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Öğe Biostimulatory Effects of Diode Laser and Ozone on Wound Healing in Rats(Galenos Yayincilik, 2018) Alan, Hilal; Guler, Cigdem; Yolcu, Umit; Koparal, Mahmut; Cakir, Ebru; Demir, PinarObjective: Diode laser and ozone can be used to healing of skin wounds. The aim of this study is to evaluate the biostimulatory effects of diode laser and ozone on the healing of sutured skin wounds in rats. Materials and Methods: In this study, 21 adult males Wistar albino rats were used. Three 1-cm-longs, full-thickness incisions were applied to the back of each animal and closed with sutures. Diode laser treatment, irrigation with 0.9% saline solution and ozone therapy was applied to the wounds through 7 days. Animals were euthanized on the 3rd, 7th, and 14th days after treatment, and all wounds were surgically removed for histopathological evaluation. Results: According to histological results, a statistically significant difference was found among the three treatment methods in acute inflammatory reactions on 3rd day (p<0.05). Ozone treatment reduced the intensity of acute inflammatory reactions by the 3rd day (p<0.05). In addition, a statistically significant difference was found between the follow-up periods in all treatment groups for fibrosis (p<0.01). Conclusion: It was found that ozone treatment was more effective when compared to the other treatments for reducing the intensity of acute inflammatory reactions on the healing of sutured skin wounds.Öğe Bir hastada eş zamanlı keratokistik odontojenik tümör ve radiküler kist oluşumu: Olgu sunumu(Mersin Üniversitesi Sağlık Bilimleri Dergisi, 2018) Alan, Hilal; Özcan Küçük, Ayşe; Yolcu, Ümit; Aydın, Nasuhi EnginÖz: Odontojenik kistler yaygın görülmesine rağmen, aynı hastada iki farklı ve eş zamanlı odontojenik lezyonun oluşumu oldukça nadirdir. Daha önce, keratokisitk odontojenik tümörün adenomatoid odontojenik tümör, ameloblastoma, dev hücreli granulom, travmatik kemik kisti, palatal pleomorfik adenoma gibi çeşitli odontojenik ve non-odontojenik lezyonlarla beraber eş zamanlı oluşumu tanımlanmıştır. Ayrıca radiküler kist ile beraber mukoepidermoid karsinomu bulunan bir olgu da rapor edilmiştir. Literatürde, eş zamanlı keratokistik odontojenik tümör ve radiküler kistin eş zamanlı olarak ortaya çıktığı benzer bir vaka daha önce bildirilmemiştir. Bu olgu sunumunda, 26 yaşında bir erkeğin mandibula posterior bölgesinde radiküler kist ve keratokistik odontojenik tümörün eş zamanlı oluşumu gibi nadir bir durum sunulmaktadır. Başlık (İngilizce): Simultaneous occurrence of keratocystic odontogenic tumor and radicular cyst in a patient: A case report Öz (İngilizce): Although odontogenic cysts are commonly seen, appearence of two different and simultaneous odontogenic lesions in the same patient is extremely rare. Previously, simultaneous occurrence of keratocystc odontogenic tumor and various odontogenic and non-odontogenic lesions such as adenomatoid odontogenic tumor, ameloblastoma, giant cell granuloma, traumatic bone cyst, and palatal pleomorphic adenoma have been described. A case with co-occurence radicular cyst and mucoepidermoid carcinoma has also been reported. In the literature, a similar case of the simultaneous occurrence of keratocystic odontogenic tumor and radicular cyst has not been reported previously. In this case report, a rare case of the simultaneous occurrence of keratocystic odontogenic tumor and radicular cyst in the posterior region of the mandible of a 26-year-old male is presented.Öğe Bone regeneration by low-level laser therapy and low-intensity pulsed ultrasound therapy in the rabbit calvarium(Pergamon-Elsevier Science Ltd, 2016) Acar, Ahmet Huseyin; Yolcu, Umit; Altindis, Sedat; Gul, Mehmet; Alan, Hilal; Malkoc, SiddikObjective: We evaluated the efficacy of low-level laser therapy (LLLT) and low-intensity pulsed ultrasound (LIPUS), alone and in combination, in triggering new bone formation. Study design: Sixteen New Zealand white rabbits were given two calvarial defects by using a 6-mm trephine bur, then divided into four treatment groups: control, LLLT, LIPUS, and LLLT + LIPUS. The LLLT and LIPUS groups were treated three times a week for two weeks. The LLLT + LIPUS group received each treatment on the same day, 12 h apart, three days a week for two weeks. The animals were sacrificed after three weeks. Results: LLLT and LIPUS, alone and in combination, enhanced new bone formation in comparison to the untreated controls after three weeks (P < 0.05); the combined therapy did not produce an additive effect. Conclusions: Our results demonstrate the efficacy of LLLT or LIPUS in triggering bone regeneration. Therapeutic dose and duration requires further study. (C) 2015 Elsevier Ltd. All rights reserved.