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Öğe 3R, 3?R zeaxanthin Protects Retina from Photo-Oxidative Damage: in vivo model(Wiley, 2017) Sahin, Nurhan; Akdemir, Fatih; Orhan, Cemal; Tuzcu, Mehmet; Yilmaz, Ismet; Juturu, Vijaya[Abstract Not Available]Öğe Antioxidant properties of different extracts of black mulberry (Morus nigra L.)(Tubitak Scientific & Technological Research Council Turkey, 2011) Kutlu, Turkan; Durmaz, Gokhan; Ates, Burhan; Yilmaz, Ismet; Cetin, M. SevketIn vitro antioxidant properties of 3 different extracts of black mulberry (Morus nigra L.) were investigated. Acidified methanol, acidified water, and non-acidified methanol/water solutions were used to prepare extracts. Different solvents caused different protonation on black mulberry anthocyanin structures, which were predicted according to their UV-VIS spectrum. Extracts indicated 2 main peaks at about similar to 280 and similar to 520 nm with different peak areas in their UV-VIS spectrum. The rank of extracts' total phenolic content and reducing power values were both found to be in correlation with their absorbance at similar to 280 nm. Meanwhile the same relationship was observed between DPPH scavenging activity and absorbance values at similar to 520 nm. Acidified extract of black mulberry was higher in beta-carotene prevention and DPPH radical scavenging activity than non-acidified extract. However, non-acidified extract represented a higher reducing power and metal chelating activity, and a higher content of total phenolics.Öğe Antioxidative effects of novel synthetic organoselenium compound in rat lung and kidney(Academic Press Inc Elsevier Science, 2009) Talas, Zeliha Selamoglu; Ozdemir, Ilknur; Yilmaz, Ismet; Gok, YetkinThe effects of environmental chemicals, drugs, and physical agents on the developing lung and kidney are influenced by the state of development and maturation. Selenium is an essential element with physiological nonenzymatic antioxidant properties. Therefore, we undertook the present study to evaluate the antioxidant potential of the novel synthetic organoselenium compounds (Se I and Se II). In this study, adult female Wistar rats were treated with DMBA and the novel organoselenium compounds [1-isopropyl-3-methylbenzimidazole-2-selenone (Se I) and 1,3-di-p-methoxybenzylpyrimidine-2-selenone (Se II)] in the determined doses. The protective effects of novel synthetic organoselenium compounds (Se I and Se II) against DMBA-induced changes in levels of some [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) activities and total glutathione (GSH), malonedialdehyde (MDA)] parameters in rat lung and kidney were investigated. As a result, it was found that both Se I and Se II had provided the antioxidant effects against DMBA-induced oxidative stress in rat lung and kidney and lipid peroxidation had also been decreased by these organoselenium compounds. (c) 2007 Elsevier Inc. All rights reserved.Öğe Antioxidative role of selenium against the toxic effect of heavy metals (Cd+2 Cr+3 on liver of rainbow trout (Oncorhynchus mykiss Walbaum 1792)(Springer, 2008) Talas, Zeliha Selamoglu; Orun, Ibrahim; Ozdemir, Ilknur; Erdogan, Kenan; Alkan, Aysel; Yilmaz, IsmetThe main purpose of this study is to discuss the effect of Cd+2, Cr+3 and Se metals on biochemical parameters in liver tissue of Oncorhynchus mykiss. The rainbow trout were exposed to heavy metal stress ( Cd+2, Cr+3) at 2 ppm dosage. The present study was undertaken to determine the protective effect of selenium treatment at the same dosage ( 2 ppm) on some biochemical parameters. The activity of catalase ( CAT), glutathione peroxidase ( GSH-Px), superoxide dismutase ( SOD) and the changes in levels of malondialdehyde ( MDA) from biochemical parameters were determined in liver tissue of the fish groups exposed to heavy metals, especially for the selenium-applied groups. Results of this study showed that the activities of CAT, GSH-Px and SOD in the tissues of fish exposed to the stress of Cd+2 and Cr+3 were significantly lower than the control groups ( P < 0.05). Meanwhile, the closer values to the control groups were obtained in selenium-added groups ( Cr+3 + Se+4, Cd+2 + Se+4). For the level of MDA, the last production of lipid peroxidation showed increases ( P < 0.05) in the groups exposed to the metal stress, whereas significant decreases were obtained in selenium-applied groups. The result of the statistical evaluation showed that the negative effects occurring in the biochemical parameters of the applied groups exposed to the toxicity of heavy metal were significantly eliminated ( P < 0.05) as a result of selenium treatment.