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Öğe Antimicrobial effects of several calcium silicate-based root-end filling materials(Japanese Soc Dental Materials Devices, 2014) Damlar, Ibrahim; Ozcan, Erhan; Yula, Erkan; Yalcin, Muhammet; Celik, SalihThe purpose of this study was to evaluate, in vitro, the antimicrobial effect of iRoot BP, iRoot BP Plus, and mineral trioxide aggregate (MTA) against Entercoccus faecalis and Candida albicans by using direct contact test. The materials were tested immediately after application to the microtiter wells and after setting for 1-day and for 7-days. Ten microliters of microbial suspension was added to each well for direct contact with each material for 1 h at 37 degrees C and 100% humidity. Then fresh media was added and, survival of bacteria and fungi was determined by using 10-fold serial dilution and inoculated onto agar plates. In fresh and 1-day samples all of tested materials showed statistically significant antimicrobial effects compared to control groups (p<0.05). In 7-day samples, there were no significantly differences compared to control groups. MTA, iRoot BP and iRoot BP Plus had similar antimicrobial efficacy against E. faecalis and C. albicans.Öğe Cement Thickness of Inlay Restorations Made of Lithium Disilicate, Polymer-Infiltrated Ceramic and Nano-Ceramic CAD/CAM Materials Evaluated Using 3D X-Ray Micro-Computed Tomography(Wiley, 2018) Uzgur, Recep; Ercan, Ertugrul; Uzgur, Zeynep; Colak, Hakan; Yalcin, Muhammet; Ozcan, MutluPurposeTo evaluate the marginal and internal cement thicknesses of inlay restorations made of various CAD/CAM materials using 3D X-ray micro-computed tomography (micro-CT) technique. Materials and MethodsCaries-free extracted mandibular molars (N = 30) with similar size were randomly assigned to three groups (N = 10 per group). Mesio-occlusal-distal (MOD) cavities were prepared, and inlay restorations were obtained by milling out CAD/CAM materials namely, (a) IPS: monolithic lithium disilicate (control), (b) VE: polymer-infiltrated ceramic, and (c) CS: nano-ceramic using a CAM unit. Marginal and internal cement thicknesses were measured using 3D micro-CT. Data were analyzed using 1-way ANOVA and Tukey's tests (alpha = 0.05). ResultsThe mean marginal and internal cement thickness were not significant in all inlay materials (p > 0.05). Mean marginal cement thickness (m) was the lowest for the IPS group (67.54 10.16) followed by VE (84.09 3.94) and CS (95.18 +/- 10.58) (p > 0.05). The internal cement thickness (m) was the lowest in the CS group (54.85 +/- 6.94) followed by IPS (60.58 +/- 9.22) and VE (77.53 +/- 12.13) (p > 0.05). ConclusionMarginal and internal cement thicknesses of MOD inlays made of monolithic lithium disilicate, polymer-infiltrated ceramic, and nano-ceramic CAD/CAM materials were similar and all less than 100 m, which could be considered clinically acceptable. Clinical significanceMOD inlays made of different CAD/CAM materials presented similar cement thickness, less than 100 m.Öğe Cytotoxic effects of bulk fill composite resins on human dental pulp stem cells(Nihon Univ, School Dentistry, 2016) Sisman, Reyhan; Aksoy, Ayca; Yalcin, Muhammet; Karaoz, ErdalFive bulk fill composite resins, including SDR, Tetric EvoCeram Bulk Fill (TEC), X-trafil (XTF), Sonic Fill (SF), Filtek Bulk Fill (FBF), were used in this study. Human dental pulp stem cells were cultured in 12-well culture dishes (3 x 104 cells per cm(2)) and stored in an incubator at 37 degrees C and 5% CO2 for 1 day. On days 1, 7, 14, and 21 of co-culture, viable cells were measured using a WST-1 assay. Lower cell viability was observed with XTF and SDR bulk fill composite resins compared to the control group during the WST-1 assay. Although bulk fill composite resins provide advantages in practical applications, they are limited by their cytotoxic properties.Öğe Cytotoxic effects of orthodontic composites(E H Angle Education Research Foundation, Inc, 2010) Malkoc, Siddik; Corekci, Bayram; Ulker, Hayriye Esra; Yalcin, Muhammet; Sengun, AbdulkadirObjectives: To evaluate the cytotoxic effects of five different light-cured orthodontic bonding composites. Materials and Methods: The orthodontic composites Heliosit Orthodontic (Ivoclar), Transbond XT (3M Unitek), Bisco ORTHO (Bisco), Light Bond (Reliance), and Quick Cure (Reliance) were prepared, and the samples were extracted in 3 mL of BME (Basal Medium Eagle) with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in a 96-well dish and maintained in a humidified incubator for 24 hours at 37 degrees C, 5% CO(2), and 95% air. After 24 hours of incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then, L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyl tetrazolium (MTT) test. Twelve wells were used for each specimen, and the MTT tests were applied two times. The data were statistically analyzed by one-way analysis of variance (ANOVA) and Tukey HSD tests. Results: Results with L929 fibroblasts demonstrated that except for Transbond XT, freshly prepared composite materials did not reduce vital cell numbers (P>.05) compared with the control group. Our data demonstrate that Transbond XT showed significant cytotoxicity compared with the control group. Conclusion: Results indicate that tested orthodontic bonding composites are suitable for clinical application, but that further studies using different test methods are needed for Transbond XT. (Angle Orthod. 