Öğe Büyük Agresif Bir Santral Dev Hücreli Granuloma Birlikte Tedavisi (Olgu Sunumu)(2017) Koparal, Mahmut; Alan, Hilal; Ağaçayak, Serkan; Erdoğdu, Halil; Gülsün, BelginÖz: Santral Dev Hücreli tümörler; çene tümörleri içinde kadınlarda ve mandibulada sık görülürler ve yaşamın ikinci dekatında daha sıktırlar. Tedavisinde lokal küretaj, parsiyel rezeksiyon ve total rezeksiyon uygulanabilir. Bu vaka raporunda 32 yaşında kadın hasta anterior mandibulada ağrı şikayetiyle kliniğimize başvurdu. Fiziksel muayenede servikal lenfadenopati saptanmadı. İntraorol muayenede ülserasyon saptanmadı fakat vestibül bölgede hassasiyet mevcuttu. Bununla beraber dişlerde ve dudakta his kaybı yoktu. Bu vaka raporunda 32 yaşında kadın hastada mandibula basisinde yaygın kemik kaybına neden olan santral dev hücreli tümörün parsiyel rezeksiyon ile tedavisi sunuldu.Öğe Clinical and radiographic features of odontogenic keratocysts and evaluation of recurrence rates after treatment: A retrospective study(2019) Ege, Bilal; Demirkol, Mehmet; Yalçın, Mustafa; Alan, HilalAbstract: Aim: In this retrospective study, it was aimed to evaluate the clinical and pathological features of cases diagnosed with OKC and therecurrence status after treatment options.Material and Methods: 41 patients who were treated with the diagnosis of OKC between December 2015-June 2018 and whose preandpost-treatment follow-up data could be reached were included in the study. As the variables examined, age and gender of thepatients, localization of cyst and radiographic features, histopathological findings, treatment methods and recurrence status wereinvestigated retrospectively.Results: Of 41 patients with a mean age of 36.68±17.89 (age range 13 to 78) years, 24 (58.5%) were males and 17 (41.5%) werefemales. It was determined that OKCs were observed most frequently in the third decades (21.9%) followed by the second andfourth decades (19.5%). The incidence of OKC was determined to be higher in the mandible (80.5%) than in the maxilla (19.5%).The recurrence was observed in 7 of 41 cases (17.1%) after the treatment. The recurrence was detected particularly in mandibularposterior and larger lesions. The fact that five of the seven recurrent cases were parakeratotic and likewise five cases were treatedwith enucleation was noteworthy.Conclusions: The treatment and prognosis of OKC should be determined according to the condition of the case and the treatmentprotocol should be managed accordingly.Öğe Comparison of the Anesthetic Efficiency of Lidocaine and Tramadol Hydrochloride in Orthodontic Extractions: A Split-Mouth, Prospective, Randomized, Double-Blind Study(W B Saunders Co-Elsevier Inc, 2020) Ege, Bilal; Ege, Miray; Koparal, Mahmut; Alan, HilalPurpose: The aim of the present randomized controlled split-mouth clinical study was to evaluate and compare the clinical anesthetic efficacy of lidocaine and tramadol hydrochloride in orthodontic extractions. Materials and Methods: A total of 32 systemically healthy patients requiring bilaterally maxillary first premolar extractions for orthodontic reasons were included. Each patient received 1.8 mL of lidocaine (36 mg lidocaine HCI and 0.0225 mg epinephrine) on 1 side and 1.8 mL tramadol (50 mg tramadol HCl and 0.0225 mg epinephrine diluted to 1.8 mL by distilled water) on the other side. The anesthetic solutions were infiltrated into the buccal vestibule (local infiltration) based on a computer-generated list. In each patient, the time of anesthetic onset and finish, anesthetic activity, duration of postoperative analgesia, additional analgesic medication, wound healing, possible side effects, and satisfaction levels were recorded intraoperatively and postoperatively for both sides. Results: Although no relevant differences were found between the solutions for anesthetic onset, lidocaine was significantly more effective statistically for the total anesthesia duration. Comparing