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Öğe Acute and chronic effects of electroconvulsive treatment on oxidative parameters in schizophrenia patients(Progress in Neuro-Psychopharmacology and Biological Psychiatry, 2011) Kartalcı, Şükrü; Bay Karabulut, Aysun; Özcan, Abdulcemal; Porgalı, Esra; Ünal, SüheylaElectroconvulsive therapy (ECT) is an effective treatment alternative for schizophrenia. Previous studies have already indicated the possible effects of oxidative stress in this disorder. However, there have been no previous studies evaluating the effects of ECT on the oxidative stress in these patients. We therefore aimed to investigate the acute and chronic effects of ECT on serum levels of oxidant and antioxidant molecules in schizophrenia patients (n= 28). The serum MDA and CAT levels of the patients with schizophrenia were higher than that of the controls before ECT (n= 20) but there was no significant difference in the serum NO and GSH levels of the patient groups compared to the controls. We found that the NO levels of the patients were higher than the controls in the group experiencing their first episode but not in the chronic group. There was a significant clinical improvement in the patients in terms of BPRS, SANS and SAPS reduction after the 9th ECT, but not the 1st ECT. Serum MDA levels were significantly reduced compared to the baseline after the 9th ECT session although there was no significant difference after the 1st session. Separate evaluation of the patient groups revealed that the significant MDA decrease following ECT was in the patients experiencing their first episode and not in the chronic group. No significant difference was noted in the serum levels of other oxidant and antioxidant molecules after either the 1st or 9th ECT session. These results suggest that ECT does not produce any negative effect on oxidative stress in patients with schizophrenia.Öğe Changing of antioxidant enzyme activity on the biliary obstructed rats treated with resveratrol(Dtsch Tierarztl Wochenschr, 2007) Çenesiz, Sena; Yarım, G. F.; Bay Karabulut, Aysun; Ara, CengizThe aim of this study was to evaluate the role of antioxidant enzyme activity and nitric oxide levels induced by 28 day biliary obstruction in the rat. A total of 21 young Swiss albino rats were divided in to 3 groups. Bile duct ligations, bile duct ligations plus resveratrol, sham operated. Bile duct ligations plus resveratrol group received 10 mg/kg dose of resveratrol intraperitonealy once daily throughout for 28 days. Liver damage and cholestasis were determined by biochemical examination. SOD, CAT and GSH-PX activity decreased in BDL group compared with shame opareted groups (p < 0.001). NO levels increased in BDL groups compared with shame opareted groups (p < 0.001). SOD, CAT and GSH-PX activity was found higher in BDL+resveratrol treated groups than BDL groups (p < 0.001). In addition this NO levels decreased in BDL+resveratrol treated groups than BDL groups (p < 0.001). In conclusion, it is thought that resveratrol may be used as a protective agent in biliary obstructions; however, further clinical and experimental studies are needed to verify its antioxidative and hepatoprotective effects.Öğe The effect of melatonin against FK506 induced renal oxidative stress in rats(Surgical Innovation, 2011) Ara, Cengiz; Dirican, Abuzer; Ünal, Bülent; Bay Karabulut, Aysun; Pişkin, TurgutBackground: Nephrotoxicity is an important side effect of FK506 and oxidative stress has been considered as one of the possible mechanisms. The present investigation examines the ability of melatonin to protect against FK506-induced renal oxidative stress. Methods: Thirty rats were divided into 3 groups (n = 10 each group). Group A was the sham group. Group B received 14 days FK506 (5 mg/kg/d, intraperitoneally [i.p.]) and group C received FK506 (5 mg/kg/d, i.p.) together with melatonin (4 mg/kg, i.p.) for 14 days. Kidney tissues were harvested to determine the tissue levels of malondialdehyde (MDA), total nitrite and nitric oxide (NO), tumor necrosis factor-a (TNF-a), and interleukin-6 (IL-6). Results: In group C, the levels of TNF-a, IL-6, and NO were lower than in the group B (P < .01, P < .03, and P < .04, respectively) and although MDA levels were lower than in group B, the differences were not statistically significant (P > .05). Conclusion: These results suggest that melatonin has protective effect against FK506-induced renal oxidative stress.