Use of random amplified polymorphic DNA PCR to distinguish Micromonospora species isolated from soil
Küçük Resim Yok
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Medwell Journals
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Recently with the advancement of molecular methods, studies that determine intraspecific genetic polymorphisms have yielded substantial results. The aim of this study was to use Random Amplified Polymorphic DNA PCR (RAPD-PCR) analysis to determine the differences among Micromonospora species isolated from the soil. M3 medium with antibiotics was used to isolate the species from the soil and 65 Micromonospora species were identified. Total DNA analysis was performed for the different color groups which were obtained as a result analysis of the species. Sm5R, Sm6F, DAF4, Ml 3, 27F and 1492R primers were used for RAPD-PCR analysis and the band profiles of the species were obtained. The dendrogram generated according to the simple matching coefficient method clearly showed the differences detected among the species. A total of seven groups were identified along with two main groups. Five of these groups had a single member, one had 3 and one had 2 members. © Medwell Journals, 2014.
Açıklama
Anahtar Kelimeler
Genetic polymorphism, Micromonospora, RAPD-PCR, Simple matching coefficient, Soil
Kaynak
Journal of Animal and Veterinary Advances
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
13
Sayı
16