the anesthetic activity at 5 minutes before extraction, we found that tramadol was significantly more effective statistically compared with lidocaine. Similarly, tramadol was significantly more effective statistically than lidocaine for satisfaction level and wound healing. Moreover, compared with tramadol, in terms of postoperative pain, the visual analog scale scores with lidocaine were significantly higher at statistically 7, 8, 9, and 10 hours during the first 12 hours. In general, the lidocaine values were dramatically higher than were the tramadol values. Conclusions: The results of the present study suggest that using tramadol combined with epinephrine can be an alternative local anesthetic for maxillary first premolar tooth extractions in oral-maxillofacial surgery. (C) 2019 American Association of Oral and Maxillofacial SurgeonsÖğe Comparison of the Effects of Low-Level Laser Therapy and Ozone Therapy on Bone Healing(JOURNAL OF CRANIOFACIAL SURGERY, 2015) Alan, Hilal; Vardi, Nigar; Ozgur, Cem; Huseyin, Ahmet; Yolcu, Umit; Dogan, Derya OzdemirThis study aims to compare the effect of low-level laser therapy (LLLT) and ozone therapy on the bone healing. Thirty-six adult male Wistar albino rats were used for this study. Monocortical defects were shaped in right femur of all rats. Defects were filled with nano-hydroxyapatite graft. The animals were divided into 3 groups and each group was than divided into 2 subgroups. Then, LLLT with a diode laser was applied to the first group (G1), ozone therapy was applied to the second group (G2), and no treatment was applied to the third group as a control group (G3). Animals were sacrificed after 4th and 8th weeks and the sections were examined to evaluate the density of the inflammation, the formation of connective tissue, the osteogenic potential, and osteocalcin activity. As a result, there were no significant differences among the groups of 4 weeks in terms of new bone formation. In the immunohistochemical assessment, the number of osteocalcin-positive cells was higher in the laser group compared to the other group of 4 weeks; this difference was statistically significant in the LLLT and ozone groups (P < 0.05). Histomorphometric assessment showed that the new bone areas were higher in the LLLT and ozone groups; furthermore, there was a statistically significant difference in the LLLT in comparison with the control group at 8th week (P < 0.05). At the same time immunohistochemical assessment showed that osteocalcin-positive cells were considerably higher in G2 than G1 at 8th week (P < 0.05). The findings of this study may be the result of differences in the number of treatment sessions. Further studies are therefore needed to determine the optimal treatment modality.Öğe Comparison of the Effects of Low-Level Laser Therapy and Ozone Therapy on Bone Healing(Lippincott Williams & Wilkins, 2015) Alan, Hilal; Vardi, Nigar; Ozgur, Cem; Huseyin, Ahmet; Yolcu, Umit; Dogan, Derya OzdemirThis study aims to compare the effect of low-level laser therapy (LLLT) and ozone therapy on the bone healing. Thirty-six adult male Wistar albino rats were used for this study. Monocortical defects were shaped in right femur of all rats. Defects were filled with nano-hydroxyapatite graft. The animals were divided into 3 groups and each group was than divided into 2 subgroups. Then, LLLT with a diode laser was applied to the first group (G1), ozone therapy was applied to the second group (G2), and no treatment was applied to the third group as a control group (G3). Animals were sacrificed after 4th and 8th weeks and the sections were examined to evaluate the density of the inflammation, the formation of connective tissue, the osteogenic potential, and osteocalcin activity. As a result, there were no significant differences among the groups of 4 weeks in terms of new bone formation. In the immunohistochemical assessment, the number of osteocalcin-positive cells was higher in the laser group compared to the other group of 4 weeks; this difference was statistically significant in the LLLT and ozone groups (P < 0.05). Histomorphometric assessment showed that the new bone areas were higher in the LLLT and ozone groups; furthermore, there was a statistically significant difference in the LLLT in comparison with the control group at 8th week (P < 0.05). At the same time immunohistochemical assessment showed that osteocalcin-positive cells were considerably higher in G2 than G1 at 8th week (P < 0.05). The findings of this study may be the result of differences in the number of treatment sessions. Further studies are therefore needed to determine the optimal treatment modality.