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Öğe Alpha lipoic acid decreases neuronal damage on brain tissue of STZ-induced diabetic rats(Pergamon-Elsevier Science Ltd, 2022) Tanbek, Kevser; Ozerol, Elif; Yilmaz, Umit; Yilmaz, Nesibe; Gul, Mehmet; Colak, CemilNeuropathy that develops due to diabetic complications causes cognitive impairment due to functional and structural damage. The aim of this study was to evaluate the biochemical, histological and physiological effects of Alpha Lipoic Acid (ALA) against brain tissue damage caused by diabetes. Fourty male Wistar albino rats were separated into four groups as control, diabetes mellitus (DM), ALA and DM+ALA. Single dose of 50 mg/kg intraperitonal streptozotocin (STZ) was used to induce DM. For six weeks, ALA (100 mg/kg/day) was administered to the ALA and DM+ALA groups. At the end of the six week rats were sacrificed by collecting blood samples and collected brain tissues (hippocampus, cortex, hippotalamus and striatum) were histologically evaluated in addition to the oxidant-antioxidant parameters. ALA administration showed significant improvement in cognitive functions evaluated by MWM in rats with diabetes mellitus (p < 0.05). SOD, CAT, GSH-Px activities, which were decreased in the DM group compared to the control group, increased statistically significantly in rats in DM+ALA group (p < 0.05). While MDA and PC levels increased in the DM group, they decreased statistically significantly in the DM+ALA group (p < 0.05). According to the histological examinations made by light and electron microscopies, it was determined that the ultrastructural damage and degeneration findings observed in the sections of the DM group were significantly ameliorated in the sections of rats in the DM+ALA group. ALA may be effective in restoring cell damage and cognitive functions in brain tissue with its antioxidant and neuroprotective effects without showing antidiabetic effects.Öğe Angiotensin-converting enzyme gene polymorphism and risk of insulin resistance in PCOS(Elsevier Sci Ltd, 2010) Celik, Onder; Yesilada, Elif; Hascalik, Seyma; Celik, Nilufer; Sahin, Ibrahim; Keskin, Lezzan; Ozerol, ElifThe aim of this study was to establish the frequency of angiotensin-converting enzyme (ACE) insertion (I) or deletion (D) gene polymorphism in women with polycystic ovary syndrome (PCOS) and to examine the association of this polymorphism with insulin resistance. A total of 32 women with PCOS and 31 healthy, age- and body mass index-matched controls were studied. Serum lipids, fasting glucose, insulin and other hormones concentrations were measured. Homeostasis model assessment was used to estimate insulin resistance (HOMA-IR). DNA was extracted from peripheral blood leukocytes and genotyping of ACE I/D polymorphism was carried out by polymerase chain reaction. ACE genotypes were distributed as follows: DD was present in 16 (50%), ID in 12 (37.5%) and II in four (12.5%) PCOS patients, and DD in seven (22.6%), ID in 20 (64.5%) and II in four (12.9%) of healthy subjects. The frequency of D and I alleles were found in 69% and 31% of the PCOS group and 55% and 45% in the control group, respectively. There were no significant differences regarding the genotypic distribution and allelic frequency between the groups. However the ACE DD genotype was significantly associated with serum insulin concentrations and HOMA-IR measurement (both P = 0.005). ACE DD genotype is associated with an increased insulin resistance in women with PCOS. (C) 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.Öğe An appraisal of serum preptin levels in PCOS(Elsevier Science Inc, 2011) Celik, Onder; Celik, Nilufer; Hascalik, Seyma; Sahin, Ibrahim; Aydin, Suleyman; Ozerol, ElifPlasma preptin levels were higher in women with PCOS compared with the control group (mean +/- SD, 823.2 +/- 140.7 vs. 324.9 +/- 147.3 pg/mL). This article suggests a potential role of preptin in the pathogenesis of PCOS. (Fertil Steril (R) 2011; 95:314-6. (c) 2011 by American Society for Reproductive Medicine.)