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Öğe Assessment of heavy metal accumulation and essential nutrients in fruits: implications for food safety and environmental sustainability(Springer, 2025) Yumusakbas, Hatice; Ugur, Yilmaz; Maras, Zeynep; Buyuksoylu, Semih; Erdogan, SelimThis study investigates the total concentrations of essential and potentially toxic elements in cherries (Prunus avium), strawberries (Fragaria x ananassa), and grapes (Vitis vinifera) to assess food safety and environmental sustainability. A total of 550 fruit samples and corresponding leaf and soil samples were collected from cherry, strawberry, and grape plants across 60 individuals, with 1 kg of soil sampled per plant. Elemental concentrations were determined using Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The results indicated that potassium (K) was the dominant macro element in strawberries (3242.08 mg/kg), while calcium (Ca) exhibited the highest concentration in grapes (4748.0 mg/kg). Iron (Fe) concentrations were notably high in grape soils (170.85 mg/kg), besides cadmium (Cd) concentrations exceeded permissible regulatory limits in all samples, suggesting contamination from agricultural sources. Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) revealed distinct elemental patterns between fruit, leaf, and soil compartments. These findings highlight the necessity of continuous monitoring and regulatory enforcement to mitigate heavy metal exposure in agricultural products.Öğe Changes in melatonin, cortisol, and body temperature, and the relationship between endogenousmelatonin levels and analgesia consumption in patients undergoing bariatric surgery(Sprınger, 233 sprıng st, new york, ny 10013 usa, 2018) Altunkaya, Neslihan; Erdogan, Mehmet Ali; Ozgul, Ulku; Sanli, Mukadder; Ucar, Muharrem; Ozhan, Onural; Sumer, Fatih; Erdogan, Selim; Colak, Cemil; Durmus, MahmutBackground Melatonin has analgesic, anti-inflammatory, sedative, and anxiolytic properties. However, the relationship between endogenous melatonin levels and postoperative analgesic requirements has not been well elucidated in patients undergoing bariatric surgery. We studied endogenous melatonin levels, cortisol levels, body temperatures, and the relationship between the level of endogenous melatonin and postoperative morphine consumption. Methods The trial was conducted among 30 patients who were scheduled for laparoscopic bariatric surgery. Their ages were between 18 and 65 years and their BMIs were above 40 kg/m(2). Secretion of melatonin, cortisol, and body temperature was monitored before the anesthetic induction, at 2 h intraoperatively, and at 2, 6, 10, (2:00 A.M.) and 24 h postoperatively. For each patient, morphine consumption was assessed at postoperative visits. The primary outcomes were to measure endogenous melatonin levels and to examine the relationship between these levels and morphine consumption. The secondary outcome was to observe the changes in cortisol and body temperature. Results There was a significant decrease in melatonin levels when preoperative melatonin levels were compared with intraoperative and all postoperative follow-up periods (p < 0.05). When the correlation between plasma melatonin levels and the postoperative morphine consumption of the patients was inspected, there was a significant correlation in all of the follow-up periods (p < 0.05). When preoperative cortisol levels were compared with intraoperative and postoperative cortisol levels, there was a significant difference in the follow-up periods, except two periods (p < 0.05). Body temperatures were similar in all measurement periods. Conclusions Endogenous melatonin secretion was significantly decreased in the intraoperative and postoperative periods. Furthermore, there was a significant inverse correlation between changes in endogenous melatonin levels and morphine consumption.