Öğe Cytotoxicity Evaluation of Bioresorbable Fixation Screws on Human Gingival Fibroblasts and Mouse Osteoblasts by Real-Time Cell Analysis(W B Saunders Co-Elsevier Inc, 2015) Yolcu, Umit; Alan, Hilal; Malkoc, Siddik; Bozkurt, Serife Buket; Hakki, Sema SezginPurpose: To evaluate the effects of bioresorbable fixation screws (BFSs) on human gingival fibroblast (HGF) and mouse osteoblast (MC3T3-E1) cell viability. Materials and Methods: The KLS Martin SonicPins Rx, Synthes RapidSorb Cortex Screws, and Inion CPS Bioabsorbable Fixation System each were incubated in Dulbecco's Modified Eagle Medium for 72 hours according to ISO 10993-5 standards. A real-time cell analyzer was used to evaluate cell survival. After seeding 200-mu L cell suspensions in the wells of an E-plate View 96, HGF and MC3T3-E1 cells were treated with the bioactive components released by the bioresorbable materials and monitored every 15 minutes for 96 hours. Statistical significance was determined using 1-way analysis of variance and Tukey-Kramer tests. Results: There were significant differences in the HGF responses to the untreated control conditions and the Synthes (P < .01), Inion (P < .05), and KLS Martin (P < .05) treatments over 48 hours. The Synthes (P < .01) and Inion (P < .01) treatments produced lower HGF cell index values than the untreated control at 72 hours, whereas the KLS Martin treatment did not. When left to elute for 96 hours, there were no significant differences in values among the control and study groups for HGFs (P > .05). All tested BFSs decreased cell survival rates of M3T3C1 cells for 48 hours (P < .01), 72 hours (P < .001), and 96 hours (P < .001). Conclusion: Differences in the sensitivities of the 2 tested cell lines to the different BFSs might be the result of the different materials used to manufacture the screws. These results provide fundamental knowledge and new insights for the future design and development of new biocompatible BFSs for oral and maxillofacial surgery. (C) 2015 American Association of Oral and Maxillofacial SurgeonsÖğe DENTAL STEM CELL SOURCES AND THEIR POTENTIALS FOR BONE TISSUE ENGINEERING(Istanbul Univ Press, Istanbul Univ Rectorate, 2015) Asutay, Fatih; Acar, Ahmet H.; Yolcu, Umit; Kirtay, Mustafa; Alan, HilalTissue engineering arouses excitement in all medical fields that deal with bone healing. The ultimate aim of these approaches are to shorten the healing process, obtain highly differentiated functional tissues and eliminate the need for a second surgical site required for autogenous bone grafts. Mesenchymal stem cells have been increasingly used in the experiments which were conducted in these fields and the results are promising. Dental stem cells have come to the forefront both because of their relative ease of access and also their superior characteristics. This article investigates the importance of dental stem cells for bone tissue engineering and their regeneration potentials.Öğe DENTAL STEM CELL SOURCES AND THEIR POTENTIALS FORBONE TISSUE ENGINEERING(Journal of Istanbul University Faculty of Dentistry, 2015) Asutay, Fatih; Acar, Ahmet H.; Yolcu, Ümit; Kırtay, Mustafa; Alan, HilalÖz: Doku mühendisliği yaklaşımları, kemik iyileşmesi ile ilgilenen her tıp alanında heyecan uyandırmaktadır. İyileşme sürelerinin kısalması, daha kaliteli ve fonksiyonel doku elde edilmesi ve ikincil bir cerrahi sahaya gereksinimi ortadan kaldırması bu yaklaşımların nihai hedeflerindendir. Mezenşimal kök hücreler bu alandaki çalışmalarda giderek artarak kullanılmakta ve sonuçlar umut vermektedir. Dental kök hücreler de hem kolay temin edilebilmesi hem de üstün özellikleri sebebiyle ön plana çıkmaktadırlar. Bu çalışmada dental kök hücrelerin kemik doku mühendisliği yaklaşımları için önemi ve rejenerasyon potansiyelleri incelenmiştirÖğe Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS(Taylor & Francis Ltd, 2017) Simsek, Neslihan; Akinci, Levent; Alan, Hilal; Gecor, Orhan; Ozan, UlkuFollowing root canal treatment, sealers may contact periapical tissue. The purpose