Öğe The Bioequivalence Determination of Two Different Formulations of Enrofloxacin in Heifers Following Intramuscular Administration(Kafkas Univ, Veteriner Fakultesi Dergisi, 2010) Yilmaz, Ismet; Elmas, MuammerThe aim of this study was to evaluate the bioequivalence (BE) of two medicinal products of enrofloxacin, which have been also marketed as 10% injectable solution in Turkey, after the intramuscular injection (IM) at a single dose of 2.5 mg/kg of BW in the heifers. The present study was performed on healthy 6 Swiss-Brown (12-18 months and 340-400 kg BW) heifers. This study was carried out on the based a single dose cross-over design. Blood samples were taken into sterilized tubes just before, and 10(th), 20(th), 30(th), 45(th), 60(th) and 90(th) min. and 2(th), 3(th), 4(th), 6(th), 8(th), 12(th) and 24(th) h following injections. The plasma concentrations of enrofloxacin (ENR) were measured by high performance liquid chromatography (HPLC) following the extraction process. The plasma concentration-time curves for each animals showed that both products distributed according two-compartment open model. The basic pharmacokinetic parameters at this study were only the AUC(0-24) and AUC(total) were statistically significant (P<0.05) before logarithmic (log) transformation. Log transformed the AUC(0-24), AUC(total) and C(max) parameters and observed t(max) were used in BE evaluation. Minimum, maximum and mean AUC(0-24) AUC(total) and C(max) for A and B products were found in the acceptable ranges (70-143%). For the t(max) value log transformation has not been done and that were determined within the limits 80-125%. As a result; it is concluded that both products could be used instead of each other as an inter-changeable drugs.Öğe CHANGES IN TYROSINE HYDROXYLASE ACTIVITY, ADRENOMEDULLIN (ADM) AND TOTAL RNA LEVELS BY TREATMENT OF ORGANOSELENIUM COMPOUNDS IN RAT HYPOTHALAMUS EXPOSED TO 7,12-DIMETHYLBENZANTHRACENE (DMBA)(Parlar Scientific Publications (P S P), 2010) Ozdemir, Ilknur; Talas, Zeliha Selamoglu; Gok, Yetkin; Ates, Burhan; Yilmaz, IsmetThe effects of synthetic organoselenium compounds (Se I and Se II) on the activity of tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine synthesis as well as adrenomedullin (ADM) and total RNA levels were determined in the hypothalamus of adult rats exposed to DMBA (7,12-dimethylbenz[a]anthracene). DMBA, an organic environmental pollutant, is a polycyclic aromatic hydrocarbon that can induce a range of toxic effects and stress in rats. Selenium is an essential trace element, which interacts with antioxidants, and has anticancer and antihypertensive properties. TH is an aromatic amino acid hydroxylase whose activity is elevated in response to a range of stress inducers. TH activity is normally regulated by negative feedback in catecholamine biosynthesis. ADM is an abundantly present peptide in a broad range of normal tissues including adrenal medulla, lungs, kidneys and brain. Plasma ADM levels are elevated in a number of diseases including essential hyptertension and chronic renal failure. The antioxidant properties of ADM offer protection against organ damage induced by high blood pressure, ischemia and aging. DMBA treatment increased the TH activity and ADM level in the hypothalamus. These increases were found to be inhibited by Se I and Se II treatments. These studies demonstrate that synthetic organoselenium compounds can suppress DMBA-induced stress-related changes in the rat hypothalamus. Therefore, the antioxidant and antihypertensive effects of Se I and Se II may have important effects in the maintainance of homeostasis.Öğe ?