2010;80:759-764.)Öğe Cytotoxic effects of resin-modified orthodontic band adhesives Are they safe?(E H Angle Education Research Foundation, Inc, 2010) Malkoc, Siddik; Corekci, Bayram; Botsali, Hayriye Esra; Yalcin, Muhammet; Sengun, AbduelkadirObjective: To evaluate the cytotoxic effects of three different resin-modified orthodontic band adhesives. Materials and Methods: Three resin-modified orthodontic band adhesives (Bisco Ortho Band Paste LC (TM), Multi-Cure Glass lonomer Band Cement (TM), and Transbond Plus Light Cure Band Adhesive (TM)) were prepared and the samples were extracted in 3 mL of Basal Medium Eagle with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in wells of 96-well dishes and maintained in a humidified incubator for 24 hours at 37 degrees C, 5% CO(2), and 95% air. After 24-hour incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyltetrazolium test. Twelve wells were used for each specimen, and methyltetrazolium tests were applied two times. The data were statistically analyzed using one-way analysis of variance and Tukey Honestly Significantly Different tests. Results: Results with L929 fibroblasts demonstrated that all freshly prepared resin-modified orthodontic band adhesive materials reduced vital cell numbers (P > .05), in comparison to the control group. Our data demonstrate that all materials showed significant cytotoxicity compared to the control group. Conclusions: The results indicate that all materials showed significant cytotoxicity compared to the control group, and further studies using different test methods are needed for all resin-modified orthodontic band adhesives. (Angle Orthod. 2010;80:890-895.)Öğe Cytotoxicity evaluation of dentin bonding agents by dentin barrier test on 3-dimensional pulp cells(Mosby-Elsevier, 2011) Sengun, Abdulkadir; Yalcin, Muhammet; Ulker, Hayriye Esra; Ozturk, Bora; Hakki, Sema S.Objective. The aim of this study was to evaluate the effects of 4 dentin-bonding agents on the cell viability of bovine derived cells. Study design. Cytotoxicity of dentin-bonding agents (G-Bond [GB], Adper Prompt Self-Etch [APSE], Clearfil DC Bond System [CDCB], and Quadrant University-1-Bond [UB]) was analyzed with a dentin barrier test device using 3-dimensional (3D) pulp cell cultures. A commercially available cell culture perfusion chamber was separated into 2 compartments using a 500 mu m dentin disk. The 3D cultures were placed on a dentin disk and held in place with a special biocompatible stainless steel holder. Test materials were introduced into the upper compartment in direct contact with the cavity side of the dentin disks according to the manufacturer's instructions. Subsequently, the pulpal part of the perfusion chamber containing the cell cultures was perfused with a medium (2 mL/h). After an exposure period of 24 hours, cell survival was determined by using the MTT assay. Statistical analyses were performed using the Mann-Whitney U test. Results. In the dentin barrier test, cell survival rates of UB and CDCB were similar to the control group (P > .05). However, all other tested materials were cytotoxic for the 3D pulp-derived cell cultures (P > .05). Conclusions. Dentin-bonding agents include biologically active ingredients and may modify pulp cell metabolism when the materials are used in deep cavities in spite of a dentin barrier. If these adhesive agents are used in deep cavities, a biocompatible cavity liner should be used. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011; 112: e83-e88)Öğe Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells(Hindawi Ltd, 2013) Ulker, Hayriye Esra; Ulker, Mustafa; Gumus, Hasan Onder; Yalcin, Muhammet; Sengun, AbdulkadirThis study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary luting cements. For cytotoxicity testing, bovine pulp-derived cells transfected with Simian virus 40 Large T antigen were exposed to extracts of eugenol-containing (Rely X Temp E) and eugenol-free (Provicol, PreVISION CEM, and Rely X Temp NE) temporary luting cements for 24 h. The cytotoxicity of the same materials was also evaluated in a dentin barrier test device using three-dimensional cell cultures of bovine pulp-derived cells. The results of the cytotoxicity studies with two-dimensional cultures