Yazar "Polat, Alaadin" seçeneğine göre listele
Listeleniyor 1 - 20 / 51
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe The acute effect of humic acid on iron accumulation in rats(Biological Trace Element Research, 2016) Çağın, Yasir Furkan; Şahin, Nurhan; Polat, Alaadin; Erdoğan, Mehmet Ali; Atayan, Yahya; Eyol, Ergül; Bilgiç, Yılmaz; Seçkin, Yüksel; Çolak, CemilAbstract Free iron leads to the formation of pro-oxidant reactive oxygen species (ROS). Humic acids (HAs) enhance permeability of cellular wall and act as a chelator through electron transferring. This study was designed to test chelator effect of HA on iron as well as its anti-oxidant effect against the iron-induced hepatotoxicity and cardiotoxicity. The rats used were randomly divided into four groups (n = 8/group): group I (the control group); group II (the HA group), humic acid (562 mg/kg) was given over 10 days by oral gavage; group III (the iron group), iron III hydroxide polymaltose (250 mg/kg) was given over 10 days by intraperitoneal route; and group IV (the HA plus iron group), received the iron (similar to group II) plus humic acid (similar to those in groups II and III) group. Blood and two tissue samples both from liver and heart were obtained for biochemical and histopathological evaluations. Iron deposition, the iron-induced hepatotoxicity, and cardiotoxicity were demonstrated by histopathological and biochemical manner. However, no significant differences were observed in the serum biochemical values and thehistopathological results among the iron and the HA plus iron groups in the liver tissue but not in the heart tissue. The protective effects of humic acid against iron-induced cardiotoxicity were shown but not against hepatotoxicity in our study.Öğe The acute effect of humic acid on ıron accumulation in rats(Biological Trace Element Research, 2016) Çağın, Yasir Furkan; Şahin, Nurhan; Polat, Alaadin; Erdoğan, Mehmet Ali; Atayan, Yahya; Eyol, Ergül; Bilgiç, Yılmaz; Seçkin, Yüksel; Çolak, CemilFree iron leads to the formation of pro-oxidant reactive oxygen species (ROS). Humic acids (HAs) enhance permeability of cellular wall and act as a chelator through electron transferring. This study was designed to test chelator effect of HA on iron as well as its anti-oxidant effect against the iron-induced hepatotoxicity and cardiotoxicity. The rats used were randomly divided into four groups (n = 8/group): group I (the control group); group II (the HA group), humic acid (562 mg/kg) was given over 10 days by oral gavage; group III (the iron group), iron III hydroxide polymaltose (250 mg/kg) was given over 10 days by intraperitoneal route; and group IV (the HA plus iron group), received the iron (similar to group II) plus humic acid (similar to those in groups II and III) group. Blood and two tissue samples both from liver and heart were obtained for biochemical and histopathological evaluations. Iron deposition, the iron-induced hepatotoxicity, and cardiotoxicity were demonstrated by histopathological and biochemical manner. However, no significant differences were observed in the serum biochemical values and the histopathological results among the iron and the HA plus iron groups in the liver tissue but not in the heart tissue. The protective effects of humic acid against iron-induced cardiotoxicity were shown but not against hepatotoxicity in our study.Öğe Anti-Inflammatory Effects of Resveratrol on the Radiotherapy-Induced Neuroinflammation(2022) Yıldız, Azibe; Ciftci, Tuba; Polat, Seyhan; Gunata, Mehmet; Parlakpınar, Hakan; Temelli, Öztun; Polat, AlaadinObjective: Damage in the central nervous system caused by cranial radiotherapy (RT) has been linked to neuroinflammation due to microglial activation. Evidence reveals that resveratrol (RES) exerts neuroprotective effects by inhibiting neuroinflammation. There are limited studies investigating the effects of RES on microglia-related neuroinflammation developed as a result of RT. Therefore, this study was designed to investigate the effects of RES on RT-induced microglial- related neuroinflammation. Materials and Methods: Rats were simple randomly divided into three groups. Sham (SH) group received ethanol solution on the 1st-14th days of the study. RT group was applied a single dose of total cranial 15 Gy X radiation on the 7th day of the study. RES group was administered a dose of 20 mg/kg on the 1st-14th