cisplatin-mediated acute kidney injury through modulation of NRF2/HO-1 and autophagy signaling pathways
Küçük Resim Yok
Tarih
2026
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Churchill Livingstone
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study investigated the effects of vitamin D (Vit D) pretreatment on renal oxidative stress in cisplatin (CP)induced acute kidney injury (AKI). Twenty-one six-month-old female Wistar albino rats (277.3 +/- 11.8 g) were randomly divided into three groups (n = 7): Control, CP, and Vit D+CP. The control group received intraperitoneal 0.9% NaCl for 7 days. The CP group received a single intraperitoneal dose of CP (7 mg/kg) on day 1 of the experiment. The Vit D+CP group received a single intraperitoneal dose of CP (7 mg/kg) on day 1, followed by daily intraperitoneal Vit D (1000 IU/mL) for 7 consecutive days. The oxidative stress index (OSI) of kidney tissue was calculated based on total antioxidant status (TAS) and total oxidant status (TOS) measurements. Furthermore, tissue samples were assessed histologically and immunohistochemically for NRF2, HO-1, BECN1, LC3B, and vimentin proteins. CP, which significantly increased the OSI values and the immunoreactivity of NRF2, HO1, BECN1, LC3B, and vimentin, caused moderate-to-severe tubular damage histopathologically (P < 0.05). By contrast, the OSI was lower in the Vit D+CP group (P < 0.05). Tubular damage was significantly reduced in this group, and NRF2, HO-1, BECN1, LC3B, and vimentin immunoreactivity were also remarkably lower compared with the CP group (P < 0.05). In conclusion, the findings of the present study suggest that Vit D may exert a protective effect against CP-induced AKI. Vit D supplementation during CP chemotherapy may potentially mitigate renal adverse effects; however, further experimental and clinical studies are required to validate these findings.
Açıklama
Anahtar Kelimeler
Acute kidney injury, Autophahgy, Cisplatin, NRF2/HO-1, Oxidative status, Vitamin D
Kaynak
Tissue & Cell
WoS Q Değeri
Q1
Scopus Q Değeri
Q3
Cilt
101