Öğe The effect of resveratrol on surgery induced peritoneal adhesions in an experimental model(Cell Biochemistry and Function, 2007) Söğütlü, Gökhan; Bay Karabulut, Aysun; Ara, Cengiz; Cinpolat, Özgür; Işık, Burak; Pişkin, Turgut; Çelik, ÖnderAdhesion formation is a common cause of complications following surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model. Twenty one Wistar-Albino rats weighing 200–250 g were assigned to three groups, of 7 rats each. After a midline laparotomy was performed, a 1 cm area of the ceacum was abraded in two of the groups. They were then given either resveratrol (Group 1), or saline (Group 2) intraperitoneally. Group 3 rats (sham operation) received no treatment, without the serosal damage. On the 14th day, the rats were killed and the adhesion score was determined according to Mazuji’s adhesion grade scale. The tissue levels of malondialdehyde (MDA), nitric oxide (NO), and reduced glutathione (GSH) were measured. The mean Mazuji’s adhesion grade in the resveratrol group was 1.0 0.0, in the saline group 2.57 1.51, and zero in the sham operated group (p < 0.05 between the resveratrol group and saline group comparison). The levels of MDA and NO in the resveratrol group were significantly lower than those of the saline group (p < 0.001). The level of GSH in the resveratrol group was significantly higher than in the saline and sham operated groups (p < 0.001 and p < 0.001, respectively). Introduction of resveratrol into the peritoneal cavity at the time of surgery reduced adhesion formation effectively in this model. Resveratrol probably acts through reduction of lipid peroxidation products.Öğe Evaluation of Oxidative Stress Status and Adenosine Deaminase Activity in Hyperthyroid and Hypothyroid Patients(2014) Kıran, Tuğba Raika; Bay Karabulut, Aysun; Şahin, İbrahim; Çolak, CemilAbstract: A few prospective studies evaluated the oxidative stress variables and adenosine deaminase activity in patients with hyperthyroidism and hypothyroidism. Thus; we aimed to investigate oxidative stress variables and adenosine deaminase activity in patients with hyperthyroidism and hypothyroidism. Study populations consisted of 20 patients with hyperthyroidism, 20 patients with hypothyroidism and 20 age matched healthy volunteers as control group. Plasma nitric oxide, malondialdehyde levels and glutathione, adenosine deaminase activities were measured in all subjects. plasma nitric oxide and adenosine deaminase levels were higher in patients with hyperthyroidism, but lower in patients with hypothyroidism compared to those of controls (P<0.05). Plasma nitric oxide levels were higher in patients hypothyroidism compared to the control group (P<0.05). Glutathione levels were lower in both patients with hypo and hyperthyroidism compared to the control group (P<0.05). Our results suggest that both patients with hyperthyroidism and hypothyroidism may have effect on oxidative stress status and adenosine deaminase activity.Öğe Glutathione and malondialdehyde levels in patients with hydatid cyst(Saudi medical journal, 2008) Atambay, Metin; Karaman, Ülkü; Kıran, Tuğba Raika; Çolak, Cemil; Bay Karabulut, Aysun; Çelik, Tuncay; Daldal, NilgünÖğe Malondialdehyde glutathione and nitric oxide levels in patients withenterobius vermicularis infection(Mikrobiyoloji Bulteni, 2010) Kıran, Tuğba Raika; Karaman, Ülkü; Çolak, Cemil; Bay Karabulut, Aysun; Ülfet Daldal, NilgünThe aim of this study was to investigate the levels of malondialdehyde (MDA) and nitric oxide (NO) which are indicators of oxidative stress and also the level of glutathione (GSH) which is an antioxidant molecule, in patients with Enterobius vermicularis infection. A total of 41 patients (mean age: 36.4 years; 31 were male) and 40 healthy controls (mean