Yazar "Yuce, Hande" seçeneğine göre listele
Listeleniyor 1 - 14 / 14
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe 18?-glycyrrhetinic acid attenuates global cerebral ischemia/reperfusion-induced cardiac damage in C57BL/J6 mice(Univ Sao Paulo, Conjunto Quimicas, 2022) Turkmen, Nese Basak; Yuce, Hande; Taslidere, Asli; Sahin, Yasemin; Ayhan, Idris; Unuvar, Songuel; Ciftci, OsmanThe aim of the present study is to investigate the cardioprotective effects of 1813-glycyrrhetinic acid (1813-GA) against oxidative and histological damage caused by global cerebral ischemia/ reperfusion (I/R) in C57BL/J6 mice. All male mice (n:40) were randomly divided into four groups: (1) sham-operated (Sham), (2) I/R, (3) 1813-GA, and (4) 1813-GA+I/R. Ischemia was not applied to the sham and 1813-GA groups. In the I/R group, the bilateral carotid arteries were clipped for 15 min to induce ischemia, and the mice were treated with the vehicle for 10 days. In the 1813-GA group, the mice were given 1813-GA (100 mg/kg) for 10 days following a median incision without carotid occlusion. In the 1813-GA+I/R group, the ischemic procedure performed to the I/R model was applied to the animals and afterwards they were intraperitoneally (i.p.) treated with 1813-GA (100 mg/kg) for 10 days. It was found that global cerebral I/R increased TBARS levels and decreased antioxidant parameters. The 1813-GA treatment decreased the level of TBARS and increased GSH, GPx, CAT, SOD activities. Also, the control group cardiac tissue samples were observed to have a normal histological appearance with the Hematoxylin-Eosin staining method. Histopathological damage was observed in the heart tissue samples belonging to the I/R group. The 1813-GA treatment ameliorates oxidative and histological injury in the heart tissue after global ischemia reperfusion, and may be a beneficial alternative treatment.Öğe Comparison of preoperative serum neopterin, periostin, indoleamine 2,3-dioxygenase, YKL-40, and tenascin-C levels with current tumor markers for early-stage endometrial cancer(Wiley, 2021) Unuvar, Songul; Melekoglu, Rauf; Turkmen, Nese B.; Yilmaz, Ercan; Yasar, Seyma; Yuce, HandeObjective: To compare the predictive value of serum levels of neopterin, periostin, YKL-40, tenascin-C (TNC), and indoleamine 2,3-dioxygenase (IDO) with current tumor markers for the primary diagnosis of early-stage endometrial cancer. Methods: A prospective cross-sectional study was conducted between January 2020 and November 2020. A total of 59 patients (38 women newly diagnosed with early-stage endometrial cancer [study group] and 21 women with benign endometrial pathologies [control group]) were enrolled. Blood samples were collected prior to surgery and underwent immunoassay analysis. Results: Carcinoembryonic antigen (CEA), periostin, and IDO levels were significantly higher in the study group than the control group (P = 0.008, P = 0.034, and P = 0.003, respectively). Receiver operating characteristic curve analysis revealed that IDO, periostin, and CEA were good predictors of early-stage endometrial cancer (AUC = 0.733, 95% CI, 0.602-0.840, P < 0.002; AUC = 0.668, 95% CI, 0.533-0.785, P = 0.018; and AUC = 0.709, 95% CI, 0.576-0.820, P = 0.002, respectively). Correlation analysis revealed no significant correlation of any biomarker with age or body mass index in either the control or study group. Conclusion: Serum CEA, periostin, and IDO levels were significantly higher in women with endometrial cancer than in those without cancer. These results may help identify new markers for diagnosing endometrial cancer.