-Cryptoxanthin ameliorates metabolic risk factors by regulating NF-?B and Nrf2 pathways in insulin resistance induced by high-fat diet in rodents(Pergamon-Elsevier Science Ltd, 2017) Sahin, Kazim; Orhan, Cemal; Akdemir, Fatih; Tuzcu, Mehmet; Sahin, Nurhan; Yilmaz, Ismet; Juturu, VijayaThe aim of this experiment was to determine the effects of beta-cryptoxanthin (BCX) on the cardiometabolic health risk factors and NF-kappa B and Nrf2 pathway in insulin resistance induced by high-fat diet (HFD) in rodents. Twenty-eight Sprague-Dawley rats were allocated into four groups: (1) Control, rats fed a standard diet for 12 weeks; (2) BCX, rats fed a standard diet and supplemented with BCX (2.5 mg/kg BW) for 12 weeks; (3) HFD, rats fed a HFD for 12 weeks, (4) HFD + BCX, rats fed a HFD and supplemented with BCX for 12 weeks. BCX reduced cardio-metabolic health markers and decreased inflammatory markers (P < 0.001). Rats fed a HFD had the lower total antioxidant capacity and antioxidant enzymes activities and higher MDA concentration than control rats (P < 0.001 for all). Comparing with the HFD group, BCX in combination with HFD inhibited liver NF-kappa B and TNF-alpha expression by 22% and 14% and enhanced liver Nrf2, HO-1, PPAR-alpha, and p-IRS-1 by 1.43,1.41, 3.53, and 1.33 fold, respectively (P < 0.001). Furthermore, in adipose tissue, BCX up-regulated Nrf2, HO-1, PPAR-alpha, and p-IRS-1 expression, whereas, down-regulated NF-kappa B and TNF-alpha expression. In conclusion, BCX decreased visceral fat and cardiometabolic health risk factors through modulating expressions of nuclear transcription factors. (C) 2017 Elsevier Ltd. All rights reserved.Öğe Cuscuta arvensis Beyr Dodder: In Vivo Hepatoprotective Effects Against Acetaminophen-Induced Hepatotoxicity in Rats(Mary Ann Liebert, Inc, 2018) Koca-Caliskan, Ufuk; Yilmaz, Ismet; Taslidere, Asli; Yalcin, Funda N.; Aka, Ceylan; Sekeroglu, NazimCuscuta arvensis Beyr. is a parasitic plant, and commonly known as dodder in Europe, in the United States, and tu si zi shu in China. It is one of the preferred spices used in sweet and savory dishes. Also, it is used as a folk medicine for the treatment particularly of liver problems, knee pains, and physiological hepatitis, which occur notably in newborns and their mothers in the southeastern part of Turkey. The purpose of this study was to investigate the hepatoprotective effects and antioxidant activities of aqueous and methanolic extracts of C. arvensis Beyr. on acetaminophen (APAP)-induced acute hepatotoxicity in rats. The results were supported by subsequent histopathological studies. The hepatoprotective activity of both the aqueous and methanolic extracts at an oral dose of 125 and 250mg/kg was investigated by observing the reduction levels or the activity of alkaline phosphatase, alkaline transaminase, aspartate aminotransferase, blood urine nitrogen, and total bilirubin content. In vivo antioxidant activity was determined by analyzing the serum superoxide dismutase, malondialdehyde, glutathione, and catalase levels. Chromatographic methods were used to isolate biologically active compounds from the extract, and spectroscopic methods were used for structure elucidation. Both the methanolic and aqueous extracts exerted noticable hepatoprotective and antioxidant effects supporting the folkloric usage of dodder. One of the bioactive compounds was kaempferol-3-O-rhamnoside, isolated and identified from the methanolic extract.Öğe Cytotoxic Effects of Cucurbitacin I and Ecballium elaterium on Breast Cancer Cells(Natural Products Inc, 2018) Yilmaz, Kadir; Karakus, Fuat; Eyol, Ergul; Tosun, Emir; Yilmaz, Ismet; Unuvar, SongulThe objective of this study was to investigate the inhibitory effect of cucurbitacin I (CuI) and Ecballium elaterium L. (fruit juice and chloroform extract) on breast cancer cells (MCF-7 and MDA-MB-231). The Cul content of E. elaterium fruit juice and chloroform extract was quantified using high performance liquid chromatography. The cytotoxic effects of the fruit juice, chloroform extract and CuI were determined by MTT, wound healing and colony formation assays; all had an anti-proliferative activity on the breast cancer cells. Clarifying the mechanisms of cucurbitacins will enable the identification of new molecular targets for breast cancer therapy.