age: 40.2 years; 28 were male) were included to the study. None of the patients and controls have had history of hormone/steroid drug use, smoking and alcoholism. The mean level of GSH in patient and control groups were found 1.17 +/- 0.02 micromol/l and 2.49 +/- 0.10 micromol/l; MDA 26.97 +/- 2.06 micromol/l and 19.47 +/- 2.25 micromol/l; NO 20.74 +/- 0.60 micromol/dl and 17.83 +/- 0.50 micromol/dl, respectively. The mean GSH level in patient group was statistically significantly lower (p < 0.05) than controls, while the mean MDA and NO levels were statistically significantly higher (p < 0.05). These results indicated that the consumption of GSH was increased due to the severity of oxidative stress in patients infected with E. vermicularis. Detailed experimental and clinical studies are required to enlighten the relation of GSH in the pathogenesis of E. vermicularis infection. Since oxidative stress is increased during enterobiosis, the use of antioxidant agents (e.g. vitamins C and E) for the supportive treatment deserves evaluation.Öğe Melatonin ameliorates tacrolimus FK 506 s induced immunosupressive effect in rat liver(Transplant Proc, 2009) Ara, Cengiz; Bay Karabulut, AysunTacrolimus (FK-506) is a powerful immunosuppressive agent that modulates neutrophil infiltration during inflammation. In this study, we sought to investigate the effects of melatonin on malondialdehyde (MDA), nitric oxide (NO), interleukin (IL)-6, and tumor necrosis factor-alpha (TNF-alpha) to oppose the negative effects of the immunosupressant FK-506. Group A was sham; group B, tacrolimus (1 mg/kg/d subcutaneously); and Group C received tacrolimus (1 mg/kg/d plus melatonin). All tissues underwent histopathologic examination. The MDA level in group B increased 53% compared with the sham group (P .001); in group C, the MDA level decreased 16% compared with group B (P .05). While TNF-alpha in group B increased 68.8% compared with the sham group (P .001) and in group C it decreased 63.5% compared with the sham group. The IL-6 level in group B increased 81%; in group C, it decreased 13% compared with group B. NO levels in group B increased 48% compared with the sham group and increased by 15% in group B compared with group C. Thus melatonin may serve as a protective agent against the side effects of tacrolimus.Öğe Protective effect of aminoguanidine against oxidative stress in an experimental peritoneal adhesion model in rats(Cell Biochem Funct., 2006) Ara, Cengiz; Bay Karabulut, Aysun; Kırımlıoğlu, Saime Hale; Yılmaz, Mehmet; Kırımlıoğlu, Vedat; Yılmaz, SezaiPostoperative intraperitoneal adhesion formation is a major cause of intestinal obstruction, pain and infertility. This experimental study was designed to evaluate the degree of adhesion formation and peritoneal tissue levels of malondialdehyde (MDA), reduced glutathione (GSH) and total nitrite and nitrate (NO) and the effect of aminoguanidine (AG) on these metabolite values after postoperative intraperitoneal adhesion formation in rats. A total of 21 adult male Wistar albino rats were randomly divided into three groups. Control rats were untreated; the AG group received AG 200 mg kg 1 i.p. for 10 consecutive days intraperitoneally after surgery. The sham group was given 0.9% NaCl. The rats were killed on postoperative day 10. The peritoneal tissues were harvested to determine the tissue levels of MDA, GSH, and NO activity. For light microscopic evaluation, the cecum was removed. Adhesion formation scores in the AG group were significantly lower than those of the control and sham groups ( p < 0.017, p < 0.026 respectively). In the AG-treated rats, tissue levels of MDA and NO were significantly lower than in the control group ( p < 0.017). The levels of GSH in aminoguanidine-treated rats were significantly higher than those of the control group ( p < 0.01). The severity of the inflammation was more prominent in the control group compared with the AG-injected rats. The results demonstrate that in this experimental model, intraperitoneal administration of aminoguanidine decreases the incidence and extent of peritoneal adhesions and causes a decrease in MDA and NO and an increase in GSH values.