Tıp Fakültesi, Tıbbi Biyoloji ve Genetik Anabilim Dalı Koleksiyonu
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Öğe The effects of acrylamide and vitamin E on kidneys in pregnancy: An experimental study(Taylor and Francis Ltd, 2018) Aksungur, Zeynep; Gül, Mehmet; Yiğitcan, Birgül; Çanta, Harika Gozukara; Altınöz, E.; Türköz, YusufObjectives: The objective of this study is to investigate possible damages to kidney tissues of pregnant rats and their fetuses exposed to acrylamide during pregnancy and possible protective effects of vitamin E against these damages. Material and methods: Rats were randomly assigned to five groups of control, corn oil, vitamin E, acrylamide, vitamin E + acrylamide, six pregnant rats in each. Mother and fetal kidney tissues were examined for malondialdehyde (MDA), reductase glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), total antioxidant status (TAS), total oxidant status (TOS), urea, creatine, trace elements such as Zn and Cu in the serum and histopathological analyses were conducted. Results: It was determined that acrylamide, administered during pregnancy, statistically significantly increased MDA and TOS levels, maternal serum urea, creatinine, and Zn levels, while it decreased GSH, TAS, SOD, and CAT levels (p ≤ .05) when compared with all other groups in the kidney tissues of pregnant rats and their fetuses and caused tubular degeneration, hemorrhage, narrowing, and closure in Bowman’s space, and, in the E vitamin group, it statistically significantly increased GSH, TAS, SOD, CAT, urea, creatinine, and Zn levels when compared with other groups and lowered TOS and MDA levels to those of the control group (p < .05) and there were no differences between the groups histologically. Conclusion: It was observed that acrylamide administered during pregnancy caused oxidative stress in kidney tissues of mother rats and their fetuses, resulting in tissue damage, and vitamin E application, which is considered to be a powerful antioxidant, inhibited oxidative stress.Öğe Protective effects of hypericum perforatum and quercetin in a rat model of ıschemia/reperfusionınjury of testes(GEORG THIEME VERLAG KG, RUDIGERSTR 14, D-70469 STUTTGART, GERMANY, 2018) Aksungur, ZeynepPurpose This study aimed to compare the protective effects of Hypericum perforatum (Hp) and quercetin, a flavonoid, against ischemia/reperfusion (I/R) injury in rat testes. Materials and Methods This study included 28 male Wistar albino rats that were divided into four groups. Except for the sham group, torsion was created by rotating both testes at an angle of 720 degrees clockwise for 2 hours. The Hp and quercetin groups received 25 mg/kg Hp and quercetin intraperitoneally 30 minutes before detorsion, respectively. Orchiectomy was performed for the measurement of markers of oxidative stress and histopathological examination. Results In the Hp and quercetin groups, malondialdehyde (MDA) and nitric oxide (NO) levels and total oxidant capacity were significantly lower, the glutathione level and total antioxidant status were significantly higher, and Johnsen's testis biopsy scores were significantly higher than in the torsion/detorsion group (p=0.001). The markers of oxidative injury were significantly lower (p=0.001) and total antioxidant status was significantly higher (p=0.001), except for glutathione (p=0.62) in the Hp group than in the quercetin group. Johnsen's score between Hp and quercetin groups was not significantly different (p=0.80). Conclusion Both Hp and quercetin have protective effects against I/R injury of the testes, but the protective effect of Hp was found to be stronger than that of quercetin.Öğe Prophylactic effect of somatostain on ERCP induced pancreatitis(Gazi Medical Journal, 1996) Görgül, Ahmet; Kayhan, Burçak; Kayhan, Başak; Menteş, Bülent; Aybay, Cemalettin; Cindoruk, Mehmet; Akçalı, Zafer; Dumlu, Şükrü; İmir, TurgutÖğe The effect of diphtheria toxin on nitric oxide induction from RAW264.7 murine macrophages(Turkish journal of medıcal scıences, 1997) Aybay, Cemalettin; Tarhan, Gülnur; İmir, Turgut; Kayhan, Başak: In this study the effect of diphtheria toxin (DT) on nitric oxide (NO) production from RAW 264.7 murine macrophages was investigated. Griess reagent was used to determine NO production by measuring nitrite levels in the culture supernatants. Corynebacterium diphtheriae G12/6 strain-produced DT (Limes flocculation activity and immunodiffusion assays were positive) demonstrated a dosedependent effect on RAW 264.7 macrophages to induce NO production. LNAME, an L-arginine analogue, inhibited NO production from DT-stimulated RAW 264.7 macrophages. At the given concentrations of DT, lipopolysaccharide (LPS or endotoxin)- induced NO production from RAW 264.7 macrophages was also inhibited. RAW 264.7 macrophage cells, incubated with various concentrations of DT for 3 days, were killed by DT in a dose dependent manner. Endotoxin contamination of DT was demonstrated with limulus amoebocyte lysate assay. Polymyxin B, frequently added to neutralize the effects of LPS in vitro, inhibited DT-induced NO production from RAW 264.7 cells. The misleading data concerning NO inducing capacity of DT was seemed to be related to endotoxin contamination. Thus, DT does not seem to be capable of inducing NO production from macrophages.