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The beneficial effects of 18?-glycyrrhetinic acid following oxidative and neuronal damage in brain tissue caused by global cerebral ischemia/reperfusion in a C57BL/J6 mouse model
(Springer-Verlag Italia Srl, 2014) Oztanir, M. Namik; Ciftci, Osman; Cetin, Asli; Durak, M. Akif; Basak, Nese; Akyuva, Yener
This study investigated the effects of 18 beta-glycyrrhetinic acid (GA) on neuronal damage in brain tissue caused by global cerebral ischemia/reperfusion (I/R) in C57BL/J6 mice. All subjects (n = 40) were equally divided into four groups: (1) sham-operated (SH), (2) I/R, (3) GA, and (4) GA+I/R. The SH group was used as a control. In the I/R group, the bilateral carotid arteries were clipped for 15 min, and the mice were treated with the vehicle for 10 days. In the GA group, mice were given GA (100 mg/kg) for 10 days following a median incision without carotid occlusion. In the GA+I/R group, the I/R model was applied to the mice exactly as in the I/R group, and they were then treated with the same dose of GA for 10 days. Cerebral I/R significantly induced oxidative stress via an increase in lipid peroxidaitons and a decrease in elements of the antioxidant defense systems. However, GA treatment was protective against the oxidative effects of I/R by inducing significant increases in antioxidant defense systems and a significant decrease of lipid peroxidations. Additionally, cerebral I/R increased the incidence of histopathological damage and apoptosis in brain tissue, but these neurodegenerative effects were eliminated by GA treatment. Therefore, the current study demonstrated that GA treatment effectively prevents oxidative and histological damage in the brain caused by global I/R. In this context, GA may be useful for the attenuation of the negative effects of global cerebral I/R and, in the future, it may be a viable and safe alternative treatment for ischemic stroke in humans.
Beneficial effects of ?-glucan against cisplatin side effects on the nervous system in rats
(Acta Cirurgica Brasileira, 2016) Kaya, Kursat; Ciftci, Osman; Cetin, Asli; Tecellioglu, Mehmet; Basak, Nese
PURPOSE: To investigate the protective effect of Bg on cisplatin (CP)-induced neurotoxicity in rats. METHODS: Twenty eight rats were randomly distributed into four groups. The first group was kept as a control. In the second group, CP was given at the single dose of 7 mg/kg intraperitoneally. In the third group, beta g was orally administered at the dose of 50 mg/kg/day for 14 days. In the fourth group, CP and beta g were given together at the same doses. RESULTS: CP treatment caused significant oxidative damage via induction of lipid peroxidation and reductions antioxidant defense system potency in the brain tissue. In addition, histopathological damage increased with CP treatment. On the other hand, beta g treatment largely prevented oxidative and histopathological negative effects of CP. CONCLUSIONS: Cisplatin has severe neurotoxic effects in rats and ag supplementation has significant beneficial effects against CP toxicity depending on its antioxidant properties. Thus, it appears that Ag might be useful against CP toxicity in patients with cancer in terms of nervous system.
Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model
(Bmc, 2018) Melekoglu, Rauf; Ciftci, Osman; Eraslan, Sevil; Cetin, Asli; Basak, Nese
Background: In recent years, cancer rates have been rising among reproductive-age women. Thus, chemotherapy exposure has become an important cause of premature ovarian failure (POF). There has been growing interest regarding the preservation and restoration of ovarian function before and after oncological treatment because of the reproductive risk of chemotherapeutics and improved long-term survival of cancer patients. In this study, we sought to analyze the effects of curcumin (CRC) and capsaicin (CPS) on cyclophosphamide-induced POF in a rat model. Methods: POF in rats was induced by intraperitoneal injection of 200 mg/kg cyclophosphamide on day 1 and then 8 mg/kg/day for the following 14 days. After 14 days of cyclophosphamide administration, rats were randomly divided into three groups as follows (n = 10/group): POF, POF + CRC (100 mg/kg/day), and POF + CPS (0.5 mg/kg/day) to determine the effects of CRC and CPS on the cyclophosphamide-induced POF rat model. Biochemical, hormonal, and histopathological evaluations were performed on blood and tissue samples 14 days after the CRC and CPS treatments. Results: Malonaldehyde levels were significantly reduced, and glutathione levels and superoxide dismutase activity were significantly increased, in ovarian tissues in the POF + CRC and POF + CPS groups compared with the POF group. In the POF group, we observed hemorrhage and prominent mononuclear cell infiltration beneath the germinative epithelium, vascular congestion in ovarian stroma, hemorrhage around the corpus luteum, and atresia in ovarian follicles. This histopathological damage was significantly improved by treatment with CRC and CPS. There was a significant reduction in serum follicle-stimulating hormone and luteinizing hormone levels in rats treated with CRC and CPS compared with the POF group. Moreover, the levels of estradiol and anti-mullerian hormone in rats treated with CRC and CPS were significantly increased compared with the control group. Conclusions: In conclusion, CRC and CPS treatment of rats with cyclophosphamide-induced POF had a beneficial effect on reducing ovarian damage by improving tissue oxidative stress marker levels, ovarian reserve marker levels, and histopathological parameters. The significant improvements in ovarian tissue histopathological damage and hormonal levels detected in this study indicate that treatment with CRC or CPS might be a conservative treatment approach for cyclophosphamide-induced POF.
The beneficial effects of Montelukast against 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity in female reproductive system in rats
(Acta Cirurgica Brasileira, 2016) Melekoglu, Rauf; Ciftci, Osman; Cetin, Ash; Basak, Nese; Celik, Ebru
PURPOSE: To determine the toxic effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on reproductive system and the beneficial effects of Montelukast (ML) with histological and biochemical analysis. METHODS: Rats were randomly divided into four equal groups (control, TCDD, ML and TCDD+ML). Tissue samples were collected on day 60 and oxidative status and histological alterations were analyzed. RESULTS: The results showed a significant increase in oxidative and histological damage on uterine and ovarian tissues. Otherwise, the oxidative and histological damages caused by TCDD were prevented with ML treatment. CONCLUSION: The toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on female reproductive system were reversed with Montelukast treatment. Therefore, we claimed that ML treatment might be useful for TCDD toxicity.
The beneficial effects of nerolidol and hesperidin on surgically induced endometriosis in a rat model
(Taylor & Francis Ltd, 2018) Melekoglu, Rauf; Ciftci, Osman; Eraslan, Sevil; Cetin, Asli; Basak, Nese
The objective of this article is to analyze the effects of nerolidol and hesperidin treatment on surgically induced endometriosis in a rat model. Endometriosis was induced in 24 healthy adult female Wistar albino rats via homologous uterine horn transplantation. Three operations were performed on each rat. After the second operation, the rats were randomized into control, nerolidol, and hesperidin treatment groups, and medications were administered for 2 weeks. The effects of the drugs on the endometriotic foci were evaluated after the third operation. Compared with the endometriosis control group, the average volume of the lesions was significantly lower in rats treated with hesperidin and nerolidol. Malondialdehyde levels were significantly reduced in the nerolidol-treated group, and glutathione levels and superoxide dismutase activity were significantly elevated in the endometriotic foci of both the hesperidin- and nerolidol-treated groups compared with the endometriosis group. Hesperidin and nerolidol treatment also improved histological parameters, such as hemorrhage, vascular congestion, necrosis, and inflammatory cell infiltration in the endometriotic foci. The results of this study demonstrated that treatment with the potent antioxidants nerolidol and hesperidin caused a significant regression of surgically induced endometriotic foci in rats.
The beneficial effects of nerolidol and hesperidin on surgically induced endometriosis in a rat model
(Gynecologıcal endocrınology, 2018) Melekoğlu, Rauf; Çiftlikci, Osman; Eraslan, Sevil; Çetin, Aslı; Basak, Nese
The objective of this article is to analyze the effects of nerolidol and hesperidin treatment on surgically induced endometriosis in a rat model. Endometriosis was induced in 24 healthy adult female Wistar albino rats via homologous uterine horn transplantation. Three operations were performed on each rat. After the second operation, the rats were randomized into control, nerolidol, and hesperidin treatment groups, and medications were administered for 2 weeks. The effects of the drugs on the endometriotic foci were evaluated after the third operation. Compared with the endometriosis control group, the average volume of the lesions was significantly lower in rats treated with hesperidin and nerolidol. Malondialdehyde levels were significantly reduced in the nerolidol-treated group, and glutathione levels and superoxide dismutase activity were significantly elevated in the endometriotic foci of both the hesperidin- and nerolidol-treated groups compared with the endometriosis group. Hesperidin and nerolidol treatment also improved histological parameters, such as hemorrhage, vascular congestion, necrosis, and inflammatory cell infiltration in the endometriotic foci. The results of this study demonstrated that treatment with the potent antioxidants nerolidol and hesperidin caused a significant regression of surgically induced endometriotic foci in rats.
