Yazar "Satilmiş B." seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    Evaluation of protective effects of verapamil against lens epithelial tissue injury induced by irradiation in rats
    (Turkish Pharmacists Association, 2010) Batçio?lu K.; Erkal H.S.; SerIn M.; Uyumlu A.B.; Yücel N.; Yildirim B.; Satilmiş B.
    In this study, we investigated the possible protectant effects of verapamil against lens epithelial tissue injury induced by irradiation in rats. Forty female Wistar Albino rats were divided into four groups (n=10). Group 1 received 10 mg/kg verapamil and underwent 20 Gy irradiation. Group 2 received no verapamil and underwent 20 Gy irradiation. Group 3 received 10 mg/kg verapamil and underwent sham irradiation. Group 4 received no verapamil and underwent sham irradiation. The lens tissues were dissected and measured malondialdehyde levels in all groups. The mean malondialdehyde levels (nmol/mg prt) were determined as 5,59 for Group 1, 7,04 for Group 2, 3,95 for Group 3 and 3,25 for Group 4. In conclusion, we can suggest that verapamil as a protectant agent against radiation induced lens epithelial tissue injury.
  • Küçük Resim Yok
    Öğe
    Phenethyl isothiocyanate Regulates the Cancer Stem Cell Phenotype of SNU449 Hepatocellular Carcinoma Cells via STAT3-CD44 Axis
    (Society of Pharmaceutical Sciences of Ankara (FABAD), 2023) Satilmiş B.
    Cancer stem cells play an essential role in resistance to therapy, invasion, metastasis, and recurrence. CD44 is one of the well-known surface markers for hepatocellular carcinoma, and its expression level is related to poor survival and a high recurrence rate. The effect of phenethyl isothiocyanate (PEITC) on SNU449 hepatocellular carcinoma cell line cancer stem cells is not known. The goal of the present study was to investigate whether PEITC regulates the cancer stem cell phenotype of SNU449 cells. Cell viability, colony formation, and wound healing assays were performed to determine proliferative and migratory characteristics. Caspase 3, CD44, Akt/ mTOR, and p38/STAT3 protein expression levels were measured by Western blotting. Compared to control confluence, gap fill, and migration rate were increased while half gap time was decreased in PEITC-treated cells. Compared to control-treated cells, CD44 (3.2-fold) and p-STAT3 (2.44-fold) protein expressions were upregulated in PEITC-treated cells. Results of this study suggest that STAT3-mediated upregulation of CD44 leads to the gain of cancer stem cell phenotype of PEITC-treated SNU449 cells. © 2023 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.