Öğe Chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of Mediterranean Pinus brutia and Pinus pinea resin essential oils(Chinese Journal Natural Medicines, 2014) Ulukanli, Zeynep; Karaborklu, Salih; Bozok, Fuat; Ates, Burhan; Erdogan, Selim; Cenet, Menderes; Karaaslan, Merve GoksinEssential oils of the resins of Pinus brutia and Pinus pinea were evaluated for their biological potential. Essential oils were characterized using GC-MS and GC/FID. In vitro antimicrobial, phytotoxic, antioxidant, and insecticidal activities were carried out using the direct contact and the fumigant assays, respectively. The chemical profile of the essential oils of the resins of P. pinea and P. brutia included mainly a-pinene (21.39% and 25.40%), beta-pinene (9.68% and 9.69%), and caryophyllene (9.12% and 4.81%). The essential oils of P. pinea and P. brutia exerted notable antimicrobial activities on Micrococcus luteus and Bacillus subtilis, insecticidal activities on Ephestia kuehniella eggs, phytotoxic activities on Lactuca sativa, Lepidium sativum, and Portulaca oleracea, as well as antioxidant potential. Indications of the biological activities of the essential oils suggest their use in the formulation of ecofriendly and biocompatible pharmaceuticals.Öğe Comparative Assessment of Kernel Chemical Properties from Six Sweet Cherry Cultivars at Four Ripening Stages(Springer, 2025) Nalcaci, Sultan; Onal, Yunus; Zengin, Rukiye; Toptas, Yeliz; Erdogan, SelimFruits are industrially processed, producing a significant amount of waste and by-products including peels, stones and seeds. These significant agri-food wastes are considered as a source of high-value-added compounds for use in the nutraceutical, chemical, cosmetic and pharmaceutical industries. The aim of this study was to define the profiles and evaluate the content of fatty acids in kernel oils obtained from fruit seeds of six different cultivars of sweet cherries ('Sweet Heart', '0900 Ziraat', 'Hardy Giant', 'Dalbast & imath;', 'Starks Gold', 'Lambert') during the four ripening stages, to reveal the X-ray diffraction (XRD) diffractograms and Fourier transform infrared (FTIR) spectroscopy data of the kernels, and to evaluate the morphological changes occurring in their structures using scanning electron microscopy (SEM). XRD analysis confirmed that cherry kernels contain both amorphous and crystalline structures. This finding enhances the potential industrial applications of cherry kernels. In the SEM images of all samples, it was observed that the cellulose structure was observed to be layered (graphitic), while the oil exhibited a cellular structure. The oil ratios from these kernels were determined to be between 21.71% ('Lambert') and 28.07% ('Starks Gold') for the 8th week. The highest oil content was obtained in weeks 7 and 8 of the 'Starks Gold' cultivar. Sweet cherry kernel oils are rich in polyunsaturated fatty acids, particularly oleic, linoleic and palmitic acid. The main fatty acids were oleic (35.62-70.60%), linoleic (2.59-69.22%), palmitic (7.40-17.63%) and stearic (2.86-7.52%) acid. The prominent fatty acid of the kernel oils of the sweet cherry was oleic acid. According to the findings of this study, the kernels of sweet cherry cultivars have the potential to produce excellent oil that can be used in edible and other industrial sectors.Öğe A comparative melissopalynological study of royal jelly from Turkey(Taylor & Francis As, 2022) Yil, Gulsah; Karlidag, Semiramis; Akyol, Abuzer; Koseman, Abdurrahman; Yilmaztekin, Murat; Erdogan, Selim; Seker, IbrahimThree treatments (Glucose, Sucrose, Bee Feed Syrup) were used to examine the influence of supplementary feeding on the pollen spectrum of the royal jelly produced by Apis mellifera L. colonies. Apis mellifera colonies were located in the Battalgazi and Dogansehir districts of Malatya province in Turkey. In total 255 royal jelly samples were investigated and the analysis recorded over 30 taxa. In both sites the control group heavily utilised Quercus, Verbascum, Astragalus, and Lamiaceae. The feeding treatments saw Quercus, Verbascum, and Astragalus still being used but in different proportions. The utilisation of Lamiaceae was much reduced and Echium became an important component in colonies that had supplementary feeding.