Use of random amplified polymorphic DNA PCR to distinguish Micromonospora species isolated from soil

dc.authorscopusid56385361600
dc.authorscopusid25230283000
dc.authorscopusid6602206186
dc.contributor.authorErtas M.
dc.contributor.authorOzdemir K.
dc.contributor.authorAtalan E.
dc.date.accessioned2024-08-04T20:02:33Z
dc.date.available2024-08-04T20:02:33Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractRecently with the advancement of molecular methods, studies that determine intraspecific genetic polymorphisms have yielded substantial results. The aim of this study was to use Random Amplified Polymorphic DNA PCR (RAPD-PCR) analysis to determine the differences among Micromonospora species isolated from the soil. M3 medium with antibiotics was used to isolate the species from the soil and 65 Micromonospora species were identified. Total DNA analysis was performed for the different color groups which were obtained as a result analysis of the species. Sm5R, Sm6F, DAF4, Ml 3, 27F and 1492R primers were used for RAPD-PCR analysis and the band profiles of the species were obtained. The dendrogram generated according to the simple matching coefficient method clearly showed the differences detected among the species. A total of seven groups were identified along with two main groups. Five of these groups had a single member, one had 3 and one had 2 members. © Medwell Journals, 2014.en_US
dc.identifier.doi10.3923/javaa.2014.974.977
dc.identifier.endpage977en_US
dc.identifier.issn1680-5593
dc.identifier.issue16en_US
dc.identifier.scopus2-s2.0-84907979836en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage974en_US
dc.identifier.urihttps://doi.org/10.3923/javaa.2014.974.977
dc.identifier.urihttps://hdl.handle.net/11616/91767
dc.identifier.volume13en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMedwell Journalsen_US
dc.relation.ispartofJournal of Animal and Veterinary Advancesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGenetic polymorphismen_US
dc.subjectMicromonosporaen_US
dc.subjectRAPD-PCRen_US
dc.subjectSimple matching coefficienten_US
dc.subjectSoilen_US
dc.titleUse of random amplified polymorphic DNA PCR to distinguish Micromonospora species isolated from soilen_US
dc.typeArticleen_US

Dosyalar