Öğe Determination of characterization, antibacterial and drug release properties of POSS- based film synthesized with sol-gel technique(Tubitak Scientific & Technological Research Council Turkey, 2021) Acari, Idil Karaca; Koytepe, Suleyman; Ates, Burhan; Yilmaz, Ismet; Seckin, Turgaythe study, antibacterial film synthesis was aimed using sol-gel technique from POSS structure with various functional groups. For this purpose, antibacterial properties have been acquired by metronidazole to the films to be synthesized. The films obtained were coated on glass surface samples by dip coating method. Antibacterial activities of surface coated glass samples were observed in E.coli and S. aureus bacteria. Metronidazole release studies in the film samples were followed by UV spectrophotometer. It was observed that drug release reached 68.90% at the end of the 24th h. As a result, it is thought that the synthesized film will be a good candidate especially for biomedical surface coating areas.Öğe The effectiveness of chitosan-mediated silencing of PDGF-B and PDGFR-? in the mesangial proliferative glomerulonephritis therapy(Academic Press Inc Elsevier Science, 2019) Alan, Saadet; Salva, Emine; Yilmaz, Ismet; Turan, Suna Ozbas; Akbuga, JulidePlatelet-derived growth factor-B (PDGF-B) is a growth factor that plays an important role in the progression of mesangial proliferative glomerulonephritis (MsPGN). PDGF-B may contribute to mesangioproliferative changes and is overexpressed in MsPGN. Recently, small interfering RNAs (siRNAs) have been widely used for gene silencing effects in experimental models of renal diseases. Nanoparticle-based therapeutics are preferred for reasons such as increasing therapeutic efficacy and reducing toxic effects caused by high doses. The distribution of nanoparticles to the kidney is a significant advantage in siRNA delivery. The aim of this study was to investigate the efficacy of chitosan/siRNA nanoplexes in silencing of PDGF-B and PDGFR-beta genes in kidney and to decrease mesangial cell proliferation and matrix accumulation in MsPGN model induced by anti-Thy-1.1 antibody. The therapeutic effects of chitosan/siPDGF-B + siPDGFR-beta nanoplexes in glomerulonephritic rats were studied by molecular, biochemical, and histopathologic evaluations. Chitosan/siPDGF-B + siPDGFR-beta nanoplexes markedly reduced PDGF-B and PDGFR-beta mRNA and protein expressions in experimental MsPGN model. Histopathologic examination results showed that the silencing of PDGF-B and its receptor PDGFR-beta led to reduction in mesangial cell proliferation and matrix accumulation. The use of chitosan/siPDGF-B + siPDGFR-beta nanoplexes for silencing the PDGF-B pathway in MsPGN can be considered as a new effective therapeutic strategy.Öğe The effects of apricot on serum proteins and liver enzymes in rats(Vup Food Research Inst, Bratislava, 2013) Yilmaz, Ismet; Temel, Ismail; Gursoy, Sule; Dogan, ZumrutThis study aimed to investigate the effects of different rates and feeding periods of sun-dried organic apricot (SDOA) supplementation on serum proteins and liver enzymes in rats. Numbers of 120 male and 120 female rats were randomly divided into five groups. The control group was fed with normal rat chow, and the others with 1%, 2.5%, 5% and 10% SDOA-supplemented diet, respectively. At the end of the 30th day of feeding periods, blood samples of 8 rats from each gender of every group were taken. Serum samples were used for measurements of albumin (ALB), total protein (TP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) levels. This procedure was repeated on the 60th and 120th days. Effects of rates and periods on parameters and interactions were investigated by two-way ANOVA. As the rates of SDOA in diet increased, decreases were observed in all parameters of males, and in ALP, AST and TP parameters of females. Considering periods, an effective role was observed on ALB, ALT and TP levels in genders. However, there were no significant interactions between rates and periods. The rate of 1% had beneficial effect on parameters in genders. However, the optimal period was not determined.Öğe Effects of larazotide acetate, a tight junction regulator, on the liver and intestinal damage in acute liver failure in rats(Sage Publications Ltd, 2021) Caliskan, Ali Riza; Gul, Mehmet; Yilmaz, Ismet; Otlu, Baris; Uremis, Nuray; Uremis, Muhammed Mehdi; Kilicaslan, IlkayBackground and Aim The epithelial cells are the strongest determinants of the physical intestinal barrier. Tight junctions (TJs) hold the epithelial cells together and allow for selective paracellular permeability. Larazotide acetate (LA) is a synthetic octapeptide that reduces TJ permeability by blocking zonulin receptors. In this study, we aimed to investigate the effects of LA, a TJ regulator, on the liver and intestinal