The Effects of Body Mass Index on Second-Trimester Amniotic Fluid Cytokine and Matrix Metalloproteinase Levels
(Karger, 2018) Melekoglu, Rauf; Ciftci, Osman; Eraslan, Sevil; Basak, Nese; Celik, Ebru
Aim: The aim of this study was to determine the effects of obesity on amniotic fluid (AF) inflammatory markers in second-trimester AF, testing the hypothesis that there is a relationship between maternal body mass index (BMI) and fetal inflammatory exposure. Methods: AF was obtained from 84 singleton pregnant women undergoing elective amniocentesis for karyotype analysis at 16-24 weeks of gestation between April 2014 and May 2016. The cell-free AF was used to analyze interleukin (IL)-1 beta and IL-6, and matrix metalloproteinase (MMP)-1, MMP-6, and MMP-13. Results: IL-1 beta levels were significantly higher in class II-III obese patients than in class I obese, overweight, and normal weight patients (14.68 +/- 1.37 vs. 13.34 +/- 1.86 vs. 13.00 +/- 2.22 vs. 10.78 +/- 1.92, respectively; p < 0.05). IL-6 levels were lowest in the normal weight group and highest in class II-III obese patients. MMP-1, MMP-6, and MMP-13 levels were also significantly higher in class II-III obese patients than in the other groups. Conclusion: This study demonstrated that the fetuses of class II-III obese women are exposed in utero to higher cytokine and MMP levels than fetuses of lean women. Modification of current cutoff levels of intra-amniotic cytokines and MMPs according to the BMI could improve the accuracy of the prenatal diagnosis of intra-amniotic infection and inflammation. (C) 2017 S. Karger AG, Basel
THE EFFECTS OF CHRYSIN ON BURN HEALING
(Carbone Editore, 2021) Turtay, Muhammet Gokhan; Ciftci, Osman; Cetin, Asli; Gurbuz, Sukru; Oguzturk, Hakan; Basak, Nese; Firat, Cemal
Introduction: This study aims to investigate the effects of chrysin on burn healing in burned model rats. Materials and methods: Rats were burned and randomly divided into four groups. Group 1; the group whose burn wound was left to secondary healing. Group 2; the group to which bacitracin neomycin sulfate pomade was topically administered. Group 3; the group to which chrysin was administered topically and with gastric gavage. Group 4; the group was assigned as the group to which chrysin and bacitracin neomycin sulfate was administered. Results: On days 3, 7 and 14, blood samples and skin biopsies were taken. Necrosis, congestion, hemorrhage and IL-1 beta were found to have the lowest levels on all days when bacitracin and chrysin were administered together. This group was also found to have the lowest level of TNF-alpha serum levels on days 7 and 14. Conclusion: We conclude that chrysin is effective in the treatment of burn wounds when used separately, but when combined with topical bacitracin pomade application, it is more effective for healing.
