Investigation of bacterial pathogens in milk from mastitic dairy cattle by matrix-assisted laser desorption ionization-time of flight mass spectrometry

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dc.authoridTanriverdi, Elif Seren/0000-0002-0449-0356
dc.authoridSafak, Tarik/0000-0002-6178-4641;
dc.authorwosidRisvanli, Ali/JDD-9731-2023
dc.authorwosidTanriverdi, Elif Seren/ABE-4472-2021
dc.authorwosidSafak, Tarik/W-5052-2018
dc.authorwosidYAKUPOGULLARI, YUSUF/F-3966-2011
dc.contributor.authorOzbey, Gokben
dc.contributor.authorOtlu, Baris
dc.contributor.authorYakupogullari, Yusuf
dc.contributor.authorCelik, Betul
dc.contributor.authorTanriverdi, Elif Seren
dc.contributor.authorKelestemur, Neslihan
dc.contributor.authorSafak, Tarik
dc.date.accessioned2024-08-04T20:51:50Z
dc.date.available2024-08-04T20:51:50Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe scope of the present study was to assess the use of Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry (MS) as a quick technique for the identification of bacterial species in mastitis. In this study, milk samples from each udder quarter from a total of 250 dairy cattle were aseptically collected and tested. The samples were grouped into California Mastitis Test (CMT) positive, CMT negative and clinical mastitis. The samples were streaked on blood agar and the bacterial isolates were analysed using MALDI-TOF MS. Using MALDI-TOF MS, certain species such as Staphylococcus chromogenes (44/188, 23.4%), Aerococcus viridans (40/188, 21.3%) and Staphylococcus haemolyticus (19/188, 10.1%) were identified at a higher proportion in milk samples from cattle that were CMT positive. Moreover, the most common bacteria isolated from CMT negative milk samples were A. viridans (56/161, 34.8%), S. haemolyticus (24/161, 14.9%) and S. chromogenes (17/161, 10.6%). Only one isolate of S. chromogenes (1/4, 25%), A. viridans (1/4, 25%), S. haemolyticus (1/4, 25%) and Enterococcus faecium (1/4, 25%) was detected from milk samples with clinical mastitis using MALDI-TOF MS. There was a concurrence between the MALDI-TOF and biochemical bacterial identification method in 325 of 353 samples (92.06%). This study concludes that MALDI-TOF can be applied for quick determination of bacterial isolates once the bacterial colony has been isolated in milk samples.en_US
dc.identifier.doi10.14456/tjvm.2022.7
dc.identifier.endpage73en_US
dc.identifier.issn0125-6491
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85127542142en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage63en_US
dc.identifier.urihttps://doi.org/10.14456/tjvm.2022.7
dc.identifier.urihttps://hdl.handle.net/11616/100588
dc.identifier.volume52en_US
dc.identifier.wosWOS:000800835400001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherChulalongkorn Univen_US
dc.relation.ispartofThai Journal of Veterinary Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCattleen_US
dc.subjectCalifornia Mastitis Testen_US
dc.subjectMALDI-TOFen_US
dc.subjectmilk samplesen_US
dc.titleInvestigation of bacterial pathogens in milk from mastitic dairy cattle by matrix-assisted laser desorption ionization-time of flight mass spectrometryen_US
dc.typeArticleen_US

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