DSpace Arşivi :: by Yazar "Karabulut, Aysun Bay" değerine göre listeleniyor

Yazar "Karabulut, Aysun Bay" seçeneğine göre listele

Listeleniyor 1 - 20 / 50

Acute and chronic effects of electroconvulsive treatment on oxidative parameters in schizophrenia patients
(Pergamon-Elsevier Science Ltd, 2011) Kartalci, Sukru; Karabulut, Aysun Bay; Ozcan, Abdul Cemal; Porgali, Esra; Unal, Suheyla
Electroconvulsive therapy (ECT) is an effective treatment alternative for schizophrenia. Previous studies have already indicated the possible effects of oxidative stress in this disorder. However, there have been no previous studies evaluating the effects of ECT on the oxidative stress in these patients. We therefore aimed to investigate the acute and chronic effects of ECT on serum levels of oxidant and antioxidant molecules in schizophrenia patients (n = 28). The serum MDA and CAT levels of the patients with schizophrenia were higher than that of the controls before ECT (n = 20) but there was no significant difference in the serum NO and GSH levels of the patient groups compared to the controls. We found that the NO levels of the patients were higher than the controls in the group experiencing their first episode but not in the chronic group. There was a significant clinical improvement in the patients in terms of BPRS. SANS and SAPS reduction after the 9th ECT, but not the 1st ECT. Serum MDA levels were significantly reduced compared to the baseline after the 9th ECT session although there was no significant difference after the 1st session. Separate evaluation of the patient groups revealed that the significant MDA decrease following ECT was in the patients experiencing their first episode and not in the chronic group. No significant difference was noted in the serum levels of other oxidant and antioxidant molecules after either the 1st or 9th ECT session. These results suggest that ECT does not produce any negative effect on oxidative stress in patients with schizophrenia. (C) 2011 Elsevier Inc. All rights reserved.
Apricot attenuates oxidative stress and modulates of Bax, Bcl-2, caspases, NF?-B, AP-1, CREB expression of rats bearing DMBA-induced liver damage and treated with a combination of radiotherapy
(Pergamon-Elsevier Science Ltd, 2014) Karabulut, Aysun Bay; Karadag, Nese; Gurocak, Simay; Kiran, Tugba; Tuzcu, Mehmet; Sahin, Kazim
We evaluated the ability of apricot to attenuate apoptosis and oxidative stress developed during the process of 7,12-dimethylbenz[a]anthracene (DMBA) and radiotherapy in the liver of rats bearing liver damage. Fifty female Wistar rats were divided into 7 groups; (i) normal control rats; (ii) rats fed with standard diet with apricot (20%), (ii) rats fed with standard diet and administrated 6 gray radiotherapy with Co 60 device applied to a single fraction, (iv) rats fed with standard diet and administered intraperitoneally DMBA (20 mg/kg), (v) rats fed with standard diet and administered DMBA and 6 gray radiotherapy, (vi) rats fed with standard rat diet and administered DMBA and supplemented apricot, (vii) rats fed with standard diet supplemented apricot administered DMBA and radiotherapy (RT) for 6 weeks. Expression of Bax, caspase 3, and glutathione activity decreased in the liver but liver expression of NF-kappa B, AP-1, CREB, Bcl-2 and ALT, AST, 5'NT, MDA, NO levels increased in DMBA-induced liver damage rats. In conclusion, the results suggest that apricot supplementation and irradiation given in combination, offer maximum protection against DMBA-induced hepatic carcinogenesis. (C) 2014 Elsevier Ltd. All rights reserved.
Asymmetric dimethylarginine level in hyperglycemic gestation
(Springer, 2011) Sertkaya, Ayse Cikim; Kafkasli, Ayse; Turkcuoglu, Ilgin; Karabulut, Aysun Bay
We aimed to evaluate plasma asymmetric dimethylarginine (ADMA) concentrations and its relation with insulin sensitivity/resistance indices in pregnant women with different degrees of carbohydrate intolerance. This study included a two step approach; 50 g glucose challenge test (GCT) followed by 100 g oral glucose tolerance test (OGTT) was used for diagnosis of carbohydrate intolerance within 24-28th weeks of gestation. Pregnant women with positive GCT but negative OGTT (AGCT group, n = 30) and gestational diabetics (GDM group, n = 58) were compared to healthy pregnant controls (n = 50). Plasma ADMA concentration and its relationship with glucose and insulin levels and insulin sensitivity/resistance indices (HOMA-IR, QUICKI, ISIOGTT) were evaluated. Both AGCT and GDM groups were found to have similarly higher plasma ADMA levels than control subjects (3.60 +/- 1.21; 4.00 +/- 1.70; 2.65 +/- 0.82 mu mol/l, respectively, P = 0.001). ADMA was significantly but slightly correlated with insulin sensitivity/resistance indices and moderately correlated with 2-h insulin level. The 2-h insulin value of the OGTT was the independent influencing constant for ADMA (R = 0.57, P = 0.0001). In conclusion, plasma asymmetric dimethylarginine level was higher in cases with abnormal glucose challenge test but normal OGTT as well as in gestational diabetics, compared to pregnant women with normal glucose tolerance. The elevated ADMA level in pregnant women with carbohydrate intolerance may possibly be due to elevated insulin level.