Öğe Cytotoxic and Pro-Apoptotic Effects of Cold-Pressed Hemp Seed Oil on Lung Cells: Cannabinoid and Fatty Acid Profiling via LC-MS/MS and GC-FID(Wiley-V C H Verlag Gmbh, 2025) Yaman, Zeynep; Aydemir, Songul; Ugur, Yilmaz; Demirtas, Sezin; Senturk, Harun; Salva, Emine; Erdogan, SelimThis study aimed to evaluate the cytotoxic and pro-apoptotic effects of cold-pressed hemp seed oil on human lung carcinoma (A549) and normal bronchial epithelial (BEAS-2B) cells. Cell viability was assessed using MTS assay across a concentration range of 1-50 mu g/mL, and a dose-dependent decline was observed in both cell types. At the highest concentration (50 mu g/mL), cell viability decreased to 27% in A549 and 22% in BEAS-2B cells, indicating limited selectivity. Flow cytometry analysis showed that treatment with 25 mu g/mL hemp seed oil resulted in a total late apoptotic/necrotic cell population of 67.13% in A549 cells, with minimal early apoptosis observed. LC-MS/MS analysis confirmed the presence of cannabidiol (CBD, 24.55 mu g/mL) and cannabinol (CBN, 11.91 mu g/mL), with excellent recovery rates (CBD: 96.10 +/- 2.38%, CBN: 98.75 +/- 1.76%) and a validated linear range of 1-1000 mu g/mL (R-2 > 0.99). GC-FID analysis identified a favorable fatty acid profile dominated by linoleic acid (55.7%), alpha-linolenic acid (18.19%), and oleic acid (14.12%), which may contribute synergistically to the observed bioactivity. While these in vitro findings suggest that hemp seed oil may possess anticancer activity, further in vivo studies are warranted to clarify its mechanisms of action, bioavailability, and therapeutic applicability.Öğe Determination of fatty acids in Allium tuncelianum (Tunceli garlic) by gas chromatography with flame ionization detection (GC-FID)(Taylor & Francis Inc, 2021) Ugur, Yilmaz; Karaaslan-Ayhan, Nagihan; Icen, M. Sina; Bicim, Tulin; Erdogan, Selim; Yaman, MehmetGarlic has an important place in daily nutrition. It is consumed by people because of its antimicrobial, antibacterial, and antioxidant properties, and it is also known to be good for many diseases. Along with these properties of garlic, its fatty acid content is also important. The goal of this study is to determine the fatty acids in Allium tuncelianum (Tunceli garlic), which grows naturally in Tunceli, Turkey, by gas chromatography with flame ionization detection (GC-FID). Fifteen of 37 fatty acids studied were detected in Allium tuncelianum, and the primary fatty acids in the sample extracts were determined to be the polyunsaturated fatty acid (PUFA) linoleic acid (C18:2n6c, 41.595%) and the monounsaturated fatty acid (MUFA) oleic acid (C18:1n9c, 33.302%). Small quantities of saturated fatty acids (SAFAs) were also present, including palmitic acid (C16:0, 11.739%), henoicosanoic acid (C21:0, 6.946%), and stearic acid (C18:0, 2.362%).Öğe Determination of Phytochemical Composition in Fruits and Leaves from Different Origins: Black Mulberry, Chokeberry and Elderberry Genotypes(Taylor & Francis Inc, 2024) Zengin, Rukiye; Maras, Zeynep; Ugur, Yilmaz; Oezhan, Onural; Karaat, Firat Ege; Erdogan, SelimBlack mulberry, chokeberry and elderberry have medicinal properties associated with the presence of phytochemicals which are compounds with potential antioxidant properties. The previously published reports have shown that these compounds significantly affect the course of disease processes by counteracting oxidative stress. In this study, the primary anthocyanins were identified using high-performance liquid chromatography - tandem mass spectrometry (LC-MS/MS). Individual phenolic compounds, tocopherols and amygdalin were quantified by high-performance liquid chromatography with diode-array detection (HPLC-DAD). Elemental contents were determined by inductively coupled plasma - optical emission spectroscopy (ICP-OES). The obtained results reveal the significant presence of primary and secondary compounds in the assessed fruits and leaves, with notable variations depending on fruit species and genotypes. The most abundant compounds were phenolic acids and flavonoids. Analyses indicated that chlorogenic acid, rutin, and cyanidin derivatives were predominant phenolic compounds in all fruit species, while phenolic acids and flavonoids were prominent in leaf samples. The highest chlorogenic acid was determined in black mulberry leaves (ranging from 2403.23 to 3542.53 mg/100 g). Generally, the concentration of phenolic compounds were higher in the leaves than in the fruits of the plant. The highest concentration of amygdalin was detected in black mulberry fruits, ranging from 102.12 to 272.46 mg/100 g. Calcium and potassium were the dominant elements in both berries and leaves.