Öğe Current Pandemic in the World: Monkeypox from Past to Present(Acad Brasileira De Ciencias, 2023) Sahin, Yasemin; Yuce, Hande; Unuvar, Songul; Ciftci, OsmanMonkeypox is a zoonotic viral infection that was first identified in humans in 1970 in the Democratic Republic of Congo. The cases seen again in early May 2022 have reached 78.000 as of today. On July 23, 2022, the World Health Organization decided that the monkeypox outbreak represents a public health emergency. For the early diagnosis and effective treatment of monkeypox, inter-individual transmission routes, disease symptoms, factors affecting the course of the disease, presence of another infection, prognosis, pharmacological agents used in the prophylactic treatment, and their effects, populations at risk, waste disposal protocol should be known. For this reason, our aim is to reveal the sources of transmission of the monkeypox virus from past to present, what are the signs and symptoms in patients after infection, ways of protection from the virus, the mutation status of the virus, and treatment approaches.Öğe Dose dependent cytotoxic activity of patulin on neuroblastoma, colon and breast cancer cell line(2021) Turkmen, Nese Basak; Yuce, Hande; Ozek, Dilan Askin; Aslan, Sumeyye; Yasar, Seyma; Unuvar, SongulAim: Patulin, a mycotoxin, is an organic compound classified as a polypeptide. Patulin, which is generally detected in moldy fruits and their derivatives, has been suggested to have anticancer activity. Some studies have shown that it induces apoptosis in the cell. This study aims to investigate the anticancer activity of patulin in SH-SY5Y (human neuroblastoma cell line), HCT116 (human colon cancer cell line), and MCF-7 (human breast cancer cell line) cell lines. Materials and Methods: SH-SY5Y, HCT116, MCF-7, and L929 (healthy fibroblast) cell lines were used for cytotoxicity experiments. Cells were added in 96-well plates at 5x103 cells per well. Serial dilutions of patulin at a dose of 1, 2.5, 5, 10, 25, 50, and 100 µM were added to the waiting cells in 24 hours incubation. All cell lines were exposed to patulin for 24 and 48 hours. The cytotoxic activity of patulin in cancer and healthy cell lines was determined in vitro by the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium) cell viability test. The results of the toxicity tests were measured spectrophotometrically (450 nm) in ELISA at intervals of 24 hours for 2 days. Results: Patulin caused cytotoxic activity in all cell lines at a concentration of 100 µM. Patulin showed cytotoxic activity at low doses only in the SH-SY5Y cell line. At doses of 25 and 50 µM, HCT116 caused more than 50% death in the cell line, while higher concentrations induced cell death in the MCF-7 cell line. Conclusion: Patulin showed anticancer activity at high concentrations in colon and breast cancer cell lines, and both low and high concentrations in the SH-SY5Y cell line. Patulin may be a new candidate molecule in the treatment of neuroblastoma, colon, and breast cancers, depending on the dose.Öğe Evaluation of Lipocalin-2 and Metalloproteinase-9 Gene Expressions in Early-Stage Endometrial Cancer(Wiley, 2023) Unuvar, Songul; Melekoglu, Rauf; Bulut, Ezgi; Dogan, Aysegul; Turkmen, Nese Basak; Yuce, Hande; Yilmaz, Ercan[Abstract Not Available]Öğe Evaluation of NMR Results of Newly Synthesized Platinum-Based N- Heterocyclic Carbene Complexes(Wiley, 2023) Aslan, Sumeyye; Yuce, Hande; Yasar, Sedat; Bugday, Nesrin; Turkmen, Nese Basak; Eke, Benay Can; Unuvar, Songul[Abstract Not Available]Öğe Evaluation of serum neopterin, periostin, Tenascin-C, tissue inhibitor of metalloproteinase-1 and matrix metalloproteinase-2 levels in obese pregnant women(Galenos Publ House, 2022) Melekoglu, Rauf; Unuvar, Songul; Turkmen, Nese Basak; Cetin, Asli; Celik, Nesibe Zeyveli; Yuce, Hande; Yasar, SeymaObjective: To investigate the role of extracellular matrix proteins in the molecular mechanism of inflammatory response in obese pregnant women by comparing serum levels of neopterin, periostin, Tenascin-C, tissue inhibitor of metalloproteinase-1, and matrix metalloproteinase-2 between obese and normal weight pregnant women in the third trimester. Materials and Methods: A prospective cross-sectional study was conducted between April 2021 and December 2021. A total of 84 pregnant women were included and three groups were formed with 28 