of this study was to evaluate the systemic toxic effects of epoxy resin-based sealers (AH Plus and Obtuseal). Inductively coupled plasma-mass spectrometry (ICP-MS) was used to measure levels of trace elements (beryllium, magnesium, aluminium, calcium, chromium, arsenic and lead) in the brain, kidney and liver of rats. Twenty sterilized polyethylene tubes were then filled with AH Plus and Obtuseal and implanted into the dorsal subcutaneous tissue of 10 rats; three unoperated animals were used as a control group. After 45 days, the rats were sacrificed by cervical dislocation following anaesthesia, and brains, kidneys and livers were removed from all experimental animals. ICP-MS analysis was used to determine levels of trace elements. Data were analysed using Kruskal-Wallis and Connover post hoc tests. No significant differences were found in aluminium and calcium levels, but brains, kidneys and livers showed significantly higher amounts of magnesium and chromium than the corresponding controls. In the kidney and liver samples, arsenic levels were found to be higher than in the control group. Lead was detected at higher levels only in liver samples from the AH Plus group. Beryllium was not detected in any organ. It was concluded that AH Plus and Obtuseal release minimal quantities of trace elements when in contact with subcutaneous tissue, and further studies are needed to understand the systemic effects of these materials.Öğe Determination of trace elements in rat organs implanted with endodontic repair materials by ICP-MS(Springer, 2016) Simsek, Neslihan; Bulut, Eclin Tekin; Ahmetoglu, Fuat; Alan, HilalTo investigate the levels of seven elements using an inductively coupled plasma-mass spectrometry (ICP-MS) method in rat organs after the implantation of Micro Mega Mineral Trioxide Aggregate (MM-MTA), Bioaggregate (BA) and Biodentine (BD) materials. MM-MTA, BA and BD were implanted into the subcutaneous tissue of 15 Wistar albino rats; three control animals had no operation. After 45 days, the rats were sacrificed and their brains, kidneys and livers were removed. The ICP-MS analysis was used to determine trace elements. Data were analysed using the Kruskal-Wallis and Connover post hoc tests. There was no significant difference between the control groups and the MM-MTA, BA and BD groups according to the concentration of aluminum, calcium, arsenic and lead in the rats' organs. Beryllium was not detected in all tissue samples. Chromium levels of these materials were higher than the control group in brain and kidney samples (P = 0.038 and P = 0.037); magnesium levels were higher than the control group in kidney and liver samples (P = 0.030 and P = 0.008). MM-MTA, BA and BD were nontoxic according to trace element levels in brain, kidney and liver samples of rats. Further investigation is required to understand the systemic effects of these materials.Öğe Effects of low-level laser therapy following surgical extraction of the lower third molar with objective measurement of swelling using a three-dimensional system(Spandidos Publ Ltd, 2018) Koparal, Mahmut; Kucuk, Ayse Ozcan; Alan, Hilal; Asutay, Fatih; Avci, MehmetThe aim of the present study was to evaluate and compare the effects of single- and two-dose low-level laser therapy (LLLT) on the postoperative swelling, trismus and pain of patients undergoing extraction of impacted mandibular third molars. In addition, edema was volumetrically measured with a 3dMD face system. A total of 45 patients were randomly divided into three treatment groups (15 patients in each group) as follows: Group 1, receiving routine management with ice application and serving as the control group; Group 2, receiving a single dose of LLLT immediately following surgery; and Group 3, receiving two doses of LLLT, immediately following surgery and on day 2 after surgery. In the present study, a gallium-aluminum-arsenide (0.3 W, 40 sec, 4 J/cm(2)) diode laser device was applied extraorally at the insertion point of the masseter muscle. The trismus, pain level and facial swelling of the patients were evaluated. The visual analog scale (VAS) was used to examine the pain degree, while a 3dMD face photogrammetric system was used to evaluate the volumetric alterations of the swelling. The results indicated no statistically significant differences in the mean swelling or trismus among the three groups. The mean VAS measurements did not differ significantly among the groups at postoperative day 2; however, significantly reduced VAS values were observed in Group 2 compared with Group 1 at postoperative day 7 (P<0.05). The present study demonstrated that, although single-dose or two-dose LLLT had beneficial effects on the swelling, trismus and pain level, a significant reduction was only observed in the pain level at postoperative day 7.