The efficacy of hesperidin for treatment of acute otitis media
(Elsevier Sci Ltd, 2019) Cetinkaya, Erdem Atalay; Ciftci, Osman; Alan, Saadet; Oztanir, M. Namik; Basak, Nese
Objectives: In this experimental study, the effect of hesperidin on the treatment of acute otitis media (AOM) was investigated in an AOM-induced rat model. Methods: In total, 35 rats were randomly divided into the following five groups (n = 7): group 1 (control), group 2 (AOM with no treatment), group 3 (AOM + antibiotic), group 4 (AOM + hesperidin), and group 5 (AOM + hesperidin + antibiotic). On day 14, group 3,4 and 5 rats were given antibiotic and hesperidin via gavages, respectively. Histopathological and immunological analyses were performed and the results analyzed. Results: Serum levels of TNE-alpha, IL-4, IL-6 and IL-beta were significantly decreased in the hesperidin- and antibiotic-treated groups compared to the AOM group. The AOM + antibiotic and AOM + hesperidin groups demonstrated reduced histological damage compared to the AOM group. Between the AOM + antibiotic and AOM + hesperidin groups, significant differences in tympanic membrane thickness(ThicTM), inflammation(Inf), and sclerosis(Sc) values were observed. However, no difference in epithelial damage(DamEpith), was seen between the two groups. There was a significant difference in the AOM + antibiotic and AOM + antibiotic + hesperidin groups compared to AOM group (P < 0.001). Conclusions: In this study, we observed that both antibiotic and hesperidin treatment reduced AOM symptoms in an AOM-induced rat model. The values in AOM + antibiotic + hesperidin group were markedly lower than those of the other groups. From our results, we propose that hesperidin, in combination with antibiotics, may provide a successful alternative treatment for AOM compared with antibiotics used alone. (C) 2018 Elsevier B.V. All rights reserved.
Favourable effect of ?-glucan treatment against cisplatin-induced reproductive system damage in male rats
(Wiley, 2019) Kaya, Kursat; Ciftci, Osman; Aydin, Muhterem; Cetin, Asli; Basak, Nese
The aim of this study was to investigate the potential beneficial effects of beta-glucan treatment against oxidative, histological and spermatological damage caused by cisplatin on the male reproductive system. Twenty-eight Sprague Dawley male rats were used in the study. The rats were randomly divided into four equal-sized groups: a control group, cisplatin group (7 mg/kg in a single-dose cisplatin administered intraperitoneally), beta-glucan group (beta-glucan given at a dose of 50 mg kg(-1) d(-1) for 14 day) and a cisplatin plus beta-glucan group (cisplatin and beta-glucan administered together at the same dose). Cisplatin administration induced an increase in the level of thiobarbituric acid-reactive substances, a lipid peroxidation indicator. It induced a decrease in enzymatic (superoxide dismutase, catalase and glutathione peroxidase) activities and nonenzymatic (reduced glutathione) antioxidant levels. In addition, cisplatin caused both histological and spermatological damage, as shown by a decrease in sperm motility and epididymal sperm concentrations and an increase in abnormal sperm rates. The beta-glucan treatment improved cisplatin-induced oxidative, histological and spermatological damage. This study revealed that beta-glucan treatment provided prevention against male reproductive system damage caused by cisplatin. These preventative effects were likely due to its antioxidant properties.
Hesperidin, a Citrus Flavonoid, Has the Ameliorative Effects Against Experimental Autoimmune Encephalomyelitis (EAE) in a C57BL/J6 Mouse Model
(Springer/Plenum Publishers, 2015) Ciftci, Osman; Ozcan, Cemal; Kamisli, Ozden; Cetin, Asli; Basak, Nese; Aytac, Bilal
The aim of this study was determined the effects of Hesperidin (HP) on neuronal damage in brain tissue caused by Experimental allergic encephalomyelitis (EAE), an established model of multiple sclerosis in C57BL/J6 mice. To explore 40 mice were equally divided into four groups: (1) Control, (2) EAE, (3) HP, and (4) HP + EAE. 14 days after induction of EAE with MOG35-55 and pertussis toxin, the mice treated with HP at the doses of 50 mg/kg/day for 7 days subcutaneously. To our results HP treatment prevents the oxidative stress caused by EAE via a decrease in lipid peroxidations and increase in elements of the antioxidant defense systems in brain tissue. Also, EAE elevate the IL-17, express the pro-inflammatory cytokines, and caspase-3-like immunreactivity, show apoptosis, staining in EAE mice brain and increased the incidence of histopathological damage. However, immonohistochemical and histological changes were reversed with HP. Moreover, elevated TNF-alpha and IL-1 beta levels, a result of EAE, were decreased in serum and neurological deficits as clinical signs were reversed with HP treatment in EAE mice, given HP. In conclusion, HP treatment effectively prevents oxidative, immunological and histological damage in the brain caused by EAE. It was thought that the beneficial effects of HP are likely a result of its strong antioxidant and anti-inflammatory properties.