of bovine dental pulp-derived cells revealed that cell survival with the extracts of Rely X Temp E, Provicol, PreVISION CEM, and Rely X Temp NE was 89.1%, 84.9%, 92.3%, and 66.8%, respectively. Rely X Temp NE and Provicol showed cytotoxic effects on bovine dental pulp-derived cells (P < 0.05). The results of the dentin barrier test revealed that cell survival with the above-mentioned temporary cement was 101.5%, 91.9%, 93.5%, and 90.6%, respectively. None of the temporary luting cements significantly reduced cell survival compared with the negative control in the dentin barrier test (P > 0.05). Biologically active materials released from temporary luting cements may not influence the dentine-pulp complex if the residual dentine layer is at least 0.5 mm thick.Öğe Evaluation of a collagen-bioaggregate composite scaffold in the repair of sheep pulp tissue(Istanbul Univ Press, Istanbul Univ Rectorate, 2021) Daya, Burak; Bilecen, Deniz Sezlev; Eroksuz, Hatice; Yalcin, Muhammet; Hasarca, VasafPurpose This study aimed to compare the effects of the collagen-BioAggregate mixture (CBA-M) and collagen-BioAggregate composite (CBA-C) sponge as a scaffolding material on the reparative dentin formation. Materials and Methods CBA-C sponge (10:1 w/w) was obtained and characterized by Scanning Electron Microscopy (SEM) and Mercury Porosimetry. Cytotoxicity of the CBA-C sponge was tested by using the L929 mouse fibroblast cell line. Dental pulp stem cells (DPSCs) were isolated from the pulp tissue of sheep teeth and characterized by flow cytometry for the presence of mesenchymal stem cell marker, CD44. The osteogenic differentiation capability of isolated DPSCs was studied by Alizarin Red staining. The cells were then used to study for the compatibility of CBA-C sponge with cell proliferation and calcium phosphate deposition. The effect of CBA-C sponge and CBA-M on the induction of dentin regeneration was studied in the perforated teeth of sheep for the eight-week period. All the analyses were performed with appropriate statistical hypothesis tests. Results CBA-C sponge was found to be biocompatible for DPSCs. The DPSCs seeded on the CBA-C sponge were able to differentiate into the osteoblastic lineage and deposit calcium phosphate crystals in vitro. Reparative dentin formation was observed after the second week in the CBA- C sponge applied group. At the end of eight weeks, a complete reparative dentin structure was formed in the CBA-C sponge applied group, whereas necrotic tissue residues were observed in groups treated with the CBA-M. Conclusion CBA-C sponge represents a better microenvironment for reparative dentin formation probably due to maintaining DPSCs and allowing their osteogenic differentiation and thus calcium phosphate deposition.Öğe Evaluation on Shear Bond Strength of Different Glass Ionomer and Hydroxy Apatite Cements Used in Ossiculoplasty(AVES,, 2015-01) Kalcioglu, M. Tayyar; Uzun, Ismail Hakki; Yalcin, Muhammet; Malkoc, Meral Arslan; Öğreten, Ayse Tuba; Hanege, Fatih MehmetBackground: Glass ionomer cements (GIC) have been widely used in dentistry for many years. In recent years, GIC have also been used for ossiculoplasty. The bond strength of GIC used in ossiculoplasty and the way they may change over the years in the cementation area are being questioned. The bonding strength of the substance may be of importance for long-term outcomes. Aims: The aim of this study was to investigate the bond strength of different GIC on ossicles. Study Design: In vitro study. Methods: Twenty ossicles were obtained from patients who had undergone ear surgery. All specimens were randomly divided into four subgroups. All specimens were inserted into a specially designed apparatus for shear bond strength (SBS) testing. The tested materials [Aqua Meron (AM), Aqua Cem (AC), Ketac Cem (KC), and Otomimix CPB (OH)] were prepared and applied according to the manufacturer's instructions. The SBS was tested using a universal testing machine at a crosshead speed of 0.5 mm/min. Results: The mean SBSs were found to be 13.28 MPa, 23.43 MPa, 8.51MPa, and 1.78 MPa for AM, AC, KC, and OH, respectively. AC had the highest SBS, which was statistically significantly different from that of KC and OH (p<0.05). Both AM and KC had higher SBS than OH (p<0.05). Conclusion: The results obtained in this study by investigating the bone-bonding strength of cements widely used in ossiculoplasty demonstrate that some of these substances have a greater ability to bond to ossicles compared to others. Further clinical investigations are needed to test different parameters.