Yazar "Atalan E." seçeneğine göre listele

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    Identification of biodiversity of some Streptomyces species and determination of a restriction fragment length polymorphism (RFLP) profile of 16S rDNA gene region
    (Medwell Journals, 2014) Ozdemir K.; Ogun E.; Ertas M.; Acar S.; Atalan E.
    In this study, soil samples from the roots and peripheral regions of some agricultural plant species growing in the Van region (such as sainfoin, bean, malus, wheat and sugar beet) were collected between May and June 2004 and 139 strains of Streptomyces bacteria were isolated from these samples. Moreover, researchers determined the physicochemical characteristics of these soil samples. Streptomyces bacteria were color-grouped and the Diaminopimelic Acid (DAP) forms were detected followed by the determination of then phenotypic characteristics. When these isolates were divided into 39 color groups, 20 strains were found to contain L-DAP and therefore were confirmed as members of the genus Streptomyces. The species were identified using the TAXON Software and similarity dendrograms were constructed by the Unweighted Pair-Group Method with an Arithmetic average (UPGMA) cluster analysis using the Multi-Variate Statistical Package (MVSP 3.1). Genomic DNA was isolated from these bacteria and 16S rDNA gene was amplified by PCPv followed by restriction digestion with Bspl43I, Haelll, Mnll and SphI for Restriction Fragment Length Polymorphism (RFLP) analysis. In the dendrogram obtained at the end of the RFLP analysis, four groups of two or more members and six 1 -member groups were established according to the similarity ratio criterion. © Medwell Journals, 2014.
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    In vitro callus propagation and antibacterial activities of callus an edible endemic and medicinal plant Scutellaria orientalis L. Subsp. bicolor
    (Mattioli 1885 S.p.A., 2016) Ozdemir F.A.; Kilic O.; Atalan E.
    Plants are a stupendous source for exploration of new medicinal products for drug development. Today several drugs used to treat many diseases are simply synthetic modifications or copies of naturally obtained plant substances or extracts. Medicinal plants offer best source to obtain a variety of new drugs; and new plants need to be investigated for their potential use against various microorganisms including bacteria. Scutellaria orientalis subsp. bicolor in the Lamiaceae (mint) family, is an endemic plant species that grows widely on abondoned lands at altitude of 1000-2800 meters in Eastern Anatolia. The plant or extracts are popularly used in preparation of various traditional medicines to treat many diseases including stress, breast and ovarian cancers; however there is need to establish validity of these methodologies scientifically as plant tissue culture techniques could serve as an alternative way to produce them. This study aimed to documents crude callus extracts from hypocotyl explants obtained from 17 days old plantlets of Scutellaria orientalis subsp. bicolor for antibacterial activities. Research findings clearly showed inhibition of growth of Staphylococcus aureus COWAN 1 and Bacillus subtilis var. Niger ATCC 10 in the extracts determined by disc diffusion methodology. Antibacterial activity was evaluated by measuring zone diameters in mm. No antibacterial activities were detected in control group. The hexane, acetone and methanol extracts of S. orientalis subsp. bicolor calli exhibited inhibitory action against both bacterial strains. Moreover, callus extracts showed less anti bacterial activities on calli developed on MS medium containing NAA compared to calli obtained on MS medium containing BAP. Furthermore, the results confirmed inhibitory effects of increased BAP concentrations on callus weight, size and quantity of extracts. Moreover, methanol extracts showed more antibacterial activity compared to acetone and hexane extracts. Better knowledge about antibacterial activities of this endemic plant sub species could be highly useful for understanding ways for cheap, commercial production of these extracts at extensive level under controlled conditions. © Mattioli 1885.
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    Use of random amplified polymorphic DNA PCR to distinguish Micromonospora species isolated from soil
    (Medwell Journals, 2014) Ertas M.; Ozdemir K.; Atalan E.
    Recently with the advancement of molecular methods, studies that determine intraspecific genetic polymorphisms have yielded substantial results. The aim of this study was to use Random Amplified Polymorphic DNA PCR (RAPD-PCR) analysis to determine the differences among Micromonospora species isolated from the soil. M3 medium with antibiotics was used to isolate the species from the soil and 65 Micromonospora species were identified. Total DNA analysis was performed for the different color groups which were obtained as a result analysis of the species. Sm5R, Sm6F, DAF4, Ml 3, 27F and 1492R primers were used for RAPD-PCR analysis and the band profiles of the species were obtained. The dendrogram generated according to the simple matching coefficient method clearly showed the differences detected among the species. A total of seven groups were identified along with two main groups. Five of these groups had a single member, one had 3 and one had 2 members. © Medwell Journals, 2014.