INVESTIGATING THE THERAPEUTIC EFFECTS OF FLAVONOID-STRUCTURED HESPERIDIN IN ACUTE BURN TRAUMAS
(Parlar Scientific Publications (P S P), 2018) Oguzturk, Hakan; Ciftci, Osman; Taslidere, Asli; Turtay, Muhammet Gokhan; Gurbuz, Sukru; Basak, Nese; Firat, Cemal
One of the flavonoids, Hesperidin might be beneficial in the treatment of burn injuries. In this study, it was aimed to investigate the effect of Hesperidin in the treatment of burn injuries. In this study, 32 male Sprague Dawley rats were randomized to four groups after local burn development: Group I (Control); rats were left to secondary healing without treatment, Group 2 (Bacitracin group); rats were treated with pomade locally (Bacitracin neomycin sulfate), Group 3(Hesperidin) were treated with Hesperidin for 14 days without Bacitracin, and Group 4(Bacitracin+Hesperidin); local pomade treatment and Hesperidin 50 mg/kg were administered by gastric gavage once a day for 14 days. Skin biopsies and blood samples were collected on days 3, 7,14 relative to burn induction. IL-1 beta and TNF-alpha studies from blood samples and histopathological inspections from tissue biopsies were performed. In Hesperidin + Bacitracin group, 3rd, 7th and 14th days significant reduction in necrosis in the epidermis and dermis, in the 3rd and 14th days little mononuclear cell infiltration that were detected. In this group, on day 14, mononuclear cell infiltration decreased significantly and fairly small amount of vascular congestion was observed on day 7. On day 14 of Hesperidin+Bacitracin group, no congestion or hemorrhage was observed. According to histopathological inspection, it was observed that Hesperidin is effective in burn injury treatment but the best results were observed in Hesperidin+Bacitracin group. It was detected that the most significant decrease in IL-1 beta and TNF-alpha levels in time was in Hesperidin+Bacitracin group. In conclusion, Hesperidin was effective on burn wound healing. Moreover, the effect could be amplified when combined with topical antibiotics in the early stage of burn wound healing.
A NOVEL SCHIFF BASE DERIVATIVE FOR EFFECTIVE TREATMENT OF AZOXYMETHANE INDUCED COLON CANCER
(Int Journal Pharmaceutical Sciences & Research, 2014) Dogan, Aysegul; Basak, Nese; Demirci, Selami; Telci, Dilek; Dede, Bulent; Tuzcu, Mehmet; Ozercan, Ibrahim Halil
The field of cancer research has been emerged in recent years for the development of specific drugs to cancer treatment. New agents with the ability to provide efficient treatment by reducing side effects has led to new opportunities for improving agents for cytotoxic therapies. While there are several drugs for colon cancer treatment, researchers are trying to evaluate new agents or combinations of existing ones which can be used efficiently. Schiff bases with a wide range of variety and biological properties including anticancer activity might be used for colon cancer treatment. In the current study, a novel schiff base derivative synthesized by our group was tested in vivo for colon cancer. In a model of azoxymethane (AOM) induced colorectal cancer, chemopreventive properties of schiff base was also analyzed in rats. While AOM induced de novo crypt formation, adenocarcinoma and dysplasia development, schiff base application reduced the number of aberrant crypt foci (ACF), dysplasia or adenocarcinoma. Analysis of the intestinal mucosa showed that peritoneal administration of SB complex not only decreased the protein expression of COX-2, Bcl-2 and NF-kappa B but also enhanced the Bax expression suggesting the apoptotic and anti-proliferative effects for this compound. Our findings showed that SB complex might be used for the colorectal cancer treatment. Further studies are highly warranted to obtain additional insights and identify mode of action for the schiff base.