Öğe Development of a New Methodology for Determination of Vitamin B9 at Trace Levels by Ultrasonic-Assisted Cloud Point Extraction Prior to HPLC(Springer, 2017) Ulusoy, Halil Ibrahim; Acidereli, Hilal; Ulusoy, Songul; Erdogan, SelimA new analytic methodology based on ultrasonic-assisted cloud point extraction coupled with high-performance liquid chromatography (UA-CPE-HPLC) was developed and successfully applied to determine vitamin B9 in food samples. Polyethylene glycol 6000 (PEG-6000) was used as nonionic surfactant due to its chemical properties and trade availability. All parameters such as surfactant concentration, ionic strength, and the other conditions were also investigated and optimized. The optimal conditions for UA-CPE method were presented with 2.4 % w/v PEG-6000, 14.6 % w/v Na2SO4, and ultrasonic heating assistance at 40 A degrees C for 20 min. The calibration curve for vitamin B9 was linear ranging from 20 to 800 mu g L-1, with correlation coefficients being 0.9944 determined by a HPLC-diode array detector (DAD) detector. Relative standard deviation for 150 mu g L-1 was calculated as 2.87 %. Recovery values in different concentration levels were in the range of 95.6-104.2 %. The obtained results demonstrate that the proposed method can be applied for determination of vitamin B9 in real samples.Öğe DOSE DEPENDENT EFFECTS OF CAFFEIC ACID PHENETHYL ESTER ON HEART RATE AND BLOOD PRESSURE IN RATS(Modestum Ltd, 2005) Iraz, Mustafa; Fadillioglu, Ersin; Tasdemir, Seda; Ates, Burhan; Erdogan, SelimAim: Caffeic acid phenethyl ester (CAPE) is one of the major components of honeybee propolis and its structure is similar to flavonoids. The molecular mechanisms of the effects of CAPE on various systems including cardiovascular system have not been known well. The aim of the present study was to investigate the short term dose dependent in vivo cardiovascular effects including heart rate and blood pressure changes induced by CAPE in Sprague Dawley rats. Methods: The rats were anaesthetized and randomly divided into six groups (n: 6 rats) as follows: the first two groups of rats were injected 0.9% NaCl or 10% alcohol; the other groups were injected 1 mg kg(-1), 5 mg kg(-1) 10 mg kg(-1) or 20 mg kg(-1) CAPE i.v. Results: CAPE injection caused decrease in mean blood pressure (MBP) up to 20 sec. for 1 mg CAPE group and up to 2 min for 5 and 10 mg CAPE groups. On the other hand, heart rate (HR) was found to be decreased up to 10 min. for 10 mg CAPE group Conclusion: CAPE causes decrease in both HR and MBP and may affect conduction velocity and contractility in heart due to possible effects on neuronal transmission.Öğe The effect of adrenomedullin on rats exposed to lead(Wiley, 2008) Dogru, Mehmet Ker; Dogru, Arzu Kocagun; Gul, Mehmet; Esrefoglu, Mukaddes; Yurekli, Muhittin; Erdogan, Selim; Ates, BurhanAdrenomedullin (AdM) was originally discovered as a vasorelaxant peptide. The antioxidative properties of AdM have been reported recently. Through its antioxidative effect, adrenomedullin can protect organs from damage induced by stressors. Lead, commonly detected in air, soil, water and food, is a major source of oxidative stress. The effect of AdM in the liver of rats exposed to lead was investigated. Twenty-four female Wistar rats were divided into four groups: a control group (C), adrenomedullin group (AdM), lead (Pb) group and lead + adrenomedullin (Ph + AdM) group. In the Pb-treated groups, the animals were exposed to lead in drinking water containing 250 ppm PbCl2 for 4 weeks. In the AdM-treated group, the animals received an i.p. injection of AdM (3000 ng kg(-1) body weight) in the third week of lead treatment for I week. The activities of catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) and the level of malondialdehyde (NIDA) were determined in the liver of rats. Histological changes in the liver were examined by light and electron microscopy as well. The NIDA levels were increased significantly in the Pb-treated groups, but in the Ph + AdM group the NIDA levels were decreased significantly when compared with the Ph group. AdM reduced hepatic damage in the Pb + AdM group, but the difference in the total histopathological scores between the Pb and Ph + AdM groups was not significant. When the results are taken together, it can be concluded that AdM may have protective or compensating effects in lead toxicity. Copyright (c) 2007 John Wiley & Sons, Ltd.