days of the study and a single dose of total cranial 15 Gy X radiation on the 7th day of the study. The brain tissues removed at the end of the experiment were subjected to histological techniques and procedures for histological and immunohistochemical examinations. The data were evaluated statistically. Results: RT administration caused histological changes such as neuron degeneration and edema in the brain tissue. In addition, RT administration induced a significant increase in CD68 and tumor necrosis factor-alpha (TNF-?) immunoreactivity. In the RES+RT group, it was observed that histological changes were alleviated, and CD68 and TNF-? immunoreactivities were decreased. In addition, a significant increase in the immunoreactivity of autophagy-related proteins was detected in this group. Conclusion: Evaluating together all the data, it was revealed that RES attenuates microglia- mediated neuroinflammation and neuronal degeneration.Öğe Beneficial effects of dexpanthenol on mesenteric ischemia and reperfusion injury in experimental rat model(Taylor & Francis Ltd, 2016) Cagin, Yasir Furkan; Atayan, Yahya; Sahin, Nurhan; Parlakpinar, Hakan; Polat, Alaadin; Vardi, Nigar; Tagluk, Mehmet EminBackground and aim It has been reported that intestinal ischemia-reperfusion (I/R) injury results from oxidative stress caused by increased reactive oxygen species. Dexpanthenol (Dxp) is an alcohol analogue with epitelization, anti-inflammatory, antioxidant, and increasing peristalsis activities. In the present study, the aim was to investigate protective and therapeutic effects of Dxp against intestinal I/R injury. Materials and methods Overall, 40 rats were assigned into five groups including one control, one alone Dxp, and three I/R groups (40-min ischemia; followed by 2-h reperfusion). In two I/R groups, Dxp (500mg/kg, i.m.) was given before or during ischemia. The histopathological findings including apoptotic changes, and also tissue and serum biochemical parameters levels, were determined. Oxidative stress and ileum damage were assessed by biochemical and histological examination. In the control (n=8) and alone Dxp (n=8; 500mg/kg, i.m. of Dxp was given at least 30min before recording), groups were incised via laparotomy, and electrical activity was recorded from their intestines. In this experiment, the effect of Dxp on the motility of the intestine was examined by analyzing electrical activity. Results In ileum, oxidant levels were found to be higher, while antioxidant levels were found to be lower in I/R groups when compared with controls. Dxp approximated high levels of oxidants than those in the control group, while it increased antioxidant values compared with I/R groups. Histopathological changes caused by intestinal I/R injury and histological improvements were observed in both groups given Dxp. In the Dxp group, electrical signal activity markedly increased compared with the control group. Conclusions Here, it was seen that Dxp had protective and therapeutic effects on intestinal I/R injury and gastrointestinal system peristaltism.Öğe Beneficial effects of dexpanthenol on mesenteric ischemia and reperfusion injury in experimental rat model(Free Radical Research, 2016) Yasir, Furkan Çağın; Atayan, Yahya; Şahin, Nurhan; Parlakpınar, Hakan; Polat, Alaadin; Vardı, Nigar; Tağluk, Mehmet Emin; Tanbek, Kevser; Yıldız, AzibeBackground and aim It has been reported that intestinal ischemia–reperfusion (I/R) injury results from oxidative stress caused by increased reactive oxygen species. Dexpanthenol (Dxp) is an alcohol analogue with epitelization, anti-inflammatory, antioxidant, and increasing peristalsis activities. In the present study, the aim was to investigate protective and therapeutic effects of Dxp against intestinal I/R injury. Materials and methods Overall, 40 rats were assigned into five groups including one control, one alone Dxp, and three I/R groups (40-min ischemia; followed by 2-h reperfusion). In two I/R groups, Dxp (500 mg/kg, i.m.) was given before or during ischemia. The histopathological findings including apoptotic changes, and also tissue and serum biochemical parameters levels, were determined. Oxidative stress and ileum damage were assessed by biochemical and histological examination. In the control (n ¼ 8) and alone Dxp (n ¼ 8; 500 mg/kg, i.m. of Dxp was given at least 30 min before recording), groups were incised via