histology in the model of acute liver failure (ALF) in rats. Materials and Methods The thioacetamide (TAA) group received intraperitoneal (ip) injections of 300 mg/kg TAA for 3 days. The TAA+LA(dw) (drinking water) group received prophylactic 0.01 mg/mL LA orally for 7 days before the first dose of TAA. The LA(dw) group received 0.01 mg/mL LA orally. The TAA + LA(g) (gavage) group received prophylactic 0.01 mg/mL LA via oral gavage for 7 days before the first dose of TAA. The LA(g) group received 0.01 mg/mL LA via oral gavage. While liver tissue was evaluated only with light microscopy, intestinal samples were examined with light and electron microscopy. Results Serum ammonia, AST, and ALT levels in the TAA group were significantly higher than in control groups (all p < 0.01). Serum ALT levels in the TAA + LA(dw) group were significantly lower than in the TAA group (p < 0.05). However, serum ammonia and ALT levels did not differ between the TAA and other groups. Serious liver damage in the TAA group was accompanied by marked intestinal damage. There was no significant difference between the TAA and TAA + LA(dw) groups and TAA and TAA + LA(g) groups for liver damage scores. However, intestinal damage scores significantly decreased in the TAA + LA(dw) group compared to the TAA group. In the TAA + LA(dw) group, fusion occurred between the surface epithelial cells of neighboring villi and connecting regions formed as epithelial bridges between the villi. Conclusion Our findings suggest that LA reduced intestinal damage by acting on TJs in the TAA-induced ALF model in rats.Öğe Effects of sodium selenite on some biochemical and hematological parameters of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) exposed to Pb2+ and Cu2+(Springer, 2008) Ates, Burhan; Orun, Ibrahim; Talas, Zeliha Selamoglu; Durmaz, Gokhan; Yilmaz, IsmetThis study was carried out to understand the preventive effect of selenium (Se4+) on heavy metal stress induced by lead and copper in rainbow trout (Oncorhynchus mykiss). Variation in glutathione peroxidase (Se-GSH-Px) and superoxide dismutase (SOD) activity, and in malondialdehyde (MDA) levels in liver, spleen, heart, and brain tissues of rainbow trout after 72 h of exposure to Pb2+ and Cu2+ were investigated in the presence and absence of Se4+. In the presence of Se4+, Se-GSH-Px activity and SOD activity were found to be higher and MDA levels were lower compared with in its absence. Hematological parameters were also determined and it has been observed that total leukocyte count (WBC), mean cell volume (MCV), and mean cell hemoglobin (MCH) were increased and erythrocyte number (RBC), hemoglobin (Hb), and hematocrit value (Hct; P < 0.05) were decreased in fish exposed to heavy metals in the absence of selenium. Selenium presence recovered hematological parameters to normal levels. In the light of our findings, it could be stated that Pb2+ and Cu2+ lead to dramatic changes in biochemical and hematological parameters and selenium caused these parameters to converge to control levels when it was administered concurrently with these heavy metals.Öğe Effects of sun dried organic apricot on serum total cholesterol, high density lipoprotein-cholesterol, triglyceride and total antioxidant capacity levels in rats(2020) Yilmaz, Ismet; Temel, Ismail; Aydemir, SongulAim: In this study, we aimed to investigate the effects of sun dried organic apricot (SDOA) consumption on serum Total Cholesterol (TC), High-Density Lipoprotein-Cholesterol (HDL-C), Triglyceride (TG) and Total Antioxidant Capacity (TAC) levels in ratsMaterial and Methods: 120 males, 120 females rats were divided in to four groups according to their SDOA rates (1%, 2.5, 5 and 10) in chow (to be 24 rats in each gruop), and feding periods. At 120 days periods, the control group was fed with standard rat chow and the others were fed with 1%, 2.5, 5 and 10 SDOA supplemented diet. At the end of 30th, 60th, 120th days; 8 rats which each genders/ groups were sacrified by diethyl ether inhalation anesthesia, app. 8-10 mL blood samples were taken by intracardiac puncture and obtained serum samples were maintained at -20 ° C until the day of analysis.Results: After statistical analysis, TC levels of male rats; HDL-C levels of female rats increased in paralel with the feding process. Both TC and HDL-C levels of female rats were significantly higher than those of males. Although there were a significant decreases in TG levels of both sexes at the end of feding periods, there was no significant difference between TG levels of both genders.Conclusion: It is thought that increases in TC and HDL-C levels are caused by rats affected by long-term feding periods. In addition, the significant difference between TC and HDL-C levels of male and female rats are thought to be affected by gender-specific reference values. The decrease in TG levels in both sexes is considered to be due to the increased energy needs of rats as well as the effect of SDOA-added feeds.