Cytotoxicity evaluation of dentin bonding agents by dentin barrier test on 3-dimensional pulp cells
Küçük Resim Yok
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mosby-Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective. The aim of this study was to evaluate the effects of 4 dentin-bonding agents on the cell viability of bovine derived cells. Study design. Cytotoxicity of dentin-bonding agents (G-Bond [GB], Adper Prompt Self-Etch [APSE], Clearfil DC Bond System [CDCB], and Quadrant University-1-Bond [UB]) was analyzed with a dentin barrier test device using 3-dimensional (3D) pulp cell cultures. A commercially available cell culture perfusion chamber was separated into 2 compartments using a 500 mu m dentin disk. The 3D cultures were placed on a dentin disk and held in place with a special biocompatible stainless steel holder. Test materials were introduced into the upper compartment in direct contact with the cavity side of the dentin disks according to the manufacturer's instructions. Subsequently, the pulpal part of the perfusion chamber containing the cell cultures was perfused with a medium (2 mL/h). After an exposure period of 24 hours, cell survival was determined by using the MTT assay. Statistical analyses were performed using the Mann-Whitney U test. Results. In the dentin barrier test, cell survival rates of UB and CDCB were similar to the control group (P > .05). However, all other tested materials were cytotoxic for the 3D pulp-derived cell cultures (P > .05). Conclusions. Dentin-bonding agents include biologically active ingredients and may modify pulp cell metabolism when the materials are used in deep cavities in spite of a dentin barrier. If these adhesive agents are used in deep cavities, a biocompatible cavity liner should be used. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011; 112: e83-e88)
Açıklama
Anahtar Kelimeler
In-Vitro, Permeability, Monomers, Diffusion, Cultures
Kaynak
Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology
WoS Q Değeri
Q2
Scopus Q Değeri
N/A
Cilt
112
Sayı
3