laparotomy, and electrical activity was recorded from their intestines. In this experiment, the effect of Dxp on the motility of the intestine was examined by analyzing electrical activity. Results In ileum, oxidant levels were found to be higher, while antioxidant levels were found to be lower in I/R groups when compared with controls. Dxp approximated high levels of oxidants than those in the control group, while it increased antioxidant values compared with I/R groups. Histopathological changes caused by intestinal I/R injury and histological improvements were observed in both groups given Dxp. In the Dxp group, electrical signal activity markedly increased compared with the control group. Conclusions Here, it was seen that Dxp had protective and therapeutic effects on intestinal I/R injury and gastrointestinal system peristaltism.Öğe Beneficial role of aminoguanidine on acute cardiomyopathy related to doxorubicin-treatment(Springer, 2006) Cigremis, Yilmaz; Parlakpinar, Hakan; Polat, Alaadin; Colak, Cemil; Ozturk, Feral; Sahna, Engin; Ermis, NecipDoxorubicin (DOX) is a broad-spectrum anthracycline antibiotic that has cardiotoxicity as a major side effect. One mechanism of this toxicity is believed to involve the reactive oxygen radical species (ROS); these agents likely account for the pathophysiology of DOX-induced cardiomyopathy. Aminoguanidine (AG) is an effective antioxidant and free radical scavenger which has long been known to protect against ROS formation. We investigated the effects of AG on DOX-induced changes in thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) content. The rats were divided into four groups:1) Control; 2) DOX group; injected intraperitoneally (i.p.) with DOX 20 mg/kg in a single dose 3) AG-treated group; injected i.p. in single dose of 20 mg/kg DOX plus 100 mg/kg AG 1 h before the DOX for 3 days, 4) AG group; injected i.p. with AG 100 mg/kg for 3 days. DOX administration to control rats increased TBARS and decreased GSH levels. AG administration before DOX injection caused significant decrease in TBARS and increase in GSH levels in the heart tissue when compared with DOX only. Morphological changes, including severe myocardial fibrosis and inflammatory cell infiltration were clearly observed in the DOX-treated heart. AG reversed the DOX-induced heart damage. Therefore AG could protect the heart tissue against free radical injury. The application of AG during cancer chemotherapy may attenuate tissue damage and improve the therapeutic index of DOX.Öğe Comparison of Antioxidant Effects of Isoflurane and Propofol in Patient Undergoing Right Donor Hepatectomy(Wiley-Blackwell, 2012) Ucar, Muharrem; Ozgul, Ulku; Polat, Alaadin; Toprak, Huseyin I.; Erdogan, Mehmet A.; Aydogan, Mustafa S.; Durmus, Mahmut[Abstract Not Available]Öğe Correlations between N200, P300 and nitric oxide in high intensive exercise among sportsmen trained at various levels(Elsevier Science Bv, 2006) Duzova, Hahl; Ozisik, Handan Isin; Polat, Alaadin; Emre, M. Hanifi; Gullu, Esin[Abstract Not Available]Öğe Dexpanthenol therapy reduces lung damage in a hyperoxic lung injury in neonatal rats(Taylor & Francis Ltd, 2016) Ozdemir, Ramazan; Demirtas, Gulsum; Parlakpinar, Hakan; Polat, Alaadin; Tanbag, Kevser; Taslidere, Elif; Karadag, AhmetObjective: Dexpanthenol (Dxp) plays a major role in cellular defense and in repair systems against oxidative stress and inflammatory response and it has not yet been evaluated in treatment of bronchopulmonary dysplasia (BPD). We tested the hypothesis that proposes whether Dxp decreases the severity of lung injury in an animal model of BPD.Methods: Forty rat pups were divided into four groups: control, control+Dxp, hyperoxia and hyperoxia+Dxp. All animals were processed for lung histology and tissue analysis. The degree of lung inflammation, oxidative and antioxidant capacity was assessed from lung homogenates.Results: Lung injury score and alveol diameter increased in the hyperoxia group (p<0.001). Median level of malondialdehyde, total oxidant status and oxidative stress indexes was significantly higher in the hyperoxia group compared to the other groups. The median superoxide dismutase activity in the hyperoxia group was notably less than those of control+Dxp and hyperoxia+Dxp groups (p<0.01). Similarly, lung catalase, glutathione (GSH) peroxidase and reduced GSH activities in the hyperoxia group were significantly lower than other groups. Furthermore, the hyperoxia+Dxp group had lower tumor necrosis factor- and interleukin-1 median levels compared to the hyperoxia group (p=0.007).Conclusion: Dxp treatment results in less emphysematous change as well as decrease in inflammation and oxidative stress markers in an animal model of BPD.