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    ANTIOXIDANT RESPONSES OF RAINBOW TROUT (Oncorhynchus mykiss) GILLS AFTER EXPOSURE TO HYDROGEN PEROXIDE
    (Parlar Scientific Publications (P S P), 2015) Seker, Engin; Ispir, Unal; Yonar, Serpil Mise; Yonar, M. Enis; Turk, Cebrahil
    We evaluated antioxidant responses of Oncorhynchus mykiss, experimentally exposed to different concentrations of hidrogen peroxide (H2O2). Different concentrations of H2O2 (50 and 100 ppm) were administrated to the fish for 30, 60 and 90 min. Samples of the gill were collected at the end of the experiment, and analysed for their oxidant antioxidant status, including the malondialdehyde (MDA) level, the catalase (CAT) and glutathione peroxidase (GSH-Px) activity as well as the reduced glutathione (GSH) concentration. Results obtained showed that H2O2 significantly increased MDA level. Compared to controls, GSH level and GSH-Px and CAT activities were significantly reduced in the fish that were exposed to different concentrations of H2O2. The current data suggest that H2O2 has an oxidative-stress-inducing potential in fish. The various parameters studied in this investigation can also be used as biomarkers of exposure to H2O2.
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    Effects of Hexachlorocyclohexane (HCH-?-Isomer, Lindane) on the Reproductive System of Zebrafish (Danio rerio)
    (Central Fisheries Research Inst, 2016) Yuksel, Hayati; Ispir, Unal; Ulucan, Aykut; Turk, Cebrahil; Taysi, M. Resit
    In this study, histopathological changes induced by lindane a gamma isomer of hexachlorocyclohexane and its intoxication associated with reproductive system and hormone levels were investigated in zebrafish. In all, 320 zebrafish adults approximately 1 year in age, (Danio rerio) obtained from a commercial entity were used in the study. Zebrafish were divided into 8 groups, each one containing 40 zebrafish. Groups were organized as 0 (the control group), 1 ml/L methanol, and 5, 10, 20, 40, 80, 160 mu g/L/day lindane. In the study, the gamma-HCH-isomer was applied at rates of 5/10/20/40/80/160 mu g/L/day as doses of immersion to each group for 21 days. Macroscopically, the drop in egg production was observed, especially in the 80 and 160 mu g/L/day groups of female zebrafish. A microscopic decrease in ovulation and biochemical decreases in estradiol (E-2) levels (40/80/160 mu g/L) were also observed in the female zebrafish. As a result, lindane was found to cause changes in the reproductive system, and consequently, to cause hormonal disorders and to have significant effects on ovulation and fertilization in the female zebrafish.
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    Efficacy of lipopolysaccharide antigen of Yersinia ruckeri in rainbow trout by intraperitoneal and bath immersion administration
    (Elsevier Sci Ltd, 2014) Ispir, Unal; Dorucu, Mustafa
    In this study, Intraperitoneal (IP) and bath immersion (BI) vaccine trials were conducted in fish with a mean weight of 6.3 g. Rainbow trout vaccinated with lipopolysaccharide (LPS) was 50 mg/L protein concentration and challenged by IP injection with 9.8 x 10(6) cell/ml of Yersinia ruckeri at 45 days post-immunization had a relative percent survival (RPS). To obtain an effective bath immersion vaccine against yersiniosis, LPS preparation was obtained from the Y ruckeri and with the LPS antigen. After 28 and 60 days vaccinated fish with first and second immunizations by LPS were challenged via intraperitoneal injection with 9.8 x 10(6) cell/ml of Y. ruckeri for evaluating the mortality rates and calculating the relative percentage of survival (RPS). RPS value of experimental groups, which was significantly (P < 0.05) larger than that of the control group. (C) 2014 Elsevier Ltd. All rights reserved.