Öğe Effects of Potentilla fulgens as a Prophylactic Agent in Tibial Defects in Rats(Sci Printers & Publ Inc, 2016) Koparal, Mahmut; Irtegun, Sevgi; Alan, Hilal; Deveci, Engin; Gulsun, Belgin; Seker, UgurOBJECTIVE: To investigate the effects of Potentilla fulgens as a prophylactic agent on tibial defects in the rat. STUDY DESIGN: Twenty-eight male Wistar albino rats weighing 200-215 g each were divided into 3 experimental groups. The tibial bone defect group served as the control group. The experimental groups were Potentilla fulgens with tibial defect (14 days) and Potentilla fulgens with tibial defect (28 days). Extract of Potentilla fulgens was mixed with water (400 mg/kg/day) and given to groups 14 and 28 as drinking water. The histopathological and immunohistochemical characteristics of each tibial bone cavity within each group were observed. The trabecular new bone formation was evaluated by expression rate of osteonectin and osteopontin. RESULTS: In the Potentilla fulgens + tibial defect group (14 days), trabecular bone had started combining extensive new bone formation, osteocyte cells were evident, and lamellar bone was formed. Osteoblasts showed a positive reaction with osteonectin. Osteopontin expression was positively observed between fibrous structures and in the osteoblast and osteocyte cells. This can be considered indicative of new bone formation. In the Potentilla fulgens + tibial defect group (28 days), an increase in expansion in trabecular bone and myeloid tissue was observed. Osteoblastic activity and osteocyte cells began to be observed in new bone fragments. CONCLUSION: In our study we show that Potentilla fulgens extract provided a protective effect on new bone formation and aided in the development of osteocytes and secretion of matrix in osteoblasts. Additionally, we show the inductive effect of the extract on new bone formation. In particular, the expression of osteopontin and osteonectin was also supported with the Western blot technique on the development of osteoblasts and osteocytes, showing a similar trend with our results.Öğe The effects of recombinant human bone morphogenic protein-2 and low-level laser irradiation on synthetic graft healing in a rat bony defect model(E-Century Publishing Corp, 2016) Koparal, Mahmut; Kose, Ibrahim; Atalay, Yusuf; Cakmak, Omer; Alan, Hilal; Agacayak, Kamil Serkan; Erdogdu, Ibrahim HalilWe assessed the benefit of bone morphogenic protein-2 (BMP-2) and low-level laser irradiation (LLLI) on the osseointegration of synthetic bone grafts. Synthetic bone grafts are safe alternatives to autografts. BMP-2 and LLLI have been shown separately to improve bone healing. However, their effects have not been evaluated together with synthetic materials. Here, we used a rat model to compare the efficacy of BMP-2 and LLLI on synthetic graft healing. Male Sprague-Dawley rats (n = 35) were divided equally into groups: control (defect only), graft only, graft and BMP-2, graft and LLLI, and a combination of graft, LLLI, and BMP-2. A 3 x 3 mm monocortical tibial defect was filled using a synthetic graft with or without 2 mg/mL BMP-2 pre-treatment. LLLI was performed using an optical fibre and an indium-gallium-arsenide-phosphate laser for 60 s daily for 7 days, delivering 6.0 J/day. Histopathology was performed 4 weeks post-graft using a standardised scoring system (1-5, based on the degree of healing observed). Groups were compared using the MannWhitney U test, with p values < 0.05 considered significant. Osteogenesis was poor in both the control and graft-only groups (1.4 +/- 0.5 and 2.3 +/- 0.5, respectively). BMP-2-(4.3 +/- 0.5), LLLI-(3.7 +/- 0.5), and BMP-2/LLLI-treated (4.7 +/- 0.5) grafts all displayed significantly more healing than the control or graft alone groups (P < 0.001). Both BMP-2 and LLLI significantly improved the osseointegration of synthetic bone grafts. However, no synergy was noted between the therapies.