Öğe Dose-Dependent Protective Effect of Ivabradine against Ischemia-Reperfusion-Induced Renal Injury in Rats(Karger, 2012) Beytur, Ali; Binbay, Murat; Sarihan, M. Ediz; Parlakpinar, Hakan; Polat, Alaadin; Gunaydin, M. Orhun; Acet, AhmetBackground/Aims: This study was designed to investigate the dose-dependent protective effect of ivabradine, a specific inhibitor of the cardiac sinoatrial node, on renal ischemia-reperfusion (I/R) injury in rats. Methods: Rats were divided into six groups: group 1, control; group 2, I/R (60 min ischemia followed by 24 h reperfusion); groups 3 and 4, 0.6-6 mg/kg ivabradine; and groups 5 and 6, sham+0.6-6 mg/kg ivabradine. At the end of the study, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase contents were assayed in the kidney tissues; serum blood levels of urea nitrogen (BUN), creatinine (Cr) and albumin also were determined. Results: Tissue MDA levels were found to be significantly higher in the I/R group, whereas SOD and CAT levels were lower when compared to the control group. Ivabradine (0.6 mg/kg) treatment reduced the MDA levels and elevated the SOD and CAT enzyme activity. Treatment with a dose of 6 mg/kg ivabradine further increased MDA levels and did not ameliorate SOD or CAT activities. Serum levels of BUN and Cr were significantly higher in the I/R group. I/R+0.6 mg ivabradine reduced the elevated BUN and Cr levels. Conclusion:This study indicates that ivabradine exerts a dose-dependent response beyond heart rate reduction against renal I/R injury. Copyright (C) 2011 S. Karger AG, BaselÖğe Efeito da genisteina na ototoxicidade induzida pela cisplatina e estresse oxidativo(Assoc Brasileira Otorrinolaringologia & Cirurgia Cervicofacial, 2022) Tan, Mehmet; Toplu, Yuksel; Varan, Emrah; Sapmaz, Emrah; Ozhan, Onural; Parlakpinar, Hakan; Polat, AlaadinObjetivo: A cisplatina e um agente antineoplasico usado em adultos e criancas para o tra-tamento de diversas lesoes malignas. Pode causar ototoxicidade irreversivel. A genisteina e um fitoestrogeno que funciona como antioxidante e inibidor do ciclo celular ao inibir as enzi-mas DNA topoisomerase e tirosina-quinase. O efeito protetor da genisteina na prevencao da ototoxicidade induzida pela cisplatina e os niveis de estresse oxidativo foram investigados. Metodo: Trinta e dois ratos Sprague Dawley foram usados em 4 grupos (controle, cisplatina, cis-platina + genisteina, genisteina). As medidas das emissoes otoacusticas por produto de distorcao foram tomadas nos dias 1, 2 e 5 do protocolo do teste. Foram medidos os niveis sericos de malondialdeido, superoxido dismutase, catalase, glutationa peroxidase, estado antioxidante total, estado oxidante total e indice de estresse oxidativo. Resultados: A audicao do grupo cisplatina + genisteina foi melhor do que a do grupo cisplatina. Enquanto os parametros malondialdeido, estado oxidante total e indice de estresse oxidativo diminuiram significantemente no grupo cisplatina + genisteina em comparacao com o grupo cisplatina, o superoxido dismutase mostrou aumento significantemente (p < 0,05). Conclusao: A genisteina apresentou efeitos positivos contra a ototoxicidade com seu efeito antioxidante. Nivel de evidencia: Nivel 3. (c) 2021 Associacao Brasileira de Otorrinolaringologia e Cirurgia Cervico-Facial. Publicado por Elsevier Editora Ltda. Este e um artigo Open Access sob uma licenca CC BY (http:// creativecommons.org/licenses/by/4.0/).