BIOCHEMICAL EFFECT EVALUATION OF MICROBIAL CHONDROITIN SULPHATE IN EXPERIMENTAL KNEE OSTEOARTHRITIS MODEL
(Parlar Scientific Publications (P S P), 2022) Erenler, Ayse Sebnem; Karabulut, Aysun Bay; Sevimli, Resit; Geckil, Hikmet; Akpolat, Nusret; Unver, Tuba; Otlu, Onder
Osteoarthritis (OA) is the most common chronic joint disease, primarily due to aging. Chondroitin sulfate (CS) is a glycosaminoglycan (GAG) commonly used to treat osteoarthritis. CS can delay the progression of pathology or reverse morphological changes in joint structure. Traditionally CS is produced from animal sources. However, due to different reasons such as contamination, ecological risk, and the possibility of infectious diseases, the trend towards microbial sources has increased because of its advantages such as purer, more antiallergic, and lower Molecular Weight (MW) than animal sources. Biochemical analysis of Microbial CS (MCS), which is a new and significant alternative as a source of CS in the OA healing process, has not been evaluated in the literature yet. This study was designed to analyze the biochemical effects of MCS produced by our team from a microbial source, with an MW value of 269 Daltons, on the osteoarthritis healing process compared to the commercial foiut. We aim to reach data that MCS has a higher antioxidant effect than animal -sourced CS, and in this way, it is a more suitable production for the treatment of osteoarthritis. In this study, knee osteoarthritis was surgically induced in experimental rabbits; and TGF113, CAT, MPO, TOS, and OSI parameters measured in blood samples before the operation and after the healing period were analyzed comparatively. After the surgical application, the rabbits were randomly divided into three groups: control, animal -sourced CS, and E. cull sourced. MCS. The standard rabbit diet was administered daily to 10 rabbits in Group 1 (control), and. CS and MCS were applied daily to the other groups as 17 mg/kg for 12 weeks. Blood samples were taken from rabbits at the 12th week after surgery, and TGF-113, CAT, MPO, TOS, and OSI parameters were biochemically evaluated. This study has confirmed that the antioxidant properties of MCS and data on its effectiveness in controlling oxidative stress compared to animal -sourced CS. Based on these results, it can be concluded that MCS has a significant potency of nutraceutical and therapeutic agents for OA treatment.
Cloning and Expression vgb-kfo Genes in E. coli and Microbial Chondroitin Sulfate Production
(Amer Scientific Publishers, 2019) Erenler, Ayse Sebnem; Geckil, Hikmet; Karabulut, Aysun Bay; Akpolat, Nusret; Sevimli, Resit; Ulke, Esra; Aliyeva, Aygun
This study focused on a new plasmid and new recombinant strain developed for the production of microbial chondroitin sulfate a new and limited area of study the strategies we applied for the production of microbial chondroitin sulfate, and the possible contributions of this study to published research literature. In this study, pETM6-PACF, which carries the genes responsible for capsular chondroitin synthesis [kfA, kfoC, kfoF] was used as the basic plasmid. The Vitreoscilla hemoglobin gene region was transformed into this basic plasmid and the common expression of both gene groups was added to research literature for the first time. This plasmid was transferred to non-pathogenic E. coil (C2987) to produce a completely new chondroitin source specific to this study. Following the transformation by chondroitin synthesis, and the subsequent microbial production of chondroitin by the application of purification protocols, microbial chondroitin sulfate was produced in sulfate form. Consequently, in comparison to published literature, a product with a low molecular weight value of 269 Daltons was developed. This product, which has significant potential drug potency, can be used in many different areas as a novel and unique biomedical product.