Öğe The effect of hydrogen peroxide used in desulfurization of dried apricot on the antioxidant capacity and phenolic compound content of the fruit(Springer, 2021) Ugur, Yilmaz; Erdogan, SelimThe effect of hydrogen peroxide (H2O2) on the removal of sulfur dioxide (SO2) from sulfurized dried apricots was studied. Apricot fruits were sulfurized in a way that contains low, medium, and high doses of sulfur. Dried apricots were immersed in 0.5%, 1%, and 2% H2O2 solutions at 20 degrees C, 30 degrees C, and 40 degrees C for 5 min. After removal of SO2 by H2O2, the analyses of moisture, SO2 content, total phenolic content (TPC), individual phenolics, and antioxidant capacity were carried out in dried apricots. As part of the validation study for phenolic compounds, the limit of detection (LOD), the limit of quantification (LOQ), and recovery rate were also determined. H2O2 treatment was effective in desulfurizing, by decreasing up 61% rate the SO2 content in dried apricots. However, H2O2 treatment caused a considerable decrease in the fruit's TPC and antioxidant capacity. Gallic acid, chlorogenic acid, p-coumaric acid, rutin, and myricetin were found to be predominant phenolic compounds in thae dried apricot samples. The amounts of these phenolic compounds (especially in the low dose sulfur-containing samples) with the increase of temperature and H2O2 concentration decreased statistically significant levels (p < 0.05). Therefore, the development and use of methods to preserve the nutritional content of the fruit are important.Öğe Effects of 85 dB Noise Exposure on Macro and Trace Element Levels in Plasma and Brain Areas of Rats(Humana Press Inc, 2013) Karakoc, Yunus; Orum, Mehmet Hamdi; Kilic, Muhammed; Dursun, Mehmet Fatih; Erdogan, SelimIn this study, our hypothesis was that workplace noise can alter brain element levels like immobilization and light-dark cycle shift, and we aimed to investigate the effects of workplace noise on element levels of brain areas as well as changes in blood of chronically noise-exposed rats. Twenty-four rats were randomly divided into three groups. The first group was the control. The second group (noise exposed) was subjected to daily 8 h 85 dB workplace noise for 15 days, and the third group (noise exposed plus normal condition) was also subjected to the same noise exposure and then returned to normal condition for 15 days. In noise-exposed group, Na, K, Ca, Mg , Fe, Cu, and Zn levels in plasma significantly increased when compared with controls (p = 0.03 for Na; p = 0.005 for K and Mg; p = 0.002 for Ca and Fe; p = 0.01 for Cu and Zn). In this group, Fe level of temporal lobe significantly increased (p = 0.021) while Mg level significantly increased in frontal (p = 0.021) and temporal (p = 0.001) lobes when compared with controls. In the noise-exposed plus normal condition group, plasma Na, K, Fe, Cu, and Zn levels were greatly similar to controls. But, plasma Ca and Mg levels significantly decreased when compared with controls (p = 0.002 and p = 0.007) and noise-exposed group (p = 0.002 and p = 0.001). Fe level of temporal lobe significantly increased when compared with controls (p = 0.001) and noise exposed group (p = 0.001). These alterations may be attributed to impaired intake of water and micronutrients or their excretions, emerging by acoustic stress.