Öğe Atorvastatin exerts anti-nociceptive activity and decreases serum levels of high-sensitivity C-reactive protein and tumor necrosis factor-? in a rat endometriosis model(Springer Heidelberg, 2014) Simsek, Yavuz; Gul, Mehmet; Yilmaz, Ercan; Ozerol, Ibrahim Halil; Ozerol, Elif; Parlakpinar, HakanPurpose The purpose of this study was to examine the effects of atorvastatin in the treatment of experimental endometriosis. Methods Endometriosis was induced in 24 female rats. 4 weeks after the procedure dimensions of the foci were recorded. Rats were divided into three groups: in Group 1 (n = 8), a daily dose of 10 mg/kg atorvastatin was given for 14 days. In the second group (n = 8), a single dose of 1 mg/kg leuprolide acetate was injected intraperitoneally. The rats in Group 3 (n = 8) were received 1 mg/kg i.p. 0.9 % NaCl. At the end of the treatment, laparotomy was performed, and the dimensions of the endometriotic foci were recorded. Biochemical, histopathological and immunohistochemical studies were performed and nociception was compared in groups. Results Atorvastatin treatment exhibited significant analgesic activity in hot plate model (P = 0.022). The serum hs-CRP and tumor necrosis TNF-alpha levels were similar between the Group 2 and Group 3 (P > 0.05); however atorvastatin caused significant decrease in both serum markers. The histological and immunohistochemical scores were also found to be markedly lower in Group 1 and Group 2 (P < 0.05). Conclusion Atorvastatin treatment may have a therapeutic potential in the treatment of endometriosis through its anti-inflammatory and anti-nociceptive properties.Öğe Carbontetrachloride induced acute liver damage and protective effect of n-acetylcysteine on rats with regenerated and non-regenerated liver(Walter De Gruyter Gmbh, 2016) Bilgic, Sedat; Ozerol, Elif; Iraz, Mustafa; Sahin, Nurhan; Tanbek, Kevser; Cigli, AhmetObjective: Our aim was to investigate 70% partial hepatectomy (PH) groups, compare with not subjected to PH groups after exposure to hepatotoxic agents for alterations in the protective effects of antioxidant agents and sensitivity of the liver. Accordingly, we aimed to investigate the toxicity of a hepatotoxic agent, carbon tetrachloride (CCl4), and protective effects of an antioxidant, N-acetylcysteine (NAC), in experimental animal model. Methods: 67 male Wistar Albino rats were divided into 2 main groups to total 9 subgroups: group 1, underwent PH; group 2, not subjected to PH. 0.5 ml/kg CCl4 and 50 mg/kg NAC was given intraperitoneally (i.p.) to the groups. On postoperative day 9, 70% PH was performed according to the method of Higgins and Anderson. Finally, all rats were humanely killed. Results: Catalase (CAT) and superoxide dismutase (SOD) activities were significantly lower in both groups when CCl4 was administered. NAC treatment was found to significantly increase these parameters (P<0.05). Malondialdehyde (MDA) and protein carbonyl (PC) levels were significantly greater in both groups when CCl4 was administered (P<0.05). NAC treatment was found to significantly reduce these parameters. Conclusion: These results indicated that CCl4 increased oxidation products, reduced liver enzymatic activity and reduced proliferation activity in both hepatectomised and nonhepatectomised liver. The liver injury of CCl4 and the protective effect of NAC was similarly in both main groups. Consequently, making PH may not create a negative effect and an additional health problems in liver. Thus, these results can positively affect the decisions of the healthy liver donors.Öğe Effects of Alpha Lipoic Acid Learning Behaviors and Histological Examinationon Brain Tissue on Diabetic rats(Wiley, 2017) Tanbek, Kevser; Ozerol, Elif; Gul, Mehmet[Abstract Not Available]Öğe Effects of Recombinant Activated Protein C Derived From Drotrecogin-Alpha on Bleomycin-Induced Pulmonary Fibrosis in Rats Compared with Methyl-Prednisolone(Bilimsel Tip Publishing House, 2013) Yildiz, Kadir; Iraz, Mustafa; Samdanci, Emine; Ozerol, Elif; Kuku, Irfan; Aytemur, Zeynep Ayfer; Hacievliyagil, Suleyman SavasOBJECTIVE: In this study, we aimed to test the preventive effects of intraperitoneally administered drotrecogin alpha which is derived from activated protein C (APC), on bleomycin-induced pulmonary fibrosis in rats, and to compare the effects of APC with the effects of methyl-prednisolone, a traditional therapy. MATERIAL AND METHODS: Thirty male Wistar albino rats were randomly allocated into four groups: 1. Saline alone (n= 6); 2. Bleomycin+ placebo (n= 7); 3. Bleomycin+ methyl-prednisolone (n= 7); 4. Bleomycin+ APC (n= 10). The rats (except for the control group) were given intratracheal bleomycin (2.5 mg/kg). The bleomycin+ APC group was given APC (100 mu g/kg/day) and methyl-prednisolone treated rats were injected with 5mg/kg/day methyl-prednisolone intraperitoneally two days before the bleomycin injection; the drug was administered at the same dose for 16 days. All of the rats were killed 14 days after the intratracheal injection of bleomycin. Fibrotic changes in the lungs were demonstrated