Öğe The effect of caffeic acid phenethyl ester CAPE against cholestatic liver injury in rats(J Surg Res, 0–0., 2010) Çoban, Sacid; Yıldız, Fahrettin; Alpaslan, Terzi; Al, Behcet; Özgör, Dinçer; Ara, Cengiz; Ara, Cengiz; Polat, Alaadin; Esrefoğlu, MukaddesObjectives. Caffeic acid phenethyl ester (CAPE) has been subjected to considerable investigations that have revealed its antioxidant and anti-inflammatory activities in different conditions. But there is not a previous investigation about its effect on cholestatic liver injury. The aim of this study was to investigate the effect of CAPE in rat liver against cholestatic liver injury induced by bile duct ligation. Methods. Swiss-albino rats were recruited in the study as follows; Group 1 rats subjected to simple laparotomy known as the sham group; Group 2 rats subjected to bile duct ligation (BDL); Group 3 bile duct ligated rats treated with CAPE. The third group received CAPE (10 mmol/kg) intraperitoneally daily throughout 14 d. Results. Data showed a decrease in g glutamyl transferase (GGT), aspartate aminotransferase (AST), and alanine aminotransferase levels (ALT) of the CAPE treated rats, compared with BDL group (P < 0.001, P < 0.01, and P < 0.02, respectively). In the CAPE treated rats, tissue levels of malondialdehyde (MDA) and myeloperoxidase (MPO) were significantly lower than that of the BDL group (P < 0.001). The levels of glutathione (GSH) in CAPE treated rats were significantly higher than that of BDL group (P < 0.001). In CAPE treated group, the levels of interleukin1alpha (IL-1a) and interleukin-6 (IL-6) were signifi- cantly lower than that of BDL group (P < 0.03, P < 0.02, respectively). Administration of CAPE in the rats with biliary obstruction resulted in inhibition of necro-inflammation. Conclusion. These results suggest that treatment of CAPE maintains antioxidant defenses, reduces oxidative liver injury, cytokine damage, and necroinflammation in bile duct ligated rats. Thus, CAPE seems to be a promising agent for the attenuation of cholestatic liver injury. 2010 Elsevier Inc. All rights reserved.Öğe The effect of caffeic acid phenethyl ester CAPE against cholestatic liver injury in rats(J Surg Res, 2010) Çoban, Sacid Abdussemet; Yıldız, Fahrettin; Terzi, Alparslan; Al, Behçet; Özgör, Dinçer; Ara, Cengiz; Polat, Alaadin; Eşrefoğlu, MukaddesObjectives. Caffeic acid phenethyl ester (CAPE) has been subjected to considerable investigations that have revealed its antioxidant and anti-inflammatory activities in different conditions. But there is not a previous investigation about its effect on cholestatic liver injury. The aim of this study was to investigate the effect of CAPE in rat liver against cholestatic liver injury induced by bile duct ligation. Methods. Swiss-albino rats were recruited in the study as follows; Group 1 rats subjected to simple laparotomy known as the sham group; Group 2 rats subjected to bile duct ligation (BDL); Group 3 bile duct ligated rats treated with CAPE. The third group received CAPE (10 mmol/kg) intraperitoneally daily throughout 14 d. Results. Data showed a decrease in g glutamyl transferase (GGT), aspartate aminotransferase (AST), and alanine aminotransferase levels (ALT) of the CAPE treated rats, compared with BDL group (P < 0.001, P < 0.01, and P < 0.02, respectively). In the CAPE treated rats, tissue levels of malondialdehyde (MDA) and myeloperoxidase (MPO) were significantly lower than that of the BDL group (P < 0.001). The levels of glutathione (GSH) in CAPE treated rats were significantly higher than that of BDL group (P < 0.001). In CAPE treated group, the levels of interleukin- 1alpha (IL-1a) and interleukin-6 (IL-6) were signifi- cantly lower than that of BDL group (P < 0.03, P < 0.02, respectively). Administration of CAPE in the rats with biliary obstruction resulted in inhibition of necro-inflammation.Conclusion. These results suggest that treatment of CAPE maintains antioxidant defenses, reduces oxidative liver injury, cytokine damage, and necroinflammation in bile duct ligated rats. Thus, CAPE seems to be a promising agent for the attenuation of cholestatic liver injury.Öğe The effect of caffeic acid phenethyl ester on bacterial translocation and intestinal damage in cholestatic rats(Dig Dis Sci (2006) 51:1754–1760, 2006) Ara, Cengiz; Eşrefoğlu, Mukaddes; Polat, Alaadin; Işık, Burak; Aladağ, Murat; Gül, Mehmet; Ay, Selma; Tekerekoğlu, M.Sait; Yılmaz, SezaiWe investigated the effect of caffeic acid phenethyl ester in rat ileum injury induced by chronic biliary obstruction. Swiss albino rats were divided into three groups: Group 1, sham (n = 7); Group 2, common bile duct ligation (n = 7); and Group 3, common bile duct ligation plus caffeic acid phenethyl ester (n = 7). In the caffeic acid phenethyl ester-treated rats, ileum tissue levels of malondialdehyde and myeloperoxidase were significantly lower than those of the bile duct-ligated rats (P < 0.001). The levels of tumor necrosis factor-α, interleukin-6, and interleukin-1α in the caffeic acid phenethyl ester group were significantly lower than those in the bile duct ligation group (P < 0.03, P < 0.01, and P < 0.02 respectively). The present study demonstrates that intraperitoneal administration of caffeic acid phenethyl ester in bile duct-ligated rats reduces intestinal oxidative stress. This effect may be useful in the preservation of intestinal damage in cholestasis.