Öğe The effects of synthetic organoselenium compounds on nitric oxide in DMBA - induced rat liver(Triveni Enterprises, 2009) Talas, Zeliha Selamoglu; Bayraktar, Nihayet; Ozdemir, Ilknur; Gok, Yetkin; Yilmaz, IsmetDMBA (7,12-dimethylbenz[a]anthracene) is known to generate DNA-reactive species during their metabolism, which may enhance oxidative stress in cells. Since selenium is known as a non-enzymic antioxidant, health problems induced by many environmental pollutants, have stimulated the evaluation of relative antioxidant potential of selenium and synthetic organoselenium compounds. Therefore, we aimed to evaluate chemopreventive potential of synthetic organoselenium compounds by monitoring level of liver nitric oxide. In this study, adult female Wistar rats were treated with DMBA and the novel organoselenium compounds (Se I) and (Se II) in the determined doses. DMBA-induced in rats, the effects of organoselenium compounds on nitric oxide levels in rat liver was studied. In this study, it has been observed a statistically significant increase in (Nitric Oxide) levels for the liver of rat exposed to DMBA (p<0.05). However with administration of Se I and Se II there was a statistically significant decrease in NO levels (p<0.05). The ability of the organoselenium compounds to prevent oxidative damage induced by DMBA in rat livers was rationalized. Protection against nitric oxide measured in Se I and Sell treated groups were provided by synthesized organoselenium compounds. Se I and Sell both provided chemoprevention against DMBA-induced oxidative stress in rat liver.Öğe Fabrication and characterization of high molecular mass tmpe-based polyurethane wound dressing materials containing allantoin and gentamicin by electrospinning(Eurasia Acad Publ Group (Eapg), 2023) Cakmen, Ayse Basak; Noma, Samir Abbas Ali; Gurses, Canbolat; Koytepea, Suleyman; Ates, Burhan; Yilmaz, IsmetIn this study, biocompatible, antibacterial and high mechanical strength polyurethane-based wound dressing materials were prepared by using the electrospinning technique. In addition, allantoin and gentamicin which will contribute to wound healing, were incorporated into these fiber materials. Polyurethane structures containing trimethylolpropane ethoxylate (TMPE) with 2 different molecular weights were synthesized. TMPE-based polyurethanes/polycaprolactone (1:3) blends were also prepared by adding 1% gentamicin and 10% allantoin and they were knitted by the electrospinning method and turned into a wound dressing material. After this stage, chemical structure, morphological, thermal and mechanical properties, flexibility, antibacterial effect, in vitro biocompatibility, cell adhesion tests, allantoin release level, and biodegradability of the prepared wound dressing materials were performed. The prepared fiber materials exhibited antibacterial properties and 80% cell viability, approximately. In addition, the obtained wound dressing materials showed high mechanical strength and ideal gas permeability. For this reason, it offers an ideal alternative for closing wounds.Öğe Hepatoprotective properties for Salvia cryptantha extract on carbon tetrachloride-induced liver injury(C M B Assoc, 2017) Yalcin, Alper; Yumrutas, Onder; Kuloglu, Tuncay; Elibol, Ebru; Parlar, Ali; Yilmaz, Ismet; Pehlivan, MustafaThe present study was designed to determine the possible hepatoprotective effects of Salvia cryptantha (black weed) plant extract against carbon tetrachloride (CCl4)-induced hepatic injury in rats. Animals were grouped as follows: control group (Group I), CCl4 group (Group II), olive oil group (Group III), CCl4 + S. cryphantha 200 mg/kg group (Group IV), and CCl4 + S. cryptantha 400 mg/kg group (Group V). Rats were