Oral administration of hesperidin, a citrus flavonone, in rats counteracts the oxidative stress, the inflammatory cytokine production, and the hepatotoxicity induced by the ingestion of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
(John Libbey Eurotext Ltd, 2013) Bentli, Recep; Ciftci, Osman; Cetin, Asli; Unlu, Merve; Basak, Nese; Cay, Mahmut
The objective of the current study was to investigate the protective effects of hesperidin against oxidative stress, altered cytokines levels and histological changes in rats induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Rats were divided randomly into four equal groups (Control, TCDD, hesperidin and TCDD+hesperidin). TCDD and hesperidin were given by gavage, dissolved in corn oil at doses of 2 mu/kg/week and 50 mg/kg/day respectively. The blood and tissue samples were taken from all rats on the 60th day, to be analyzed for the determination of oxidative stress, histological changes and cytokine levels. The results indicated that hesperidin prevented oxidative damage caused by TCDD via decrease lipid peroxidation and increased antioxidant defense systems. It also reversed the histological damage induced by TCDD. Although, TCDD led to a significant increase in TNF-alpha and IL-1 beta levels, hesperidin treatment was able to normalize these values in rats. In conclusion, it was shown that TCDD caused adverse effects as regards cytokine levels, histological alterations and oxidative stress in rats. However, hesperidin treatment mitigated these toxic effects. These results suggest that hesperidin could play a protective role against TCDD toxicity.
The protective effects of chrysin and flunixin meglumine against excess copper in male rats
(Tubitak Scientific & Technological Research Council Turkey, 2018) Seven, Pinar Tatli; Baykalir, Burcu Gul; Seven, Ismail; Ak, Tuba Parlak; Basak, Nese; Gulluoglu, Hakan
Thirty-six Sprague Dawley male rats were housed in individual cages and randomly divided into six groups: control, copper sulfate (Cu; 500 ppm body weight (BW)/day), flunixin meglumine (FM; 2.2 ppm BW/day), chrysin (chrysin; 50 ppm BW/day), copper sulfate + FM (Cu + FM; 500 ppm BW/day of copper sulfate and 2.2 ppm BW/day of FM), and copper sulfate + chrysin (Cu + Chrysin; 500 ppm BW/day of copper sulfate and 50 ppm BW/day of chrysin). Feed intake (FI) in the Cu + Chrysin group significantly increased in comparison with that of the Cu group (P < 0.01). Cu excess significantly increased malondialdehyde, indicating oxidative stress. Chrysin and FM administration significantly decreased malondialdehyde levels and increased the superoxide dismutase and catalase activities in the liver and kidney tissues (P < 0.001). Serum TNF-a levels were significantly lower in the Cu + FM and Cu + Chrysin groups in comparison to the Cu group (P < 0.001). It was seen that FM and chrysin treatments alleviated degeneration, necrosis, and apoptosis in the liver and kidney tissues of the Cu-treated rats. Chrysin appeared to ameliorate the adverse effects on FI and liver and kidney tissues by scavenging where the free radicals are located and increasing the activity of antioxidants.
The Protective Effects of Glycyrrhetinic Acid and Chrysin against Ischemia-Reperfusion Injury in Rat Ovaries
(Hindawi Ltd, 2018) Melekoglu, Rauf; Ciftci, Osman; Eraslan, Sevil; Alan, Saadet; Basak, Nese
Objective. To evaluate the protective effects of glycyrrhetinic acid (GA) and chrysin (CH) on experimental ischemia-reperfusion (I/R) injury in rat ovaries using tissue oxidative stress marker levels, hormone levels, and histopathological scores. Methods. Sixty healthy rats were randomly divided into six equal groups: control, I/R, I/R + CH (50 mg/kg/day), I/R + GA (100 mg/kg/day), CH (50 mg/kg/day), and GA (100 mg/kg/day). Biochemical, hormonal, and histopathological evaluations were performed on blood and tissue samples 14 days after CH and GA treatment. Results. The antioxidant defense system parameters were significantly higher in the ovarian tissues of the I/R + CH and I/R + GA groups than in those of the I/R group. Serum follicle-stimulating hormone levels were significantly reduced, and serum anti-Mullerian hormone levels were significantly increased in rats treated with CH and GA compared with those in the I/R group. Additionally, the histopathological scores of the I/R + CH and I/R + GA groups were significantly improved compared with those of the I/R group. Conclusions. The significant improvements in tissue oxidative stress parameters, serum hormone levels, and histological scores observed in this study indicate that treatment with CH or GA may be a conservative approach to prevent I/R injury in adnexal torsion cases after the ovarian detorsion procedure.