Öğe The effect of dexmedetomidine against oxidative and tubular damage induced by renal ischemia reperfusion in rats(Taylor & Francis Ltd, 2015) Cakir, Murat; Polat, Alaadin; Tekin, Suat; Vardi, Nigar; Taslidere, Elif; Duran, Zeynep Rumeysa; Tanbek, KevserDexmedetomidine (dex) is a potent, highly selective and specific alpha 2-adrenoreceptor agonist. This experimental study was designed to investigate protective and therapeutic effect of two different doses of dex, on kidney damage induced by ischemia-reperfusion (I/R) in rats. Male Sprague - Dawley rats were divided into four groups, each including 10 animals: control group, ischemia-reperfusion (I/R) group; treated groups with 10 mu g/kg of dex and 100 mu g/kg of dex. After removing right kidney of the rats, the left kidney has performed ischemia during 40 min and reperfusion in the following 3 h. The histopathological findings, and also tissue superoxide dismutase (SOD) and catalase (CAT) enzyme activity, malondialdehyde (MDA), glutathione (GSH), serum blood urea nitrogen (BUN), creatinine (Cre) and tumor necrosis factor-alpha (TNF-alpha) levels were determined. In the I/R group, compared to the control group, levels of BUN, Cre and kidney tissue MDA have increased significantly, SOD, CAT enzyme activity and glutathione levels have decreased significantly. In the dex10 group, compared to the I/R group, levels of Cre and TNF-alpha have decreased significantly, while the SOD activity has increased significantly. In the dex100 group, compared to the I/R group, levels of BUN, Cre have decreased significantly, while the SOD activity has increased significantly. In the I/R group, there was also extensive tubular necrosis, glomerular damage in the histological evaluation. Dex ameliorated these histological damages in different amounts in two treatment groups. In this study, the protective effects of dex against renal I/R injury have been evaluated by two different amount of doses.Öğe The effect of melatonin on acetylsalicylic acid induced kidney and testis damage(Human & Experimental Toxicology, 2014) Altıntaş, Ramazan; Polat, Alaadin; Parlakpınar, Hakan; Vardı, Nigar; Beytur, Ali; Oğuz, Fatih; Sağır, Mustafa; Yıldız, Azibe; Duran, Zeynep RümeysaThe aim of this study was to evaluate the acute effect of high-dose acetylsalicylic acid (ASA) on kidney and testis, and the potential protective and therapeutic effects of melatonin on ASA-related pathology. A total of 40 rats were randomly divided into the following 5 groups (n ¼ 8): group 1: control, not given any drug; group 2: only 200 mg/kg ASA was given; group 3: 5 mg/kg melatonin was given 45 min before administering 200 mg/kg ASA; group 4: 5 mg/kg melatonin was given 45 min after administering 200 mg/kg ASA; and group 5: only 5 mg/kg melatonin was given. The histopathological changes and the biochemical findings; such as malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), reduced glutathione (GSH), and blood urea nitrogen (BUN) as well as serum creatinine (Cr) levels were evaluated. ASA significantly increased MDA levels in both kidney and testis, whereas it significantly decreased the values of SOD, CAT, GPX, and GSH in kidney and CAT levels in testis. Melatonin significantly decreased MDA levels in kidney and ameliorated it in testis, whereas it caused elevation in the levels of antioxidants. BUN and Cr levels were higher after ASA, whereas these levels were diminished after melatonin administration. The improvement obtained by melatonin on ASA-induced histological alterations was more prominent when it was used after ASA in kidney and before ASA in testis. In this study, we demonstrated the beneficial effect of melatonin on high-dose ASA-related pathology of kidney and testis for the first time.