Öğe Effects of black mulberry, chokeberry, and elderberry extracts on the healing of burn wounds(Elsevier Sci Ltd, 2025) Zengin, Rukiye; Erdogan, Selim; Ozhan, Onural; Karaca, Elif Taslidere; Ozcinar, Semih; Yilmaztekin, Yakup; Yagin, Fatma HilalBackground: In the burn affected area of the skin, the progression or deepening of wounds is related to oxidative stress. Especially in the highly susceptible stasis zone, tissues survive to the extent that they can cope with oxidative stress. Objective: This study investigated the potential of extracts (E) derived from the fruits (F) and leaves (L) of elderberry (E), chokeberry (C), and black mulberry (M), which are rich in antioxidant properties, to enhance the recovery of the stasis zone in burn wounds. Methods: The study employed a sample size of 56 healthy rats. The comb burn model was used. The rats were administered the extracts via gastric gavage for a period of 21 days. Histological examination and biochemical analyses were conducted on biopsies taken from the stasis zone on the 3rd, 7th, and 21st days of the study. Photography was performed for macroscopic evaluations. The histological assays included the evaluation of inflammatory cell infiltration, reepithelialization, and collagenization, as well as immunohistochemical analysis of vascular endothelial growth factor (VEGF). Bioassays pertaining to the enzymatic activities of catalase, superoxide dismutase, glutathione peroxidase, and malondialdehyde (MDA) levels were performed. Results: In macroscopic evaluation, a significant difference was found between the groups in terms of stasis area (F=3.58, p2< 0.001). Post-hoc analyses showed that there was a significant difference between CFE-ELE, EFEBurn, ELE-Burn and MLE-Burn groups in terms of stasis zones (p < 0.05). However, the difference between the groups according to time was not significant (F=1.36, p = 0.16). At the end of the 21-day experiment, inflammatory cell infiltration was higher in the burn group compared to the other groups, but similar to the CFE group. Re-epithelialization was similar in the burn group compared to the fruit extract groups and significantly lower compared to the leaf extract groups. Furthermore, a significant increase in collagenization and VEGF immunoreactivity was observed in all treatment groups compared to the burn group (p < 0.05). However, no significant difference was detected between the treatment groups. The treatment groups presented a notable reduction in MDA levels in comparison to the burn group (p < 0.001). Conclusion: This study demonstrated the efficacy of fruit and leaf extracts in burn healing. Histological examination revealed that leaf extracts exhibited superior healing effects compared to fruit extracts. These results suggest that bioactive components in fruits and leaves may have different biological effects.Öğe Effects of caffeic acid phenethyl ester on pancreatitis in rats(Academic Press Inc Elsevier Science, 2008) Turkyilmaz, Serdar; Alhan, Etem; Ercin, Cengiz; Vanizor, Birgul Kural; Kaklikkaya, Nese; Ates, Burhan; Erdogan, SelimBackground. This study investigated the effect of caffeic acid phenethyl ester (CAPE) on acute necrotizing pancreatitis (ANP) induced by glycodeoxycholic acid in rats. CAPE, an active component of honeybee propolis, has previously been determined to have antioxidant, anti-inflammatory, antiviral, and anticancer activities. Materials and methods. Forty-eight rats were divided into four groups of 12. Group 1 animals received intraductal saline and intravenous saline infusion treatment. Group 2 was given intraductal saline and intraperitoneal CAPE infusion treatment. ANP was induced in the animals in group 3 (ANP with saline infusion), and group 4 had induced ANP plus CAPE infusion treatment (ANP with CAPE infusion). Sampling was performed 48 h after treatment. Results. ANP induction significantly increased mortality rate, pancreatic necrosis, and bacterial infection in pancreatic and extrapancreatic organs. ANP also increased levels of amylase and alanine aminotransferase (ALT) in serum, increased levels of urea and lactate dehydrogenase in bronchoalveolar lavage fluid (BAL LDH), increased the activities of myeloperoxidase (MPO) and malondialdehyde (AIDA) in pancreas and lung tissue, and decreased the serum calcium levels. The use of CAPE did not significantly reduce the mortality rate but significantly reduced the ALT and BAL LDH levels, the activities of MPO and MDA in the pancreas, the activity of MDA in the lungs, and pancreatic damage. The administration of CAPE did not reduce the bacterial infection. Conclusions. These results indicate that CAPE had beneficial effects on the course of ANP in rats and suggest that CAPE shows promise as a treatment for ANP. (c) 2008 Elsevier Inc. All rights reserved.