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Öğe The effects of caffeic acid phenethyl ester on cisplatin nephrotoxicity: Increased antioxidant enzyme activity in kidney(2000) Ozbek E.; Cekmen M.; Turkoz Y.Objective: This article was undertaken to investigate the effect of caffeic acid phenethyl ester (CAPE) on nephropathy and antioxidant enzyme activities in kidneys treated with anticancer agent cisplatin. Design: An experimental study. Material and methods: Twenty four adult male Sprague-Dawley rats were used in our study. The rats were divided into three groups of 8 animals each: Controls (injected with saline, group 1), injected with cisplatin, and injected with cisplatin plus CAPE. Cisplatin was injected intraperitoneally (ip) 5 mg/kg at a single dose (group 2). Group 3 was injected with 5 mg/kg cisplatin single intraperitoneal injection plus CAPE 10 :M/kg/d for 3 days. 24 h after the last injection, rats were sacrificed. Then, kidneys were quickly removed and decapsulated. The renal cortex was carefully separated from the medulla and homogenized. Blood samples were collected to determine the serum levels of urea and creatinine. Crude extracts were used to determine Glutathione Peroxidase (GSH-Px), Catalase (CAT), and Superoxide Dismutase (SOD) activities. Results were compared with Mann-Whitney-U test. Results: Cisplatin administration resulted in a significant decrease of GSH-Px, CAT, and SOD activities in the kidney compared to controls. In the rats treated with cisplatin plus CAPE, there was a remarkable restoration in GSH-Px, CAT, SOD activities (p < 0.05). Conclusions: Our results suggest that cisplatin suppresses SOD, CAT, and GSH-Px activities in the kidney, thereby making the tissue more vulnerable to oxidative stress and peroxidative attacks. These findings also indicate that CAPE given concomitantly with cisplatin could protect the kidney tissue against free radical injury because of antioxidative power of CAPE.Öğe Ghrelin and lipid levels in panic disorder before and after treatment and their relationship with agoraphobia(Medicinska Naklada Zagreb, 2015) Gul I.G.; Cumurcu B.E.; Karlidag R.; Turkoz Y.Background: We aimed to evaluate serum ghrelin (GHR) levels and lipid profile in panic disorder (PD), with and without agoraphobia, and to compare these parameters before and after treatment. Subjects and methods: The GHR and lipid profiles were measured in blood samples taken from 31 PD patients with agoraphobia, 22 PD patients without agoraphobia, and 53 control group subjects. 23 of the 53 patients who were prescribed 20 to 40 mg/day paroxetine had continued treatment. The 23 patients who had continued treatment were measured again at the end of twelve weeks. Results: The GHR and triglyceride (TRG), total cholesterol (Total-C), low-density lipoproteins (LDL-C), and very low-density lipoproteins (VLDL-C) levels were higher in the PD with agoraphobia group than the PD without agoraphobia and control groups. The 23 patients that had continued their treatment were re-evaluated, and the serum GHR, Total-C levels, and BMI after treatment were significantly decreased, compared to the values before treatment. Conclusions: There may be a pathophysiological relationship between the GHR and lipid profiles that interact with each other in PD. In fact, this relationship was more marked in PD with agoraphobia than in PD without agoraphobia. © Medicinska naklada - Zagreb, Croatia.Öğe The protective effect of N-acetylcysteine against acrylamide toxicity in liver and small and large intestine tissues(Comenius University, 2015) Altinoz E.; Turkoz Y.; Vardi N.The aim of this study was to investigate the protective effects of N-acetylcysteine against acrylamide toxicity in liver and small and large intestine tissues in rats. The rats were divided into four groups. Acrylamide administration increased MDA levels in all tissues significantly (p < 0.05). But acrylamide+NAC administration decreased MDA levels significantly as compared to the acrylamide group, and lowered it to a level close to the control group values (p < 0.05). GSH levels in liver and small intestine tissues reduced significantly in the acrylamide group (p < 0.05). But acrylamide+NAC administration increased GSH levels significantly in all tissues. Whereas GST activity decreased significantly in the acrylamide group in liver and small intestine tissues as compared to the other groups (p < 0.05), the GST activity increased significantly in the acrylamide+NAC group in all tissues as compared to the acrylamide group (p < 0.05). Liver histopathology showed that the liver epithelial cells were damaged significantly in the acrylamide group. Small intestine histopathology showed that the intestinal villous epithelial cells were damaged significantly in the acrylamide group. Our results indicate that a high level of acrylamide causes oxidative damage in liver and small and large intestine tissues, while N-acetylcysteine administration in a pharmacological dose shows to have an antioxidant effect in preventing this damage (Tab. 2, Fig. 2, Ref. 66). Text in PDF www.elis.sk.