by analysing the cellular composition of bronchoalveolar lavage fluid, histological evaluation and lung hydroxyproline content. RESULTS: Fibrosis was experimentally induced in the lungs of rats using bleomycin. Fibrosis scores in the bleomycin+ methyl-prednisolone and the bleomycin+ APC groups were significantly lower than in the bleomycin+ placebo group (p< 0.05). The scores of the bleomycin+ APC group and the bleomycin+ methyl-prednisolone group were similar. The lung tissue hydroxyproline contents in the bleomycin+ placebo and bleomycin+ methyl-prednisolone groups were significantly higher than the control group (p< 0.05), but the hydroxyproline content in the bleomycin+ APC group was significantly lower than in the other groups (p< 0.05). CONCLUSION: Drotrecogin alpha that is derived from recombinant APC has a protective effect on the pulmonary fibrosis induced by bleomycin. The protective effect seen with methylprednisolone is similar.Öğe Elevated cardiac oxidative stress in newborn rats from mothers treated with atosiban(Springer Heidelberg, 2012) Simsek, Yavuz; Celik, Onder; Karaer, Abdullah; Yilmaz, Ercan; Gul, Mehmet; Ozerol, Elif; Bilgic, SedatThe purpose of this study was to evaluate the cardiac and cerebral oxidative stress in the offspings of pregnant rats treated with oxytocin antagonist atosiban. Experimentally naive, adult female Wistar-albino rats (200-250 g) were mated with adult male rats for copulation. After confirming pregnancy, eight gravid rats were then randomly assigned into two equal groups. The animals were treated from days 15 to 20 of gestation. One group acted as a control group, and received intraperitoneal (i.p.) injections of saline in a daily dose volume of 6 mg/kg/day. The second group received 6 mg/kg/day i.p. atosiban. On day 21 of gestation, pups were delivered by cesarean. The heart and brain tissues of the newborn rats were dissected and sent for the measurement of total oxidant status, total antioxitant status and oxidative stress index. There was no significant difference in birthweight or in the number of pups between two groups. Newborns from atosiban-treated mothers showed significantly increased oxidative stress in the plasma and heart tissue than that of controls which was confirmed by histological examination (P < 0.05). Oxidative stress parameters and histopathological results of the brain tissues of newborns were similar between two groups (P > 0.05). Oxytocin receptor blockage for the treatment of premature delivery may be associated with increased fetal morbidity and mortality secondary to the elevated oxidative stress in the heart of the newborns.Öğe Larazotide acetate reduces the frequency of bacterial translocation in the thioacetamide-induced liver failure in rats(Elsevier, 2020) Caliskan, Ali Riza; Harputluoglu, Muhsin Murat; Otlu, Baris; Gul, Mehmet; Ozerol, Elif; Uremis, Nuray; Dertli, Ramazan[Abstract Not Available]Öğe Nuclear transcription factor-kappa beta-dependent ultrastructural alterations within the placenta and systemic inflammatory activation in pregnant patients with hemolysis, elevated liver functions and low thrombocyte count (HELLP) syndrome: a case-control study(Taylor & Francis Inc, 2013) Simsek, Yavuz; Gul, Mehmet; Celik, Onder; Aydin, Nasuhi Engin; Duz, Senem Arda; Celik, Ebru; Ozerol, ElifObjective: Preeclampsia appears to be associated with a higher extent of inflammation than in uncomplicated pregnancies. We aimed to test whether this was the case in patients with hemolysis, elevated liver functions and low platelet count (HELLP) syndrome and to clarify the contribution of placental and systemic inflammatory variables in the development of this syndrome. Materials and methods: Thirty healthy pregnant women (control group) and 20 patients with HELLP syndrome (study group) were included in the study. Placental inflammatory activity was evaluated by quantifying immunohistochemically the levels of p65/RelA expression of nuclear transcription factor-kappa beta (NF-kB) in paraffin-embedded tissue samples. In addition, ultrastructural changes in placental morphology in HELLP patients were evaluated by transmission electron microscopy (TEM). The serum concentrations of myeloperoxidase (MPO) and C-reactive protein (CRP) were also measured and compared. Results: p65/RelA immunoexpression and serum MPO and CRP levels were significantly higher in patients with HELLP syndrome (p < 0.05). TEM of placenta in the study group revealed severely vacuolized syncytiotrophoblasts, irregular basal lamina and damaged capillary endothelium when compared with the placenta of control subjects. Conclusion: Our results suggest that over-expression of placental NF-kB is correlated with elevation of serum inflammatory markers and placental ultrastructural changes, which may point to an important role of local and systemic inflammatory activation in the pathogenesis of HELLP syndrome.