Öğe Protective effect of melatonin against oxidative stress on adhesion formation in the rat cecum and uterine horn model(Life Sci., 2005) Ara, Cengiz; Kırımlıoğlu, Saime Hale; Bay Karabulut, Aysun; Çoban, Sacid Abdussamet; Hasçalık, Şeyma; Çelik, Önder; Yılmaz, Sezai; Kırımlıoğlu, VedatThis experimental study was designed to evaluate the degree of adhesion formation and peritoneal tissue levels of malondialdehyde (MDA), reduced glutathione (GSH) and nitric oxide (NO) and the effect of melatonin on these metabolites in a postoperative intraperitoneal adhesion formation model in rats. Thirty adult female Wistar albino rats were subjected to standardized lesions by cecal and uterine horn abrasion and were randomly divided into three groups. Control rats were treated with 5% ethanol. Melatonin treated rats received 4 mg/kg melatonin before closure and for 10 consecutive days intraperitoneally after surgery. Rats in the sham operation group underwent a surgical procedure similar to the other groups however the peritoneal abrasion was not performed. On postoperative day 10 relaparatomy was performed. After the assessment of the adhesions, the rats in each group were sacrificed and peritoneal tissues were harvested to determine the tissue levels of MDA, GSH and NO activity. Adhesion formation scores in the melatonin group were significantly lower than that of control and sham group ( p b0.01 and p b0.02, respectively). Tissue levels of MDA and NO were significantly lower in the melatonin treated rats when compared with control and sham groups. The levels of GSH in the melatonin treated rats were significantly higher than those of control and sham groups ( p b0.01). The results demonstrate that in this experimental model, intraperitoneal administration of melatonin decreases the extent of peritoneal adhesions and causes a decrease in MDA and NO and an increase in GSH levels.Öğe Protective effect of resveratrol against renal oxidative stress in cholestasis(Ren Fail., 2005) Ara, Cengiz; Bay Karabulut, Aysun; Çoban, Sacit; Uğraş, Murat; Kırımlıoğlu, Vedat; Yılmaz, SezaiThis experimental study was designed to evaluate histological changes of the kidney and renal tissue levels of malondialdehyde (MDA), reduced glutathione (GSH), and nitric oxide (NO) and the effect of resveratrol on these metabolites after bile duct ligation in rats. Methods. Secondary biliary cirrhosis was induced by bile duct ligation for 28 days. Swiss albino rats were divided into three groups. Group 1: Sham (n = 7), Group 2: Bile duct ligation (n = 7), Group 3: Bile duct ligation plus resveratrol (n = 7). Bile duct ligation (BDL) plus resveratrol group received 10 mgr/kg dose of resveratrol intraperitoneally daily throughout 28 days. Kidney tissues were harvested to determine the tissue levels of MDA, GSH, and NO activity. Liver and kidney tissues were removed for light microscopic evaluation. Results. Cholestasis was determined by biochemical and pathologic examination. In the resveratrol-treated rats, levels of MDA were significantly lower than those of the BDL group (p < 0.04). The levels of GSH in the resveratrol-treated rats were significantly higher than those in the BDL group (p < 0.01). The levels of NO in the resveratrol group were significantly lower than those in the BDL group (p < 0.01). Conclusion. The present study demonstrates that intraperitoneal administration of resveratrol in bile duct ligated rats maintains antioxidant defenses and reduces kidney oxidative damage. This effect of resveratrol may be useful in the preservation of renal oxidative stress in cholestasis.