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    Exploiting ionisable nature of PEtOx-co-PEI to prepare pH sensitive, doxorubicin-loaded micelles
    (Taylor & Francis Ltd, 2020) Ozturk, Naile; Kara, Asli; Gulyuz, Sevgi; Ozkose, Umut Ugur; Tasdelen, Mehmet Atilla; Bozkir, Asuman; Yilmaz, Ozgur
    Aims This study was conducted to evaluate block copolymers containing two different poly(ethyleneimine) (PEI) amounts, as new pH-sensitive micellar delivery systems for doxorubicin. Methods Micelles were prepared with block copolymers consisting of poly(2-ethyl-2-oxazoline)-co-poly(ethyleneimine) (PEtOx-co-PEI) and poly(epsilon-caprolactone) (PCL) as hydrophilic and hydrophobic blocks, respectively. Doxorubicin loading, micelle size, pH-dependent drug release, and in vitro cytotoxicity on MCF-7 cells were investigated. Results The average size of drug-loaded micelles was under 100 nm and drug loading was between 10.7% and 48.3% (w/w). pH-sensitive drug release was more pronounced (84.7% and 68.9% (w/w) of drug was released at pH 5.0 and pH 7.4, respectively) for the micelles of the copolymer with the lowest PEI amount. The cell viability of doxorubicin-loaded micelles which were prepared by the copolymer with the lowest PEI amount was 28-33% at 72 h. Conclusions PEtOx-co-PEI-b-PCL micelles of this copolymer were found to be stable and effective pH-sensitive nano-sized carriers for doxorubicin delivery.
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    Microfluidics-Based Nanoparticle Formulations: Preparation and Evaluation of Protein Delivery Systems
    (Wiley, 2025) Bezelya, Aysenur; Kucukturkmen, Berrin; Boncu, Tugba Eren; Bozkir, Asuman
    Polymeric nanoparticles have attracted significant attention due to their potential in drug delivery, material science, and chemistry. In general, the targeted activities of nanoparticles (NPs) are affected by their size and morphology. Microfluidic methods offer precise control over nanoparticle properties, providing better reproducibility and uniformity. This study investigates the effects of microfluidic method parameters on the physicochemical properties and protein delivery potential of synthesized poly(lactic-co-glycolic acid) (PLGA) nanoparticles. The size of the nanoparticles was precisely tuned by varying the flow rate ratios (FRR), total flow rate (TFR), polymer, protein, and surfactant concentrations. Proteins with various molecular weights, including bovine serum albumin (BSA), lysozyme, and aprotinin, were effectively encapsulated, and their drug release kinetics and structural integrity were investigated. By simultaneously evaluating three structurally distinct model proteins within a single microfluidic system, this study provides a comprehensive insight into the role of protein size and charge on nanoparticle formation and release behavior for the first time. This research contributes to the advancement of nanoparticle formulation strategies using microfluidic technology. Microfluidic systems hold great potential for rapid, easy, effective, low-cost, and high-yield NP production.