Çocukluklarda idrar yolu enfeksiyonu lokalizasyonunda serum amiloid A'nın yeri
(Genel ve Dahili Tıp, 2007) Tabel, Yılmaz; Mungan, İlker; Aktar, Amine; Karabulut, Aysun Bay; Güngör, Serdal
Öz: Amaç: Çocuklarda idrar yolu enfeksiyonu (ÎYE) lokaiizasyonunu saptamada zorluklarla karşılaşılabilmekteclir. Serum Amiloid A (SAA) ise değişik enfeksiyöz ve inflamatuvar durumlarda artan ve başlıca karaciğerden yapılan güçlü bir akut faz reaktanıdır. Biz bu çalışmada, çocuk hastalarda İYE'nun lokalizasyonunda SAA'nm yerini araştırmayı amaçladık. Yöntem: Çalışmaya inönü Üniversitesi Tıp Fakültesi Çocuk Sağlığı ue Hastalıkları kliniğinde ayaktan veya yatırılarak tedavi edilen 22 idrar yolu enfeksiyonlu çocuk alındı. Tedavi başında hastaların koltuk altı uücut ısıları ölçüldü, lökosit, C-reaktif protein (CRP), eritrosit sedimentasyon hızı (ESH), serum amiloid A için kan Örnekleri, rutin idrar analizi ve idrar kültürleri için idrarları alındı. Veriler bilgisayar ortamında SPSS 11.5 paket programında One-Sample Kolmogorov-Smirnov, Student t, Mann-Whitney U, Ki-kare testleri ue Spearman's korelasyon analizi kullanılarak değerlendirildi. P<0.05 anlamlı kabul edildi. Bulgular: Çalışmaya 22 hasta (11 kız, 11 erkek) dahil edildi. Ateş, lökositoz, CRP, ESH yüksekliğine göre hastaların 11'i alt, 11'i ise üst İYE olarak değerlendirildi. İki grup arasında lökosit sayısı, CRP, ESH ue SAA açısından yapılan karşılaştırmalarda sadece CRP ue ESH açısından istatistiksel olarak anlamlı fark tespit edildi (p<0.05). Serum amiloid A ile lokalizasyon arasında anlamlı ilişki saptanmadı (p>0.05). CRP ve SAA arasında korelasyon tespit edilemedi (r=-0.25, p>0.05). Sonuç: Bu çalışmada çocukluk çağı idrar yolu enfeksiyonu lokalizasyonu ile serum amiloid A arasında anlamlı ilişki bulunmadı. Aynca üst idrar yolu enfeksiyonu ile anlamlı ilişkili bulunan C-reaktif protein ile serum amiloid A arasında da anlamlı ilişki tespit edilmemiştir.
Combinatorial effect of zoledronic acid and irradiation on the prevention of DMBA-induced precancerogenic changes in the mammary tissues of rats
(Wolters Kluwer Medknow Publications, 2016) Gurocak, Simay; Karabulut, Aysun Bay; Tuzcu, Mehmet; Sahin, Nurhah; Temelli, Oztun; Ince, Volkan; Sahin, Kazim
Background: At present, the rates of breast cancer are continuously increasing, with over a million new cases being diagnosed worldwide each year. Hence, the development of new breast cancer chemopreventive drugs with acceptable efficacy and toxicity that are suitable for use for a protracted period of time is urgently needed. The present study investigated the potential preventive effects of zoledronic acid [ZOL] and radiotherapy [RT], both alone and in combination, on precancerogenic changes on the breast tissues of females. Materials and Methods: Wistar rats were treated with 7,12-dimethylbenz [a] anthracene [DMBA] at the acute phase. Fifty female rats were divided into seven groups: Control group [I]; ZOL, group [II]; RT, group [III]; DMBA, group [IV]; DMBA + RT, group [V]; DMBA + ZOL, group [VI]; and DMBA + ZOL + RT, group [VII]. Results: The treatment of DMBA-exposed rats with ZOL and RT, both alone and in combination, successfully upregulates the transcriptional levels of Bax, caspase-3, caspase-9, p21, and BRCA 1 in mammary tissues, which may account for the elevated apoptotic activities observed and the eventual inhibition of tumor growth. The administration of RT and ZOL both alone and in combination was found to be effective for inhibiting the DMBA-induced precancerogenic changes on breast tissues and modulating the expression of apoptosis-associated proteins in the acute phase. Conclusions: The combination of RT and ZOL was more effective than either agent alone. Our results suggest that the administration of ZOL and irradiation in combination can offer maximal protection against DMBA-induced mammary precancerogenic changes.