Öğe Protective effects of resveratrol on spleen and ileum in rats subjected to ischemia reperfusion(Transplantation Proceedings, 2006) Bay Karabulut, Aysun; Kırımlıoğlu, Vedat; Kırımlıoğlu, Saime Hale; Yılmaz, Sezai; Işık, Burak; Işıkgil, O.Resveratrol is as an antioxidant with free radical-scavenging activity and finds its clinical application in the prevention of postischemic tissue injury following solid organ transplantation. This study investigates the effect of Resveratrol on spleen and ileum tissues subjected to hepatic ischemia-reperfusion (I/R) in rats. Twenty-four rats were recruited in the study as follows: group A: I/R (n 8), group B: I/R Resveratrol (n 8), and group C: sham operation (n 8). After intraperitonealy pretreatment of eight rats with resveratrol (15 mg/kg/d) for 5 days, 16 rats were subjected to 45 minutes of hepatic ischemia followed by 30 minutes reperfusion period. Resveratrol was given 15 minutes prior to ischemia and just before the reperfusion in rats. After reperfusion period all rats were sacrified. Spleen and ileum tissues were examined spectrophotometrically to measure malondialdehyde (MDA), glutathione (GSH), and total nitrite, nitrate as an end product of nitric oxide (NO) levels. Concerning the spleen, statistically significant decrease of GSH and increase of MDA and NO levels were found group A when compared to groups B and C (P .040, P .004, and P .001 group A vs group B; P .05, P .003, and P .001 group A vs group C, respectively). Parallel results were obtained in ileum. A statistically significant decrease in GSH and an increase in MDA and NO levels in group A in respect to group B and group C was obtained (P .048, P .034, and P .001 group A vs group B; P .004, P .001, and P .003 group A vs group C, respectively). The result of this study shows that resveratrol has a protective effect on spleen and ileal mitochondrial oxidative stress in rats subjected to I/R.Öğe The Relationship between Obesity and Oxidative Stress and Cardiac Markers(2015) Unver Baki, Pınar; Bay Karabulut, Aysun; Cikim Sertkaya, Ayse; Kıran, Tuğba Raika; Yağmur, JülideAbstract: Obesity is one of the most even leading issue for the last era but however the reasons for this epidemic could not been explained clearly yet. We aimed to investigate the relationship between obesity, and myoglobin and homocysteine in means of cardiac markers and the levels of nitric oxide in means of oxidative stress and leptin. Study populations consisted of 30 patients with obesity and 30 healthy volunteers as control group. Serum nitric oxide, homocysteine, leptin and myoglobin were higher in obese individuals compared to controls. Nitric oxide level was related to myoglobin levels and seems to alter the myoglobin concentration. A negative and strong correlation was defined for myoglobin with both gender and age. There was a positive correlation between Body Mass Index and homocysteine. This is one of the studies investigating the relationship between nitric oxide as an oxidation marker homocysteine and myoglobin as cardiac markers, and leptin with obesity to lighten the complex relationship for the issue.Öğe Resveratrol a red wine constituent polyphenol protects gastric tissue against the oxidative stress in cholestatic rats(Digestive Diseases and Sciences, 2006) Kırımlıoğlu, Vedat; Ara, Cengiz; Yılmaz, Mehmet; Özgör, Dinçer; Işık, Burak; Söğütlü, Gökhan; Kırımlıoğlu, Saime Hale; Bay Karabulut, Aysun; Yılmaz, Sezai; Kayaalp, Cüneyt; Yoloğlu, SaimThis experimental study was designed to determine the effects of resveratrol on the level of malondialdehyde (MDA), reduced glutathione (GSH), and nitric oxide (NO) in gastric tissue after bile duct ligation (BDL). Swiss albino rats were divided into three groups: Group 1, sham (n = 7); Group 2, BDL (BDL only group; n = 7); and Group 3, BDL plus resveratrol (n = 7). Animals in the resveratrol group were treated with 10 mg/kg resveratrol (i.p.) once a day throughout 28 days. In the resveratrol group, levels of MDA and NO in gastric tissue were significantly lower than in the BDL-only group (P < 0.001). The level of GSH in the resveratrol group was significantly higher than in the BDL-only group (P < 0.001). The present study demonstrates that intraperitoneal administration of resveratrol maintains antioxidant defenses and reduces oxidative gastric damage. This effect of resveratrol may be useful to preserve gastric tissue under oxidative stress due to cholestasis.