The protective effects of propolis and flunixin meglumine on feed intake, antioxidant status and histological parameters in liver and kidney tissues against excess copper in rats
(Ankara Univ Press, 2018) Tatli Seven, Pinar; Gul Baykalir, Burcu; Parlak Ak, Tuba; Seven, Ismail; Basak, Nese; Yaman, Mine
This experiment was conducted to determine the protective effects of flunixin meglumine and propolis on feed intake, antioxidant status and histological parameters in kidney and liver tissues in rats exposed to excessive copper. In this study individually housed thirty-six male Sprague-Dawley rats were used. Animals were randomly divided into six groups; control, copper sulphate (500 mg/kg BW/day, gavage), flunixin meglumine (2.2 mg/kg BW/day, ip), propolis (100 mg/kg BW/day, gavage), copper sulphate+flunixin meglumine (500 mg/kg BW/day of copper sulphate by gavage and 2.2 mg/kg BW/day of flunixin meglumine, ip) and copper sulphate+propolis (500 mg/kg BW/day of copper sulphate and 100 mg/kg BW/day of propolis by gavage). The study demonstrated that body weight change in the copper sulphate+propolis group significantly ameliorated in comparison with copper group (P < 0.01). Propolis and flunixin meglumine administration significantly decreased MDA levels in the kidney and liver tissues, and serum TNF-alpha levels (P < 0.001). Propolis supplementation to rats who were also treated with copper significantly increased the superoxide dismutase, catalase and reduced glutathione activities (P < 0.001). Flunixin meglumine and propolis treatments decreased the copper-induced degenerative and necrotic changes with the apoptotic cells in the liver and kidney tissues. In conclusion, propolis appeared to ameliorate the adverse effects on feed intake, liver and kidney tissues seeing in the copper treatment and, apparently caused by the copper toxicity, by scavenging the free radicals and increasing activity of antioxidants.
Qual e a eficacia do beta-glucano no tratamento da otite media aguda?
(Assoc Brasileira Otorrinolaringologia & Cirurgia Cervicofacial, 2021) Cetinkaya, Erdem Atalay; Ciftci, Osman; Alan, Saadet; Oztanir, Mustafa Namik; Basak, Nese
Resumo Introducao: Como suplemento, o beta-glucano apresenta varios efeitos terapeuticos gerados pelas celulas imunologicas. Cientificamente aprovado, mostrou ser um modificador de defesa biologica. Objetivo: Investigar os efeitos do beta-glucano nos tratamentos administrados em um modelo de otite media aguda induzida em um modeloanimal. A eficacia foi avaliada imunologica e histologicamente. Metodo: A amostra do estudo foi composta por 35 ratos adultos, divididos aleatoriamente em 5 grupos de 7: grupo 1 (controle), grupo 2 (otite media aguda, sem tratamento), grupo 3 (otite media aguda + antibiotico), grupo 4 (otite media aguda + beta-glucano) e grupo 5 (otite media aguda + beta-glucano + antibiotico). Foram feitas analises dos resultados dos exames histopato-logicos e imunologicos em relacao ao espessamento da membrana timpanica, dano ao epitelio, inflamacao e esclerose. Os niveis sericos de TNF-ci, IL-4, IL-6 e IL-113 foram avaliados em todos os grupos. Resultados: Todos os niveis sericos de citocinas foram significativamente mais baixos nos grupos tratados com beta-glucano e antibioticos em comparacao com o grupo otite media aguda. Diferencas significativas na espessura da membrana timpanica, inflamacao, dano do epitelio e esclerose foram observadas entre os grupos otite media aguda + antibiotico e otite media aguda + beta-glucano. De acordo com esses parametros, os valores no grupo otite media aguda + antibiotico + beta-glucano foram acentuadamente inferiores aos dos demais grupos. Houve uma diferenca significante no grupo otite media aguda + antibiotico + beta-glucano em comparacao ao grupo otite media aguda (p < 0,001). Conclusao: Ambos os tratamentos com antibiotico e com beta-glucano reduziram os sinais de inflamacao da otite media aguda em um modelo de rato com otite media aguda induzida, diminuiram os danos histologicos e os niveis de citocinas. A administracao concomitante de antibiotico e beta-glucano levou a uma reducao significativa na espessura da membrana tim-panica, inflamacao e danos ao epitelio. O tratamento com antibioticos + beta-glucano resultou em maior diminuicao na espessura da membrana timpanica, inflamacao e danos no epitelio do que nos outros grupos. A partir desses resultados, pode-se sugerir que o beta-glucano, em combinacao com antibioticos, pode fornecer uma opcao para o tratamento da otite media aguda. (c) 2020 Associacao Brasileira de Otorrinolaringologia e Cirurgia Cervico-Facial. Publicado por Elsevier Editora Ltda. Este e um artigo Open Access sob uma licenca CC BY (http:// creativecommons.org/licenses/by/4.0/).