A comparison of erythrocyte superoxide dismutase and catalase activity in patients with hepatitis C infection
(Turkish Journal of Medical Sciences, 2002) Karabulut, Aysun Bay; Sönmez, Emine; Bayındır, Yaşar; Gözükara, Engin
Abstract: Hepatitis C virus (HCV) infection remains important due to difficulties in treatment up to a chronic state. It is considered that free radicals, lipid peroxidation and antioxidant defense play a role in various tissue damages, just as in certain types of viral hepatitis. Since only limited data has been reported concerning oxidative stress in viral hepatitis, a comparative study was planned for patients with hepatitis C. In this study, we searched for erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities in patients with HCV infection who had received and not received recombinant interferon alpha. Four groups of patients [Group I: 15 healthy, volunteers served as a control group; Group II: 10 patients with acute HCV infection; Group III: 15 untreated patients with chronic HCV infection; and Group IV: 15 patients who completed six months of interferon therapy (9 million U/week)] were included in the study. In Group I, SOD activity (as means ± standard deviation) was 2213.29 ± 152.01 U/g Hb; in Group II, 2643.03 ± 142.44 U/g Hb; in Group III, 1135.79 ± 122.27 U/g Hb; and in Group IV, 1734.78 ± 183.72 U/g Hb. The difference between the groups was statistically significant (p < 0.05). Erythrocyte means ± standard deviation CAT levels in Group I were 252.10 ± 61.09 K/g Hb; in Group II, 253.37 ± 29.68 K/g Hb; in Group III, 291.80 ± 72.54 K/g Hb; and in Group IV 220.43 ± 36.39 K/ g Hb. The difference between Groups I, II and III was not statistically significant (p > 0.05), but was for Groups III and IV (p < 0.005) In conclusion, erythrocyte SOD activity increased in acute hepatitis C patients, but decreased in chronic hepatitis C patients, and this decrease was reversed when treated with interferon.
Does the high sulfur content in apricots affect oxidative stress? Running title: Effect of sulfur amount on oxidative stress
(2022) Otlu, Önder; Kıran, Tuğba Raika; Gül, Mehmet; Önal, Yunus; Çolak, Cemil; Karabulut, Aysun Bay
Apricots are one of the most important fruits that can be produced worldwide. Most of the importer countries are sensitive about sulfur which is used in the protection of dried apricots. The objective of this study was to compare the effects of feeding with high and low sulfur content apricots on oxidative stress parameters of the liver. In total, 42 Wistar albino rats were fed for 12 weeks with the use of dried apricots containing different amounts of sulfur and sun-dried apricots. There was no significant difference in IL-1?, 8-OHdG and TOS levels between the control group and the group fed with sun dried apricots. There was no statistically significant difference between the groups fed with low and high sulfur-containing apricots.Non-necrotic changes were observed in 3000 ppm and 4000ppm groups in microscopic examination. No significant effect of feeding with sulfur treated apricots was found in the liver tissues of rats.
Dust-mites: Effect on lipid peroxidation
(All India Inst Medical Sciences, 2006) Atambay, Metin; Karabulut, Aysun Bay; Aycan, Ozlem Makbule; Kilic, Eser; Yazar, Suleyman; Saraymen, Recep; Karaman, Ulku
Background. Dust-mites are present in our homes, feed on dead exfoliated skin and other organic material. It is also known that oxidative stress may lead to cellular damage that can be confirmed by markers of cellular disruption. Oxidative stress in various infective processes has been documented. We investigated whether house dust-mites cause oxidative stress in patients. Methods. Products of lipid peroxidation in erythrocytes and lymphocytes were assessed by measuring malondialdehyde concentration. Results, Our results showed that patients who had a positive skin test for dust-mite antigens and had dust-mites present in their houses (dust-mite positive) had increased erythrocyte malondialdehyde levels (62.39 [18.56]. nmol/g-Hb) compared with those who were skin test positive, dust-mite negative (45.45 [10.82]) or skin test negative, dust-mite negative (42.20 [5.68]). They also had significantly higher levels of lymphocyte malondialdehyde (4.22 [0.55] nmol/g-protein), compared with those who were skin test positive, dust-mite negative (3.46 [0.29]) or skin test negative, dust-mite negative (1.25 [0.31]; p < 0.05). However, there was no statistically significant difference between the malondialdehyde levels of dust-mite negative/skin test positive and dust-mite negative/skin test negative patients. Conclusion. Increased malondialdehyde activity in lymphocytes and erythrocytes in the dust-mite positive/skin test positive group shows the presence of the oxidative stress in patients with dust-mite infestation.
