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Öğe Assessment of myoelectrical signal parameters in estrogen, progesterone, and human chorionic gonadotropin administered in nonpregnant rat myometrium after ovariectomy(Elsevier Science Inc, 2008) Celik, Onder; Hascalik, Seyma; Tagluk, M. Emin; Elter, Koray; Parlakpinar, Hakan; Acet, AhmetObjective: To investigate the correlation of myoelectrical signals with spontaneous contractile events and physiological states in the nonisolated uterine horn of rats. Design: In vivo uterine myoelectrical activity recording study. Setting: Animal and pharmacology laboratory at Inonu University. Animal(s): Thirty-six female Wistar albino rats. Intervention(s): Six animals were not castrated and served as a sham-operated control group; the other 30 were ovariectomized (OVX) and put into groups: unbiased OVX subjects, estrogen (E)-biased OVX subjects, P-biased OVX subjects, E-plus-P-biased OVX subjects, and hCG-biased OVX subjects. An MP100 A-CE was used for data acquisition, and a personal computer was used for processing. Main Outcome Measure(s): Besides the temporal, spectral, and joint time-frequency (spectrotemporal) analysis, some quantitative measures such as standard deviation and mark to space power I ratios of myoelectrical signals were measured. Result(s): Progesterone, E, and hCG administration down-regulated the power and contraction frequency of the uterine electrical signal. The spectral concentrations that occurred around the 0.9, 0.35, and 0.7 Hz frequency ranges may be distinguishing characteristics for P, E, and hCG, respectively. Conclusion(S): Based on the obtained results, uterine contractions change with ovariectomy and administration of hormones. Progesterone, E, and hCG particularly prolong the quiescent periods of the uterus by reducing the frequency of uterine contractions as well as the power of the myoelectrical activity. Individual or combined use of R. or hCG might favor quiescence of the uterine muscle and the maintenance of pregnancy.Öğe Combating endometriosis by blocking proteasome and nuclear factor-?B pathways(Oxford Univ Press, 2008) Celik, Onder; Hascalik, Seyma; Elter, Koray; Tagluk, M. E.; Gurates, Bilgin; Aydin, N. E.BACKGROUND: The objective of this study is to investigate the effect of pyrrolidine dithiocarbamate [PDTC; a nuclear factor-kappaB (NF-kappa B) inhibitor] and bortezomib (Velcade; a proteasome inhibitor) on the development of experimental endometriotic implants in rats. METHODS: Endometriosis was surgically induced in 30 rats using the method of Vernon and Wilson. Three weeks later the viability and volume of the implants were recorded and classified. Afterwards, rats were put into three groups with equal numbers. The groups were labelled as the control, the PDTC and the bortezomib groups. Seven days after treatment, a third laparotomy was done and the volume of implants was measured again. The animals were then sacrificed, and the implants were stained with Ki67, proliferating cell nuclear antigen (PCNA), CD34, CD31 and Masson's trichrome histochemical staining. RESULTS: In 80% of the implanted rats, vesicles at the suture region were observed, and the rats graded according to average vesicle diameter (D) as: Grade 1 (no vesicle, 20% of rats), Grade 2 (D < 2 mm, 33.3% of rats), Grade 3 (2 mm < D > 4.5 mm, 26.7% of rats) and Grade 4 (D > 4.5 mm, 20% of rats). After treatment with PDTC or bortezomib, these percentages were decreased for Grades 3 and 4, and increased in Grade 1. The post-treatment implant volumes were decreased in the PDTC and bortezomib groups (P < 0.002 and P < 0.001), and slightly increased in the control group (P = 0.279). In the PDTC and bortezomib groups, CD34, CD31, PCNA and Ki67 expression levels were similar but were significantly reduced compared with the control group. CONCLUSIONS: PDTC and bortezomib may represent a novel therapeutic strategy for treatment of endometriosis.Öğe Novel noninvasive detection method for endometriosis: research and development of scintigraphic survey on endometrial implants in rats(Elsevier Science Inc, 2008) Hascalik, Seyma; Celik, Onder; Kekilli, Ersoy; Elter, Koray; Karakas, Hakki Muammer; Aydin, Nasuhi EnginIn this experimental study on endometriosis, the majority of the implants were successfully detected with technetium-(Tc-99m) labeled red blood cell scintigraphy.Öğe Spectrotemporal changes in electrical activity of myometrium due to recombinant follicle-stimulating hormone preparations follitropin alfa and beta(Elsevier Science Inc, 2008) Celik, Onder; Tagluk, M. Emin; Hascalik, Seyma; Elter, Koray; Celik, Nilufer; Aydin, Nasuhi EnginObjective: To investigate the effects of follitropin alfa and beta on the myoelectrical activity of rat myometrium using signal-processing techniques. Design: Prospective, placebo-controlled study. Setting: Animal and pharmacology laboratory at Inonu University. Animal(S): Forty-five female Wistar albino rats. Intervention(s): Thirty of 45 animals involved in the experiment were registered as the superovulation group. After two successive normal estrous cycles, these animals were put into three equal subgroups. Group 1 was the control; animals were given 0.9% saline. Groups 2 and 3 were treated with follitropin alfa (Gonal-f) and follitropin beta (Puregon), respectively. The other 15 animals were ovariectomized (OVX) and subjected to the same protocol. The uterine myoelectrical signals were recorded and analyzed using a Matlab environment. Main Outcome Measure(s): Power/second, variance, and the effects of recombinant human follicle-stimulating hormone (FSH) on myoelectrical signals were assessed through temporal, spectral, and joint time-frequency analysis. The uterine endometrium and ovarian morphology were also assessed concerning primary follicles, antral follicles, and corpora lutea. Result(S): The power and some characteristic spectral components of myoelectrical signal were reduced with the administration of follitropin alfa and beta. No statistically significant difference was detected between endometrial and ovarian histology of the rats treated with these follitropins. Conclusion(s): Uterine myoelectrical signals change with administration of recombinant human FSH preparations. Follitropin beta and, more precisely, follitropin alfa suppress the spectral components and power of the myoelectrical signals, which provides uterine quiescence.Öğe Use of porcine small intestinal submucosa to reconstruct an ovarian defect(Elsevier Ireland Ltd, 2009) Celik, Onder; Esrefoglu, Mukaddes; Hascalik, Seyma; Gul, Mehmet; Tagluk, M. Emin; Elter, Koray; Aydin, EnginObjective: To investigate the feasibility of using porcine small intestinal submucosa (SIS) as a scaffold for repairing ovarian defects. Method: Fourteen female New Zealand rabbits undergoing ovarian resection were randomly allocated to 2 equal groups. The unilateral ovarian defects were, repaired with SIS in group I animals and without SIS in group 2 animals (control). The volumes of the ovaries were calculated and the severity of adhesions was assessed in I animal from each group each month. The ovaries were removed and examined under a microscope. Results: The volumes of the SIS-grafted ovaries were larger than those of the operated ovaries of the control animals (P<0.05). The SIS-grafted ovaries had a lower adhesion score than the operated ovaries of the control group (P<0.001). SIS grafts showed hemorrhage and leukocyte infiltration until the 4th week after surgery, but the ovarian tissue appeared to be well organized from the 12th to the 16th week. At the 28th week, primordial follicles were scattered in the SIS graft. Conclusion: SIS graft could be used for repairing the ovary after surgery. (C) 2009 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.