Öğe Examination of surface porosity of current pulp capping materials by micro-computed tomography (micro-CT) method(Mre Press, 2024) Dayi, Burak; Yalcin, MuhammetWhen dental pulp is exposed, it must be covered with a biocompatible material to form reparative dentine. The material used, besides being biocompatible, should have an ideal surface structure for the attachment, proliferation and differentiation of dental pulp stem cells. This study aimed to evaluate the porosity of the microstructures of four pulp capping materials using micro-computed tomography (micro-CT). Biodentine, Bioaggregate, TheraCal and Dycal materials were prepared according to the manufacturer's instructions using 2 x 9 mm Teflon molds. A total of 60 samples, 15 in each group, were scanned using micro-CT. Open and closed pores and the total porosity of the microstructures of the materials were assessed. The findings obtained from the study were analyzed via the Kruskal-Wallis test followed by the Mann-Whitney U test. The porosity of Bioaggregate was significantly higher than that of Biodentine, Dycal and TheraCal in all porosity values. While Biodentine did not show a statistically significant difference in open and total porosity values from either TheraCal or Dycal, closed porosity values of Dycal were significantly higher than those of Biodentine and TheraCal. Because of the affinity of cells to porous surfaces, the pulp capping materials' microstructure may affect the pulp capping treatment's success. From this perspective, the use of Bioaggregate in direct pulp capping may increase the success of treatment.Öğe Heat conductive properties of set root canal sealers(Informa Healthcare, 2013) Keles, Ali; Ahmetoglu, Fuat; Simsek, Neslihan; Yalcin, Muhammet; Ocak, Mevlut SinanObjective. The aim of this study was to examine the thermal conductivity of five different root canal sealers in vitro. Materials and methods. Sealapex, AH Plus, AH 26, Endomethasone and RoekoSeal root canal sealers were examined. These materials were prepared in accordance with the manufacturer's instructions and applied to standard molds. Three samples of each material were prepared. The samples were kept for 5 days under 37 degrees C conditions. Measurements were taken using a heat conduction unit (P. A. Hilton Ltd. Stockbridge, Hants, UK). The thermal conductivity coefficient was calculated for each sample using the Fourier equation. Coefficients were analyzed statistically by the Kruskal-Wallis test. Results. Significant differences were found for thermal conductivity between some materials (p < 0.05). The conductivity coefficient of AH Plus was found to be higher than those of the other materials (p < 0.05). No statistically significant differences were found between AH 26 and RoekoSeal (p > 0.05) and the conductivity coefficients of these sealers were found to be lower than those of the other materials (p < 0.05). No statistically significant differences were found between Sealapex and Endomethasone (p > 0.05) and the conductivity coefficients of these sealers were found to be lower than that of AH Plus but higher than those of RoekoSeal and AH26 (p < 0.05). Conclusions. The results showed that root canal sealers functioned as thermal insulators and had different heat-conductive properties that depended on their composition.Öğe Long-Term Antibacterial Effects of Different Orthodontic Cements(Aves, 2011) Ozturk, Firat; Yalcin, Muhammet; Arslan, Ugur; Nalcaci, Ruhi; Aksakalli, SertacThe purpose of this study was to evaluate the antibacterial properties of different orthodontic cements using a direct contact test (DCT). Materials and Methods: The antibacterial properties of 6 orthodontic cements were evaluated with DCT. For the DCT, wells (n=8) of 96-microtiter plates were coated with the tested cements (Durelon, Ketac-Cem Radiopaque, GC Fuji Ortho Band LC Paste Pak, Meron, Ultra Band Lok, and 3M Unitek Multi Cure). A Streptococcus mutans suspension was placed on the surface of each specimen for 1 hour at 37 degrees C. Bacterial growth was monitored for 16 hours with a temperature-controlled microplate spectropho-tometer. The kinetics of the outgrowth in each well was recorded continuously at 650 nm every 30 minutes. Additional experiments were performed in which the tested materials were aged for 1 week, 1 month and 3 months. The data were analyzed by one-way ANOVA, and Tamhane's T2 multiple comparison test. Results: The results of the DCT showed that, freshly mixed samples of 3M Multi Cure (P=0.02), GC Fuji Ortho (P=0.04), Meron (P=0.001), and Durelon (P=0.001) showed antibacterial activity. After aging for one week 3M Unitek Multi Cure (P=0.000), Durelon (P=0.03) still showed antibacterial activity. In the experiments with 1 month aged specimens, only Durelon (P=0.001) showed antibacterial activity. In the 3 months aged specimens, 3M Multi Cure (P=0.000), GC Fuji Ortho (P=0.001), and Durelon (P=0.001) showed antibacterial property. Conclusion: Within the limitations of this study, 3M Unitek Multi Cure, GC Fuji Ortho, and Durelon, showed antibacterial properties during study period.