The Effect of Dust-Mites on Nitric Oxide Production
(Tubitak Scientific & Technological Research Council Turkey, 2009) Yazar, Sueleyman; Kilic, Eser; Atambay, Metin; Karabulut, Aysun Bay; Aycan, Oezlem Makbule; Tamer, Guelden Soenmez; Daldal, Nilguen
Aim: We aimed to investigate the effect of house dust mite on the level of nitric oxide in patients who had dust mite in their houses and suffering from rhinitis and/or asthma and/or eczema-like allergic symptoms and were positive for skin tests (Prick). Materials and Methods: The effect of dust-mites on nitric oxide concentration was investigated using Griess method in patients who had allergic symptoms and were positive for skin tests. Results: The plasma nitric oxide level was found to be statistically higher in patients who had dust mite in their houses and suffering from allergic symptoms and were positive for skin tests than in patients who did not have dust mite in their houses but were positive for skin tests or negative for skin tests. In addition. there was no statistical difference in the level of nitric oxide between the patients who did not have dust mite in their houses but were positive for skin tests and the patients who did not have dust mite in their houses and were negative for skin tests. Conclusions: There seems to be an effect of house dust mite on the level of nitric oxide. However, the molecular mechanism underneath of this effect is not yet clear. A plausible explanation for this might be the intrinsic radical properties of nitric oxide and its protective effect against the parasitic agents.
The effect of melatonin against FK506 induced renal oxidative stress in rats
(The effect of melatonin against FK506 induced renal oxidative stress in rats, 2011) Ara, Cengiz; Dirican, Abuzer; Ünal, Bülent; Karabulut, Aysun Bay; Pişkin, Turgut
Background: Nephrotoxicity is an important side effect of FK506 and oxidative stress has been considered as one of the possible mechanisms. The present investigation examines the ability of melatonin to protect against FK506-induced renal oxidative stress. Methods: Thirty rats were divided into 3 groups (n = 10 each group). Group A was the sham group. Group B received 14 days FK506 (5 mg/kg/d, intraperitoneally [i.p.]) and group C received FK506 (5 mg/kg/d, i.p.) together with melatonin (4 mg/kg, i.p.) for 14 days. Kidney tissues were harvested to determine the tissue levels of malondialdehyde (MDA), total nitrite and nitric oxide (NO), tumor necrosis factor-a (TNF-a), and interleukin-6 (IL-6). Results: In group C, the levels of TNF-a, IL-6, and NO were lower than in the group B (P < .01, P < .03, and P < .04, respectively) and although MDA levels were lower than in group B, the differences were not statistically significant (P > .05). Conclusion: These results suggest that melatonin has protective effect against FK506-induced renal oxidative stress.
The Effect of Melatonin Against FK506-Induced Renal Oxidative Stress in Rats
(Sage Publications Inc, 2011) Ara, Cengiz; Dirican, Abuzer; Unal, Bulent; Karabulut, Aysun Bay; Piskin, Turgut
Background: Nephrotoxicity is an important side effect of FK506 and oxidative stress has been considered as one of the possible mechanisms. The present investigation examines the ability of melatonin to protect against FK506-induced renal oxidative stress. Methods: Thirty rats were divided into 3 groups (n = 10 each group). Group A was the sham group. Group B received 14 days FK506 (5 mg/kg/d, intraperitoneally [i.p.]) and group C received FK506 (5 mg/kg/d, i.p.) together with melatonin (4 mg/kg, i.p.) for 14 days. Kidney tissues were harvested to determine the tissue levels of malondialdehyde (MDA), total nitrite and nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6). Results: In group C, the levels of TNF-alpha, IL-6, and NO were lower than in the group B (P < .01, P < .03, and P < .04, respectively) and although MDA levels were lower than in group B, the differences were not statistically significant (P > .05). Conclusion: These results suggest that melatonin has protective effect against FK506-induced renal oxidative stress.
Effects of Electroconvulsive Therapy on Some Inflammatory Factors in Patients With Treatment-Resistant Schizophrenia
(Lippincott Williams & Wilkins, 2016) Kartalci, Sukru; Karabulut, Aysun Bay; Erbay, Lale Gonenir; Acar, Ceren
Objectives Electroconvulsive therapy (ECT) is the most effective option for several psychiatric conditions, including treatment-resistant schizophrenia. However, little is known about the molecular mechanism of action of ECT. The link between inflammatory system and schizophrenia is the focus of recent studies. However, the impact of ECT on inflammatory functioning in this disorder remains elusive. Whether ECT could modulate inflammatory factors in patients with schizophrenia was examined. Methods Plasma levels of interleukin-4 (IL-4), transforming growth factor- (TGF-), myeloperoxidase (MPO), and nuclear factor-B (NF-B) activation were analyzed in 20 schizophrenic patients, mainly with resistant to antipsychotic medication disorders, and in 20 sex- and age-matched healthy controls. Disease severity was evaluated using the Brief Psychiatric Rating Scale. All patients were followed with measurement of the inflammatory factors before and after ECT treatment and compared with the controls. Results Patients with schizophrenia had markedly raised NF-B and but decreased TGF- levels compared with healthy controls. On the other hand, no significant differences were found for the levels of IL-4 and MPO levels. The clinical improvement during repeated ECT was accompanied by a gradual and significant increase in IL-4 and TGF- level, but MPO and NF-B activation were left unaffected. Increases in TGF- were negatively correlated with the change in Brief Psychiatric Rating Scale scores after ECT. Conclusions It is shown that ECT, while increasing the anti-inflammatory response such as the levels of IL-4 and TGF-, it did not affect the levels of MPO and NF-B activation in this study.
