Yazar "Ertas M." seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    Comparison of classifications of seizures: A preliminary study with 28 participants and 48 seizures
    (2005) Baykan B.; Ertas N.K.; Ertas M.; Aktekin B.; Saygi S.; Gokyigit A.; Akyol A.
    Purpose: Our aim was to compare three available seizure classifications (SCs), namely, the international classification of epileptic seizures published in 1981 (ICES; Epilepsia 1981;22:489-50); the semiological seizure classification (SSC) by H. Lüders, J. Acharya, C. Baumgartner, et al. (Epilepsia 1998;39:1006-13; Acta Neurol Scand 1999;99:137-41); and the proposal of a new diagnostic scheme for seizures (PDSS) by J. Engel, Jr. (Epilepsia 2001;42:796-803) published in 2001. The three SCs were compared with respect to diagnostic success rates, usefulness, and consistency by a large group of neurologists in this preliminary study. Methods: After a training period, 28 blindfed participants with different levels of experience with epilepsy classified videos or written descriptions of 48 randomly selected seizures according to the three SCs. Definite diagnoses of the seizures were established based on all clinical, ictal/interictal EEG, and MRI data. All the participants answered a questionnaire concerning their preferences for SCs after the study. Results: The overall diagnostic success rates were 81.4% for ICES, 80.5% for PDSS, and 87.5%, for SSC. Various parameters concerning experience with epilepsy affected success rates positively, without reaching statistical significance, whereas experience with epilepsy surgery appeared to be a parameter significantly affecting the success rate in all SCs. In reliability analysis, Cronbach's ? was 0.94 for ICES, 0.88 for PDSS, and 0.70 for SSC, all showing good agreement in the group. Nineteen reviewers chose SSC, eight chose ICES, and one chose PDSS as their preference in the questionnaire, completed after the end of the study. Conclusion: The results of this preliminary study demonstrate that with proper training, physicians treating epilepsy patients can handle new SCs, and emphasize the need for revision of the current classification. © 2005 Elsevier Inc. All rights reserved.
  • Küçük Resim Yok
    Öğe
    Identification of biodiversity of some Streptomyces species and determination of a restriction fragment length polymorphism (RFLP) profile of 16S rDNA gene region
    (Medwell Journals, 2014) Ozdemir K.; Ogun E.; Ertas M.; Acar S.; Atalan E.
    In this study, soil samples from the roots and peripheral regions of some agricultural plant species growing in the Van region (such as sainfoin, bean, malus, wheat and sugar beet) were collected between May and June 2004 and 139 strains of Streptomyces bacteria were isolated from these samples. Moreover, researchers determined the physicochemical characteristics of these soil samples. Streptomyces bacteria were color-grouped and the Diaminopimelic Acid (DAP) forms were detected followed by the determination of then phenotypic characteristics. When these isolates were divided into 39 color groups, 20 strains were found to contain L-DAP and therefore were confirmed as members of the genus Streptomyces. The species were identified using the TAXON Software and similarity dendrograms were constructed by the Unweighted Pair-Group Method with an Arithmetic average (UPGMA) cluster analysis using the Multi-Variate Statistical Package (MVSP 3.1). Genomic DNA was isolated from these bacteria and 16S rDNA gene was amplified by PCPv followed by restriction digestion with Bspl43I, Haelll, Mnll and SphI for Restriction Fragment Length Polymorphism (RFLP) analysis. In the dendrogram obtained at the end of the RFLP analysis, four groups of two or more members and six 1 -member groups were established according to the similarity ratio criterion. © Medwell Journals, 2014.
  • Küçük Resim Yok
    Öğe
    Use of random amplified polymorphic DNA PCR to distinguish Micromonospora species isolated from soil
    (Medwell Journals, 2014) Ertas M.; Ozdemir K.; Atalan E.
    Recently with the advancement of molecular methods, studies that determine intraspecific genetic polymorphisms have yielded substantial results. The aim of this study was to use Random Amplified Polymorphic DNA PCR (RAPD-PCR) analysis to determine the differences among Micromonospora species isolated from the soil. M3 medium with antibiotics was used to isolate the species from the soil and 65 Micromonospora species were identified. Total DNA analysis was performed for the different color groups which were obtained as a result analysis of the species. Sm5R, Sm6F, DAF4, Ml 3, 27F and 1492R primers were used for RAPD-PCR analysis and the band profiles of the species were obtained. The dendrogram generated according to the simple matching coefficient method clearly showed the differences detected among the species. A total of seven groups were identified along with two main groups. Five of these groups had a single member, one had 3 and one had 2 members. © Medwell Journals, 2014.