A Schiff base derivative for effective treatment of diethylnitrosamine-induced liver cancer in vivo
(Lippincott Williams & Wilkins, 2015) Demirci, Selami; Dogan, Aysegul; Basak, Nese; Telci, Dilek; Dede, Bulent; Orhan, Cemal; Tuzcu, Mehmet
Hepatocellular carcinoma is one of the most prevalent cancers, with a high morbidity rate, even in developed countries. In the present study, the curative effect of the Schiff base (SB) heterodinuclear copper(II) Mn(II) complex on diethylnitrosamine (DEN)-induced liver carcinoma was investigated. Hepatocarcinoma was initiated by an injection of DEN and promoted by phenobarbital (0.05%) in the diet. In addition, the potential nephrotoxicity of SB was evaluated in a cisplatin-induced nephrotoxicity model. Rats were administered the SB complex (1 and 2 mg/kg body weight/day) for 24 weeks, and cancer progression was investigated by macroscopic, histopathological, and western blot examinations. The administration of SB decreased the incidence and the number of hepatic nodules in a dose-dependent manner by regulating inflammation response and the apoptotic pathway. Western blot analyses from the livers of rats treated with SB after DEN induction showed significantly enhanced Bax and caspase-3 levels, with a marked decrease in the levels of Bcl-2, NF-kappa B p65 and cyclooxygenase (COX)-2. Results from the nephrotoxicity study showed that, whereas cisplatin increased serum urea nitrogen and creatinine levels, no increase in serum biochemical parameters was detected in SB-treated animals. Moreover, protein levels of NF-E2-related factor-2 (Nrf2) and heme oxygenase-1 were lower, whereas nuclear factor-kappa B (NF-kappa B p65) and activator protein-1 levels were higher in the kidneys of cisplatin-treated animals compared with that of the SB groups. Therefore, the SB complex could be an alternative chemotherapeutic option for liver cancer treatment once its safety in clinical applications has been examined. Copyright (C) 2015 Wolters Kluwer Health, Inc. All rights reserved.
The therapeutic effects of curcumin and capsaicin against cyclophosphamide side effects on the uterus in rats
(Acta Cirurgica Brasileira, 2018) Yilmaz, Ercan; Melekoglu, Rauf; Ciftci, Osman; Eraslan, Sevil; Cetin, Asli; Basak, Nese
Purpose: To evaluate the impact of systemic cyclophosphamide treatment on the rat uterus and investigate the potential therapeutic effects of natural antioxidant preparations curcumin and capsaicin against cyclophosphamide side effects. Methods: A 40 healthy adult female Wistar albino rats were used in this study. Rats were randomly divided into four groups to determine the effects of curcumin and capsaicin against Cyclophosphamide side effects on the uterus (n=10 in each group); Group 1 was the control group (sham-operated), Group 2 was the cyclophosphamide group, Group 3 was the cyclophosphamide + curcumin (lOOmg/kg) group, and Group 4 was the cyclophosphamide + capsaicin (0.5 mg/kg) group. Results: Increased tissue oxidative stress and histological damage in the rat uterus were demonstrated due to the treatment of systemic cyclophosphamide chemotherapy alone. The level of tissue oxidant and antioxidant markers and histopathological changes were improved by the treatment of curcumin and capsaicin. Conclusion: Cytotoxic effects of natural alkylating chemotherapeutic agents like cyclophosphamide on the uterus can be prevented by curcumin and capsaicin.

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