The effects of caffeic acid phenethyl ester on cisplatin nephrotoxicity: Increased antioxidant enzyme activity in kidney
Küçük Resim Yok
Tarih
2000
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: This article was undertaken to investigate the effect of caffeic acid phenethyl ester (CAPE) on nephropathy and antioxidant enzyme activities in kidneys treated with anticancer agent cisplatin. Design: An experimental study. Material and methods: Twenty four adult male Sprague-Dawley rats were used in our study. The rats were divided into three groups of 8 animals each: Controls (injected with saline, group 1), injected with cisplatin, and injected with cisplatin plus CAPE. Cisplatin was injected intraperitoneally (ip) 5 mg/kg at a single dose (group 2). Group 3 was injected with 5 mg/kg cisplatin single intraperitoneal injection plus CAPE 10 :M/kg/d for 3 days. 24 h after the last injection, rats were sacrificed. Then, kidneys were quickly removed and decapsulated. The renal cortex was carefully separated from the medulla and homogenized. Blood samples were collected to determine the serum levels of urea and creatinine. Crude extracts were used to determine Glutathione Peroxidase (GSH-Px), Catalase (CAT), and Superoxide Dismutase (SOD) activities. Results were compared with Mann-Whitney-U test. Results: Cisplatin administration resulted in a significant decrease of GSH-Px, CAT, and SOD activities in the kidney compared to controls. In the rats treated with cisplatin plus CAPE, there was a remarkable restoration in GSH-Px, CAT, SOD activities (p < 0.05). Conclusions: Our results suggest that cisplatin suppresses SOD, CAT, and GSH-Px activities in the kidney, thereby making the tissue more vulnerable to oxidative stress and peroxidative attacks. These findings also indicate that CAPE given concomitantly with cisplatin could protect the kidney tissue against free radical injury because of antioxidative power of CAPE.
Açıklama
Anahtar Kelimeler
Antioxidant enzyme activity, Caffeic acid phenethyl ester, Cisplatin, Nephrotoxicity
Kaynak
International Medical Journal
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
7
Sayı
2
