Cytotoxic effects of orthodontic composites.

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Tarih

2010

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

OBJECTIVES: To evaluate the cytotoxic effects of five different light-cured orthodontic bonding composites. Materials and Methods: The orthodontic composites Heliosit Orthodontic (Ivoclar), Transbond XT (3M Unitek), Bisco ORTHO (Bisco), Light Bond (Reliance), and Quick Cure (Reliance) were prepared, and the samples were extracted in 3 mL of BME (Basal Medium Eagle) with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in a 96-well dish and maintained in a humidified incubator for 24 hours at 37 degrees C, 5% CO(2), and 95% air. After 24 hours of incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then, L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyl tetrazolium (MTT) test. Twelve wells were used for each specimen, and the MTT tests were applied two times. The data were statistically analyzed by one-way analysis of variance (ANOVA) and Tukey HSD tests. RESULTS: Results with L929 fibroblasts demonstrated that except for Transbond XT, freshly prepared composite materials did not reduce vital cell numbers (P > .05) compared with the control group. Our data demonstrate that Transbond XT showed significant cytotoxicity compared with the control group. CONCLUSION: Results indicate that tested orthodontic bonding composites are suitable for clinical application, but that further studies using different test methods are needed for Transbond XT.

Açıklama

Anahtar Kelimeler

resin cement, Transbond XT, analysis of variance, animal, article, cell shape, cell survival, dental bonding, drug effect, L cell, mitochondrion, mouse, nonparametric test, orthodontic device, Analysis of Variance, Animals, Cell Shape, Cell Survival, Dental Bonding, Light-Cured, L Cells (Cell Line), Mice, Mitochondria, Orthodontic Brackets, Resin Cements, Statistics, Nonparametric

Kaynak

The Angle orthodontist

WoS Q Değeri

Scopus Q Değeri

N/A

Cilt

80

Sayı

4

Künye