Öğe Evaluation of polyphenol contents in differently processed apricots using accelerated solvent extraction followed by high-performance liquid chromatography-diode array detector(Taylor & Francis Ltd, 2011) Erdogan, Selim; Erdemoglu, SemaConcentrations of 17 polyphenols in ripe-fresh, sun-dried and sulfited-dried apricots either harvested from organic or pesticide-treated trees before harvest were determined using reverse-phase high-performance liquid chromatography with UV-Vis diode array detector, and the change of polyphenol profile with origin of the apricot product was established. Extraction of polyphenols was achieved using the accelerated solvent extraction technique (ASE). A mixture of methanol, water (70:30 v/v) including 0.1 g of tert-butylhydroquinone as solvent, 60 min extraction time, 60 degrees C temperature and 1,500 psi pressure were found the most productive operating conditions for ASE. Concentrations of polyphenols in organic apricots were higher than pesticide-treated samples, and roughly the same in ripe-fresh and sun-dried apricots. Polyphenol concentrations of either organic or pesticide-treated sulfited-dried apricots were lower than the ripe-fresh apricots. Considering the organic ripe-fresh apricots, there was a decrease in some polyphenol concentration in the sulfited-dried apricots compared with the ripe-fresh apricot, and the range of decrease is between 1.4 and 53%.Öğe Evaluation of Relationship between Sperm Cell Velocities and Fatty Acids Contents of Semen Seminal Fluid in the Two Trout Fish Species(Zoological Soc Pakistan, 2023) Ozgur, Mustafa Erkan; Erdogan, Selim; Aydemir, Songul; Yumusakbas, HaticeIn this study, the fatty acid compositions of semen seminal plasma in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) were analyzed in fresh semen by gas chromatography (GC) and investigated the relationships with the sperm cell velocities. According to our results, total levels of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) for Salmo trutta fario occurred in higher quantities than Oncorhynchus mykiss but the quantities of total polyunsaturated fatty acids (PUFA) were less. Palmitic and stearic acid for SFA, oleic and nervonic acids for MUFA and linolenic, arachidonic, eicosapentaenoic and docosahexaenoic acids for PUFA of semen seminal plasma were main. Semen volume and the levels of SFA (myristic, palmitic and margaric acid), MUFA (palmitoleic, ginkgolic, oleic and nervonic acid) and PUFA (linoleic, gama-linolenic and eicosadienoic acid) were significantly different (p<0.05) between both species. Especially, the levels of eicosapentaenoic (EPA) and docosahexaenoic (DHA) determined no significant different (p>0.05). Additionally, we found negative correlation between the straight line velocity (VSL) and pentadecanoic acid (r =-0.78, p<0.05), stearic acid (r =-0.76, p<0.05) and nervonic acid (r =-0.89, p<0.01) for rainbow trout, while there was negative correlation (r =-0.96, p<0.05) between angular path velocity (VAP) and EPA for brown trout. Finally, it can be concluded with these results that the fatty acids of sperm seminal plasma affect the velocities of sperm cells and they would also improve semen quality and fertilization protocols.Öğe Fruit quality and biochemical characteristics of new early ripening apricots of Turkey(Springer, 2021) Cuhaci, Cigdem; Karaat, Firat Ege; Ugur, Yilmaz; Erdogan, Selim; Asma, Bayram MuratIn this study, the important fruit quality characteristics of 11 early ripening and promising apricot hybrids and reference cultivars recently developed in Turkey were analyzed. A large variation was observed between the genotypes in terms of fruit size (fruit weight ranged from 34.28 to 72.53 g). The total soluble solids which is one of the significant fruit quality parameters for the table apricots, was found between 10.07 and 18.27% and fruit flesh/kernel rate was between 9.6 and 18.42. Epicatechin, rutin, catechin, chlorogenic acid, epigallocatechin