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    Detection of extended spectrum beta-lactamases and antibiotic susceptibilities of Klebsiella pneumoniae strains
    (2001) Tekereko?lu M.S.; Ayan M.; Otlu B.; Taştekin N.; A?el H.E.; Durmaz B.; Özerol I.H.
    One hundred Klebsiella pneumoniae strains isolated from various clinical specimens were investigated for the presence of extended spectrum beta-lactamases (ESBL) and the antimicrobial susceptibility patterns. The ESBL production was detected in 44% of strains isolated from inpatients and 14% of strains isolated from outpatients. The antimicrobial susceptibility rates of the total 100 strains and ESBL positive 58 strains were found to be as follows respectively: Cephalotin and cefuroxime 40% and 0%, cefoxitin 78% and 100%, ceftazidime 48% and 20%, cefotaxime 56% and 20%, ceftriaksone 52% and 18%, gentamicin 66% and 40%, amikacin 70% and 65%, ciprofloxacin 88% and 90%, imipenem 80% and 90%, meropenem 100% and 100%, aztreonam 50% and 30%, amoxicillin-clavulonic acid 28% and 10%, and trimethoprimsulphametoxazole 72% and 40 percent. The results indicated that ciprofloxacin, imipenem and meropenem might be used in the therapy of infections due to K. pneumoniae.
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    Epidemiological analysis of Staphylococcus aureus strains from nasal carriers in a teaching hospital
    (2002) Guducuoglu H.; Ayan M.; Durmaz R.; Berktas M.; Bozkurt H.; Bayram Y.
    The present study was conducted to assess the epidemiological relation of Staphylococcus aureus isolates from nasal carriers of hospital staff. Nasal swabs were taken from each of 327 personnel. After culturing on blood agar for overnight, probable staphylococcal isolates were identified and subjected to tube coagulase test. After a two-week interval, second nasal swabs were taken from the subjects whose first cultures were positive for S. aureus. Nasal carriage was defined in 58 (17.7%) personnel with positive culture for both sampling time. Antibiogram typing and arbitrarily-primed polymerase chain reaction (AP-PCR) with M13 primer were used for typing of the strains. Antibiotyping distinguished seven types and three subtypes, and 85% of the isolates were clustered in one group. AP-PCR, in contrast, identified 12 distinct patterns with 13 variants. A specific profile was not found among the isolates obtained from the personnel in a particular clinic. These results indicate that antibiotyping has poor discrimination power and heterogeneity among the nasal S. aureus strains in the hospital personnel screened is high.