injected intraperitoneally with CCl4 diluted in olive oil (50% v/v) at a dose of 1ml/kg body weight. Bax and Caspase3 were determined by immunohistochemical staining, while apoptotic index was evaluated using TUNEL assay. Total mRNA was isolated from liver tissues, and the levels of BCL2, Caspase3, SOD, CAT, and glutathione peroxidase (GPx) were determined by using PCR, while MDA level were determined using a colorimetric assay. The antioxidant and anti-apoptotic gene transcripts were decreased in all of the control and treatment groups, while Caspase3 levels were not statistically different. The S. cryptantha plant extract treatment was also found to improve SOD, GPx, and catalase levels, while reducing the serum levels of MDA. The extract of S. cryptantha supplementation had a protective effect against CCl4-induced liver damage. S. cryptantha extract as a supplement may be useful as a hepato-protective agent to combat the toxic effects caused by CCl4 and other chemicals.Öğe How do vegetable oils (hazelnut and canola) affect the reproductive system in male rats?(Pagepress Publ, 2018) Kati, Bulent; Oguz, Fatih; Yilmaz, Ismet; Akdemir, Ender; Altintas, Ramazan; Akpolat, Nusret; Taskapan, Mehmet CagatayObjective: Vegetable oils have an important place in our daily diet. This study starts from this point to investigate the effects of canola oil and hazelnut oil in the male reproductive system in rats. Material and methods: 30 male rats were used in this 16-week study. The animals were divided into three groups: the animals in group I served as the control group, while the animals in group II and group III were fed with hazelnut and canola oil, respectively. The testes of all rats were excised for histopathologic evaluation and immunohistochemical (IHC) evaluation with a standard method. Blood samples were obtained for determination of serum hormone levels. Results: No significant differences were noted with respect to behavior or weight among the three groups. Rats in the canola oil group (group III) had higher luteinizing hormone (LH) and higher testosterone levels than rats in the control group. Rats who received hazelnut oil (group II) exhibited similar findings, with these levels being higher than they were in the control group. No statistical differences were shown for histopathology or IHC testosterone antibody levels across all treatment groups. Conclussion: Canola oil was shown to have a greater effect on serum LH and testosterone compared to the control group and the group fed with hazelnut oil. Further investigation is required into how these oils affect serum hormone and sperm activity.Öğe The investigation of the antioxidative properties of the novel synthetic organoselenium compounds in some rat tissues(Sage Publications Ltd, 2008) Talas, Zeliha Selamoglu; Ozdemir, Ilknur; Yilmaz, Ismet; Gok, Yetkin; Orun, IbrahimDMBA (7,12-dimethylbenz[a]anthracene) is a polycyclic aromatic hydrocarbon (PAH) known to cause tumors in rats. Selenium is an essential element with physiological nonenzymatic antioxidant properties. Because of the health problems induced by many environmental pollutants, many efforts have been undertaken in evaluating the relative antioxidant potential of selenium and synthetic organoselenium compounds. In this study, adult female Wistar rats were treated with DMBA and the novel organoselenium compounds (1-isopropyl-3-methylbenzimidazole-2-selenone [Sel] and 1,3di-p-methoxybenzylpyrimidine-2-selenone [Sell]) in the determined doses. The protective effects of novel synthetic organoselenium compounds (Sel and Sell) against DMBA-induced changes in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) activities and total glutathione (GSH) and malonedialdehyde (MDA) levels of rat heart and brain were investigated. It was determined that Sel and Sell fully or partially restored enzyme activity. It was also found that lipid peroxidation was also decreased in Sel and Sell treated groups. Consequently, it was determined that novel synthetic organoselenium compounds (Sel and Sell) provided protection of antioxidant activity, and protection against lipid peroxidation measured as MDA in Sel and Sell treated groups was provided by novel synthesized organoselenium compounds. The ability of the organoselenium compounds to prevent oxidative damage induced by DMBA in rats was rationalized.