Öğe The role of oxidative stress in postoperative delirium(General Hospital Psychiatry, 2006) Karlıdağ, Rıfat; Ünal, Süheyla; Sezer, Özlem; Bay Karabulut, Aysun; Battaloğlu, Bektaş; But, Abdulkadir; Özcan, AbdulcemalAim: This study aimed to determine a marker that predicts delirium using preoperative oxidative processes in patients undergoing cardiopulmonary bypass surgery. Method: Twelve of the 50 patients included in the study showed signs of delirium during postoperative follow-up. The Delirium Rating Scale was used in patients with delirium according to DSM-IV-TR in the postoperative period. Venous blood samples were obtained from the patients the day before and the day after the surgery to determine plasma antioxidant enzyme levels. Results: While there were no differences in preoperative superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) levels in both groups, catalase (CAT) levels were significantly lower in the delirium group. Postoperative SOD and MDA levels were also higher in the delirium group, while the GSH-Px levels were found to be lower when compared with those during the preoperative period. In the nondelirium group, the postoperative MDA and GSH-Px levels were found to be lower than preoperative levels, and postoperative SOD levels were found to be higher than preoperative levels. CAT levels were lower in the delirium group when the pre- and postoperative levels were compared in both groups. The postoperative levels of SOD, GSH-Px and CAT in the nondelirium group and MDA in the delirium group were significantly higher than preoperative levels. Conclusion: Patients with low preoperative CAT levels appeared to be more susceptible to delirium than patients with higher CAT levels.Öğe Single center analysis of the first 304 living donor liver transplantations in 3 years(Hepato-gastroenterology, 2013) Yılmaz, Sezai; Kayaalp, Cüneyt; Ara, Cengiz; Yılmaz, Mehmet; Işık, Burak; Bay Karabulut, Aysun; Aydın, Cemalettin; Özgör, Dinçer; Dirican, AbuzerLiving donor liver transplantations (LDLT) has been established as an excellent treatment method for patients with end-stage liver disease and has achieved exponential growth, especially in the countries that have the donation problem. Between April 2007 and April 2010, we performed LDLT in 289 patients. Fifteen of the cases required re-transplantations. In the present study, these 304 consecutive LDLTs were evaluated to determine both donor and recipient outcomes. Methodology: Complication rates and survival data of the recipients and donors of 304 LDLT cases were analyzed. Results: All donors were alive and well. Overall complication rate was 27% (83 donors). These complications included bile leakage in 2%, intraabdominal bleeding in 2%, chylous peritonitis in 0.6%, hepatic venous obstruction due to not performing falsiformepexia in 0.3%, wound infection in 11%, incisional henia in 2%, and pulmonar complications (atelectasia, pneumonia) in 8%. The recipient complication rate was 51% in early postoperative period. The most frequent complication was infections. Five patients died due to aggressive infections. In the long term there were 57 biliary stricture cases. Five patients had chronic bile fistula. Hepaticojejunostomies were performed in 13 patients. Endoscopic stents were placed in 20 cases. Twenty-four patients were managed by percutaneous biliary catheter. Chronic and acute rejection attacks developed in 7 and 103 patients respectively. Hepatic artery thrombosis developed in 25 patients (8%). The mean follow-up was 19 months. One, two and three year survival rates were 82%, 79% and 75% respectively. In hospital mortality rate was 16%. There were a total of 74 (25%) recipient mortalities along follow up period due to 15 vascular complications, 39 septic complications, 9 liver dysfunctions, 6 chronic rejections and 5 different causes. Conclusions: More than 150 liver tranplantations per year in a single center is a challenge in Turkey, where there is a shortage of deceased donor grafts. LDLT is a safe procedure for the donors and an effective therapy for the patients with end-stage liver diseases. We believe that with accumulation of experience in surgery and clinical management, better outcomes of LDLT can be expected.
