Cytotoxicity evaluation of dentin bonding agents by dentin barrier test on 3-dimensional pulp cells

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dc.authoridHakkı, Sema S/0000-0001-8665-6235
dc.authoridULKER, Hayriye Esra/0000-0002-2967-5680
dc.authorwosidHakkı, Sema S/Y-2878-2018
dc.contributor.authorSengun, Abdulkadir
dc.contributor.authorYalcin, Muhammet
dc.contributor.authorUlker, Hayriye Esra
dc.contributor.authorOzturk, Bora
dc.contributor.authorHakki, Sema S.
dc.date.accessioned2024-08-04T20:35:32Z
dc.date.available2024-08-04T20:35:32Z
dc.date.issued2011
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjective. The aim of this study was to evaluate the effects of 4 dentin-bonding agents on the cell viability of bovine derived cells. Study design. Cytotoxicity of dentin-bonding agents (G-Bond [GB], Adper Prompt Self-Etch [APSE], Clearfil DC Bond System [CDCB], and Quadrant University-1-Bond [UB]) was analyzed with a dentin barrier test device using 3-dimensional (3D) pulp cell cultures. A commercially available cell culture perfusion chamber was separated into 2 compartments using a 500 mu m dentin disk. The 3D cultures were placed on a dentin disk and held in place with a special biocompatible stainless steel holder. Test materials were introduced into the upper compartment in direct contact with the cavity side of the dentin disks according to the manufacturer's instructions. Subsequently, the pulpal part of the perfusion chamber containing the cell cultures was perfused with a medium (2 mL/h). After an exposure period of 24 hours, cell survival was determined by using the MTT assay. Statistical analyses were performed using the Mann-Whitney U test. Results. In the dentin barrier test, cell survival rates of UB and CDCB were similar to the control group (P > .05). However, all other tested materials were cytotoxic for the 3D pulp-derived cell cultures (P > .05). Conclusions. Dentin-bonding agents include biologically active ingredients and may modify pulp cell metabolism when the materials are used in deep cavities in spite of a dentin barrier. If these adhesive agents are used in deep cavities, a biocompatible cavity liner should be used. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011; 112: e83-e88)en_US
dc.identifier.doi10.1016/j.tripleo.2011.02.023
dc.identifier.endpageE88en_US
dc.identifier.issn1079-2104
dc.identifier.issn1528-395X
dc.identifier.issue3en_US
dc.identifier.pmid21546285en_US
dc.identifier.scopus2-s2.0-80051549948en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpageE83en_US
dc.identifier.urihttps://doi.org/10.1016/j.tripleo.2011.02.023
dc.identifier.urihttps://hdl.handle.net/11616/95403
dc.identifier.volume112en_US
dc.identifier.wosWOS:000293626600010en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMosby-Elsevieren_US
dc.relation.ispartofOral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIn-Vitroen_US
dc.subjectPermeabilityen_US
dc.subjectMonomersen_US
dc.subjectDiffusionen_US
dc.subjectCulturesen_US
dc.titleCytotoxicity evaluation of dentin bonding agents by dentin barrier test on 3-dimensional pulp cellsen_US
dc.typeArticleen_US

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