participants in each group. Results: Serum levels of neopterin, periostin, Tenascin-C and tissue inhibitor of metalloproteinase-1 were significantly higher in class II-III obese pregnant women than in class I obese and normal-weight women (p=0.002, p<0.001, p<0.001, and p<0.001, respectively). There was no significant difference in serum matrix metalloproteinase-2 levels between the groups (p=0.769). Receiver operating characteristic curve analysis showed that Tenascin-C and periostin were effective in predicting pre-eclampsia [area under the curve (AUC)=0.82, 95% confidence interval (CI), 0.72-0.90, p<0.001 and AUC=0.71, 95% CI, 0.60-0.80, p=0.007, respectively]. Conclusion: This study demonstrated that class II-III obese pregnant women had significantly higher serum levels of neopterin, periostin, Tenascin-C, and tissue inhibitor of metalloproteinase-1 in the third trimester. These higher serum levels may be associated with the adverse perinatal effects of obesity during pregnancy.Öğe Evaluation of the Cytotoxic Activity of Taurine on Hepatoma and Neuroblastoma Cell Lines(Wiley, 2023) Yuce, Hande; Sahin, Yasemin[Abstract Not Available]Öğe Evaluation of the Relationship Between Serum Metallothionein and Trace Element Levels in Multiple Sclerosis Patients(Wiley, 2023) Yuce, Hande; Tecellioglu, Mehmet; Tanbek, Kevser; Yasar, Seyma; Turkmen, Nese Basak; Ates, Ilker; Unuvar, Songul[Abstract Not Available]Öğe Investigation of the Protective Effect of Nerolidol on Dehydroepiandrosterone-induced Polycystic Ovary Syndrome in Female Rats(Wiley, 2023) Yuce, Hande; Turkmen, Nese Basak; Aydin, Muhterem; Taslidere, Asli; Dogan, Aysegul; Ozek, Dilan Askin; Hayal, Taha Bartu[Abstract Not Available]Öğe Molecular docking and in vitro anticancer studies of silver(I)-N-heterocyclic carbene complexes(Elsevier Sci Ltd, 2022) Akkoc, Mitat; Khan, Siraj; Yuce, Hande; Turkmen, Nese Basak; Yasar, Seyma; Yasar, Sedat; Ozdemir, IsmailA series of symmetric and unsymmetrical benzimidazolium-based N-heterocyclic carbene (NHC) precursors (1a-i) and their silver complexes (2a-i) have been synthesized. The Ag(I)-NHC complexes were characterized by H-1, C-13 {H-1} NMR, FTIR, LC/MS-QTOF, and elemental analysis. Anticancer and cytotoxic activity of all Ag(I)-NHC complexes were tested against healthy fibroblast cell line (L929), breast cancer cell line (MCF-7), and neuro-blastoma cell line (SH-SY5Y) by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4sulfo-phenyl)-2H-tetrazolium] assay. The 2b, 2c, 2e, 2g, 2h, and 2i complexes showed higher cytotoxicity than cisplatin against SH-SY5Y and MCF-7 and lower cytotoxic activity against L929 cell lines. Because of their high cytotoxic activity against cancer cells and low cytotoxicity against healthy fibroblast cell lines, the 2b, 2c, 2e, 2g, 2h, and 2i are expected to be new lead compounds. In addition, molecular docking studies were performed to explore the binding interactions of silver complexes with the enzyme to explore new anticancer compounds. Furthermore, ADME properties of all complexes were predicted to explore lead-like characteristics and may be a potential drug candidate for cancer treatment.Öğe Nerolidol attenuates dehydroepiandrosterone-induced polycystic ovary syndrome in rats by regulating oxidative stress and decreasing apoptosis(Pergamon-Elsevier Science Ltd, 2023) Turkmen, Nese Basak; Yuce, Hande; Aydin, Muhterem; Taslidere, Asli; Dogan, Aysegul; Ozek, Dilan Askin; Hayal, Taha BartuAims: Although nerolidol (NRL) is a naturally occurring sesquiterpene alcohol with many pharmacological ac-tivities, its role in dehydroepiandrosterone DHEA-induced polycystic ovary syndrome PCOS is unknown. This study aims to explore the potential beneficial effects and underlying molecular mechanisms of nerolidol treat-ment on polycystic ovary syndrome.Main methods: Pre-pubertal female Sprague-Dawley rats were randomly assigned into four groups (n = 8/group); group I: control; group II: PCOS; group III: P + NRL; group IV: NRL. Biochemical parameters related to oxidative stress, inflammation, apoptosis, and hormones were estimated in the blood and ovarian tissues. Histopatho-logical, ultrastructural, and immunohistochemical analyses were performed. Bax, P53, Cas-3, and Bcl-2 gene expression levels were detected with RT-PCR. The membrane array analysis detected chemokine, cytokine, and growth factor protein profiles.Key findings: In light of the available data, it can deduce that nerolidol has a significant ameliorating effect on lipid peroxidation, oxidative stress, inflammation, histopathological damage, and apoptosis accompanying PCOS in female rats.Significance: PCOS is not only a reproductive pathology but also a systemic condition and its etiopathogenesis is still not fully understood. Since changes in PCOS have important long-term effects on health, this study evaluated the efficacy of nerolidol, a phytotherapeutic for the control of biochemical, apoptotic, histopathological, and metabolic changes.Öğe Protective effect of resveratrol against pembrolizumab-induced hepatotoxicity and neurotoxicity in male rats(Wiley, 2023) Turkmen, Nese B.; Yuce, Hande; Sahin, Yasemin; Taslidere, Asli C.; Ozek, Dilan A.; Unuvar, Songul; Ciftci, OsmanThe present study investigates the effects of resveratrol (RSV) on brain and liver tissues in rats with pembrolizumab (PEMB)-induced toxicity. Obtained for the study were 28 male Sprague-Dawley rats (3-4 months old) which were divided into four groups: Group 1: Control. Group 2: Administered PEMB at 5 mg/kg/day i.p. for a week. Group 3: Administered RSV orally at the dose of 20 mg/kg/day for 30 days by gavage. Group 4: Administered PEMB and RSV at 20 and 5 mg/kg/day RSV, respectively, for 30 days. The results of this study revealed that PEMB leads to a significant increase in thiobarbituric acid reactive substance (TBARS) levels and a significant decrease in glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD) activities, and glutathione (GSH) levels in the liver and brain tissues. The decreased SOD, CAT, GPx activities, and GSH levels increased significantly following RSV treatment in Group 4. The PEMB treatment showed histopathological alterations associated with strong positive cysteinyl aspartic acid-protease-3 (caspase-3) immunoreactivity, while RSV treatment reduced both the expression of caspase-3 protein and the histopathological changes. RSV administration prevents the biochemical, immunological, and histological alterations induced by PEMB. It can be suggested that the lower caspase-3 immunoreactivity in the PEMB + RSV group than in the PEMB group led to an inhibition of RSV on apoptosis.Öğe The usefulness of biomarkers in diagnosis of asbestos-induced malignant pleural mesothelioma(Sage Publications Ltd, 2021) Tanriverdi, Zubeyde; Meteroglu, Fatih; Yuce, Hande; Senyigit, Abdurrahman; Iscan, Mumtaz; Unuvar, SongulIntroduction: Malignant pleural mesothelioma (MPM) is a malignant tumor that is associated mostly with asbestos exposure. The present study was to evaluates the diagnostic value of neopterin, periostin, YKL-40, Tenascin-C (TNC), and Indolamine 2,3-dioxygenase (IDO) as noninvasive markers of malign pleural mesothelioma. Methods: Included in the study were 30 patients diagnosed with malign pleural mesothelioma, and 25 people as a control group. Biomarker levels were determined using an enzyme immunoassay . A Mann-Whitney U test and Spearman correlation methods were used for the statistical analysis. Results: All evaluated biomarkers were found to be significantly higher in the MPM group than in the control group (p < 0.05). There was no effect of such variables as gender, age or MPMsubtype on the parameters (p > 0.05) in the patient group. All biomarkers were positively correlated with each other (p < 0.001). Conclusions: The current non-invasive biomarkers that can be used in the diagnosis of MPM yielded significant results and can make important contributions to the early diagnosis of MPM.