Öğe Effect of renal colic analgesia on oxidative stress parameters(Academic Journals, 2010) Turtay, Muhammet Gokhan; Oguzturk, Hakan; Colak, Cemil; Polat, Alaadin; Tasdemir, CemalOur aim in this study is to investigate serum oxidative stress parameters before and after fentanyl analgesia in renal colic which is a severe pain condition and to reveal if there is correlation between fentanyl analgesia and serum oxidative stress parameters in renal colic pain. Thirty two male patients, aged 18 - 65, (mean age 40.7 +/- 14.9) who applied to our hospital with the complaint of flank pain and were diagnosed with renal colic were accepted to study. Blood samples were taken from the patients who were diagnosed with renal colic as a result of physical examination and tests (urine analysis, plain radiography, ultrasonography, computed tomography) Pain management of the patients was carried out. Fentanyl (50 - 150 mu g), an opioid analgesic, was used in all patients. When patients stated that the pain was definitely gone through, the blood samples were taken again. Blood samples were taken from each participant twice, before and after the analgesic. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) levels were measured from the blood taken. Statistical analysis of the levels which were obtained from before and after the fentanyl treatment was carried out. There were increases in CAT (P < 0.001), SOD (P = 0.002), MDA (P < 0.001), and GPx (P = 0.28) activities in response after analgesic administration in the patients. In our study, the treatment of renal colic pain with fentanyl, an opioid analgesic, led to significant increases in the levels of SOD and CAT, which are antioxidant enzymes.Öğe Effects of ?- Glucan Liver Ischemia/Reperfusion Injury in Rats(Wiley-Blackwell, 2012) Aydogan, Mustafa Said; Yucel, Aytac; Erdogan, Mehmet Ali; Polat, Alaadin; Cetin, Asli; Ucar, Muharrem; Duran, Zeynep Rumeysa[Abstract Not Available]Öğe The effects of dexmedetomidine on liver ischemia-reperfusion injury in rats(Academic Press Inc Elsevier Science, 2013) Sahin, Taylan; Begec, Zekine; Toprak, Huseyin I.; Polat, Alaadin; Vardi, Nigar; Yucel, Aytac; Durmus, MahmutBackground: Ischemia-reperfusion (IR) injury of the liver may cause various types of damage to hepatic tissues. It can affect the prognosis of patients and the success of an operation. Dexmedetomidine is a selective alpha(2) receptor agonist. We investigated whether dexmedetomidine provides protection against IR-induced liver injury in rats. Methods: Forty rats were divided equally into four groups. In group 1, the liver was manipulated after the laparotomy, and no occlusion of the vessels of the liver was performed. In group 2, once the abdomen was opened, 60 min of ischemia and 60 min of reperfusion were applied according to the segmental hepatic ischemia model. In group 3, 10 mu g/kg of dexmedetomidine was injected into the peritoneal cavity 30 min before ischemia. In group 4, 100 mu g/kg of dexmedetomidine was injected into the peritoneal cavity 30 min before ischemia. Further procedures in groups 3 and 4 were the same as those of group 2. After the experiment was completed, the rats were killed. Liver tissues were removed and stored until biochemical and histologic assessments were performed. Results: The malondialdehyde level in group 2 was higher than that of groups 1, 3, and 4 (P = 0.001, P = 0.000, and P = 0.000, respectively). Superoxide dismutase, catalase, and glutathione levels in group 2 were lower than those in group 1 (P = 0.001, P = 0.027, and P = 0.014, respectively). Superoxide dismutase and catalase levels in group 4 were higher than those in group 2 (P = 0.002 and P = 0.000, respectively). GSH levels in groups 3 and 4 were higher than those in group 2 (P = 0.049 and P = 0.006, respectively). A lower glutathione peroxidase level was detected in groups 2 and 3 than that in group 1 (P = 000). Group 4 demonstrated an increase in glutathione peroxidase levels compared with group 3 (P = 0.014). The histologic injury scores in groups 2-4 were higher than those in group 1 (P = 0.003, P = 0.002, and P = 0.001, respectively). However, the histologic injury scores were lower in groups 3 and 4 than those in group 2 (P = 0.003 and P = 0.002, respectively). Conclusions: This study showed that dexmedetomidine may protect the liver against IR injury in rats. (C) 2013 Elsevier Inc. All rights reserved.Öğe The Effects of Dexmedetomidine on Liver Ischemia-Reperfusion Injury in Rats(Wiley-Blackwell, 2012) Sahin, Taylan; Begec, Zekine; Toprak, Huseyin I.; Polat, Alaadin; Vardi, Nigar; Yucel, Aytac; Durmus, Mahmut[Abstract Not Available]
- «
- 1 (current)
- 2
- 3
- »