Öğe Protective effect of saffron (its active constituent, crocin) on oxidative stress and hepatic injury in streptozotocin induced diabetic rats(Gene Therapy and Molecular Biology, 2014) Altinoz E.; Oner Z.; Elbe H.; Turkoz Y.; Cigremis Y.The objective: the reactive oxygen species (ROS) take role in pathogenesis of many diseases like diabetes. Saffron extract, crocin and safranal are remarkable ROS scavenging as antioxidant agents. Methods and results: rats were divided into three groups each containing 10 as follows: control group, Diabetes Mellitus (DM) group, and Diabetes Mellitus+crocin (DM+crocin) group. Tissue samples were processed by routine histological and biochemical procedurs. Liver tissue of control group showed normal histological appearance. Sinusoidal dilatation, sinusoidal congestion, infiltration and vacuolization were observed in hepatocytes of DM group. These findings were reduced in DM+crocin group. The MDA and XO levels in DM group were higher than the other groups (P<0.01), and GSH levels in DM+crocin group were higher than DM group (P<0.01). Blood glucose concentration in DM group increased (p=0.002) compared to control group, but decreased in DM+crocin group (p=0.002) compared to DM group. Serum alanine aminotransferase (ALT) and aspartat aminotransferase (AST) levels increased remarkably (P?0.01) in DM group compared to control group. When DM+crocin group was compared with DM group, serum ALT levels decreased (p?0.05); however, decrease was lower in serum AST level (p>0.05). Conclusion: we observed in our study that crocin decreased blood glucose level of STZ induced diabetic rats and protected the liver tissue by decreasing the oxidative stress.Öğe The protective role of N-acetylcysteine against acrylamide-induced genotoxicity and oxidative stress in rats(Gene Therapy and Molecular Biology, 2014) Altinoz E.; Turkoz Y.Acrylamide is neurotoxic, genotoxic and highly carcinogenic in humans and animals. The aim of this study was to research the protective role of N-acetylcysteine against acrylamide-induced genotoxicity and oxidative stress in rats. In the present study, male wistar albino rats were divided into four groups, each containing 10 as follows: Control (C) group, Acrylamide (AA) group, N-acetylcysteine (NAC) group, Acrylamide + N-acetylcysteine (AA+NAC) group. At the end of 21 days, Malondialdehyde (MDA) and Glutathione (GSH) levels were measured in plasma and DNA damage was observed in lymphocytes. Plasma GSH level decreased significantly in AA group compared to C group (P < 0.05). However, GSH level increased significantly in AA+NAC group compared to AA group (p< 0.05). MDA levels increased significantly in AA group compared to C group (p< 0.05). But AA+NAC administration decreased MDA levels as compared to AA group (p< 0.05). Comet analysis results showed that lymphocyte DNAs were normal appearance in C and NAC groups. DNA damage was observed at a highest level in AA group. Furthermore some lymphocytes exhibited apoptotic appearance. However this damage was prevented by NAC administration on lymphocyte DNAs significantly. Our results demonstrated that high level of AA caused oxidative stress and DNA damage, but NAC could have a protective role on acrylamide-induced toxicity.Öğe Quercetin protection against ciprofloxacin induced liver damage in rats(Taylor and Francis Ltd, 2016) Taslidere E.; Dogan Z.; Elbe H.; Vardi N.; Cetin A.; Turkoz Y.Ciprofloxacin is a common, broad spectrum antibacterial agent; however, evidence is accumulating that ciprofloxacin may cause liver damage. Quercetin is a free radical scavenger and antioxidant. We investigated histological changes in hepatic tissue of rats caused by ciprofloxacin and the effects of quercetin on these changes using histochemical and biochemical methods. We divided 28 adult female Wistar albino rats into four equal groups: control, quercetin treated, ciprofloxacin treated, and ciprofloxacin + quercetin treated. At the end of the experiment, liver samples were processed for light microscopic examination and biochemical measurements. Sections were prepared and stained with hematoxylin and eosin, and a histopathologic damage score was calculated. The sections from the control group appeared normal. Hemorrhage, inflammatory cell infiltration and intracellular vacuolization were observed in the ciprofloxacin group. The histopathological findings were reduced in the group treated with quercetin. Significant differences were found between the control and ciprofloxacin groups, and between the ciprofloxacin and ciprofloxacin + quercetin groups. Quercetin administration reduced liver injury caused by ciprofloxacin in rats. We suggest that quercetin may be useful for preventing ciprofloxacin induced liver damage. © 2015 The Biological Stain Commission.