Öğe HLA A B DRB1 allele and haplotype frequencies and comparison with blood group antigens in dialysis patients in the east anatolia region of Turkey(Transplantation proceedings, 2013) Kayhan, Başak; Kurtoğlu, Elçin Latıfe; Taşkapan, Hülya; Pişkin, Turgut; Şahin, İdris; Otlu, Gonca; Ünal, BülentAim. The first aim of that study was to investigate HLA class I and class II allele and haplotype frequencies in renal dialysis patients who live in East Anatolia in Turkey. Our second aim was to investigate whether there was a relationship between ABO and D blood group antigens and HLA alleles and haplotypes for the study group. Materials and methods. HLA class I and II polymorphisms in 408 renal dialysis patients were studied using sequence-specific primers (SSP) and sequence-specific oligonucleotides (SSO). Blood group antigens were detected by agglutination methods on microplates. Results. A total of 16 HLA-A, 34 HLA-B, and 15 HLA-DRB1 alleles were identified. The most frequent HLA-A alleles were HLA-A*02, HLA-A*24, and HLA-A*11. The most frequent HLA-B alleles were HLA-B*35, HLA-B*51, and HLA-B*44. In case of HLADRB1; HLA-DRB1*11, HLA-DRB1*04, and HLA-DRB1*13 were first 3 alleles with higher frequency, in order. In the combination of those 3 alleles, the most frequent HLAA-B-DRB1 haplotypes were HLA-A*02-B*51-DRB1*11, HLA-A*11-B*35-DRB1*11, A*24-B*35-DRB1*11. The frequency of ABO, D blood group antigens were observed as 0.168 for A Rh(þ), 0.019 for A Rh(), 0.057 for B Rh(þ), 0.013 for B Rh(), 0.123 for O Rh(þ), 0.014 for O Rh(), 0.018 for AB Rh(þ), and 0.001 for AB Rh(). While A Rh(þ) samples with HLA-A*02 and HLA-DRB1*11 had the highest frequencies (0.067 and 0.088, respectively), O Rh(þ) samples with HLA-B*51 had the highest frequency (0.06). Conclusion. According to haplotype frequencies HLA-A*02-B*51-DRB1*11 is also found at higher frequencies in Bulgarian and Armenian populations. In case of HLAassociated diseases, the east Anatolian population could be susceptible to myastenia gravis, Behçet’s disease, and systemic sclerosis due to the high frequencies of HLAA*24, HLA-B*51, and HLA-DRB1*11 respectively. We did not observe a correlation between blood group antigens and HLA alleles or haplotypes in renal dialysis patients.Öğe Effects of reciprocal interactions between various dietary fats and circadian phases on postprandial hyperlipidemia in rats(Biological Rhythm Research, 2017) Satılmış, Basri; Kayhan, Başak; Güldür, TayfunExpression levels of various intestinal proteins involved in postprandial lipoprotein assembly as well as plasma triglyceride concentration exhibit daily oscillations indicating circadian control. The length of the carbon chain and degree and position of unsaturation of fatty acids influence triglyceride secretion by the enterocytes. To this end, effects of reciprocal interactions of various single fats/oil (olive oil, fish oil or butter) gavaging either in active or passive phase were investigated in rats. Fat/oil gavaged in the active phase of circadian rhythm resulted in higher postprandial serum triglyceride levels compared to that in the passive phase. Moreover, olive oil led to higher MTP activity and apo B-48 gene expression, while fish oil gavaging caused more prominent apo B-48 and MTP gene expression when they were given in the passive phase. The present results indicate that circadian time at which fat or oil gavaged once might exert influence on postprandial lipoprotein synthesis/assembly.Öğe Thiol peroxidases mediate specific genome wide regulation of gene expression in response to hydrogen peroxide(Proceedings of the National Academy of Sciences, 2011) Fomenko, Dima; Koç, Ahmet; Agisheva, Natalia; Michael, Jacobsen; Kaya, Alaattin; Mikalai, Malinouski; Julian, Rutherford; Siu, Kam Leung; Yan Jin, Dong; Dennis, Winge; Vadim, GladysheHydrogen peroxide is thought to regulate cellular processes by direct oxidation of numerous cellular proteins, whereas antioxidants, most notably thiol peroxidases, are thought to reduce peroxides and inhibit H2O2 response. However, thiol peroxidases have also been implicated in activation of transcription factors and signaling. It remains unclear if these enzymes stimulate or inhibit redox regulation and whether this regulation is widespread or limited to a few cellular components. Herein, we found that Saccharomyces cerevisiae cells lacking all eight thiol peroxidases were viable and withstood redox stresses. They transcriptionally responded to various redox treatments, but were unable to activate and repress gene expression in response to H2O2. Further studies involving redox transcription factors suggested that thiol peroxidases are major regulators of global gene expression in response to H2O2. The data suggest that thiol peroxidases sense and transfer oxidative signals to the signaling proteins and regulate transcription, whereas a direct interaction between H2O2 and other cellular proteins plays a secondary role.Öğe Functional characterization of new mutations in Wilson disease gene ATP7B using the yeast model(J Trace Elem Med Biol., 2015) Papur, Özlenen Şimşek; Koç, Ahmet; Terzioğlu, OrhanThe Wilson disease gene, a copper transporting ATPase (Atp7b), is responsible for the sequestration of Cu into secretory vesicles, and this function is exhibited by the orthologous Ccc2p in the yeast. In this study, we aimed to characterize clinically relevant new mutations of human ATP7B (p.T788I, p.V1036I and p.R1038G-fsX83)in yeastlacking the CCC2 gene. Expression of human wild type ATP7B gene in ccc2 mutant yeast restored the growth deficiency and copper transport activity; however, expression of the mutant forms did not restore the copper transport functions and only partially supported the cell growth. Our data support that p.T788I, p.V1036I and p.R1038G-fsX83 mutations cause functional deficiency in ATP7B functions and suggest that these residues are important for normal ATP7B function.