Effects of total parenteral nutrition on renal function in preterm neonate
(Turkish Pediatrics Assoc, 2012) Tabel, Yilmaz; Oncul, Mehmet; Akin, Ilke Mungan; Karabulut, Aysun Bay; Elmas, Ahmet Taner
Aim: The aim of this study was to establish serum sistatine C, urine beta(2) microglobulin, gluthatione-S -transferase pi and N-acetyle beta-D glucosaminidase levels in order to evaluate the effect of total parenteral nutrition on renal function in premature infants. In addition, we aimed to compare the renal functions between premature infants receiving total parenteral nutrition and control groups receiving enteral feeding. Material and Method: A hundred four premature infants with a gestational age between 28 and 34 weeks were included in the study. The parenteral nutrition group consisted of 50 infants (male/female; 23/27 and mean weight 1258 +/- 212.3 g) and the enteral nutrition group consisted of 54 infants (male/female; 20/34 and mean weight 1608 +/- 206.1 g). In the parenteral nutrition group; total parenteral group nutrition was initiated on the 3rd day in the enteral nutrition group, minimal enteral nutrition was started on a mean of 6.3 +/- 2.4 days and total enteral nutrition was started on a mean of 24.5 +/- 6.3 days. Breastmilk was given orally or by orogastric/nasogastric tube at first day of life in the enteral group. On the 3rd and 30th day Of life, blood samples of all patients were obtained for evaluating biochemical parameters and cystatin C and urine samples were obtained for evaluation of N-acetyl beta-D glucosaminidase, gluthatione-S-transferase pi, beta(2) microglobulin, sodium, creatinin levels, density and pH of the urine. The study was approved by the ethics commite (2008/16). Results: When we compared the patients who received total parenteral nutrition and enteral nutrition on the 3rd and 30th days, serum cystatin C, urinary beta(2) microglobulin, gluthatione-S-transferase pi and N-acetyl- beta-D glucosaminidase excretions were similar on the 3rd day however were significantly higher on the 30th day in samples of the patients receiving total parenteral nutrition (p<0.05 for each parameter on each day). Conclusions: This study shows that total parenteral nutrition in premature infants can have adverse effects on glomerular and tubular functions of the kidney which can be manifested at an early time with cystatin C, beta(2) microglobulin, gluthatione-S-transferase pi and N-acetyl beta-D glucosaminidase. (Turk Arch Ped 2012; 47: 247-52)
Effects of Verapamil, Nifedipine, and Daflon on the Viability of Reverse-Flow Island Flaps in Rats
(Lippincott Williams & Wilkins, 2013) Kilinc, Hidir; Aslan, Suleyman Serkan; Bilen, Bilge Turk; Eren, Ahmet Tuna; Karadag, Nese; Karabulut, Aysun Bay
Reverse-flow flaps are preferable in reconstructive surgery due to their several advantages. However, they may have venous insufficiency and poor blood flow. In this study, effects of various pharmacological agents on the viability of reverse-flow flaps were investigated. Forty Sprague-Dawley rats were used. Superficial epigastric artery- and superficial epigastric vein-based reverse-flow island flaps were preferred. The rats were divided into 4 groups. Group 1 was considered as the control group. Group 2 was given verapamil 0.3 mg/kg per day, group 3 nifedipine 0.5 mg/kg per day, and group 4 Daflon 80 mg/kg per day for 7 days. On day 7, viable flap areas were measured, angiography was performed, serum nitric oxide levels were evaluated, and histopathological examination was done. The mean flap viability rate was 67.59%(+/- 13.12259) in group 1, 77.38% (+/- 4.12506) in group 2, 74.57% (+/- 3.44780) in group 3, and 85.39% (+/- 4.36125) in group 4 (P = 0.001). The mean nitric oxide level was 31.66 mu mol/dL (+/- 2.42212) in group 1, 51.00 mu mol/dL (+/- 2.96648) in group 2, 34.00 mu mol/dL (+/- 2.96648) in group 3, and 47.66 mu mol/dL (+/- 2.80476) in group 4 (P=0.001). On angiography, there were vessel dilations and convolutions in group 2; capillaries became noticeable, and anastomotic vessels extended toward the more distal part of the flaps in group 4. Histological examinations showed severe inflammation in group 3 and minimal inflammation and venous vasodilatation in group 2. Verapamil and Daflon in therapeutic doses significantly increased the viability of reverse-flow island flaps. However, nifedipine did not make a significant contribution to the flap viability. The results of this study will contribute to the literature about the hemodynamics of reverse-flow island flaps and guide further studies on the issue.