Öğe Evaluating the Effect of Resveratrol on the Healing of Extraction Sockets in Cyclosporine A-Treated Rats(W B Saunders Co-Elsevier Inc, 2018) Ozcan-Kucuk, Ayse; Alan, Hilal; Gul, Mehmet; Yolcu, UmitPurpose: The objective of this study was to investigate the effects of resveratrol on alveolar socket healing after tooth extraction in normal and cyclosporin A (CsA)-treated rats. Materials and Methods: Seventy-two female Sprague-Dawley rats were separated into 4 groups of 18. Group 1 was injected with a placebo solution intraperitoneally. Group 2 was injected with resveratrol (10 mu mol/kg) intraperitoneally. Groups 3 and 4 were injected with CsA (10 mg/kg) subcutaneously for 8 days once daily before tooth extraction. Next, the teeth were extracted and CsA injection continued until the animals were sacrificed. Eight days after commencing the CsA injections, group 4 was injected with resveratrol while continuing with CsA injections. Nine rats from each group were sacrificed on days 14 and 28, and sections were examined to assess the degree of inflammation, formation of connective tissue, and new bone formation Immunohistochemical analysis was used to evaluate the alveolar socket healing process using osteocalcin and osteopontin markers. A P value less than .05 was considered significant. Results: There was more new bone formation in group 2 than in the other 3 groups on day 14 after tooth extraction (P < .05), and there was more new bone formation in group 2 than in groups 3 and 4 on day 28 after extraction (P < .05). Based on the immunohistochemical assessment, the amount of osteocalcin and osteopontin labeling was greater in group 2 compared with the other 3 groups on day 14 (P < .05); however, on day 28 after extraction, it was greater in group 4 compared with group 3 (P < .05). Conclusions: Resveratrol improves alveolar socket healing in normal and CsA-treated rats. Resveratrol also increases levels of osteocalcin and osteopontin in normal and CsA-treated rats. These results suggest that this natural compound is useful for alveolar socket healing after tooth extraction. (C) 2018 American Association of Oral and Maxillofacial SurgeonsÖğe Evaluation of the effects of the low-level laser therapy on swelling, pain, and trismus after removal of impacted lower third molar(Biomed Central Ltd, 2016) Alan, Hilal; Yolcu, Umit; Koparal, Mahmut; Ozgur, Cem; Ozturk, Seyit Ahmet; Malkoc, SiddikBackground: In current study we aimed to examine the effect of a low-level laser therapy on the pain, mouth opening and swelling of patients whose impacted 3rd molar tooth was extracted in addition measurement volumetrically to the edema with 3dMD face system. Methods: It was surveyed 15 patients who had bilateral symmetric lower 3rd molars. Surgical sides of patients were randomly separated into two groups: the study group and the control group. It was applied extra oral low-level laser therapy (LLLT, 0.3 W, 40 s, 4 J/cm(2)) to the study group (n = 15) after the surgical operation and on the 2nd day. Only routine postoperative recommendation (ice application) was made in the control (n = 15) group. The maximum mouth opening, pain level and facial swelling evaluated. 3dMD Face r (3dMD, Atlanta, GA) Photogrammetric System was used to evaluate volumetric changes of the swelling. Results: There was no statistically significant difference in the edema and interincisal opening between the groups and the pain level in the laser group was significantly lower than in the control group on the 7th postoperative day. Conclusions: Although there were decreasing trismus, swelling, and pain level, with this LLLT, there was significant difference only in the 7th day pain level in the laser group compared with the control group.