Öğe Prediction of spontaneous preterm delivery in women with preterm labor: Analysis of interleukin-17, migration inhibitory factor, monocyte chemotactic protein-1 in maternal serum and cervicovaginal fluid(Walter De Gruyter Gmbh, 2012) Karaer, Abdullah; Celik, Onder; Dogan, Cagdas; Ozerol, Ibrahim Halil; Simsek, Omer Yavuz; Celik, Ebru; Ozerol, ElifBackground: To analyze whether, interleukin (IL)-17, migration inhibitory factor (MIF), monocyte chemotactic protein (MCP)-1 in maternal serum and cervicovaginal fluid, can identify women in preterm labor with intact membranes who will deliver spontaneously within 7 days of sampling. Material and Methods: Forty-four (44) singleton pregnancies between 26-34 weeks of gestation that had threatened preterm labor, enrolled from October 2010 and October 2011. Cervical length was evaluated with transvaginal ultrasonography. Serum and cervicovaginal fluid IL-17, MIF, and MCP-1 were measured with the use of enzyme linked immune assay. Subjects were followed up for premature delivery within 7 days. Results: Seventeen of 44 patients (39%) had preterm delivery and 15 of 44 (34%) delivered within 7 day from admission. The only significant predictor for establishment of preterm delivery within 7 days was cervical length. The median cervical length was 18 mm (range: 5-27) in women who delivered in 7 days and 27 mm (range: 9-44) in women who delivered beyond 7 days of presentation (p-0.001). There was no association between serum and cervicovaginal fluid IL-17, MIF and MCP-1 levels and preterm delivery within 7 days. Conclusions: Cervical length could serve as a predictive factor for the progress of threatened preterm labor to true preterm labor. Serum and cervicovaginal fluid IL-17, MIF and MCP-1 can not be used to differentiate true preterm labor.Öğe Protective Effect of Alpha-Lipoic Acid on Methotrexate-induced Cardiac Injury(Wiley-Blackwell, 2016) Yildiz, Azibe; Tanbek, Kevser; Ozerol, Elif; Vardi, Nigar[Abstract Not Available]Öğe The protective effect of erdosteine on short-term global brain ischemia/reperfusion injury in rats(Pergamon-Elsevier Science Ltd, 2009) Ozerol, Elif; Bilgic, Sedat; Iraz, Mustafa; Cigli, Ahmet; Ilhan, Atilla; Akyol, OmerExperimental studies have demonstrated that free radicals play a major role on neuronal injury during ischemia/reperfusion (I/R) in rats. Erdosteine is a thioderivative endowed with mucokinetic, mucolytic and free-radical-scavenging properties. The aim of the present study was to investigate the effect of erdosteine treatment against short-term global brain ischemia/reperfusion injury in rats. The study was carried out on Wistar rats divided into four groups. (i) Control group, (ii) ischemia/reperfusion group, (iii) ischemia/reperfusion + erdosteine group, and (iv) erdosteine group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities as well as thiobarbituric acid reactive substances (TBARSs) and nitric oxide (NO) levels were analysed in erythrocyte and plasma of rats. Plasma NO levels were significantly higher in the ischemia/reperfusion group than the other groups. The activities of SOD and GSH-Px were decreased, while TBARS levels increased in the ischemia/reperfusion group compared to other groups in both plasma and erythrocyte. The erythrocyte CAT activity was higher in erdosteine group and there was a statistically significant increase, when compared with the erdosteine plus ischemia/reperfusion group. By treating the rats with erdosteine, the depletion of endogenous antioxidant enzymes (SOD, CAT, GSH-Px) and increase of TBARS and NO levels were prevented. This study, therefore, suggests that erdosteine reduces parameters of oxidative stress is well supported by the data. (c) 2008 Elsevier Inc. All rights reserved.Öğe Role of TSH, fT3 and Anti-Thyroid Antibodies on Neurodegeneration of Streptozotocin-Induced Diabetic Rats(Karger, 2018) Tanbek, Kevser; Ozerol, Elif; Taskapan, M. Cagatay; Gul, Mehmet; Ozerol, Beyza[Abstract Not Available]Öğe Role of tsh, ft3 and anti-thyroid antibodies on neurodegeneration of streptozotocin-ınduceddiabetic rats(Karger, allschwılerstrasse 10, ch-4009 basel, swıtzerland, 2018) Tanbek, Kevser; Ozerol, Elif; Taskapan, M. Cagatay; Gul, Mehmet; Ozerol, Beyza
