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    ASSESSMENT OF PHYTOTOXIC AND GENOTOXIC EFFECTS OF ANATASE TiO2 NANOPARTICLES ON MAIZE CULTIVAR BY USING RAPD ANALYSIS
    (Parlar Scientific Publications (P S P), 2018) Mutlu, Fatma; Yurekli, Fusun; Mutlu, Birol; Emre, Fatma Bilge; Okusluk, Funda; Ozgul, Onur
    In this study, early phytotoxic and genotoxic effects of chemically synthesized nano-TiO2 (anatase) were investigated in seedlings of maize (Zea mays cv. Hido). Control (Hoagland) and NaCl (300 mM) groups were administered with nano-TiO2 (0.1%, 0.2% and 0.3%). Seed germination and seedling growth parameters of maize cultivar were markedly inhibited by salt stress. Nano-TiO2, without depending on concentration, was found to be ineffective on germination percentage in all administrations. On the contrary, nano-TiO2 caused significant increases in root-stem length and fresh-dry weights especially in NaCl+nano-TiO2 administrations. In salt administered samples, 0.3% nano-TiO2 increased root length 1.4 fold, stem length 4.8 fold, and fresh weight 1.2 fold. Genotoxic properties of nano-TiO2 in seedlings of maize were evaluated by using randomly amplified polymorphic DNA (RAPD-PCR). In RAPD analysis, 20 RAPD primers were found to produce unique polymorphic band profiles at different concentrations of nano-TiO2 maize seedlings. Genomic template stability (GTS), a qualitative measurement of changes in RAPD patterns of genomic DNA, decreased depending on increasing nano-TiO2, NaCl, and NaCl+nano-TiO2 concentrations. Therefore, it could be concluded that nano-TiO2 of appropriate dose can be used to ameliorate negative effects of salt stress by increasing growth potential of maize.
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    Titanium dioxide-multiwalled carbon nanotube/polyimide composite film modified electrodes for simultaneous voltammetric detection of ascorbic acid, uric acid and dopamine as biomarker molecules
    (Springer, 2022) Gungor, Oznur; Ozgul, Onur; Aksoy, Busra; Okusluk, Funda; Koytepe, Suleyman
    Titanium dioxide-multiwalled carbon nanotube/polyimide (TiO2-MWCNT/PI) composite film modified electrodes for simultaneous detection of dopamine (DA), uric acid (UA), and ascorbic acid (AA) in clinical or biomedical applications were prepared. Firstly, TiO2-MWCNT and polyimide structures were synthesized. Then, TiO2-MWCNT/PI composites containing different ratios of MWCNT were subsequently prepared and characterized with chemical, thermal and morphological analysis techniques. Synthesized TiO2-MWCNT/PI composites were coated on the platinum electrode surface. Prepared electrodes were used to sense DA, UA, and AA with voltammetric measurements. LOD and LOQ parameters of modified electrodes for DA were observed 8.5114 mu M and 25.5342 mu M, respectively. The R-2 value for the DA in the linear range from 175 to 325 mu M was obtained 0.9967 (in the existence of AA and UA). After five repeatability results, 96.3% AA, 98.2% DA, and 88.3% UA peak currents were maintained. The modified electrode exhibited good selectivity, acceptable durability, high repeatability, and sensitivity.