Öğe Farklı Kaynaklardan Elde Edilen Mezenşimal Kök Hücrelerin ve Konsantre Büyüme Faktörünün Tavşan Temporomandibular Ekleminde Oluşturulan Osteoartrit Modellerindeki Etkinliklerinin Araştırılması(2017) Yolcu, Ümit; Polat, Serkan; Alan, Hilal; Gül, MehmetOsteoartrit (OA), artiküler kartilajda fibrilasyon ve erozyona, alttaki subkondral kemikte dejenerasyona sebep olan inflamatuar bir durumu tanımlar. Patogenezinde inflamasyon, biyomekanik ve immunolojik reaksiyonlar da rol oynarlar. TME OA?ları, şiddetli ağrı ve fonksiyon bozukluklarıyla karakterize olup TME bozukluklarının bir alt grubudur. OA etkisiyle kartilajın dejenere olması sonucu artiküler yüzeyler arasında sürtünme artar, bunun sonucunda eklem hareketleri bozulabilmekte ve kartilaj, kapsül, ligamentler, sinoviyal membran, subkondral kemik ve kaslar gibi ekleme komşu dokularda patolojik cevaplar oluşabilmektedir. TME OA?sının tipik bulguları arasında eklemden gelen klik sesi ve krepitus, eklem ve kas hassasiyeti, ağız açılması esnasında çenenin deviasyonu ve ağız açılmasındaki ciddi kısıtlılık gibi bulgular sayılabilir. Son yıllarda özellikle ortopedi alanında, OA?nın en sık etkilediği eklem olan diz eklemi içine uygulanan Trombositten Zengin Plazma (TZP) OA tedavisinde popüler hale gelmiştir. TZP ve Trombositten Zengin Fibrin (TZF) normal kandan 8-10 kat daha fazla trombosit ve bununla birlikte birçok büyüme faktörü içerir. Analjezik, inflamasyon baskılama ve anjiogenezisi stimule edici özellikleri vardır. Son yıllarda TZP ve TZF?den daha yoğun büyüme faktörü ve trombosit içeren konsantre büyüme faktörü (KBF) üzerinde çalışmalar yapılmıştır. TZP ve TZF?ten farklı olarak KBF, venöz kanın sabit bir sıcaklıkta değişik hızlarda özel bir cihaz tarafından santrifüj edilmesiyle elde edilir. OA'da destrükte olan kıkırdak ve kemik dokuların rejenerasyonu için eklem içine kök hücre uygulamaları oldukça popüler hale gelmiştir. Mezenşimal kök hücreleri yetişkin kemik iliğinde yer alan multipotent hücrelerdir. Bu hücreler undiferansiye hücrelere replike olabilirler ve kıkırdak, kemik ve yağ gibi birçok mezenşimal hücreye diferansiye olabilme potansiyelleri vardır. Bu projenitör hücrelerin elde edilebileceği kemik iliği, dental pulpa, yağ dokusu, sinoviyum, periosteum, umbilikal kord, kas doku ve periferal kan gibi birçok kaynak vardır. TME'yi etkileyen OA ve diğer dejeneratif hastalıklarda kök hücre uygulamalarının etkinliğini inceleyen çalışmalar henüz yerlerini almaktadırlar. Bu çalışmada TME?de kemik ve kıkırdak harabiyeti ile sonuçlanan OA'larda multipotent olan ve diferansiasyon özelliği yüksek olan mezenşimal kök hücrelerin kıkırdak ve kemik hücre prekürsörlerine diferansiye olabilme ve KBF'nin içerdiği yoğun büyüme faktörleri ve doku iyileşmesini stimule edici özelliklerinden yararlanmak istedik. Çalışmamızda monoiodoasetat (MIA) ile artiküler kartilaj, osteokondral birleşim ve subkondral kemikte destrüksiyon elde edildi. MIA enjeksiyonundan 4 hafta sonra eklem içine artrosentez yöntemi ile medium içerisinde mezenşimal kaynaklı kök hücrelerin (dental pulpa ve yağ doku kaynaklı) ve likit KBF enjekte edildi. Sonuçlar histomorfometrik ve immünohistokimyasal olarak incelenip kıkırdak ve kemik dokudaki değişimler değerlendirildi. OA grubunun toplam hasar skoru, KBF, adipoz, dental pulpa ve medium gruplarından anlamlı düzeyde yüksek bulundu. Adipoz grubunun toplam skoru, dental pulpa ve medium gruplarından anlamlı düzeyde düşük bulundu. KBF ve adipoz grupları arasında toplam skor açısından anlamlı bir farklılık bulunmadı. Bu çalışma KBF, adipoz doku ve dental pulpa kaynaklı kök hücrelerin TME OA tedavisi üzerine olumlu etkilere sahip olduğunu göstermiştir.Öğe Human papilloma virus(Turgut Özal Tıp Merkezi Dergisi, 2016) Koparal, Mahmut; Alan, Hilal; Gündüz, Derya Toprak; Gülsün, Belgin; Ünsal, Haluk Yener; Erdoğdu, Halil İbrahimAbstract Human papillomavirus (HPV) is a double-helix DNA virus in the papillomaviridae family. HPV has an affinity for epithelial tissue and causes benign and malignant changes to the stratified epithelium of the epidermis and mucous membranes. HPV is an etiological factor in many benign and malignant lesions of the head, neck, urogenital organs, skin, and mucous membranes. The role of HPV in the pathogenicity of squamous cell carcinoma in the head and neck area was first recognised in 1983 after findings showed histopathologic similarities between oral cancer and HPV infection. Because HPV has been implicated as the etiological factor for oral squamous cell carcinoma, HPV infection can be regarded as a subgroup of cancers affecting the oropharyngeal region. Due to these characteristics, HPV infection has been of particular interest in the field of maxillofacial surgery and dentistry. Screening, oral findings, early diagnosis, and proper treatment are of paramount importance in many HPVrelated infections. Keywords: Human Papillomavirus; HPV; Oral Cancer.