Enterobius vermicularis ile enfekte hastalarda rnalondialdehid, glutatyon ve nitrik oksit düzeyleri
(Mikrobiyoloji Bülteni, 2010) Kıran, Tuğba Raika; Karaman, Ülkü; Çolak, Cemil; Karabulut, Aysun Bay; Daldal, Nilgün
Öz: Başlık (İngilizce): Malondialdehyde, glutathione and nitric oxide levels in patients vvith Enterobius vermicularis infection Öz (İngilizce): The aim of this study was to investigate the levels of malondialdehyde (MDA) and nitric oxide (NO) which are indicators of oxidative stress and also the level of glutathione (GSH) which is an antioxidant molecule, in patients with Enterobius vermicularis infection. A total of 41 patients (mean age: 36.4 years; 31 were male) and 40 healthy controls (mean age: 40.2 years; 28 were male) were included to the study. None of the patients and controls have had history of hormone/steroid drug use, smoking and alcoholism. The mean level of GSH in patient and control groups were found 1.17 ± 0.02 nmol/l and 2.49 ± 0.10 gmol/l; MDA 26.97 ± 2.06 Timol/I and 19.47 ± 2.25 rimol/l; NO 20.74 ± 0.60 umol/dl and 17.83 ± 0.50 umol/dl, respectively. The mean GSH level in patient group was statistically significantly lower (p< 0.05) than controls, while the mean MDA and NO levels were statistically significantly higher (p< 0.05). These results indicated that the consumption of GSH was increased due to the severity of oxidative stress in patients infected with E.vermicularis. Detailed experimental and clinical studies are required to enlighten the relation of GSH in the pathogenesis of E.vermicularis infection. Since oxidative stress is increased during enterobiosis, the use of antioxidant agents (e.g. vitamins C and E) for the supportive treatment deserves evaluation.
Erken doğmuş yenidoğanlarda total parenteral beslenmenin böbrek işlevleri üzerine etkileri
(2012) Tabel, Yılmaz; Öncül, Mehmet; Akın, İlke Mungan; Karabulut, Aysun Bay; Elmas, Ahmet Taner
Amaç: Erken doğmuş yenidoğanlarda total parenteral beslenmenin böbrek işlevleri üzerine etkilerini değerlendirmek için; serumda sistatin C, idrarda ?2 mikroglobülin, glütatyon-S-transferaz ? ve N-asetil ?-D glükozaminidaz düzeylerini saptayarak, total parenteral beslenme alanlar ile enteral beslenen kontrol grubu hastaların böbrek işlevlerinin karşılaştırılması amaçlanmıştır. Gereç ve Yöntem: Bu çalışma; gestasyonel yaşları 28-34 hafta arasında olan 104 erken doğmuş hastada yapıldı. Total parenteral beslenen 50 (Erkek/Kız: 23/27 ve ortalama ağırlıkları 1258±212,3 g) ve enteral beslenen 54 (Erkek/Kız: 20/34 ve ortalama ağırlıkları 1608±206,1 g) erken doğan bebek bu çalışmaya alındı. Total parenteral beslenme alan grupta üçüncü günden sonra total parenteral beslenme başlanırken, minimal enteral beslenmeye ortalama 6,3±2,4 günlerde ve tam enteral beslenmeye ortalama 24,5±6,3 günlerde geçildi. Enteral yolla beslenen gruba ise birinci gün anne sütü orogastrik/nazogastrik sonda ile veya ağızdan başlandı. Tüm hastaların üçüncü ve 30. günlerdeki serumdaki sistatin C, glükoz, BUN, kreatinin, total protein, albümin, AST, ALT, ALP, sodyum, potasyum, kalsiyum, fosfor ve idrardaki; N-asetil-?-D glükozaminidaz, glütatyon S-transferaz ?, ?2-mikroglobülin, sodyum, kreatinin, yoğunluk, pH ölçümleri değerlendirildi. Çalışma için etik kurul onayı alındı (2008/16). Bulgular: Total parenteral beslenme alan hastalar ile enteral beslenen hastaların üçüncü gün değerleri arasında anlamlı fark yokken, 30. gündeki serum sistatin C, idrar ?2 mikroglobülin, glütatyon-S-transferaz ? ve N-asetil ?-D glükozaminidaz değerleri karşılaştırıldığında total parenteral beslenme grubunda enteral beslenenlere göre anlamlı olarak yüksek olduğu saptandı (p<0,05). Çıkarımlar: Bu çalışma; total parenteral beslenmenin böbrekte glomerüler ve tübüler işlevler üzerine olumsuz etkilerinin olabileceğini ve bunun sistatin C, idrar ?2 mikroglobülin, glütatyon-S-transferaz ? ve N-asetil ?-D glükozaminidaz ile erkenden saptanabileceğini göstermesi bakımından anlamlı olmakla beraber, daha geniş serilerde ve daha homojen hasta gruplarında yapılacak çalışmalarla desteklenmesi gerekmektedir.