gallate and beta-carotene were found to be the highest bioactive compounds. A large variation was also determined among the genotypes in this regard. ABP-4-23 and ABP-7-12 apricot genotypes were rich with respect to catechin, chlorogenic acid, epicatechin, rutin and beta-carotene. The highest catachin and epicatechin contents were obtained in ABP-4-23 (176.76 and 517.45 mg/kg, respectively). In terms of beta-carotene ABP-2-24 and ABP-1-18 presented the highest values (127.45 and 119.54 mg/kg, respectively). Fruit weight was found highly and positively correlated with kernel weight and total soluble solids (r = 0.65 andr = 0.61, respectively). Fruit weight was also found to be moderately correlated with rutin, quercetin, and beta-carotene, while corrrelations was negative for rutin and beta-carotene. Total acidity was moderately correlated with catechin, epigallocatechin gallate and beta-carotene in positive way.Öğe Heavy metal pollution and physiological changes in the leaves of some shrub, palm and tree species in urban areas of Adana, Turkey(Taylor & Francis Ltd, 2012) Doganlar, Zeynep Banu; Doganlar, Oguzhan; Erdogan, Selim; Onal, YunusThe effects of anthropogenic pollution on metal accumulation and some physiological parameters were investigated in plant leaves in the Adana, located in the Mediterranean region of Turkey. Washingtonia filifera L., Eucalyptus camaldulensis L., Phoenix dactylifera L., Platanus orientalis L., Citrus aurantium L., Nerium oleander L. and Schinus molle L. plants were collected from four different locations having different pollution levels in Adana. Concentrations of metals (Al, B, Ba, Fe, Pb, Sr and Zn), pigment (chloropyhll and carotenoid), lipid peroxidation product (malondialdehyde), and peroxidase (POD) enzyme activity were determined in the leaf tissues of these plants. Nerium oleander was determined to be a suitable biomonitor for heavy metal accumulation from anthropogenic and traffic activities in Adana. It was also determined that S. molle accumulated more Cu, Pb and Zn than all the other plant species and that metal accumulation did not cause a significant increase in pigment content of this plant. Our results indicate that POD enzyme activities in P. orientalis (Sr and Zn), C. aurantium (Ba) and N. oleander (Cu and Pb) can be used as suitable indicators for heavy metal stress. Additionally, we propose that S. molle can be used as a biomonitor for Pb, Cu and Zn, while N.oleander can be used as a biomonitor for Fe, Al, and Ba.Öğe An investigation of histopathological changes and bioaccumulation in tissues of rainbow trout (Oncorhynchus mykiss) after exposure to dodine(Taylor & Francis Ltd, 2022) Buyuksoylu, Semih; Ozgur, Mustafa Erkan; Gul, Cemile Ceren; Taslidere, Asli; Aydemir, Songul; Erdogan, SelimThe purpose of this research is to determine ecotoxicological effects of dodine (n-dodecylguanidini acetate) on aquatic environments. Though dodine is widely used as a fungicide in agriculture, but there is no much data about its ecotoxicology. In this regard, we investigated bioaccumulation levels and histological alterations on the tissues of muscle, liver and gills in Rainbow Trout (Oncorhynchus mykiss) against different doses (0.01, 0.1, 0.5 and 1mg/L) of Dodine exposure. The tissues of fish were extracted according to QUECHERS method and analyzed by mass spectrometer (LC-MS-MS). Neither of the applied dodine doses resulted in killing 50% of the total individuals in the experimental groups. However, 48hours after doses, behaviors such as instability, anomaly in swimming or sudden jumping movements were observed. Histological results of the study showed deteriorations of the radiological pattern of hepatocytes, sinusoidal dilatations, hemorrhages, edemas, mononuclear cell infiltrations, vascular congestions, hyperplasia and hypertrophy in liver, gill and muscle tissues. Accumulation of dodine in tissues correlated with increase of dose. The maximum level of active substance accumulation in tissues were measured 96hours after application of 1mg/L dodine dose -in order- in gills, muscles and liver. The accumulations were statistically significant (p<0.05) when compared with control group.
