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Öğe Cytotoxic effects of orthodontic composites.(2010) Malkoc S.; Corekci B.; Ulker H.E.; Yalçin M.; Sengün A.OBJECTIVES: To evaluate the cytotoxic effects of five different light-cured orthodontic bonding composites. Materials and Methods: The orthodontic composites Heliosit Orthodontic (Ivoclar), Transbond XT (3M Unitek), Bisco ORTHO (Bisco), Light Bond (Reliance), and Quick Cure (Reliance) were prepared, and the samples were extracted in 3 mL of BME (Basal Medium Eagle) with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in a 96-well dish and maintained in a humidified incubator for 24 hours at 37 degrees C, 5% CO(2), and 95% air. After 24 hours of incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then, L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyl tetrazolium (MTT) test. Twelve wells were used for each specimen, and the MTT tests were applied two times. The data were statistically analyzed by one-way analysis of variance (ANOVA) and Tukey HSD tests. RESULTS: Results with L929 fibroblasts demonstrated that except for Transbond XT, freshly prepared composite materials did not reduce vital cell numbers (P > .05) compared with the control group. Our data demonstrate that Transbond XT showed significant cytotoxicity compared with the control group. CONCLUSION: Results indicate that tested orthodontic bonding composites are suitable for clinical application, but that further studies using different test methods are needed for Transbond XT.Öğe The effects of hemostatic agents to color change of dental tissue(2012) Yalçin M.; Karaarslan E.Ş.; Umar I.; Cebe M.A.Objectives: The aim of this study was to investigate the effects of hemostatic agents to the color change of dental tissues. Materials and Methods: 40 extracted human third molars which are not affected by caries used in this study. Occlusal surfaces removed to obtain flat dentinal surfaces and teeth mounted acrylic. Specimens were divided into four groups of 10 to be tested four different hemostatic agents. Prepared tooth groups were aplied hemostatik agent (Hemoban, Sultan®, USA; Hemospad, Dentsply, Germany; Ankafert Blood Stopper©, Ankafert, Turkey; Hemostatic solution, WP dental, Germany). Before and after application of hemostatic agents to flat surface of teeth, L*a*b* values were measured with a colorimeter (Easyshade® Compact, VITA, Germany), and ?E*ab values were calculated. Data were compared using Kruskall-Wallis H ve Mann-Whitney U tests (p=0.05). Results: The highest color change were found with Hemoban hemostatic solution. The lowest color change was observed with Hemospad hemostatic agent (p<0.05). There were statistically significant differences amoung groups according to color change (p<0.05). Conclusions: As a result, hemostatic agents used in dentistry as a hemostatic can cause color change over the dentine tissue. Therefore, when solutions which are used as a hemostatic agent are used, should be careful to avoid contact these solutions with the open dentine tissue.Öğe Endodontic treatment of a maxillary first molar having three mesiobuccal canals: A case report(Cumhuriyet University Faculty of Dentistry, 2015) Keleş A.; Yalçin M.Aim: The objective of this case report is to present a successful root canal treatment of a maxillary first molar having three mesiobuccal canals with separate orifices. Case presentation: An 18 year old female patient was admitted to our clinic, complaining from severe pain. We observed during the clinical examination that root canal treatment of the maxillary right first molar was already initiated. The tooth was percussion sensitive, cold and heat sensitive, although there was no referred pain. A broken endodontic instrument in the disto-buccal root canal was found during the radiographic examination. After extensive clinical and radiographic examination tooth was prepared for nonsurgical endodontic treatment. Starting the endodontic treatment, it was observed the presence of two canals orifices in the buccomesial root with no preparation. Though we failed to remove the fracture for the endodontic instrument, the instrument was bypassed. Root canals were obturated with guttapercha and sealer. The tooth was restored with composite resin. Conclusion: Recall examination as far as 6 months post-treatment found no sensitivity to percussion or palpation and recall radiography after treatment show no evidence of any periradicular lesion.Öğe Heat conduciton properties of flowable composite resins(Wolters Kluwer Medknow Publications, 2016) Yalçin M.; Keleş A.; Şişman R.; Karagöz S.Objectives: To investigate and compare heat conduction of different flowable composites. Materials and Methods: In this study, four different flowable composites; GC Gradia Direct LoFlo (GC Corporation, Tokyo, Japan), Filtek Ultimate (3M ESPE, St. Paul, USA), Grandio Flow (VOCO GmbH, Cuxhaven, Germany) and SDI Wave (SDI, Victoria, Australia) were used. Flowable composites were placed into standard molds and used according to manufacturer instructions. The samples were prepared for every brand of flowable composites. The Heat Conduction Unit's (P. A. Hilton Ltd., England) linear heat conduction module was used in determining the flowable composites heat conductivity. The data were statistically analyzed by Mann-Whitney U-test (SPSS 13.0, SPSS, Chicago, IL, USA). Results: Heat conduction values of flowable composites were found different each other. Results for GC Gradia Direct and Grandio Flow were significantly different from 3M ESPE and SDI (P < 0.05). However, result for 3M ESPE was and nonsignificant different from SDI (P > 0.005). Conclusions: Within the limits of this study, flowable composites transmit the heat. However, results for GC Gradia Direct and Grandio Flow were significantly different from 3M ESPE and SDI.Öğe Influence of caries detection dye on composites bond strength of carious affected dentin(Cumhuriyet University Faculty of Dentistry, 2014) Yalçin M.; Cebe F.; Cebe M.A.; Dündar A.; Öztürk B.; Şengun A.Objectives: The aim of this in vitro study was to evaluate effect of three different caries detecting dyes on shear bond strength of a self etch adhesive to caries affected dentin. Materials and Methods: 60 extracted human third molars which are affected by caries used in this study. Occlusal surfaces removed to obtain flat dentinal surfaces and teeth mounted acrylic. Specimens were divided into four groups of 15 to be tested three different caries detecting dyes. First group is control and others Caries Detector, Quadrant Cari Test, Sable Seek groups. In control group decayed dentine tissues remowed with silicon carbite papers (300 gride) and in others groups caries detectors applied and carious dentine tissues remowed with silicon carbite papers. Than dentin bonding systems Clearfil SE Bond applied and specimens restorated with composite resin. All products were used according to manufacturer's instructions. Bond strengths were tested and data were statistically analyzed one-way Analysis of Variance (ANOVA) and Tukey tests. Results: As results of this study, Quadrant Cari Test group showed lower bond strength than other detecting dyes groups. While bond strengths of Caries Detector and Sable Seek groups were similar, their bond strengths were significantly higher than Quadrant Cari Test group (p<0,05). Conclusions: According to the results of this in vitro study caries detecting dyes may lead to increased shear bond strength values obtained.