Evaluation of antioxidant effect of resveratrol on testicular tissue in rats that were exposed to cigarette smoke
(Aves, 2011) Soylemez, Haluk; Ugras, Yahya Murat; Beytur, Ali; Oguz, Fatih; Kurus, Meltem; Karabulut, Aysun Bay
Objective: The aim of this study was to investigate the negative effects of cigarette on testicular biochemistry and histology of rats and to evaluate if resveratrol could alter these effects. Materials and methods: Thirty-two male Wistar Albino rats were divided into 4 grups for an experiment of 6 weeks. Group 1 was used as control; Group 2 was only exposed to cigarette smoke. Group 3 only received intraperitoneal injections of resveratrol (10 mg/kg/day). Group 4 was exposed to both cigarette smoke and resveratrol (10 mg/kg/day). At the end of the experimental period, the rats were sacrified and testes of all rats were removed. Malondialdehyde (MDA), glutathione (GSH), and nitric oxide (NO) levels were measured on testicular tissue and histopathologic examination was performed with light microscope. Results: Weight follow-up of rats revealed no significant difference among groups. MDA level was lower in cigarette smoke and resveratrol group than that of only cigarette smoke group. GSH level showed significant decrease in only cigarette smoke group, while GSH level increased in cigarette smoke and resveratrol group. Differences in NO levels were not statisticaly significant among groups. The histological evaluation revealed that Johnsen score which indicates spermatogenesis was significantly lower only in cigarette smoke rats than all other groups. Conclusion: Oxidative stress develops in testis of rats that were exposed to cigarette smoke, and resveratrol has preventive effects on this damage with direct or indirect antioxidant activity.
Evaluation of asymmetric dimethylarginine, nitric oxide levels and associated independent variables in obese and lean patients with polycystic ovarian syndrome
(Taylor & Francis Ltd, 2011) Turkcuoglu, Ilgin; Engin-Ustun, Yaprak; Turan, Fahri; Kali, Zercan; Karabulut, Aysun Bay; Meydanli, Mutlu; Kafkasli, Ayse
Objective. To evaluate the asymmetric dimethylarginine (ADMA) and nitric oxide (NO) levels in obese and lean patients with polycystic ovarian syndrome (PCOS) and find out their relation with hormonal and metabolic parameters. Methods. Twenty-two obese, 18 lean patients with PCOS and 11 obese, 24 lean healthy control patients were enrolled prospectively. Plasma ADMA and NO levels and arginine/ADMA ratio were evaluated on 3rd day of menstrual cycle after at least 10 h overnight fasting. Results. Plasma ADMA, NO levels and arginine/ADMA ratio were similar in the groups. ADMA level did not correlate with the hormonal and metabolic parameters in patients with PCOS. However, NO correlated inversely with fasting insulin (r = -0.353, p = 0.041) and homeostasis model of insulin resistance (HOMA-IR) (r = -0.379, p = 0.027). Arginine/ADMA ratio also correlated inversely with fasting insulin (r = -0.339, p = 0.050). In multinomial regression analysis the risk of low NO was associated independently with high fasting insulin (OR = 1.19, 95% CI 1.001-1.42, p = 0.049) and high HOMA-IR in patients with PCOS (OR = 2.26, 95% CI 1.03-4.98, p = 0.042). Conclusions. Insulin resistance may be the underlying mechanism of endothelial dysfunction through NO pathway in PCOS.

Yazar "Karabulut, Aysun Bay" seçeneğine göre listele

Sayfa Başına Sonuç

Sıralama seçenekleri